• Korean Journal of Veterinary Research
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• v.2 no.2
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• pp.29-31
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• 1962

Although Lycaconitine, one of the alkaloids of Radix Jingyu, had been identified in 1884, its use in practice has not been known probably because of our limited knowledge on pharmacologic actions of the drug. Recently repellent efficacy of Lycaconitine against Korean cattle ticks and goat ticks was demonstrated. Obviously, there in a need to investigate toxicologic aspects of Lycaconitine before its widespread use for the control of animal ticks can be attempted. As will be reported in this paper, Lycaconitine Shows a wide margin of safety in the concentration applied as cattle or goat tick repellent.

• Korean Journal of Veterinary Research
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• v.2 no.2
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• pp.15-24
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• 1962

In the previous report it was demonstrated that aqueous extracts of two Korean indigenous crude drugs, Radix Jingyu and Fructus ponciri, have an excellent tick repellent effect, and also the need for further analytical study of these crude drugs was suggested. In the experiments to be reported herein, attempts were made to find out the active ingredients from Radix Jingyu, since the aqueons extract of Radix Jingyu proved to be mone effective than that of Fructus ponciri. It will be shown that the active ingredient is indicative of Lycaconitine, a known alkaloid. An aqueons solution containing 1.5 Gms of Lycaconitine in tartrate when sprayed on a cow exerted tick repellent effect against Boophilus microplus which lasted approximately 25 days without any harmful influences on cattle. It will also be shown that the oil-fat components of Radix Jingyu act synergistically with Lycaconitine in accelerating and lengthening the effect, although the components alone have no effect under the conditions tested. It was of interest to note that the oil-fat components of Radix Jingyu showed definite repellent effect against the larvae of Boophilus microplus in laboratory experiments by the method newly devised. On the basis of these observations, it was concluded that Lycaconitine can be used as cattle tick repellent, and the evidence seems to warrant a large scale production of Lycaconitine for its widespread use. Further studies on the possible effect of the alkaloid on other genera or species of ticks and related vermins were also indicated.

• Korean Journal of Veterinary Research
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• v.2 no.2
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• pp.25-27
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• 1962

It was previously reported that aqueous extracts of Radix Jingyu and Fructus ponciri, and also Lycaconitine, an alkaloid of Radix Jingyu, exhibit excellent effects as cattle tick repellent. The repost deals with the field teats carried out with these three drugs for their repellent effects against Korean goat ticks, nearly 99 per cent of which are composed of Haemaphysalis bispinosum and the rest of Boophilus microplus. The study revealed that Korean goat ticks are as susceptible to all three drugs tested as korean cattle ticks.

• Archives of Pharmacal Research
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• v.21 no.3
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• pp.344-347
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• 1998

To overcome multidrug resistance (MDR) in cancer chemotherapy, we prepared various plant extracts and searched for a component which is effective for inhibition of MDR. MDR inhibition activity was determined by measuring cytotoxicity to MDR cells using multidrug resistant human fibrocarcinoma KB V20C, which is resistant to 20 nM vincristine and expresses high level of mdr1 gene. Of various plant extracts, the MeOH extract of the root of Aconitum pseudo-laeve var. erectum was found to have potent inhibitory activity on MDR. The bioassayguided fractionation of the MeOH extract of the plant led to the isolation of an alkaloid, lycaconitine, as an active principle. And the $IC_{50}$ of lycaconitne for KB V20C cells was $74\mu{g}$/ml.