• Asian-Australasian Journal of Animal Sciences
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• 제28권2호
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• pp.180-187
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• 2015

This study was conducted to determine the effects of feeding by-product feed (BF)-based silage on the performance, blood metabolite parameters, and carcass characteristics of Hanwoo steers. The BF-based silage was composed of 50% spent mushroom substrate, 21% recycled poultry bedding, 15% cut ryegrass straw, 10.8% rice bran, 2% molasses, 0.6% bentonite, and 0.6% microbial additive (on a wet basis), and ensiled for over 5 d. Fifteen steers were allocated to three diets during the growing and fattening periods (3.1 and 9.8 months, respectively): a control diet (concentrate mix and free access to rice straw), a 50% BF-based silage diet (control diet+50% of maximum BF-based silage intake), and a 100% BF-based silage diet (the same amount of concentrate mix and ad libitum BF-based silage). The BF-based silage was fed during the growing and fattening periods, and was replaced with larger particles of rice straw during the finishing period. After 19.6 months of the whole period all the steers were slaughtered. Compared with feeding rice straw, feeding BF-based silage tended (p = 0.10) to increase the average daily gain (27%) and feed efficiency (18%) of the growing steers, caused by increased voluntary feed intake. Feeding BF-based silage had little effect on serum constituents, electrolytes, enzymes, or the blood cell profiles of fattening steers, except for low serum Ca and high blood urea concentrations (p<0.05). Feeding BF-based silage did not affect cold carcass weight, yield traits such as back fat thickness, longissimus muscle area, yield index or yield grade, or quality traits such as meat color, fat color, texture, maturity, marbling score, or quality grade. However, it improved good quality grade (1+ and 1++) appearance rates (60% for the control group vs 100% for the BF-based silage-fed groups). In conclusion, cheap BF-based silage could be successfully used as a good quality roughage source for beef cattle.

• Journal of Animal Science and Technology
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• 제62권4호
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• pp.429-437
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• 2020

This study was conducted to construct basic data for the selection of elite cows by analyzing the estimated breeding value (EBV) and accuracy using the pedigree of Hanwoo cows in Gyeongnam. The phenotype trait used in the analysis are the carcass weight (CWT), eye muscle area (EMA), backfat thickness (BFT) and marbling score (MS). The pedigree of the test group and reference group was collected to build a pedigree structure and a numeric relationship matrix (NRM). The EBV, genetic parameters and accuracy were estimated by applying NRM to the best linear unbiased prediction (BLUP) multiple-trait animal model of the BLUPF90 program. Looking at the pedigree structure of the test group, there were a total of 2,371 cows born between 2003 to 2009, of these 603 cows had basic registration (25%), 562 cows had pedigree registration (24%) and 1,206 cows had advanced registration (51%). The proportion of pedigree registered cows was relatively low but it gradually increased and reached a point of 20,847 cows (68%) between 2010 to 2017. Looking at the change in the EBV, the CWT improved from 4.992 kg to 9.885 kg, the EMA from 0.970 ㎠ to 2.466 ㎠, the BFT from -0.186 mm to -0.357 mm, and the MS from 0.328 to 0.559 points. As a result of genetic parameter estimation, the heritability of CWT, EMA, BFT, and MS were 0.587, 0.416, 0.476, and 0.571, respectively, and the accuracy of those were estimated to be 0.559, 0.551, 0.554, and 0.558, respectively. Selection of superior genetic breed and efficient improvement could be possible if cow ability verification is implemented by using the accurate pedigree of each individual in the farms.

• Asian-Australasian Journal of Animal Sciences
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• 제23권7호
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• pp.952-959
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• 2010

This study was conducted to evaluate growth, behavior and carcass traits of fattening Hanwoo (Korean native cattle) steers managed in different group sizes. A total of 48 animals, 6 months of age, were allocated to one of three group sizes and the experiment was conducted from 12 to 30 months of age. Groups were balanced for weight and the group sizes consisted of 4, 8, or 12 steers which were named 4sG, 8sG and 12sG, respectively. When animals were 12 months of age, initial fasted body weight (BW, $304.51{\pm}12.40\;kg$) was measured. All animals were housed at a constant space allowance of $8.82\;m^2$ per animal, and a feeder and drinker were provided per 4 animals. The whole fattening stage was divided into three phases: phase I (from 12 to 18 month of age), phase II (from 19 to 24 month of age), and phase III (from 25 to 30 month of age). Steers managed in 12sG showed low (p<0.05) growth rate and feed conversion rate (FCR) in phase I and phase II when compared to other treatment groups. However, this difference was not observed for the whole fattening phase (p>0.05). Steers managed in 4sG had a thick (p<0.05) ultrasound back fat thickness at 15 and 18 months of age. However, group size had no effect on meat yield and quality traits of area and marbling score. Animals managed in 8sG yielded a better meat grade of "A" than the "B" grade in other treatment group sizes. Lean color, fat color, firmness and maturity scores did not differ among group sizes. Hanwoo steers housed under 12sG spent less time on eating concentrate, relevant higher eating rate, less frequency of allogrooming, and more time on walking (p<0.05). It could be concluded that a large group size retarded growth rate and back fat thickness in the fattening stage, which was mainly focused on 15 and 18 months of age.

• Asian-Australasian Journal of Animal Sciences
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• 제28권8호
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• pp.1075-1083
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• 2015

Adipose tissue deposited within muscle fibers, known as intramuscular fat (IMF or marbling), is a major determinant of meat quality and thereby affects its economic value. The biological mechanisms that determine IMF content are therefore of interest. In this study, 48 genes involved in the bovine peroxisome proliferator-activated receptor signaling pathway, which is involved in lipid metabolism, were investigated to identify candidate genes associated with IMF in the longissimus dorsi of Hanwoo (Korean cattle). Ten genes, retinoid X receptor alpha, peroxisome proliferator-activated receptor gamma (PPARG), phospholipid transfer protein, stearoyl-CoA desaturase, nuclear receptor subfamily 1 group H member 3, fatty acid binding protein 3 (FABP3), carnitine palmitoyltransferase II, acyl-Coenzyme A dehydrogenase long chain (ACADL), acyl-Coenzyme A oxidase 2 branched chain, and fatty acid binding protein 4, showed significant effects with regard to IMF and were differentially expressed between the low- and high-marbled groups (p<0.05). Analysis of the gene co-expression network based on Pearson's correlation coefficients identified 10 up-regulated genes in the high-marbled group that formed a major cluster. Among these genes, the PPARG-FABP4 gene pair exhibited the strongest correlation in the network. Glycerol kinase was found to play a role in mediating activation of the differentially expressed genes. We categorized the 10 significantly differentially expressed genes into the corresponding downstream pathways and investigated the direct interactive relationships among these genes. We suggest that fatty acid oxidation is the major downstream pathway affecting IMF content. The PPARG/RXRA complex triggers activation of target genes involved in fatty acid oxidation resulting in increased triglyceride formation by ATP production. Our findings highlight candidate genes associated with the IMF content of the loin muscle of Korean cattle and provide insight into the biological mechanisms that determine adipose deposition within muscle.

• Asian-Australasian Journal of Animal Sciences
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• 제19권12호
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• pp.1678-1684
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• 2006

The objectives of this study were to estimate genetic parameters and sire breeding values for average daily gain (ADG) and carcass traits using sire-maternal grandsire model with REML approach, sire model with REML approach, sire model without relationships among sires and with REML and ANOVA approach, and to investigate advantages and disadvantages of these methods. Data were collected from 42,325 Japanese Black steers and heifers finished and slaughtered from 1991 to 2004. Traits analyzed in this study were average daily gain (ADG) during the fattening period, live weight at slaughter (LW), cold carcass weight (CW), estimated lean yield percentage (LYE), longissimus muscle area (LMA), subcutaneous fat thickness (SFT), rib thickness (RT), and marbling score (BMS). Bivariate analyses were also performed to obtain genetic and phenotypic correlation coefficients among traits. Estimated breeding values were obtained from each model, and simple and rank correlations among breeding values from each model were calculated. Estimates of heritability using the four models ranged from 0.25 to 0.31 in ADG, from 0.21 to 0.24 in LW, from 0.23 to 0.27 in CW, from 0.10 to 0.17 in DP, from 0.40 to 0.42 in LYE, from 0.19 to 0.31 in LMA, from 0.31 to 0.34 in SFT, from 0.26 to 0.33 in RT, and from 0.18 to 0.44 in BMS. The differences in heritability estimates using the four models seemed to be feasible in ADG, CW, DP, LMA, RT, and BMS. Genetic correlation coefficients of ADG with CW, SFT, RT and BMS were moderate to high and positive while the genetic correlation coefficients between ADG and LYE was low and negative. Correlation coefficients of BMS with SFT were negligible for both genetic and phenotypic correlations. The correlations of estimates evaluated from sire models with those from sire-maternal grandsire model were not large enough to convincing that breeding values using a sire model were corresponding to those using a sire-maternal grand sire model. If information of maternal grand sires are not available, the sire model with incomplete pedigree information included only sire of sire (Model 2) is optimal among the sire models evaluated in this study.

• Journal of Biomedical and Translational Research
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• 제19권4호
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• pp.116-124
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• 2018

Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP ${\times}$ Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (${\left|log2FC\right|}$ > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

• Journal of Animal Science and Technology
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• 제52권4호
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• pp.305-312
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• 2010

한우 거세 비육우에 출하 전 163일간 LPLT구(CP 12%, TDN 73%)와 LPHT구(CP : 12%, TDN : 75%), HPHT구(CP : 14%, TDN : 75%)로 배합사료를 급여한 사양시험결과 일당증체량은 LPHT구에 비하여 대조구와 HPHT구가 높은 것으로 조사되어 증체가 많았던 것으로 조사되었다(P<0.05). 사료요구율 역시 LPHT구에 비하여 LPLT구와 HPHT구의 사료이용효율이 좋았던 것으로 조사되었다(P<0.05). 도체율은 영양소의 추가적인 급여가 영향하지 않는 것으로 조사되었으나 수송감량에서는 HPHT구의 수송감량이 대조구와 LPHT구에 비하여 적은 것으로 조사되었다(P<0.05). 등지방두께와 배최장근단면적에서 HPHT구가 유의하게 개선되었으며, 냉도체중 및 육량지수에는 영향하지 않은 것으로 조사되었다. 육량등급의 수취에서 등지방두께와 정의 영향을 주는 배최장근단면적이 높았던 HPHT구가 LPLT구와 LPHT구에 비하여 유의하게 개선된 것으로 조사되었다(P<0.05). 육질형질 중 근내지방도는 대조구와 LPHT구에 비하여 HPHT구가 통계적으로 유의하게 증가하였고 이 영향으로 육질등급의 수취에서도 그대로 반영되어 유의하게 개선된 것으로 조사되었다(P<0.001). 그러나 다른 육질에 영향하는 항목들에서는 대조구와 처리구간의 차이가 인정되지 않았다. 지육경락가격은 LPLT구(17,904원)와 LPHT구(18,094원)에 비하여 HPHT구(18,899원)가 유의하게 수취금액이 높았다(P<0.01). 특히 최종 육질등급 출현율을 살펴보면 1등급이상 출현율에서 대조구는 79.2%로 나타난 반면 LPHT구는 72.7%가 출현되었고 HPHT구는 90.8%의 출현율을 나타내었다. 배최장근단면적의 이화학적 특성 분석에서 대조구와 LPHT구 및 HPHT구에서 통계적으로 유의한 수준의 변화는 없었다. 다만 HPHT구의 조지방함량이 증가하는 경향을 나타내었다(P=0.088). 등심 중의 지방산의 조성변화는 포화지방산의 경우 stearic acid 함량이 HPHT구에서 대조구에 비하여 유의하게 증가하였고, 불포화지방산은 Myristoleic acid 및 oleic acid의 함량이 대조구나 LPHT구에 비하여 HPHT구에서 유의한 증가를 보였다(P<0.05). 이러한 결과는 단일불포화 지방산함량 대비 포화지방산의 비율에도 영향을 미친 것으로 조사되었다. 본 연구에서 발육성적에서 HPHT구가 개선되었고 도체등급에서도 HPHT구가 현저히 개선되는 경향을 보였으며, 또한 도체의 지방산조성에서도 영향을 미친 것으로 조사되었다. 다만 한우 거세우에서 수행한 본 연구의 이 같은 발육성적과 도체의 성적을 고찰함에 있어서 비육용 배합사료의 원가 분석과 함께 시판되는 농후사료의 단가체계 등에 대한 고찰이 추가적으로 필요할 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제51권2호
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• pp.135-142
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• 2009

본 실험은 비육돈에 저에너지 사료를 급여했을 때 도살체중이 성장효율과 도체 품질에 미치는 영향을 조사하여 적정 도살체중을 추정할 목적으로 수행되었다. (Yorkshire $\times$ Landrace) $\times$ Duroc 교잡종 암퇘지와 거세돼지에게 3,060 kcal DE/kg의 저에너지사료를 생체중 80 kg부터 급여하여 110, 125, 혹은 135 kg에 도살한 다음 한국에서 선호도가 가장 높은 부위인 삼겹살과 선호도가 가장 낮은 뒷다리 및 등심에 대한 이화학적 특성과 관능검사를 실시하였다. 일당증체량과 일당사료섭취량은 공히 125 및 135 kg 구가 110 kg 구보다 높았고, 사료효율은 반대로 110 kg 구가 높았다(P<0.01). 등지방두께는 도살체중이 증가하면서 증가하였고(P<0.01와 P<0.05), 암퇘지보다 거세돼지에서 높아(P<0.01) 125 kg 거세돼지의 등지방두께(24.6 mm)는 135 kg 암퇘지의 수치(24.2 mm)와 비슷하였다. 삼겹살, 뒷다리 및 등심의 이화학적 특성은 도살체중의 영향을 거의 받지 않았으나, 110 kg과 125 kg 도살체중 사이 뒷다리의 적색도 증가(P<0.05)는 주목할만 하였다. 삼겹살에 대한 관능평가에서는 이 부위의 매우 중요한 품질 특성인 지방층:근육층 비율이 125 kg 도살체중에서 가장 높았으나(P<0.05) 전체적인 기호도는 도살체중과 무관하였다. 뒷다리와 등심부위에서는 상강도는 110 kg과 125 kg 도살체중 사이 증가한(P<0.01) 반면 색깔, 향, 이상취 및 육즙참출 정도는 도살체중의 영향을 받지 않았다. 또한 등심 부위의 전체적인 기호도는 110 kg과 125 kg 도살체중 사이 증가하였다(P<0.05). 가열육에 대한 관능평가에서는 110 kg과 125 kg는 색깔, 향, 이상취, 다즙성, 연도, 맛 및 전체적인 기호도 중 어느 품질 특성도 출하체중 변화로 인해 변하지 않았다. 결론적으로, 본 연구에 쓰인 저에너지 사료를 급여할 때 본 연구에 쓰인 돈군의 암퇘지와 거세돼지의 적정 도살체중은 각각 125~135 kg 및 125 kg 수준이다.

• 동물자원연구
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• 제29권4호
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• pp.172-182
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• 2018

본 연구는 저영양 비육돈 사양이 고영양 사양에 비해 도체 및 돈육의 질을 개선시킬 수 있을지를 조사하기 위해 수행되었다. 체중 약 50kg의 LYD 교잡종 암퇘지와 거세돼지 각각 68두씩을 8돈방에 배치하고 3.54Mcal DE/kg과 1.00% 라이신을 함유한 고영양사료 혹은 3.02Mcal DE/kg과 0.68% lysine을 함유한 저영양사료를 약 120kg까지 급여하고 도살하였다. 총 20개의 도체로부터 삼겹살, 등심, 뒷다리 및 목심을 잘라내고 삼겹살과 등심, 뒷다리 및 목심의 대표적인 근육의 질에 관한 이화학적 분석과 관능검사를 수행하였다. 일당증체량, 사료효율 및 등지방두께는 고에너지구보다 저에너지구가 낮았다(p<0.05). 목심의 가열감량, 경도 및 씹힘성 수치는 저영양구가 고영양구보다 낮았다. 신선육(근육)에 대한 관능평가에서 등심의 색깔, 상강도 및 기호도 평점은 저영양구가 고영양구보다 높았고, 삼겹살의 근육:지방 균형 평점은 저영양구가 고영양구보다 높은 경향을 나타냈다(p<0.10). 또한 뒷다리 가열육에 대한 관능평가에서는 풍미 및 다즙성 평점이 저영양구가 고영양구보다 높았다. 결론적으로, 이상의 결과는 저영양으로 비육돈을 사양하면 고영양 사양에 비해 성장성적은 저하되지만 도체 및 돈육의 질을 향상시킬 수도 있음을 시사한다.

• Journal of Animal Science and Technology
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• 제52권1호
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• pp.1-8
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• 2010

본 연구는 한우초음파생체단층촬영 자료를 이용하여 한우개량을 위한 기초자료로 활용코자 실시하였다. 당대검정우 1,125두에 대하여 초음파생체단층 촬영한 자료와 검정성적자료 및 12개월령에 거세한 후 30개월령까지 비육 후 출하하여 도체성적을 조사한 자료를 이용하였으며, 후대검정우는 921두에 대한 검정성적자료와 도체 성적자료를 이용하였다. 12개월령에 측정한 초음파생체단층 촬영 배최장근단면적과 등지 방두께의 유전력은 각각 0.57, 0.41로 추정되었으며, 24개월에서는 각각 0.57, 0.60으로 고도의 유전력을 보였다. 그러나, 초음파 측정 %지방함량 유전력 추정치에서는 12개월령과 24개월령에서 각각 0.14, 0.22로 저 중도의 유전력을 보였다. 12개월령에 초음파생체 단층 촬영한 배최장근단면적, 등지방두께, %지방함량과 도체형질의 배최장근단면적, 등지방두께, 근내지방도의 유전상관은 각각 0.616, 0.544, 0.501로 추정되었고, 24개월령에서는 각각 0.894, 0.937, 0.263으로 높은 유전상관을 보였다. 12개월령에 측정한 초음파 측정 %지방함량과 근내지방도에서 높은 유전상관으로 추정되었는데, 이는 24개월령에서는 환경적요인(사양기술 등)에 의해 영향을 많이 받은 것으로 사료되며, 선발에 활용할 경우 24개월령 측정치 보다는 12개월령 측정치를 이용하는 것이 더 바람직할 것으로 사료된다. 12개월령 체중과 도체형질을 이용하여 씨수소를 선발하는 선발지수와 이와 유전상관이 높은 초음파생체단층촬영 자료를 바탕으로 선발하는 선발지수간의 순위상관을 분석한 결과 12개월령에 초음파생체단층 촬영한 자료에서 후보씨수소 선발지수는 0.140, 보증씨수소 선발지수는 0.843으로 높은 순위상관을 보였다. 본 연구결과 실시간으로 배최장근단면적, 등지방두께, 근내지방도와 같은 도축하지 않아도 도체성적을 파악할 수 있는 초음파생체단층 촬영 기술의 장점을 이용하여 선발에 활용한다면 근내지방도는 24개월령에 측정하는 것보다 12개월령에 측정하는 것이 더 효율적이라고 사료되어지며, 향후 초음파생체단층기술에 관한 더 많은 연구를 통하여 종축선발에 활용한다면 씨수소의 조기선발이 가능할 것으로 사료된다.