• 한국식품영양과학회지
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• 제39권10호
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• pp.1411-1417
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• 2010

총 폴리페놀 함량은 로즈마리에서 가장 높은 폴리페놀 함량을 보였고, 구절초, 라벤더, 레몬그라스도 $72{\sim}80\;{\mu}g$/mg의 비교적 높은 폴리페놀 함량을 보였다. 반면 카렌듈라와 목향은 다른 허브류보다 20~40% 정도의 낮은 폴리페놀의 함량을 보였다. 그리고 총 플라보노이드 함량에서는 레몬그라스, 로즈마리, 구절초, 카렌듈라, 라벤더, 목향 순으로 높은 함량을 나타냈다. 로즈마리, 라벤더, 구절초, 레몬그라스는 최고농도에서 90% 이상의 높은 DPPH 소거능이 있었고, $IC_{50}$값은 로즈마리 $6.23{\pm}0.58\;{\mu}g/mL$, 라벤더 $18.29{\pm}0.68\;{\mu}g/mL$, 구절초 $20.33{\pm}1.04\;{\mu}g/mL$, 레몬그라스 $22.38{\pm}0.77\;{\mu}g/mL$, 카렌듈라 $60.03{\pm}4.01\;{\mu}g/mL$, 목향 $65.64{\pm}2.64\;{\mu}g/mL$이었다. 이들 허브 추출물들의 항균 활성을 살펴보면, S. aureus 균주는 최고농도 100 mg/mL에서 구절초 14.5 mm, 로즈마리 15 mm로 가장 뛰어난 항균활성을 나타내었고, E. coli O157:H7 균주는 최고농도 100 mg/mL에서 구절초 16 mm, 로즈마리 17 mm로 우수한 항균효과를 나타냈다. 따라서 허브류 중에서 로즈마리 추출물이 가장 높은 총 폴리페놀의 함량을 보였고, 총 플라보노이드의 함량은 레몬그라스에서 가장 높았다. 또한 S. aureus와 E. coli O157:H7 2종류의 균에 대한 항균 효과는 로즈마리와 구절초에서 가장 뛰어난 효과를 확인하였다.

• 한국해양바이오학회지
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• 제14권2호
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• pp.102-111
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• 2022

Beakryoung-do is located in the north along the western coast of South Korea. Owing to its geographical limitations, not many studies have been conducted on marine algae in this region. This study aims to investigate the marine algal flora and the community structure in the intertidal and subtidal zones of Nampo-ri, Beakryoung-myeon, Ongin-gun, Incheon regions of South Korea. The study was conducted from August 2015 to June 2016 during all seasons. A total of 95 algal species, including 7 green algae, 12 brown algae, and 76 red algae were collected and identified; 52 algal species were found in the intertidal zone and 83 in the subtidal zone. The vertical distribution of algae from upper to lower intertidal zone was Neorhodomela aculeata - Caulacanthus okamurae, Gelidiophycus freshwateri - Neorhodomela aculeata, Caulacanthus okamurae - Sargassum thunbergii, Caulacanthus okamurae. Findings indicated that Sargassum thunbergii and Neorhodomela aculeata were the biomass-dominant species in the intertidal zone and Corallina officinalis and Undaria pinnatifida were biomass-dominant in the subtidal zone. Average values of marine algae biomass in the intertidal and subtidal zones were 252.6 g wet weight m^-2 and 291.9 g wet weight m^-2, respectively. Further, 38 new species were found in Beakryoung-do, with 25 of them specifically found in the subtidal zone. More studies on marine algal flora in the subtidal zone are needed to understand the changes in marine flora along the western coast.

• 한국식품과학회지
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• 제31권6호
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• pp.1667-1678
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• 1999

식품원재료 171종과 한약재 190종의 metanol 추출물의 항균활성을 조사하였다. 항균활성은 식품의 부패, 변질에 관여한다고 알려진 그람양성세균인 Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Listeria monocytogenes, 그람음성세균인 Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli 및 효모인 Saccharomyces cerevisiae, Candida albicans, 곰팡이 Penicillium citrinum, Aspergillus flavus, Aspergillus niger를 대상으로 측정하였다. 산수유, 오수유, 감초, 단삼, 오미자, 황련, 명일엽, aroma hop와 bitter hop는 그람양성세균에 항균활성을 나타내었다. Aroma hop와 bitter hop는 그람음성세균에 항균활성을 나타내었다. 한편, 그람양성세균에 대해 강한 항균활성을 나타낸 단삼으로부터 항균물질을 분리, 정제하고 그 구조를 결정하였다. 단삼의 methanol 추출액을 농축하여 ethylacetate로 extraction, silicagel column chromatography, preparative TLC, preparative HPLC 등을 통하여 보존활성물질(T-1)을 분리, 정제하고 UV, $^1H-NMR,\;^{13}C-NMR$, DEPT 및 EI-MS 등의 기기분석한 결과, 단삼의 항균활성물질은 cryptotanshinone으로 결정되었다. 그람양성균에 대하여 MIC는 $3.91{\sim}62.50\;{\mu}g/mL$ 범위에서 강한 보존활성을 나타내었고 특히 Bacillus subtilis에 대하여 MIC는 $3.91\;{\mu}g/mL$로 가장 강한 보존활성을 나타내었다. 따라서 단삼의 cryptotanshinone은 그람양성세균 및 그람음성세균에 의해 변패될 수 있는 식품에서 천연보존료로 이용 가능할 것으로 생각된다.

• 한국응용곤충학회지
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• 제45권3호
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• pp.347-355
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• 2006

약용식물 25종 중 지모, 창포, 쇠무릎, 짚신나물, 패랭이, 큰뱀무, 약모밀, 원추천인국, 아주까리, 현삼, 흑참깨, 기린초, 오이풀 등 13종의 식물체 추출액 5배와 10배의 희석농도에서 살선충 효과가 있는 것으로 나타났다. 더덕을 Pot에 파종하여 13종의 식물 추출액 5배액의 살선충 효과를 평가한 결과 짚신나물을 제외한 12종의 식물체 추출액에서 살선충 효과가 있었고, 지모, 쇠무릎, 창포, 패랭이, 원추천인국, 아주까리 등 6종의 추출액은 80% 이상의 억제효과를 나타내었다. 선발된 약용식물을 재배했었던 포장에 더덕을 파종하고 당근뿌리혹선충의 감염정도를 조사한 결과, 대조구에 비해 짚신나물을 제외한 지모 등 12종의 식물 포장에서 뿌리혹 및 토양 내 유충밀도가 적게 발생하였으며, 80% 이상의 방제가를 나타내었다. 선발된 약용식물을 퇴비처럼 처리한 포장에 더덕을 파종하였을 때는, 뿌리혹수 및 난낭수, 토양 내 유충밀도가 무처리에 비해서 흑참깨 처리를 제외하고는 적게 발생하였으나, 방제효과는 선발된 약용식물을 재배했던 포장에 비해 크게 떨어졌으며, 쇠무릎과 창포 처리의 방제가만 70% 이상으로 다른 식물에 비해 좋았다.

• 한국융합학회논문지
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• 제8권12호
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• pp.109-117
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• 2017

본 연구는 지리산 자생식물 19종을 선별하여 항산화 활성 및 항균 효과를 조사하여 천연보존제 융합소재 탐색을 목적으로 하였다. 총폴리페놀은 차나무(87.9 mg GAE/g), 박하(85.1 mg), 연(65.0 mg), 소나무(52.8 mg) 순으로 높은 값을 나타내었다. 총플라보노이드는 연(25.7 mg QUE/g)과 박하(25.4 mg QUE/g)에서 높은 함유량을 나타내었다. $100{\mu}g/mL$ 농도에서 차나무(58.1%), 연(47.9%), 박하(40.6%) 순으로 높은 ABTS radical 소거능을 나타냈으며, DPPH radical 소거능도 비슷한 경향을 나타내었다. 골잎원추리, 솔잎, 산수유 종자 추출물이 E. coli의 증식억제능이 가장 높았고, 할미꽃, 삼백초, 청미래덩굴 추출물은 S. aureus균의 증식을 완전히 억제하여 향후 천연보존 및 방부제 등의 융합소재로 개발가능성을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2405-2425
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• 2014

Radiation exposure leads to several pathophysiological conditions, including oxidative damage, inflammation and fibrosis, thereby affecting the survival of organisms. This review explores the radiation countermeasure properties of fourteen (14) plant extracts or plant-derived compounds against these cellular manifestations. It was aimed at evaluating the possible role of plants or its constituents in radiation countermeasure strategy. All the 14 plant extracts or compounds derived from it and considered in this review have shown some radioprotection in different in vivo, ex-vivo and or in vitro models of radiological injury. However, few have demonstrated advantages over the others. C. majus possessing antioxidant, anti-inflammatory and immunomodulatory effects appears to be promising in radioprotection. Its crude extracts as well as various alkaloids and flavonoids derived from it, have shown to enhance survival rate in irradiated mice. Similarly, curcumin with its antioxidant and the ability to ameliorate late effect of radiation exposure, combined with improvement in survival in experimental animal following irradiation, makes it another probable candidate against radiological injury. Furthermore, the extracts of P. hexandrum and P. kurroa in combine treatment regime, M. piperita, E. officinalis, A. sinensis, nutmeg, genistein and ginsan warrants further studies on their radioprotective potentials. However, one that has received a lot of attention is the dietary flaxseed. The scavenging ability against radiation-induced free radicals, prevention of radiation-induced lipid peroxidation, reduction in radiation cachexia, level of inflammatory cytokines and fibrosis, are some of the remarkable characteristics of flaxseed in animal models of radiation injury. While countering the harmful effects of radiation exposure, it has shown its ability to enhance survival rate in experimental animals. Further, flaxseed has been tested and found to be equally effective when administered before or after irradiation, and against low doses (${\leq}5Gy$) to the whole body or high doses (12-13.5 Gy) to the whole thorax. This is particularly relevant since apart from the possibility of using it in pre-conditioning regime in radiotherapy, it could also be used during nuclear plant leakage/accidents and radiological terrorism, which are not pre-determined scenarios. However, considering the infancy of the field of plant-based radioprotectors, all the above-mentioned plant extracts/plant-derived compounds deserves further stringent study in different models of radiation injury.

• 한국축산식품학회지
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• 제34권4호
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• pp.533-542
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• 2014

This study was performed to investigate the effects of lemon balm (Melissa officinalis L.) on various quality and antioxidant activity of hamburger patties. Lemon balm extract (LBE) showed the highest amount of total polyphenol (801.00 mg TAE/g DW) and flavonoids (65.05 mg RA/g DW). The $IC_{50}$ value of DPPH hydroxyl scavenging of LBE was $132{\mu}g/mL$. The hamburger patties were prepared by 0% (N), 0.1% (L1), 0.5% (L2), and 1.0% (L3) of the lemon balm powder. The addition of lemon balm powder increased the chewiness value, but did not affect the hardness, cohesiveness, and springiness values. Lemon balm powder had positive effects on sensory evaluation of patties. The pH of all patties decreased with longer storage period. 2-Thiobarbituric acid value, volatile basic nitrogen content, and the total microbial counts of hamburger patties in the L3 group were lower, compared to those of the normal (N group). In conclusion, the L3 group had significantly delayed lipid peroxidation compared to other treatment groups. However, the addition of lemon balm powder into patties showed no significantly influence on proximate composition, calorie contents, water holding capacity and cooking loss of patties. Therefore, lemon balm might be a useful natural antioxidant additive in meat products.

• 생약학회지
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• 제8권1호
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• pp.1-15
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• 1977

In an attempt to deduce which plants might have been used for their anticancer activities in traditional oriental herb medicine, 127 prescriptions were selected from 'Dong-Eui-Bo-Gam', the Classic Handbook of Korean Traditional Medicine, written by $H_{UH}$ Jun and published in 1613. These are the prescriptions indicated for the systemic treatment of various tumors and some conditions resembling tumors, e.g. inflammatory masses and indurations, and they include 150 natural products of plant origin. The frequency of appearance of each medicinal plant in these selected prescriptions was compared with the frequency of its appearance in all prescriptions listed in 'Bang-Yak-Hap-Pyon', another popular Oriental Medicine Formulary in Korea, written by $H_{WANG}\;Pil-Su$ in 1885. From the latter book, $H_{ONG}$ has recently enumerated frequencies of 235 medicinal plants included in a total of 467 prescriptions. Chi-square tests revealed that 11 plant remedies appear with significantly higher frequency in the prescriptions for "tumors", and 10 for "inflammations". The plants with potential antitumor activities, in decreasing order of statistical significance, are Scirpus maritimus, Curcuma zedoaria, Prunus persica, Rheum coreanum, Foeniculum vulgare, Rhus vernifera, Daphne pseudogenkwa, Galarhaeus sieboldiana, Croton tiglium, Raphanus sativus and Galarhaeus pekinensis. The drugs for potential antibacterial or anti-inflammatory activities are Olibanum(Frankincense), Forsythia coreana, Lonicera japonica, Gleditchia officinalis, $M_{YRRH}$, Trichosanhes kirilowii, Astragalus membranaceus, Rheum coreanum, Platycodon grandiflorum and Fritillaria verticillata. Despite the uncertainties involved in the terminology of various diseases used in pre-modern medicine, and the reservations about the efficacy of remedies used for those diseases, it would be worthwhile to investigate these few selected plants for anticancer, antibacterial, anti-inflammatory or antifungal effects, employing modern scientific methodology.

• 동의생리병리학회지
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• 제19권6호
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• pp.1541-1545
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• 2005

The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Corm fructus. The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being $200{\mu}g/mL$. The extract at concentrations of $10-500{\mu}g/ml$ showed dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (63.0%) was blocked by the extract (10, 50, 100, 250 and $500{\mu}g/ml$) concentration-dependently. Furthermore, the extract (50, 100 and $250{\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Corm fructus has potent antioxidant activity.

• 분석과학
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• 제18권3호
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• pp.188-193
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• 2005

천연 식물 추출물로 처리한 matrix metalloproteinase (MMP)의 저해능 실험에서 귀전우 (Euonymus alatus)의 methanol 가용성 분획이 matrix metalloproteinase (MMP-9) 활성에 저해능이 있는 것으로 나타났다. Silica gel column에서 ethyl acetate와 hexane의 혼합비를 달리하는 방법으로 다섯 개의 부분으로 분획하였고 그 중에서 세 개의 분획이 MMP-9 활성에 저해능이 있는 것으로 나타났다. 인체 간암 세포인 Hep3B 세포주와 인체 정상 세포인 Chang 세포주를 같은 조건으로 키운 후 형태학적 변화를 관찰한 결과, 귀전우의 메탄올 추출물로 처리한 세포에서만 핵의 주위에 검은 반점이 넓게 분포되어 있는 것이 관찰되었다. 인체 정상 세포주에 대한 독성 확인 실험에서 대부분의 메탄올 가용성 분획은 독성을 나타내지 않는 것으로 나타났다.