Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Rapid Multiplication of M.9 Apple Rootstocks in vitro

M.9 계통 사과 대목의 기내 급속 번식

  • Jun, Ji Hae (Fruit Tree Breeding Division, National Horticultural Research Institute, RDA) ;
  • Chung, Kyeong Ho (Fruit Tree Breeding Division, National Horticultural Research Institute, RDA) ;
  • Jeong, Sang Bouk (Fruit Tree Breeding Division, National Horticultural Research Institute, RDA) ;
  • Hong, Kyung Hy (Naju Pear Research Institute, National Horticultural Research Institute, RDA) ;
  • Kang, Sang Jo (Research Management Bureau, RDA)
  • Received : 2000.12.13
  • Accepted : 2000.12.27
  • Published : 2001.03.31
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Abstract

This experiment was conducted to find out the optimum cultural conditions for the propagation of M.9 apple rootstocks such as EMLA M.9 and NAKB T-337 in vitro. Murashige and Skoog (MS) medium supplemented with $1.0mg{\cdot}L^{-1}$ BA, $0.1mg{\cdot}L^{-1}$ IAA, $30g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ agar was suitable for shoot proliferation. Removing of apical meristem and horizontal placing of explants on medium increased shoot proliferation significantly. The best rooting was obtained on 1/2 MS medium supplemented with 0$0.5mg{\cdot}L^{-1}$ IBA, $20g{\cdot}L^{-1}$ sucrose, and $8g{\cdot}L^{-1}$ agar.

사과 M.9 자근대목의 효과적인 기내 급속 대량 번식법을 구명하기 위하여 본 연구를 수행한 결과, 신초 증식에 적합한 배지는 $1.0mg{\cdot}L^{-1}$ BA, $0.1mg{\cdot}L^{-1}$ IAA, $30g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 MS 배지이며, 치상방법은 신초 정단부를 제거한 후 수평치상하는 것이 효과적이었다. 발근에 적합한 배지는 $0.5mg{\cdot}L^{-1}$ IBA, $20g{\cdot}L^{-1}$ sucrose, $8g{\cdot}L^{-1}$ agar가 첨가된 1/2 MS 배지였다.

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