• Journal of Pharmaceutical Investigation
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• v.31 no.4
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• pp.241-256
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• 2001

Alginate microspheres, containing fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) or green fluorescent protein (GFP) were prepared and used as a model drug to develop the oral vaccine delivery system. The alginate microspheres were coated with poly-L-lysine or chitosan. Two methods, w/o-emulsion and spray, were used to prepare alginate microspheres. To optimize preparation conditions, effects of several factors on the particle size and particle morphology of microsphere, and loading efficiency of model antigen were investigated. In both preparation methods, the particle size and the loading efficiency were enhanced when the concentration of sodium alginate increased. In the w/o-emulsion preparation method, as the concentration of Span 80 was increased from 0.5% to 2%, the particle size was decreased, but the loading efficiency was increased. The higher the emulsification speed was, the smaller the particle size and loading efficiency were. The concentration of calcium chloride did not show any effect on the particle size and loading efficiency. In the spray preparation method, the particle size was increased as the nozzle pressure $(from\;1\;kgf/m^2\;to\;3\;kgf/m^2)$ and spray rate was raised. Increasing calcium chloride concentration (<7%) decreased the particle size, in contrast to no effect of calcium chloride concentration on the w/o-emulsion preparation method. Alginate microspheres prepared by two methods were different in the particle size and loading efficiency, the particle size of microspheres prepared by the spray method was about $2-6\;{\mu}m$, larger than that prepared by the w/o emulsion method $(about\;2{\mu}m)$, and the loading efficiency was also higher with spray method. Furthermore, drying process for the microspheres prepared by the spray was simpler and easier, compared with the w/o emulsion preparation. Therefore, the spray method was chosen to prepare alginate microspheres for further experiments. Release pattern of FITC-BSA in alginate microspheres was evaluated in simulated intestinal fluid and PBS (phosphate buffered saline). Dissolution rate of FITC-BSA from alginate/chitosan microsphere was lower than that from alginate microsphere and alginate/poly-L-lysine microsphere. By confocal laser scanning microscope, it was revealed that alginate/FITC-poly-L-lysine microspheres were present in close apposition epithelium of the Peyer's patches of rabbits following inoculation into lumen of intestine, which proved that microspheres could be taken up by Peyer's patch. In conclusion, it is suggested that alginate microsphere prepared by spray method, showing a particle size of & $10\;{\mu}m$ and a high loading efficiency, can be used as a model drug for the development of oral vaccine delivery system.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.2
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• pp.123-129
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• 2017

Objectives: The purpose of this study is to enhance the application of analytical method of polar solvents(alcohols) by GCOTC (gas chromatography open tubular column) through the system suitability test(SST) to estimate the whole chromatographic system performance(integral part). Methods: To perform the SST, carried out repeatability(n=6) as analytical method of polar solvents by GCOTC, got the retention time($t_R$), standard deviation(${\sigma}_{n-1}$) of $t_R$, baseline width($w_b=4{\sigma}_{n-1}$) and calculated dead time($t_m$) by $v_m=d^2{\pi}L(f/4)$ and $v_m=t_m$ x flow rate. Results: In this experiment, obtained the basic data, there were $t_m=2$ min, methanol($t_R=3.569$, ${\sigma}_{n-1}=0.01$, $w_b=0.04$), ethanol ($t_R=3.892$, ${\sigma}_{n-1}=0.004$, $w_b=0.016$), isopropanol($t_R=4.209$, ${\sigma}_{n-1}=0.004$, $w_b=0.016$). By using these data, calculated the corrected retention time($t_R{^{\prime}}$), capacity factor(k), separation factor(${\alpha}$), number of theoretical plate(n) and resolution($R_s$) for SST and got the good results. Conclusions: Through the SST, could reconfirm the whole chromatographic performance system(integral part) for analytical method of polar solvents by GCOTC. Therefore, this analytical method expect to be widely applied at the related areas.

• Journal of the Korean Institute of Telematics and Electronics C
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• v.36C no.6
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• pp.37-43
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• 1999

Two-way addressing method has been proposed for efficient VLSI implementation of Euclidean calculation circuit for pipelined Reed-Solomon decoder. This new circuit is operating with single clock while exploiting maximum parallelism, and uses register addressing instead of register shifting to minimize the switching power. Logic synthesis shows the circuit with the new scheme takes 3,000 logic gates, which is about 40% reduction from the previous 5,000 gate implementation. Computer simulation also shows the power consumption is about 3mW. The previous implementation with multiple clock consumed about 5mW.

• 전기의세계
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• v.20 no.1
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• pp.9-18
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• 1971

The characteristics of optimum control for the second order P.W.M. sampled-data system with linear plants are investigated, with the development of method for finding the cannonical represetations for the initial states, as well as the optimality of modulator output for the P.W.M. system, which contains the electro-mechanical components, by dividing the phase plane into several regions, in which the position of the system in the phase plane are converted into the pulse polarity and pulse width.

• Journal of the Korean Institute of Telematics and Electronics
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• v.20 no.6
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• pp.11-17
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• 1983

In this paper, the application of the explicit M.R.A.C. algorithms to the D.C. motor speed control using the microprocessor is studied. The adaptation algorithms are derived from the gradient method and the exponentially weighted least square [E.W.L.S.] method. In order to minimize the computational instability of the E.W.L.S. method, the adaptation algorithm of UDUt factorization method is developed, and because of the characteristics of the D.C. motor (dead-aone phenomenon) , the SM. gra-dient type algorithm is also improved from the gradient type algorithm. Computer simulations and experiments show that these algorithms adapt well to the rapid change of the reference input and the load.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.94-94
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• 2021

현재 전 지구적으로 일어나고 있는 극단적 기후현상과 이로 인한 자연재해의 원인은 복합적이다. 기후변화로 인한 영향과 동시에 도시화 또한 하나의 원인으로 작용하고 있다. 이러한 영향을 완화하기 위한 방안으로 도시의 그린인프라와 저영향개발은 최근 지속가능한 발전을 위해 꼭 필요한 요소로 자리잡고 있다. 그린인프라 유형 중 하나인 옥상녹화는 많은 이점을 제공한다. 도시에 위치한 건물의 상층부, 내부 및 주변 온도을 낮춤으로써 얻을 수 있는 에너지절감 효과와 강우 시 상당량의 빗물을 저류함으로써 기대되는 우수유출 저감효과, 그리고 도시 공간 내 식물의 적극적인 도입으로 인한 이산화탄소 배출 저감 효과 등을 기대할 수 있다. 본 연구에서는 도시지역의 열섬현상완화와 유출저감의 방안 중 하나인 옥상녹화(Green roof)의 효과를 정량적으로 평가하기 위해 콘크리트로 이루어진 동일한 제원의 실험동을 구축하고, 실험동 내외부의 연직방향 온도, 습도, 강우, 풍속, 일사량 등의 기상자료를 측정할 수 있는 센서를 설치하였다. 각 실험동에서 측정된 기상자료를 Flux Profile Method를 적용하여 무강우기간과 강우발생기간 동안의 연직 방향의 현열속, 잠열속, 토양열속(H, LE, G) 을 산정하였다. 에너지 평형에 따라 산정된 각 실험동의 열속과 지표면 복사량 관측자료을 정량적으로 비교하여 적용성을 평가하였다. 실험의 대조군인 일반 코팅재로 마감된 콘크리트 지붕의 무강우 기간 중 최대 현열속 693.82 W/m² 잠열속은 330.15 W/m² 으로 나타났으며, 실험군인 옥상녹화가 조성된 지붕의 최대 현열속 436.27 W/m², 잠열속 949.20 W/m² 으로 나타났으며, 산정치와 관측치 시계열의 NSE는 0.81 으로 Flux Profile Method를 통해 산정된 열속의 정확도는 비교적 높은 것으로 나타났다. 이와 같은 방법으로 옥상녹화의 정량적 평가가 가능해짐으로써 향후 기후변화 대응방안 및 전략 수립 시 옥상녹화의 온도저감효과 분석에 적극 활용할 수 있을 것으로 판단된다.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.819-826
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• 2014

This study was carried out to investigate the effects of night-time far-infrared irradiance quality of red pepper dried in greenhouses. This study involved 4 treatments: sunlight alone (control), or sunlight plus nightly far-infrared irradiation at $250W{\cdot}6.6m^{-2}$ ($250W{\cdot}6.6m^{-2}$), far-infrared irradiation at $250W{\cdot}3.3m^{-2}$ ($250W{\cdot}3.3m^{-2}$), or far-infrared irradiation $500W{\cdot}3.3m^{-2}$ ($500W{\cdot}3.3m^{-2}$). The drying periods were 12 days in $500W{\cdot}3.3m^{-2}$ and $250W{\cdot}3.3m^{-2}$, and 14 days in $250W{\cdot}6.6m^{-2}$, and 15 days in the control. The daytime temperature was same among the treatments. The lowest temperature was at $23.8^{\circ}C$ in control, and $29.5-37.2^{\circ}C$ in far-infrared irradiation treatments. The marketable yield was 7-14% higher in far-infrared irradiation treatments compared to the control. The rate of marketability was higher in far-infrared irradiation treatments (93.6-96.3%) than in the control (87.0-87.5%). The American Spice Trade Association (ASTA) value was greatest in the $250W{\cdot}3.3m^{-2}$ treatment, followed by $250W{\cdot}6.6m^{-2}$, then $500W{\cdot}3.3m^{-2}$, and finally the control. Capsaicinoid content showed no regular trend among the treatments. Our results provide an optimized method for reducing drying time of red pepper under sunlight, and improving the quality of dried red pepper.

• 한국신재생에너지학회:학술대회논문집
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• 2005.11a
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• pp.711-711
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• 2005

• Mass Spectrometry Letters
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• v.3 no.2
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• pp.58-61
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• 2012

A method based on flow injection-isotope dilution-cold vapor-inductively coupled plasma mass spectrometry (FI-IDCV-ICP/MS) has been applied to determine trace level of mercury in fly ash. $^{200}Hg$ isotopic spike was added to 0.25 g of BCR176R fly ash and then decomposed by microwave digestion procedure with acid mixture A (8 mL $HNO_3$ + 2 mL HCl + 2 mL HF) and acid mixture B (8 mL $HNO_3$ + 2 mL $HClO_4$ + 2 mL HF) for applying IDMS. Mercury cold vapor was generated by using reductant solution of 0.2% (w/w) $NaBH_4$ and 0.05% (w/w) NaOH. The measurements of n($^{200}Hg$)/n($^{202}Hg$) isotope ratio was made using a quadrupole ICP/MS system. The accuracy in this method was verified by the analysis of certified reference material (CRM) of fly ash (BCR 176R). The indicative value of Hg in BCR 176R fly ash was $1.60{\pm}0.23$ mg/kg (k = 2). The determined values of Hg in BCR 176R fly ash by the method of FI-CV-ID-ICP/MS described in this paper were $1.60{\pm}0.24$ mg/kg (k = 3.18) and the analysis results were in well agreement with the indicative value within the range of uncertainty.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.160-163
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• 2004

In domestic, various repair materials and method systems to keep up with these reinforced concrete deteriorated due to salt damage, carbonation, chemical decay et. developed and applied. However, on-site quality control of various repair materials and method systems isn't achieved desirably because it is depend completely on a men of experience' opinions above all else regardless of various on-site environments. In this background, mock up test with due regard to real on-site environments was performed to secure fundamental data for establishment of desirable on-site quality control. Mock up test using repair mortar analyzed from angles of construction methods, mechanical spraying pressures, W/M. Construction methods were designed manpower method and spraying method, spraying pressures were designed 32, 42, 52 psi, W/M were designed 14.4, 15.4, $16.4\%$. And compressive strength, Chloride ion diffusion coefficient, bond strength, SEM. of mock up test specimens were evaluated. In conclusion, we confirmed excellency of mechanical spraying pressures, fined extremely excellency of condition of spraying pressure 42 ps, W/M $14.4\%$ within this study. therefore the results of this study will be useful to provide fundamental data for establishment of desirable on-site quality control.