• 제목/요약/키워드: M/G/1/K

검색결과 14,557건 처리시간 0.043초

M.9 계통 사과 대목의 기내 급속 번식 (Rapid Multiplication of M.9 Apple Rootstocks in vitro)

  • 전지혜;정경호;정상복;홍경희;강상조
    • 원예과학기술지
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    • 제19권1호
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    • pp.34-38
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    • 2001
  • 사과 M.9 자근대목의 효과적인 기내 급속 대량 번식법을 구명하기 위하여 본 연구를 수행한 결과, 신초 증식에 적합한 배지는 $1.0mg{\cdot}L^{-1}$ BA, $0.1mg{\cdot}L^{-1}$ IAA, $30g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 MS 배지이며, 치상방법은 신초 정단부를 제거한 후 수평치상하는 것이 효과적이었다. 발근에 적합한 배지는 $0.5mg{\cdot}L^{-1}$ IBA, $20g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 1/2 MS 배지였다.

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모시잎의 항산화 효과 및 암세포주에 대한 세포 독성 (Antioxidant Activities and Cell Viability against Cancer Cells of Adenophora remotiflora Leaves)

  • 김인숙;박권삼;유현희;신미경
    • 동아시아식생활학회지
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    • 제19권3호
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    • pp.384-394
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    • 2009
  • 본 연구는 모시잎의 핵산 분획물, 에틸아세테이트 분획물, 메탄올 분획물, 물 추출물에 대해 항산화성 효과와 BNLcl2 cell(인체 정상 간세포)의 세포독성 실험, A549 cell(폐암세포 주)와 Hep G2 cell(간암세포주)에 대한 항암성을 탐색한 결과는 다음과 같다. 모시잎의 각 용매별 분획물 시료를 DPPHradical 소거능으로 항산화 효과를 검토한 결과, $SC_{50}$이 메탄올 분획물, 물 추출물, BHT가 각각 47.5 ${\mu}g$/mL, 74.6 ${\mu}g$/mL, 102.2 ${\mu}g$/mL로 나타나, 이들 모시잎 분획물은 합성항산화제인 BHT보다 항산화 활성이 높음을 알 수 있었다. 또한, 헤모 글로빈 유도 linoleic acid의 산화 억제 활성을 측정한 결과에서도 메탄올 분획물과 물 추출물에서 $IC_{50}$이 메탄올 분획물, 물 분획물 120.8 ${\mu}g$/mL, 135.6 ${\mu}g$/mL로 BHT의 150.2 ${\mu}g$/mL보다 낮아 항산화 효과가 높았다. BNLcl2 cell(인체 정상 간세포)에서 대조군에 대한 모시잎의 용매 분획별, 농도별(1, 5, 10, 25, 50, 100, 250, 500, 1,000, 2,000 ${\mu}g$/mL)로 처리한 실험군의 세포 생존율의 저하를 알아본 결과, 물 분획물은 모든 농도에서 대조군에 비해 유의적인 생존율의 저하는 없었으나, 핵산, 에틸아세테이트, 메탄올 분획물은 1,000 ${\mu}g$/mL 이상에서 대조군에 비해 유의적(p<0.01~p<0.001)인 생존율의 저하가 있어 세포독성이 있는 것으로 나타났다. 항암 활성은 A549 cell 폐암세포주에 대해 메탄올 분획물이 다른 분획물에 비해 폐암세포주 생존율을 억제시켜 500 ${\mu}g$/mL에서 대조군보다 생존율이 46%까지 낮아졌다. Hep G2 cell 간암 세포주에 대한 항암 활성이 A549 cell보다 높았으며, Hep G2 cell에 대한 항암 활성은 500 ${\mu}g$/mL에서 메탄올 분획물은 28%까지 생졸이 낮았으며, 에틸아세테이트 분획물 30%, 물 추출물 36%, 핵산 분획물 52% 순이었다. 따라서 본 실험의 결과에 비추어 볼 때 모시잎은 산화와 변질의 원인이 되는 항산화 효과뿐만 아니라 폐암과 간암 세포의 성장을 저해하는 효과가 있었다. 총 폴리페놀 함량을 측정한 결과, 메탄올 분획물과 물 분획물에서 함량이 높아 항산화 활성과 폐암 세포의 성장 저해 물질은 폴리페놀 계통일 것으로 생각되며, 간암 세포 성장 저해는 전체 유기 용매 분획물에 효과가 나타난 것으로 보아 폴리페놀 계통 외에 다른 생리활성 물질이 있을 것으로 보여, 이들 물질에 대한 추후 연구가 요구되며, 앞으로 식품뿐만 아니라 의약품의 원료 및 첨가물 등 다양한 제품에 이용할 수 있을 것으로 보인다.

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In vivo Angiogenic Activity of Dichloromethane Extracts of Aloe vera Gel

  • Lee, Myoung-Jin;Yoon, Soo-Hong;Lee, Seung-Ki;Chung, Myung-Hee;Park, Young-In;Sung, Chung-Ki;Choi, Jae-Sue;Kim, Kyu-Won
    • Archives of Pharmacal Research
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    • 제18권5호
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    • pp.332-335
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    • 1995
  • The angiogenic activity of Aloe vera (Aloe baradensis), known as a good healing plant, was investigated. We have extracted and fractionated dichloromethane extract (G1M1D1) and methanol soluble fraction of dichloromethane extract (G1M1D1M1) which contain low-molecular weight substances of Aloe vera gel. G1M1D1 and G1D1M1 fractions induced a radially arranged, spoke-sheel-like vasculature in chick embryo chorioallantoic membrane (CAM) assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay. The angiogenic activity was dose-dependent and the angiogenic pattern in the CAM assay was very similar to that of phorbol 12-myristate-13-acetate (PMA) used as a positive control. The modified CAM assay, e simple and accurate quantitating method, was used to quntitate the angiogenic activity of G1D1M1 fraction. Application of G1M1D1M1 fraction ($100\mug/egg$) resulted in much more intense angiogenesis than in control while slightly less intense angiogenesis than in PMA (100 ng/egg).

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간척지 토양개량을 위한 내염성 식물의 활용성 평가 (Evaluation of Salt-Tolerance Plant for Improving Saline Soil of Reclaimed Land)

  • 이경보;강종국;;이덕배;박찬원;김재덕
    • 한국토양비료학회지
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    • 제40권3호
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    • pp.173-180
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    • 2007
  • 본 연구는 간척지 친환경농업 기반조성을 위하여 신간척지 토양개량을 위한 내염성 식물 활용성을 평가하고자 국내외에서 내염성 식물 18종을 수집하여 시험을 수행하였다. 신간척지 토양에서 파종된 식물의 출현율은 FL478(벼)>Barnyard grass>Pokkali>Atriplex>Sesbania>Rumex>Alfalfa>Tall Fescue>Ryegrass>Sudan grass의 순이었다. 신간척지 토양에서 생육후기까지 생육이 가능한 식물은 Limonium, Atriplex, Barnyard grass 및 Sesbania 등 4종이었다. Barnyard grass의 생체중은 $1.21kg\;m^{-2}$, 건물중은 $0.48kg\;m^{-2}$이었다. Sesbania의 생체중은 $1.55kg\;m^{-2}$, 건물중은 $0.68kg\;m^{-2}$로 가장 많았으며, Atriplex의 생체중은 $1.04kg\;m^{-2}$, 건물중은 $0.42kg\;m^{-2}$이었다. 그리고 Limonium의 생체중은 $1.04kg\;m^{-2}$, 건물중은 $0.32kg\;m^{-2}$이었다. Sesbania와 Barnyard grass를 시용한 결과 인산함량은 시험전 $62.5g\;kg^{-1}$에 비하여 각각 $76.1g\;kg^{-1}$, $78.1g\;kg^{-1}$으로 증가하였다. 유기물함량은 시험 전 토양이 $2.65g\;kg^{-1}$이었지만 내염성 식물을 시용함으로서 $2.81{\sim}4.60g\;kg^{-1}$으로 증가하였다. 녹비 시용 전 토양 용적밀도는 $1.42Mg\;m^{-3}$이었으나 Sesbania와 Barnyard grass를 시용한 토양의 용적밀도는 $1.39Mg\;m^{-3}$로 낮아졌다.

Effects of Microbacterium laevaniformans Levans Molecular Weight on Cytotoxicity

  • Oh, Im-Kyung;Yoo, Sang-Ho;Bae, In-Young;Cha, Jae-Ho;Lee, Hyeon-Gyu
    • Journal of Microbiology and Biotechnology
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    • 제14권5호
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    • pp.985-990
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    • 2004
  • Levans produced from Microbacterium laevaniformans were isolated, characterized, and fractionated by molecular weight. TLC, HPLC, and GC-MS analyses of the exopolysaccharide showed that it was a fructan-type polymer and was composed of (2,6)- and (2,1)-glycosidic linkages. $^{13}C$-NMR analysis proved that the polysaccharide was mainly a $\beta$-(2,6)-linked levan-type polysaccharide. To investigate the cytotoxicity of the acetone-precipitated levan fractions such as M1, M2, and M3, HepG2, P388D1, U937, SNU-1, and SNUC2A cell lines were screened. Among the cell lines tested, the cytotoxicity of M1- M3 fractions were detected from only SNU-1 and HepG2 cells at the dosage level of $100-800\mu\textrm{g}ml$. The M2 fraction M_r$, 80,000) at 400 $mu{g/ml}$ had the greatest cell growth inhibition (84.6%) on SNU-1, while the M1 $(M_r$, 50,000) at $800\mu\textrm{g}ml$ showed the greatest (46.32%) on HepG2. To obtain more uniform M_r$ fractions of levan, the levan was further fractionated from S1 $(M_r$ 1,000,000) to S5 $(M_r$ 10,000) using gel permeation chromatography. Again, the S1-S5 fractions had strong cytotoxicity on SNU-1 and HepG2 cell lines. The greatest inhibition effects of S4 $(M_r$ 80,000) on SNU-1 and S5 $(M_r$ 10,000) on HepG2 were shown to be 49.5% and 73.0%, respectively. The cytotoxicity of the levan fractions was more effective on SNU-1 than on HepG2. Although the relationship between the Mw and the cytotoxicity was not clear, smaller $M_r$, fractions of levan showed greater growth inhibition effect on the cancer cell lines in general. Therefore, it was indicated that a specific Mw class of levan is responsible for the effective cytotoxicity.

Soil Carbon Cycling and Soil CO2 Efflux in a Red Pine (Pinus densiflora) Stand

  • Kim, Choon-Sig
    • Journal of Ecology and Environment
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    • 제29권1호
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    • pp.23-27
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    • 2006
  • This study was conducted to evaluate forest carbon cycling and soil $CO_2$ efflux rates in a 42-year-old pine (Pinus densiflora) stand located in Hamyang-gun, Korea. Aboveground and soil organic carbon storage, litterfall, litter decomposition, and soil $CO_2$ efflux rates were measured for one year. Estimated aboveground biomass carbon storage and increment in this stand were $3,250gC/m^2\;and\;156gC\;m^{-2}yr^{-1}$, respectively. Soil organic carbon storage at the depth of 30 cm was $10,260gC/m^2$ Mean organic carbon inputs by needle and total litterfall were $176gC\;m^{-2}yr^{-1}\;and\;235gC\;m^{-2}yr^{-1}$, respectively. Litter decomposition rates were faster in nne roots less than 2 mm diameter size ($<220\;g\;kg^{-1}yr^{-1}$) than in needle litter ($<120\;g\;kg^{-1}yr^{-1}$). Annual mean and total soil respiration rates were $0.37g\;CO_2m^{-2}h^{-1}$ and $2,732g\;CO_2m^{-2}yr^{-1}$ during the study period. A strong positive relationship existed between soil $CO_2$ efflux and soil temperature (r=0.8149), while soil $CO_2$ efflux responded negatively to soil pH (r=-0.3582).

Anti-Melanogenic Effect of Oenothera laciniata Methanol Extract in Melan-a Cells

  • Kim, Su Eun;Lee, Chae Myoung;Kim, Young Chul
    • Toxicological Research
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    • 제33권1호
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    • pp.55-62
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    • 2017
  • We evaluated the antioxidant activity and anti-melanogenic effects of Oenothera laciniata methanol extract (OLME) in vitro by using melan-a cells. The total polyphenol and flavonoid content of OLME was 66.3 and 19.0 mg/g, respectively. The electron-donating ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity, and superoxide dismutase (SOD)-like activity of OLME ($500{\mu}g/mL$) were 94.5%, 95.6%, and 63.6%, respectively. OLME and arbutin treatment at $50{\mu}g/mL$ significantly decreased melanin content by 35.5% and 14.2%, respectively, compared to control (p < 0.05). OLME and arbutin treatment at $50{\mu}g/mL$ significantly inhibited intra-cellular tyrosinase activity by 22.6% and 12.6%, respectively, compared to control (p < 0.05). OLME ($50{\mu}g/mL$) significantly decreased tyrosinase, tyrosinase-related protein-1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor-M (MITF-M) mRNA expression by 57.1%, 67.3%, 99.0%, and 77.0%, respectively, compared to control (p < 0.05). Arbutin ($50{\mu}g/mL$) significantly decreased tyrosinase, TRP-1, and TRP-2 mRNA expression by 24.2%, 42.9%, and 48.5%, respectively, compared to control (p < 0.05). However, arbutin ($50{\mu}g/mL$) did not affect MITF-M mRNA expression. Taken together, OLME showed a good antioxidant activity and anti-melanogenic effect in melan-a cells that was superior to that of arbutin, a well-known skin-whitening agent. The potential mechanism underlying the anti-melanogenic effect of OLME was inhibition of tyrosinase activity and down-regulation of tyrosinase, TRP-1, TRP-2, and MITF-M mRNA expression.

Entire Functions and Their Derivatives Share Two Finite Sets

  • Meng, Chao;Hu, Pei-Chu
    • Kyungpook Mathematical Journal
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    • 제49권3호
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    • pp.473-481
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    • 2009
  • In this paper, we study the uniqueness of entire functions and prove the following theorem. Let n(${\geq}$ 5), k be positive integers, and let $S_1$ = {z : $z^n$ = 1}, $S_2$ = {$a_1$, $a_2$, ${\cdots}$, $a_m$}, where $a_1$, $a_2$, ${\cdots}$, $a_m$ are distinct nonzero constants. If two non-constant entire functions f and g satisfy $E_f(S_1,2)$ = $E_g(S_1,2)$ and $E_{f^{(k)}}(S_2,{\infty})$ = $E_{g^{(k)}}(S_2,{\infty})$, then one of the following cases must occur: (1) f = tg, {$a_1$, $a_2$, ${\cdots}$, $a_m$} = t{$a_1$, $a_2$, ${\cdots}$, $a_m$}, where t is a constant satisfying $t^n$ = 1; (2) f(z) = $de^{cz}$, g(z) = $\frac{t}{d}e^{-cz}$, {$a_1$, $a_2$, ${\cdots}$, $a_m$} = $(-1)^kc^{2k}t\{\frac{1}{a_1},{\cdots},\frac{1}{a_m}\}$, where t, c, d are nonzero constants and $t^n$ = 1. The results in this paper improve the result given by Fang (M.L. Fang, Entire functions and their derivatives share two finite sets, Bull. Malaysian Math. Sc. Soc. 24(2001), 7-16).

고객수 상태에 따른 서비스를 제공하는 M/G/1/K 대기체계에 관한 소고 (A Note on the M/G/1/K Queue with Two-Threshold Hysteresis Strategy of Service Intensity Switching)

  • 최두일;김보근;이두호
    • 한국경영과학회지
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    • 제39권3호
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    • pp.1-5
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    • 2014
  • We study the paper Zhernovyi and Zhernovyi [Zhernovyi, K.Y. and Y.V. Zhernovyi, "An $M^{\Theta}/G/1/m$ system with two-threshold hysteresis strategy of service intensity switching," Journal of Communications and Electronics, Vol.12, No.2(2012), pp.127-140]. In the paper, authors used the Korolyuk potential method to obtain the stationary queue length distribution. Instead, our note makes an attempt to apply the most frequently used methods : the embedded Markov chain and the supplementary variable method. We derive the queue length distribution at a customer's departure epoch and then at an arbitrary epoch.

개 외이도에서 분리한 세균 및 M pachydermatis의 약제감수성에 대하여 (Drug susceptibility of bacteria and M pachydermatis isolated from canine external ear canals)

  • 김기향;최원필;여상건
    • 대한수의학회지
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    • 제39권3호
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    • pp.560-565
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    • 1999
  • The present work was conducted to investigate the drug susceptibility of microorganisms isolated from canine external ear canals. Antifungal susceptibility test of M pachydermatis (17 strains) was perfomed by agar dilution method, using 11 antifungal drugs including amphotericin B(A), nystatin(N), pimaricin(P), griseofulvin(G), bifonazole(B), clotrimazole(C), miconazole(M), econazole(E), ketoconazole(K), tolnaftate(T), 5-fluorocytosine(F). All isolates were highly sensitive to K, M, T(geometric mean MIC ; GM $MIC{\leq}0.16{\mu}g/ml$) but they weren't sensitive to P, F and G(GM $MIC{\geq}92.37{\mu}g/ml{\sim}{\geq}128{\mu}g/ml$). Antibacterial susceptibility test against 119 isolates of bacteria was performed by agar dilution method, using 9 antibacterial drugs including erythromycin(ET), chloramphenicol(CP), gentamycin(G), vancomycin(V), ampicillin(AP), amoxacillin(AX), chlortetracycline(CT), ciprofloxacin(CF), enrofloxacin(EF). All isolates of Staphylococcus spp(101 strains) were highly sensitive to EF, CF, G(GM MIC $0.33{\sim}1.47{\mu}g/ml$). In other gram positive cocci(4 strains), they were highly sensitive to EF, CF, V(GM MIC $1{\sim}4.76{\mu}g/ml$) and CT(GM MIC 1 UFL unit/ml). In gram positive rods(13 strains), they were highly sensitive to EF, CF, G(GM $MIC{\leq}0.19{\sim}1{\mu}g/ml$). In Pseudomonas aeruginosa(1 strain), it was highly sensitive to AX, EF, ET, CF(GM MIC $0.06{\sim}1{\mu}g/ml$) and CT(GM MIC 1 UFL unit/ml). All isolates weren't sensitive to AP(GM MIC 16~>$32{\mu}g/ml$).

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