• Korean Journal of Microbiology
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• v.30 no.6
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• pp.514-518
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• 1992

The present study has been perromed to investigate the optimal conditions for protoplast formation and regeneration of oleandomycin-producing Streptomyces antibioticus (S. antibioticus) KCTC 1081. Mycelia were grown in YME medium containing 0.2% (w/v) glycine and converted into the protoplast by incubating at 35.deg.C for 60 minutes in protoplast buffer (P buffer) containing 4 mg/ml lysozyme. The reversion of protoplasts to the normal filamentous state was examined by the growth on various synthetic agar media. A high reversion rate was obtained by incubating the protoplasts on a hypertonic agar medium containing 20 mM $Mg^{++}$, 5 mM $Ca^{++}$ and 0.3 M sucrose at 28.deg.C for 5 days. From these experiments, we established the improved regeneration medium and a protocol which supports higher and more consistent levels of regeneration of S. antibioticus protoplasts. The regenerant showed an increased antimicrobial activity compared with that of the initial strain.n.n.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.45 no.10
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• pp.59-65
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• 2008

We have proposed a UHF/HF multi-band RFID reader, and have implemented it into a system in a package(SiP). The proposed SiP RFID reader has been designed to support both for EPCgloabal Class1 Generation2 protocol of UHF band, and 13.56MHz RFID protocols of ISO14443 A/B type, and ISO15693 standards. The operating mode is controlled by embedded RISC core, and the mode can be selected by users. The area of implemented SiP is $40mm{\times}40mm$ with 4 metal layers. The implemented reader SiP operates at single supply voltage of 3.3V. The maximum current consumption is 210mA. The operating distances are 5cm for 13.56MHz modes, and 20cm for UHF mode.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.8
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• pp.964-969
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• 2007

A regulated low phase noise differential colpitts VCO(Voltage Controlled Oscillator) for mobile RFID system is presented. The differential colpitts VCO meets the dense reader environment specifications. The VCO use a $0.35{\mu}m$ technology and achieves tuning range $1.55{sim}2.053 GHz$. Measuring 910 MHz frequency divider output, phase noise performance is -106 dBcMz and -135dBc/Hz at 40 kHz and 1MHz offset, respectively. 5-bit digital coarse-tuning and accumulation type MOS varactors allow for 28.2% tuning range, which is required to cover the LO frequency range of a UHF Mobile RFID system, Optimum design techniques ensure low VCO gain(<45 MHz/V) for good interoperability with the frequency synthesizer. To the author' knowledge, this differential colpitts VCO achieves a figure of merit(FOM) of 1.93dB at 2-GHz band.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.2
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• pp.147-152
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• 2009

In this study, the applicability of alginate-immobilized Pseudomonas putida mt-2 KG1206 on the environments, contaminated with toluene analogs was conducted. Genetically engineered strain KG1206 produces light by direct (m-toluate, benzoate) and indirect (toluene, xylenes) inducers. The protocol for the alginate-immobilization was determined in terms of the cell to alginate ratio, solution, proper number of alginate beads, and other conditions. Maximum bioluminescences of five chemicals by immobilized strain were generally observed in following orders: m-toluate > p-xylene > toluene > o-xylene > m-xylene. In relationship between bioluminescence activity and inducer reduction, initial m-toluate (5 mM) in solution was removed approximately 48% of initial at 5 h exposure, showing continuous decrease of inducer chemical in solution. These results of study with alginate-immobilized beads would be useful, especially, for biomonitoring of contaminated environments with specific compounds, such as petroleum hydrocarbon compounds including toluene analogs.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.421-424
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• 2013

차량 애드 혹 네트워크는 도심 교차로에서 도로의 특성과 차량의 높은 이동성으로 인해 네트워크의 단절이 자주 일어난다. 향상된 지리기반 라우팅 프로토콜인 GBSR(Greedy Border Superiority Routing)은 V2V(Vehicle-to-Vehicle)에 적합하다. 그러나 탐욕모드에서 stale노드가 로컬최대에 직면하는 문제를 가지고 있다. EGBSR(Enhanced GBSR)은 반대 방향의 차량과 같은 방향의 차량에게 보내는 비콘 메시지와 도로 정보를 관리하는 테이블을 이용하여 경로를 탐색하고 설정된 경로를 통해 패킷을 보낸다. 이를 통해 소스노드가 목적지노드에 빠르고 안전하게 패킷을 전달할 수 있는 양방향 라우팅 기법 제안한다.

• Proceedings of the Korea Information Processing Society Conference
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• 2024.05a
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• pp.149-150
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• 2024

차량 애드 혹 네트워크(VANET)는 운전자에게 안전하고 편안한 운전을 위한 다양한 서비스를 제공한다. VANET 은 차량의 이동으로 인해 토폴로지 변화가 빠르게 발생하기 때문에 무선 링크 품질과 링크 안정성의 변화가 극심하다는 문제가 있다. 본 논문에서는 VANET 을 위한 새로운 라우팅 프로토콜을 제안한다. 제안하는 프로토콜은 링크 품질과 링크 안정성을 사용하여 최적의 라우팅 경로를 제공하며, 네트워크 QoS 를 제공할 수 있다. 시뮬레이션 결과는 처리량, 패킷 손실률, 그리고 종단 간 지연 측면에서 상당한 성능 개선을 보여준다.

• ETRI Journal
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• v.37 no.2
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• pp.233-240
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• 2015

The novelty of this study resides in a 6"-wafer-level microfabrication protocol for a microdevice with a fluidic control system for the separation of circulating tumor cells (CTCs) from human whole blood cells. The microdevice utilizes a lateral magnetophoresis method based on immunomagnetic nanobeads with anti-epithelial cell adhesive molecule antibodies that selectively bind to epithelial cancer cells. The device consists of a top polydimethylsiloxane substrate for microfluidic control and a bottom substrate for lateral magnetophoretic force generation with embedded v-shaped soft magnetic microwires. The microdevice can isolate about 93% of the spiked cancer cells (MCF-7, a breast cancer cell line) at a flow rate of 40/100 mL/min with respect to a whole human blood/buffer solution. For all isolation, it takes only 10 min to process 400 mL of whole human blood. The fabrication method is sufficiently simple and easy, allowing the microdevice to be a mass-producible clinical tool for cancer diagnosis, prognosis, and personalized medicine.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.5
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• pp.963-970
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• 2008

Recently, several papers for reading meters remotely using RFID/USN technologies have been presented. In the case of water meter, there has been neither commercial product nor paper. In this paper, we describe the design and implementation of wireless digital water meter with low power consumption. We use magnetic hole sensors to compute the amount of water consumption. The meter of water consumption is transferred via ZigBee wireless protocol to a gateway. Low power consumption design is essential since a battery should last till the life time of water meter. We present that dual batteries haying 3V, 3000mAh, would last 8 years by analyzing the real power consumption of our water meter.

• Archives of Pharmacal Research
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• v.28 no.4
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• pp.463-468
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• 2005

The purpose of the present study was to develop a standard protocol for imidapril hydrochloride bioequivalence testing. For this reason, a specific LC-MS method was developed and validated for the determination of imidapril in human plasma. A solid-phase extraction cartridge, $Sep-pak^{R}$ C18, was used to extract imidapril and ramipril (an internal standard) from deproteinized plasma. The compounds were separated using a XTerra $MS^{R}$?C18 column ($3.5 {\mu}m, 2.1\times150 mm$) and $acetonitrile-0.1\%$ formic acid (67:33, v/v) adjusted to pH 2.4 by 2 mmol/L ammonium formic acid, as mobile phase at 0.3 mL/min. Imidapril was detected as m/z 406 at a retention time of ca. 2.3 min, and ramipril as m/z 417 at ca. 3.6 min. The described method showed acceptable specificity, linearity from 0.5 to 100 ng/mL, precision (expressed as a relative standard deviation of less than $15\%$), accuracy, and stability. The plasma concentration-versus-time curves of eight healthy male volunteers administered a single dose of imidapril (10 mg), gave an $AUC_{12hr}$ of imidapril of $121.48\pm35.81 ng mL^{-1} h$, and $C_{max} and T_{max}$ values of $32.59\pm9.76 ng/mL and 1.75\pm0.27 h$. The developed method should be useful for the determination of imidapril in plasma with sufficient sensitivity and specificity in bioequivalence study.

• Fisheries and Aquatic Sciences
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• v.25 no.8
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• pp.429-440
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• 2022

This study assessed the effects of oxygen and ozone nanobubbles on gill morphology, weight gain, and mortality of Pacific white shrimp (Penaeus vannamei), as well as the level of Vibrio parahaemolyticus and water quality of shrimp culture tanks under lab conditions. Two experiments were carried out with oxygen macrobubble, ozone macrobubble, oxygen nanobubble, ozone nanobubble, and control treatments (air-stone macrobubble). Experiments were done in triplicate in 100 L tanks with 15‰ saline water, and 20 shrimp per tank. Tanks in Experiment 1 were not inoculated with bacteria; tanks in Experiment 2 were inoculated with V. parahaemolyticus at a concentration of 10⁶ CFU/mL. The results revealed that short treatments with ozone nanobubbles had minimal impact on shrimp gills, mortality, and growth rates, reduced V. parahaemolyticus concentration in water compared to the other groups, and improve water quality. These laboratory results indicate that ozone nanobubble treatment may be useful for controlling V. parahaemolyticus. More work is needed to find the best protocol to apply the technology on a commercial scale.