• Microbiology and Biotechnology Letters
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• v.16 no.4
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• pp.275-281
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• 1988

To develop cellulase and xylanase-producing strain by protoplast fusion, alkalophilic Bacillus sp. F204 and K17 were treated with NTG(N-methyl-N'-nitro-N-nitrosoguanidine) and isolated anti-biotics resistant strains of S20 (Km$^r$ , Cm$^r$) and G70 (Str$^r$). The frequency of protoplast formation was about 95% when cells of mid-log phase were treated with 200$\mu\textrm{g}$/ml Iysozyme at 37$^{\circ}C$ for 30-45 minutes. Under addition of 0.4-0.5M sodium succinate, 0.5% casamino acid, 1.5% polyvinylpyrrolidone, 25mM MgC1$_2$ and 50mM CaC1$_2$ to the regeneration medium, the regeneration frequency of Bacillus sp. F204 and K17 was 24.9% and 26.2%, respectively. The fusion frequency was 6.6$\times$10$^{-6}$ in the presence of 30% polyethylene glycol 6000 containing 50mM $Ca^{++}$ at 45$^{\circ}C$ for 5 minutes. Cellulase complex and xylanase activities of fusant were compared with parental strains.

• Fisheries and Aquatic Sciences
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• v.5 no.3
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• pp.200-205
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• 2002

Temperature-dependent pharmacokinetics of ciprofloxacin (CIP) was studied in the cultured olive flounders, Paralichthys olivaceus, and black rockfish, Sebastes schlegeli using high performance liquid chromatography (HPLC) originally developed for quinolone determination from livestock. Pharmacokinetics of CIP was apparently affected by ambient water temperature. In a two-compartment model for flounders after oral dosage of 20 mg/kg, $K_{01},\;at\;13^{\circ}C$ and $23^{\circ}C$ were 4.18 and 1.20/hr, respectively. The $K_{10},\;T_{max}\;and\;C_{max}\;at\;13^{\circ}C$ were 5.574/hr, l4.37${\mu}g/mL\;and\;3.15{\mu}g/mL,$ respectively. The corresponding values at $23^{\circ}C$ were l2.84/hr, 15.39${\mu}g/mL\;and\;6.38{\mu}g/mL$, respectively. The AUC, $T_{1/2} (\alpha)\;and\;T_{1/2}\;(\beta)$ were 278.23 ${\mu}g \cdot hr/mL$, 0.24hr and 47.02hr at $13^{\circ}C$ and 3l7.8l${\mu}g \cdot hr/mL$, 0.30 hrs and 60.78hrs at $23^{\circ}C$ for the flounder, respectively. Similar CIP pharmacokinetics were revealed in the black rockfish after oral dosage of 20 mg/kg under the two water temperature regimes. These pharmacokinetical results have some implication in the optimal usage of recently introduced antibacterials in the farmed fish, which were primarily adapted for poultry and mammalian species.

• Journal of fish pathology
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• v.15 no.2
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• pp.49-56
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• 2002

Effects of Temperature on the Pharmacokinetics of norfloxecin (NFX) were studied in the cultured carp,Cyprinus carpio, and cel, Anguilla japonica, using high performance liquid chromatography (HPLC) originally developed for quinolone determination in livesrocks. Pharmacokinetics of NFX was apparently affected by ambiem water temperature. In a two-compaament model for carp after oral dosage of 20 mg/K01 at $13^{\circ}C$ and $23^{\circ}C$ and 5.20/hr, respectively. In carp the $K_{\iota\nu}$, $T_{max}$and $C_{max}$ for carp at $13^{\circ}C$ were 13.30/hr, 17.44 ${\mu}g$/$m\ell$ and 7.00 ${\mu}g$/$m\ell$, respectively. The" correspoeding values at $23^{\circ}C$ were 3.93/hr, 15.40 ${\mu}g$/$m\ell$ and 9.44 ${\mu}g$/$m\ell$, respectively. The AUC and T were 355.66 ${\mu}g$ hr/$m\ell$, and 12.70 hr at $13^{\circ}C$ and 417,24 ${\mu}g$ hr/$m\ell$ and 13.86 hrs at $23^{\circ}C$, respectively. Similar trends were revealed in the NFX pharmacokinetics of eel kept under the two water temperature regimes aftee oral NFX dosage of 20 mg/kg. These pharmacokinetkal results have some implication in the optimal usage of recently introduced antibacterials in farmed fish, which were originally adapted for poultry and mammalian species.

• Microbiology and Biotechnology Letters
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• v.13 no.2
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• pp.103-107
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• 1985

A variety of plasmid curing agents such as sodium dodecyl sulfate, acriflavine, ethidium bromide, and mitomycin-C were used to cure Bacillus subtilis cells of streptomycin resistant factor. The drug susceptibility was increased by 0.1％ sodim dodecyl sulfate at stationary growth phase. The curing frequency was obtained highly at 4 $\mu\textrm{g}$/$m\ell$ of acriflavine, 10 $\mu\textrm{g}$/$m\ell$ of ethidium bromide, and 200 $\mu\textrm{g}$/$m\ell$ of mitomycin-C. respectively. Curing action occurred competitively for the streptomycin and terramycin resistant factors in B. subtilis.

• Horticultural Science & Technology
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• v.16 no.3
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• pp.374-376
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• 1998

Effects of traditional fumigation (methyl bromide; MB) and combined fumigation (methyl bromide/phosphide; $MB/PH_3$) on the disinfestation, and phytotoxicity and longevity of cut flowers (lily and rose) were investigated. Combined fumigation with low concentrations of $7/3g/m^3\;(MB/PH_3$) at $10^{\circ}C$ immediately after transportation from harvested place was found to be much more effective in disinfestation of aphid, mite, and thrips than fumigation with $MB\;48g/m^3$ only at $20^{\circ}C$ after simulated transportation for 2 days. Quality and longevity of cut flowers measured after fumigation were better in flowers treated by combined fumigation ($7/3g/m^3,\;MB/PH_3$) at $10^{\circ}C$ for 2.5-3 hrs, irrespective of pretreatment with pulsing solution immediately after transporation, even though phytotoxicity varied with cut flowers fumigated. On the other hand, fumigation with $MB\;48g/m^3$ only at $20^{\circ}C$ after simulated transportation for 2 days showed tendency of enhanced insect disinfestation, but caused phytotoxicity to both lily and rose, regardless of pretreatment and moreover, reduced the longevity of the cut flowers.

• Korean journal of food and cookery science
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• v.28 no.1
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• pp.25-32
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• 2012

This study was carried out to evaluate biological activities concerning extracts according to extraction methods from unripened fruit of Rubus coreanus Miq. The extraction methods were HWE (hot water extraction for 4 hr at $100^{\circ}C$), SFE (extraction for 3 hr at $40^{\circ}C$ under 300 bar, 100% of $CO_2$ fluid), USE (ultrasonification extraction for 4 hr at $50^{\circ}C$ with water), USE+HWE (hot water extraction for 2 hr at $100^{\circ}C$ after ultrasonification process for 2 hr), VE (vacuum extraction for 4 hr at $90^{\circ}C$ under 0.9 bar with water). VE extract showed the highest contents of total polyphenol ($178.78{\pm}3.79\;mg/g$) and total flavonoid ($40.93{\pm}0.68\;mg/g$). $IC_{50}$ values of DPPH radical scavenging activity, linoleic acid peroxidation inhibition activity and LDL (low density lipoprotein) oxidation inhibition activity of HWE extract showed the lowest $35.39{\pm}0.25{\mu}g/mL$, $12.61{\pm}0.31{\mu}g/mL$ and $1.31{\pm}0.02{\mu}g/mL$ among other all extracts, respectively. $IC_{50}$ values of ${\alpha}$-glucosidase inhibitory activities of VE and HWE extracts showed lower $14.34{\pm}0.20{\mu}g/mL$ and $15.83{\pm}0.20{\mu}g/mL$ than those of other extracts, respectively. Specifically, HWE and VE extracts have relatively better biological activities than other extracts; these could be potentially used as a bioactive source for health functional foods.

• Food Science and Preservation
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• v.17 no.2
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• pp.281-289
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• 2010

We determined the optimum ethanolic conditions for extraction of $\gamma$-oryzanol and other functional components from rice bran, using response surface methodology (RSM). A central composite design was used to investigate the effects of the independent variables of solvent ratio ($X_1$), extraction temperature ($X_2$), and extraction time ($X_3$), on dependent variables including yield ($Y_1$), total phenolic content ($Y_2$), electron-donating activity ($Y_3$), ferulic acid level ($Y_4$), and $\gamma$-oryzanol concentration ($Y_5$). Solvent ratio and extraction temperature were the most important factors in extraction. The maximum yield was at 22.56 mL/g ($X_1$), 78.19C ($X_2$), and 522.15 min ($X_3$), at the saddle point. Total phenolic levels were little affected by solvent ratio or extraction temperature. The maximum concentration of extracted total phenolics was 90.78mg GAE/100 g at 21.26 mL/g, $94.65^{\circ}C$, and 567.97 min. A maximum electron-donating ability of 54.72% was obtained with the parameters 20.20 mL/g,$81.89^{\circ}C$, and 701.87 min, at the highest point. The maximum level of ferulic acid components was 210.47 mg/100g at 5.22 mL/g, $79.66^{\circ}C$, and 575.24 min. In addition, the maximum $\gamma$-oryzanol concentration was 660.39 mg/100g at 5.10 mL/g, $81.83^{\circ}C$, and 587.39 min. The optimum extraction conditions were a solvent ratio of 10.45 mL/g, $80^{\circ}C$ extraction temperature, and 535 min extraction time. Predicted extraction levels under optimized conditions were in line with experimental values.

• Animal cells and systems
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• v.10 no.4
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• pp.191-196
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• 2006

This study was carried out to evaluate soil carbon cycling of a 36-year-old larch (Larix leptolepis) stand in Korea. The aboveground and soil organic carbon storage, litterfall, and soil respiration rates were measured over twoyear periods. The estimated aboveground biomass carbon storage and increment were 4220 gC $m^{-2}$ and 150 gC $m^{-2}\;yr^{-1}$, respectively. Mean organic carbon inputs by needle and total litterfall were 118 gC $m^{-2}\;yr^{-1}$ and 168 gC $m^{-2}\;yr^{-1}$, respectively. The aboveground carbon increment of the stand was similar to the annual input of carbon from total litterfall. The soil respiration rates correlated exponentially with the soil temperature at a depth of 20 cm ($R^2$ = 0.86). In addition, the exponential regression equation indicated a relatively strong positive relationship between the soil respiration rates and soil temperature, while there was no significant relationship between the soil respiration rates and the soil moisture content. The annual mean and total soil respiration rates were 0.40 g $CO_2\;m^{-2} h^{-1}$ and 3010 g $CO_2\;m^{-2}\;yr^{-1}$ over the two-year study period, respectively.

• Journal of Wetlands Research
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• v.18 no.2
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• pp.201-207
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• 2016

In LID facilities treating stormwater runoff, various kinds of plants are applied for water circulation recovery and pollutant reduction. However, rapid changes of soil moisture due to the use of porous media and spread of deicing material during winter season cause slow plant growth that detrimentally leads to many problems including death of plants. Therefore, this study was performed to evaluate the salt resistance of plants, its effects on pollutants removal, and water circulation recovery. Eight different kinds of plants applicable to an LID facility were selected for the experiment, which were Bridal wreath (Spiraea japonica, S.J), Azalea (Rhododendron indicum, R.I), Dawn Redwood (Metasequoia glyptostroboides, M.G), Sweet flag (Acorus calamus A.C), Dwarf fan-shape columbine(Aquilegia flabellata, A.F), Pink (Dianthus chinensis, D.C), Pratia pedunculata (Pratia pedunculata, P.B) and Marigold (Tagetes patula, T.P). Woody plants such as S.P, R.I, and M.G appear to have less salt resistance compared to the other herbaceous plants. Specifically, M.G achieved the highest salt resistance among the other woody plants being followed by S.P, and R.I, respectively. For herbaceous plants, T.L and D.C have the higher salt resistances than that of A.C, P.B, and A.F, respectively. Regardless of the influence of salt to most of the plants, TN and TP were reduced more than 60% and the study suggests the M.G showed high pollutant removal efficiency and provided better water circulation by means of active photosynthesis and respiration due to higher growth.

• Nuclear Engineering and Technology
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• v.6 no.2
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• pp.89-96
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• 1974

The cross-section ratios of the nuclear isomeric pairs $^{80}$ B $r^{m, g}$, sup 81/S $e^{m, g}$, $^{104}$ R $h^{m, g}$, $^{116}$ I $n^{m, g}$ and $^{134}$ C $s^{m, g}$ through the radiative thermal neutron capture process have been studied. The experimental values of these ratios obtained by the activation method have been compared with the calculated ones deduced from the modified Huizenga-Vandenbosch method. Agreement between these values within 30% could be attained by controlling the spin cut-off parameter and gamma-ray multiplicity.