• ALGAE
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• v.39 no.2
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• pp.109-127
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• 2024

High production and efficient harvesting of microalgae containing high omega-3 levels are critical concerns for industrial use. Aeration can elevate production of some microalgae by providing CO₂ and O₂. However, it may lower the production of others by generating shear stress, causing severe cell damage. The mixotrophic dinoflagellate Gymnodinium smaydae is a new, promising microalga for omega-3 fatty acid production owing to its high docosahexaenoic acid content, and determining optimal conditions and methods for high omega-3 fatty acid production and efficient harvest using G. smaydae is crucial for its commercial utilization. Therefore, to determine whether continuous aeration is required, we measured densities of G. smaydae and the dinoflagellate prey Heterocapsa rotundata in a 100-L semi-continuous cultivation system under no aeration and continuous aeration conditions daily for 9 days. Furthermore, to determine the optimal conditions for harvesting through centrifugation, different rotational speeds of the continuous centrifuge and different flow rates of the pump injecting G. smaydae + H. rotundata cells into the centrifuge were tested. Under continuous aeration, G. smaydae production gradually decreased; however, without aeration, the production remained stable. Harvesting efficiency and the dry weights of omega-3 fatty acids of G. smaydae + H. rotundata cells at a rotational speed of 16,000 rpm were significantly higher than those at 2,000-8,000 rpm. However, these parameters did not significantly differ at injection pump flow rates of 1.0-4.0 L min^-1. The results of the present study provide a basis for optimized production and harvest conditions for G. smaydae and other microalgae.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.1
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• pp.39-51
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• 2012

Aerosol slurry samples were collected in 60-min interval using Korean Semi-continuous Elements in Aerosol Sampler (KSEAS) between May 19 and June 6, 2010 at an urban site of Gwangju. The $PM_{2.5}$ samples were collected with a flow rate of 16.7 L/min and particles are grown by condensation of water vapor in a condenser maintained at ${\sim}5^{\circ}C$ after saturation by direct injection of steam. The resulting droplets are collected in a liquid slurry with a airdroplet separator. Concentrations of 16 elements (Al, Fe, Mn, Ca, K, Cu, Zn, Pb, Cd, Cr, Ti, V, Ni, Co, As, Se) in the collected slurry samples were determined off-line by ICP-MS. KSEAS sample analysis encompassed the sampling periods for which 24-hr average elemental species concentrations were calculated for comparison with those derived from 24-hr integrated filter samples. Relationship between elemental species measured by two methods indicated high correlation coefficients (r), mostly greater than r of 0.80. However, we note that concentrations of Al, K, Ca, Mn, and Fe, which are often associated with crustal elemental particles, in the KSEAS samples, were substantially lower (1.4~11 times) than those found in the typical filter-based samples. This discrepancy is probably due to difficulties in transferring insoluble dust particles to the collection vials in the KSEAS. Temporal profiles of elemental concentrations indicate that some transient events in their concentrations are observed over the sampling periods. For the elemental species studied, atmospheric concentrations during the transient events increased by factors of 4 in Mn~80 in Zn, compared to their background levels. Principle component analyses were applied to the hourly KSEAS data sets to identify sources affecting the concentrations of the metal constituents observed. In this study, we conclude that hourly measurements for particle-bound elemental constituents were extremely useful for revealing the short-term variability in their concentrations and developing insights into their sources.

• Korean Chemical Engineering Research
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• v.56 no.1
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• pp.73-78
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• 2018

The main objective of this study was to evaluate the effects of acetate on the cultivation of anabena under mixotrophic condition. Four different types of acetates were used for the anebena cultivation. Among them, ethyl acetate was found to be the most effective and the growth rates linearly increased as the amount of ethyl acetate increased. When 40 mM of ethyl acetate was used, the highest values of specific growth rate of $0.979day^{-1}$ and maximum biomass productivity of $0.293g\;L^{-1}\;d^{-1}$ were obtained. On the contrary, input of acetic acid and butyl acetate inhibited the growth of anabena. For aeration tests, 0.54 vvm was optimum for anabena cultivation. For a semi-continuous cultivation test, ethyl acetate was used after 0.54 vvm test was finished. Then, test continued under 0.54 vvm and 40 mM of ethyl acetate. Lower specific growth rate and maximum biomass productivity were obtained compared to those from batch cultivation tests. However, the greatest maximum concentration of 5.91 g/L was obtained during the semi-continuous cultivation test.

• Korean Journal of Mathematics
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• v.13 no.1
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• pp.83-90
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• 2005

A.H.Hamel proved the Ekeland's principle in a locally convex Hausdorff topological vector spaces by constructing the norm and applying the Ekeland's principle in Banach spaces. In this paper we show that the Ekeland's principle in a locally convex Hausdorff topological vector spaces can be proved directly by applying the famous general principle of H.Br$\acute{e}$zis and F.E.Browder.

• Journal of applied mathematics & informatics
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• v.28 no.1_2
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• pp.487-498
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• 2010

For some $\phi$-sequences $\phi_1$, $\phi_2$ and $\phi_3$, and $\kappa$-function $\kappa_1$, $\kappa_2$ and $\kappa_3$ with $\kappa_1^{-1}(x)\kappa_2^{-1}(x)\;{\geq}\;\kappa_3^{-1}(x)$ for $x\;{\geq}\;0$, the Luxemburg norm is lower semi-continuous on ${\kappa}{\phi}BV_0$, and some specialized equivalent conditions are considered.

• Kyungpook Mathematical Journal
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• v.48 no.1
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• pp.1-14
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• 2008

In this paper, we investigate the existence of solutions of second order impulsive neutral functional differential inclusions which the nonlinearity F admits convex and non-convex values. Some results under weaker conditions are presented. Our results extend previous ones. The methods rely on a fixed point theorem for condensing multivalued maps and Schaefer's fixed point theorem combined with lower semi-continuous multivalued operators with decomposable values.

• Transactions of Materials Processing
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• v.23 no.3
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• pp.178-183
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• 2014

Continuous casting is a process where molten metal is solidified into a semi-finished billet on a large scale with either a rectangular or round cross section for subsequent processing. The use of continuous casting provides an opportunity for producing material on a practical industrial scale with lower cost than conventional casting. In the current study, the material was fabricated by continuous casting and subsequent extrusion. Tensile tests were conducted on continuous cast ZK60A after extrusion over a range of strain rates at temperatures from 473K to 623K. The alloy exhibits a quasi-superplastic behavior with a maximum recorded elongation of ~250% at 523K when tested with an initial strain rate of $10^{-5}/s$. The experiments give a strain rate sensitivity exponent of 0.3~0.4 and an activation energy of 108 kJ/mol. From the current investigation, it was found that the high-temperature plastic flow of the ZK60A is controlled by a dislocation viscous glide mechanism.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.6
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• pp.332-338
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• 2017

The purpose of this study was to determine optimum value of aeration, acetate dosage, and $CO_2$ input for the cultivation of Scenedesmus acuminatus. Highest specific growth rate and maximum biomass productivity was obtained by the aeration of 0.72 vvm and lower specific growth rates and maximum biomass productivity were obtained for other aeration tests. When putting 0.3 M of ammonium acetate in JM medium, the highest specific growth rate and maximum biomass productivity were obtained. $CO_2$ input tests were performed during semi-continuous culturing tests. The highest specific growth rate ($0.460d^{-1}$) and maximum biomass productivity ($0.936gL^{-1}d^{-1}$) were obtained after replacing 50% of solution with 0.3 M of acetate solution for $CO_2$ input tests. However, more dilutions after the first dilution resulted in lower specific growth rate and maximum biomass productivity. In aeration tests, the highest specific growth rate ($0.381d^{-1}$) and maximum biomass productivity ($0.253gL^{-1}d^{-1}$) were obtained when cultivating it with JM medium, but the specific growth rate and maximum biomass producitivty were significantly decreased when 50% of solution was replaced by acetate containing solution.

• Korean Chemical Engineering Research
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• v.53 no.5
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• pp.646-652
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• 2015

In this study transmembrane pressure (TMP) was measured with respect to permeate flux through the submerged flat sheet membrane for the emulsion and semi-synthetic cutting oil solutions. The effective area and nominal pore size of the used microfiltration membrane were $0.02m^2$ and $0.15{\mu}m$, respectively. The experiments were carried out simultaneously for run/stop (R/S) and sinusoidal flux continuous operation (SFCO) modes using two submerged membrane module in the reservoir. TMP for the case of SFCO was maintained under 60% of R/S, and the effect on TMP drop decreased as the permeate flux increased for emulsion cutting oil solution. Membrane fouling for the semisynthetic solution showing low turbidity was induced lower comparing to the emulsion solution. Also, the effect on TMP drop for SFCO decreased during long-term operation.

• Physical Therapy Korea
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• v.19 no.1
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• pp.28-36
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• 2012

This study used an unstable platform to change the support surface type and position of both lower limbs in order to determine changes in weight distribution and muscle including the vastus medialis, tibialis anterior, lateral hamstring, and lateral gastrocnemius of both lower limbs were evaluated during knee joint flexing and extending in a semi-squat movement in 32 hemiplegic patients. The support surface conditions applied to the lower limbs were divided into four categories: condition 1 had a stable platform for both lower limbs; condition 2 had an unstable platform for the non-hemiplegic side and a stable platform for the hemiplegic side; condition 3 had a stable platform for the non-hemiplegic side and an unstable platform for the hemiplegic side; and condition 4 had an unstable platform for both sides. The normalized EMG activity levels of muscles and weight bearing ratio of both sides in the four surface conditions were compared using repeated measures ANOVA. A significant increase was found in the weight support distribution for the hemiplegic side in flexing and extending sessions in condition 2 compared to the other conditions (p<.05). A statistically significant decrease in significant decrease in asymmetrical weight bearing in flexing and extending sessions was observed for condition 2 compared to the other conditions (p<.05). A similar significant decrease was found in differences in muscular activity for both lower limbs in condition 2 (p<.05). The muscular activity of the hemiplegic side, based on the support surface for each muscle showed a significantly greater increase in condition 2 (p<.05). An unstable platform for the non-hemiplegic side and a stable platform for the hemiplegic side therefore increased symmetry in terms of the weight support distribution rate and muscle activity of lower limbs in hemiplegic patients. The problem of postural control due to asymmetry in hemiplegic patients should be further studied with the aim of developing continuous effects of functional training based on the type and position of the support surfaces and functional improvement.