• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1620-1632
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• 2017

Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of myosin heavy chain (MyHC) gene expression in myocytes. Methods: In a completely randomized design, ninety pigs were assigned to three diets with five replications per treatment and six pigs per pen. The diets included the basal diet (control group), TCMF1 (basal diet+2.5 g/kg TCMF) and TCMF2 (basal diet+5 g/kg TCMF). The psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining. Cell proliferation was measured using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) dye and cytometry. Isoforms of MyHC gene expression were detected by real-time quantitative polymerase chain reaction. Results: The final body weight and carcass weight of finishing pigs were increased by TCMF1 (p<0.05), while the psoas major muscle cross-sectional area was increased by TCMF (p<0.05). The cross-sectional area and diameter of psoas major muscle fiber Ι, IIA, and IIB were increased by TCMF2 (p<0.05). The cross-sectional area and fiber diameter of psoas major muscle fiber IIA and IIB were increased by diet supplementation with TCMF1 (p<0.05). Psoas major muscle fiber IIA and IIB fiber density from the pigs fed the TCMF1 diet and the type IIB fiber density from the pigs fed the TCMF2 diet were lower compared to pigs fed the control diet (p<0.05). Pigs fed TCMF2 had a higher composition of type Ι fiber and a lower percentage of type IIB fiber in the psoas major muscle (p<0.05). The expression levels of MyHC Ι, MyHC IIa, and MyHC IIx mRNA increased and the amount of MyHC IIb mRNA decreased in the psoas major muscle from TCMF2, whereas MyHC Ι and MyHC IIx mRNA increased in the psoas major muscle from TCMF1 (p<0.05). Peroxisome proliferator-activated receptor ${\gamma}$ $coactivator-1{\alpha}$ and CaN mRNA expression in the psoas major muscle were up-regulated by TCMF (p<0.05). Porcine skeletal muscle satellite cell proliferation was promoted by $4{\mu}g/mL$ and $20{\mu}g/mL$ TCMF water extraction (p<0.05). Both $1{\mu}g/mL$ and $5{\mu}g/mL$ of TCMF water extraction increased MyHC IIa, MyHC IIb, and MyHC IIx mRNA expression in porcine myocytes (p<0.05), while MyHC Ι mRNA expression in porcine myocytes was decreased by $5{\mu}g/mL$ TCMF water extraction (p<0.05). Porcine myocyte MyHC Ι and MyHC IIx mRNA expression were increased, and MyHC IIa and MyHC IIb mRNA expression were down-regulated by $5{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). MyHC Ι and MyHC IIa mRNA expression in porcine myocytes were increased, and the MyHC IIb mRNA expression was decreased by $1{\mu}g/mL$ TCMF ethyl acetate extraction (p<0.05). Four isoforms of MyHC mRNA expression in porcine myocytes were reduced by $5{\mu}g/mL$ TCMF petroleum ether extraction (p<0.05). MyHC IIa mRNA expression in porcine myocytes increased and MyHC IIb mRNA expression decreased by $1{\mu}g/mL$ in a TCMF petroleum ether extraction (p<0.05). Conclusion: These results indicated that TCMF amplified the psoas major muscle cross-sectional area through changing muscle fiber characteristics in finishing pigs. This effect was confirmed as TCMF extraction promoted porcine cell proliferation and affected isoforms of MyHC gene expression in myocytes.

• Korean Journal of Environmental Agriculture
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• v.16 no.4
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• pp.365-372
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• 1997

Biochemical characteristics and activities of glutathione-S-transferases(GSTs) and carboxylesterase extracted from soybean and corn plants treated with quizalofop-ethyl were investigated. Km value and Vmax of GSTs extracted from soybean and corn plants were $6.7{\times}10^{-3}M$ nmole/mg/min, 50, 20 nmole/mg/min, respectively. Optimum pH of carboxylesterase from soybean and corn was 7.0. Km value and Vmax of carboxylesterase extracted from soybean and corn plants were $4.2{\times}10^{-4}M$, $2.5{\times}10^{-4}M$ nmole/mg/min, 33, 10 nmole/mg/min, respectively. GSTs and carboxylesterase activity were reduced by quizalofop-ethyl. GSTs and carboxylesterse activity of corn was more reduced than that of soybean. When soybean and corn were treated by 80 ppm of quizalofopethyl. Soybean recovered after 10 days elapsing, but corn withered after 3days elapsing.

• Kyungpook Mathematical Journal
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• v.63 no.2
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• pp.235-250
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• 2023

Let 1 ≤ p, q < ∞ be fixed, and let R = [r_jk] be an infinite scalar matrix such that 1 ≤ r_jk < ∞ and sup_j,k r_jk < ∞. Let 𝓑(𝑙_p, 𝑙_q) be the set of all bounded linear operator from 𝑙_p into 𝑙_q. For a fixed Banach algebra 𝐁 with identity, we define a new vector space S^R_p,q(𝐁) of infinite matrices over 𝐁 and a paranorm G on S^R_p,q(𝐁) as follows: let $$S^R_{p,q}({\mathbf{B}})=\{A:A^{[R]}{\in}{\mathcal{B}}(l_p,l_q)\}$$ and $G(A)={\parallel}A^{[R]}{\parallel}^{\frac{1}{M}}_{p,q}$, where $A^{[R]}=[{\parallel}a_{jk}{\parallel}^{r_{jk}}]$ and M = max{1, sup_j,k r_jk}. The existance of S^R_p,q(𝐁) equipped with the paranorm G(·) including its completeness are studied. We also provide characterizations of β -dual of the paranormed space.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.4
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• pp.381-400
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• 1985

Twelve species of subfamily Leuciscinae from Korea are reviewed and keys to species and genera are provided. The species include Tribolodon hakonensis, T. branti, Phoxinus phoxinus, Moroco oxycephalus, M. steindachneri, M. keumgang, M. semotilus, Zacco platypus, Z. temminckii, Opsariichthys uncirostris amurensis, Aphyocypris chinensis and Squaliobarbus curriculus. In the present study M. lagowskii and O. bidens recorded previously in Korea have to be classified to M. steindachneri and O.u.amurensis based on several characters such as caudal peduncle depth, their distributions and lateral line scales, etc. A. chinensis from Korea is easily distinguished from that of mainland China in head length and caudal peduncle length. The skull and weberian apparatus of M. keumgang, endemir to Korea are described and compared with the other species of this subfamily. The diploid chromosome number of 9 species has three patterns: 2n=50 (T. hakonensis, P. phoxinus, M. oxycephalus, M. steindachneri and M. keumgang), 2n=48(Z. platypus, Z. temminckii and A. chinensis), 2n=76 (O. u. amurensis). Polyploidization or intraspecific polymorphism of chromosome was not observed in any species studied. Both species of P. phoxinus and A. chinensis are shown to be electrophoretically distinct from other taxa examined. The genera of subfamily Leuciscinae in Korea are represented by three possible groups: 1) Zacco-Opsariichthys-Tribolodon-Phoxinus-Morco, 2) Aphyocypris, and 3) Squaliobarbus.

• KSBB Journal
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• v.19 no.1
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• pp.17-26
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• 2004

In this study the extracellular production of 5-aminolevulinic aicd (ALA) by recombinant E. coli BL2l (DE3) pLysS harboring the plasmid pFLS45 are investigated. Optimum concentrations of succinic acid and glycine for cell growth and ALA production were found to be 30 mM and 15 mM, respectively. Levulinic acid (LA) as an inhibitor of ALAD was added to the culture medium in the end of exponential cell growth phase and its optimum concentration was 30 mM. Growth of recombinant E. coli BL2l (DE3) pLysS (pFLS45) was largely dependent upon the pH value of culture medium. When the pH of culture medium was in the range of 6.0 and 6.5, high cell mass and ALA production were obtained. IPTG induction for the expression of the fusion gene did not enhance the production of ALA. Recombinant cell grew at 30't faster than at 37$^{\circ}C$, but ALA productivity was lower than at 37$^{\circ}C$. Repeated addition of glycine, succinic acid, and LA increased the production of ALA and the inhibition of intracellular ALA dehydratase activity, with up to 1.3 g/L ALA having been produced in the cultivation.

• Journal of Korean society of Dental Hygiene
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• v.7 no.4
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• pp.569-582
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• 2007

The author aims at first securing basic material required for developing and operating proper oral health care education programs though making an investigation into the undergraduates of S College of Education on the actual status of oral health care and perceptions of oral health care education. The obtained results were as follows 1. In the actual status of oral care based on whether or not they have experiences in taking oral health care education, undergraduates with teeth brushing for more than three minutes a time were found to be 25.9% and 15.7% respectively according to the existence and nonexistence of experiences in the education(pM0.020), and undergraduates with teeth brushing in a circular motion was found to be 64.7% and 51.7% respectively, showing statistically significant differences(pM0.015). 2. In the actual status of preventative oral care according to whether or not they have experiences in taking oral care education, degrees of awareness of the usefulness of dental cleansers among the undergraduates were found to be 37.6% and 21.5% respectively according to the existence and nonexistence of experiences in the education(pM0.001), undergraduates with experiences in scaling were found to be 51.8% and 34.9% respectively(pM0.002) and undergraduates with experiences in having dental sealants were found to be 26.5% and 16.9%(pM0.031), showing statistically significant differences. 3. In the survey on perceptions of oral health care according to the existence and nonexistence of experiences in oral health care education, it was revealed that the case that they indicated 'the necessity of oral health care education' was 87.1% and 64.0% respectively according to the existence and nonexistence of the experience(pM0.000), and undergraduates intended to 'participate in oral care education for students(at mid and high schools and so on) after being teachers' were 77.6% and 65.7% respectively, showing statistically significant differences(pM0.011). 4. In general characteristics according to awareness of the necessity of cultivating oral health professionals, groups with awareness of the necessity consisted of 31.8% of freshmen and juniors(pM0.001), 55.69b of female undergraduates(pM0.001), 80.8% of non-smokers(pM0.012), 38.9% of large city residents(pM0.002) and 32.3% of undergraduates living in their own houses (pM0.028), showing statistically significant differences.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.1
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• pp.46-54
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• 1997

Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

• Journal of the Korean Chemical Society
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• v.36 no.4
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• pp.552-557
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• 1992

Chloramphenicol was studied by differential pulse polarography (DDP). A reduction peak which is dependent on pH of the solution appeared in the voltage range between zero and -1.50 volt vs. Ag/AgCl (sat. KCl) reference electrode. A plot of peak potentials (Ep) measured at room temperature (20$^{\circ}C$) vs. pH of the chloramphenicol solutions showed linear relationship changing slope (Ep/pH) at pH 8.9. The slope was -59.7 mV/pH in pH 2.7∼8.9 and -24.3 mV/pH in pH 8.9∼11.2, respectively. A log plot of peak currents (ip) vs. concentrations showed a linearity at the concentrations between 4.8 ${\times}$ 10$^{-7}$ M and 6.2 ${\times}$ 10$^{-5}$ M (0.16 ppm∼20 ppm) chloramphenicol in pH 8.0 ammonium buffer. Between the DPP method and the reference method measuring absorbance at 278 nm, the correlation coefficient was 0.996, which means an excellent linearity. The DPP method was able to detect degradation products of chloramphenicol in mild alkaline solution (pH = 8.0) more distinctly than the spectrophotometric method.

• Microbiology and Biotechnology Letters
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• v.18 no.3
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• pp.268-272
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• 1990

The structural gene (zadhII) encoding an alcohol dehydrogenase II from Zyrnornonas mobilis was cloned into Escherichia coli in our laboratory (Yoon et al., 1989. Kor. J. Microbiol. Biotechnol.). From E. coli (pADS93) carrying the zadhII gene, the Z mobilis alcochol dehydrogenase II (ZADH-II) was purified by sonication, $(NH_4)_2SO_4$, fractionation, and chromatography. The ZADH-I1 enzyme produced by Z. mobilis cell was also purified to compare to the enzyme produced by E. coli (pADS93). The purified enzyme from cell extract of E. coli (pADS93) was identified to be a tetramer being composed of four identical subunits having molecular weight of 40, 000 dalton like that of Z. mobilis. The pH optimum for the reaction oxidizing ethanol to acetaldehyde was 10.0 while the optimum for the reverse reaction was 7.5-8.5. The apparent $K_m$ values for ethanol and NAD + were $1.2 \times 10^{-1}M$and $5.1\times 10^{-5}M$, respectively. In addition, it was found that the $K_m$ value for acetaldehyde was very lower than that for ethanol.

• Ecology and Resilient Infrastructure
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• v.4 no.4
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• pp.216-225
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• 2017

We assessed the microhabitat conditions of three fish species (black shinner Pseudopungtungia nigra, striped shinner Pungtungia herzi, Korean shinner Coreoleuciscus splendidus) considering various stream order in the Geumgang River Basin. A total of 1,275 individuals were collected from 19 sites. And the microhabitat condition was assessed measuring flow velocity, water depth and substrate size at each site. The highest relative frequencies for P. herzi, C. splendidus and P. nigra were calculated 100% (n = 310), 78.9% (n = 784) and 47.3% (n = 181) respectively. P. herzi appeared in all streams, irrespective of their stream order; however, appearance in streams with the $2^{nd}$ order or lower was found to be scarcer than in the ones with the $3^{rd}$ order or higher. On the other hand, P. nigra and C. splendidus didn't appear in streams with the $2^{nd}$ order or lower. When it comes to P. nigra, the ones with 7 to 8 cm in length most frequently appeared. They inhabited streams, mainly, with 0.2 to $0.4m\;s^{-1}$ and 0.5 m in velocity and water depth, respectively. The three species all preferred runs as types of habitats with lots of coarse gravels-cobbles. P. herzi, the ones with 5 cm or shorter in length most frequently appeared. They inhabited streams, mainly, with 0 to $0.3m\;s^{-1}$ and 0.4 m in velocity and water depth, respectively. C. splendidus with 7 to 8 cm in length occupied the majority of them. They appeared in streams, mainly, with $0.9m\;s^{-1}$or higher and 0.3 m in velocity and water depth.