• 한국정보디스플레이학회:학술대회논문집
• /
• 2000.01a
• /
• pp.199-200
• /
• 2000

Measurement of low cell gap and retardation by using a rotating polarizer method was proposed. For more precise calculation and measurement, we applied a retardation film that has large retardation value of $1\;{\mu}m$ to measurement system. From experiments, we proved that cell gap and retardation could be measured even though the values of those are so small.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.17 no.6
• /
• pp.633-640
• /
• 2004

We have studied color tracking of a fringe-field driven homogenously aligned nematic liquid crystal (LC) cell with negative dielectric anisotropy and compared it with other devices such as the twisted nematic(TN) and in-plane switching(IPS) modes. According to studies, the TN device shows bluish color at grey scale and even at a low retardation cell it cannot avoid color tracking. The authentic IPS device having cell retardation value of 0.23 ${\mu}{\textrm}{m}$ also shows bluish white color. However, the FFS device shows excellent color tracking characteristics even at high retardation value of the cell while keeping high transmittance and greenish white.

• Journal of the Optical Society of Korea
• /
• v.15 no.2
• /
• pp.161-167
• /
• 2011

In this paper, we propose an in-plane switching (IPS) mode for liquid crystal displays (LCDs) that, in principle, is free of retardation of the LC cell. Basically, the optical configuration of the LC cell consists of an A-plate and an LC layer for switching between the dark and bright states. We could achieve a fast response time compared with the conventional in-plane LC cell because the free retardation condition of the proposed LC cell enables us to reduce the cell gap even by quarter-wave retardation without any change of the optimized LC material in the transmissive mode. Experiments for verification of the proposed in-plane switching LC cells have shown a significant reduction of the rising time and falling time simultaneously due to the small cell gap. Furthermore, we also proposed an optical configuration for wide viewing property of the retardation free IPS LCD by applying the optical films. We proved the wide-view property of the retardation free IPS LCD by comparing its optical luminance with the calculated optical property of the conventional IPS LCD.

• Korean Journal of Optics and Photonics
• /
• v.11 no.3
• /
• pp.158-162
• /
• 2000

Measurement of low cell gap and retardation by using a rotating polarizer method was proposed. For more precise calculation and measurement, we applied a simple numerical calculation and a retardation film that has large retardation value of 1 ~m to measurement system. From experiments, we proved that cell gap and retardation could be measured even though the values of those are so small. small.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.18 no.8
• /
• pp.724-728
• /
• 2005

Electro-optic characteristics of reflective hybrid aligned liquid crystal (LC) cell driven by fringe field using a nematic LC with positive dielectric anisotropy have been studied. Optimized optical configurations are achieved by using a single polarizer, half-wave film and a cell with quarter-wave retardation. The simulation results shows an optimum cell retardation of $0.30{\mu}m$. This value may allow a practical cell gap larger than $3{\mu}m$, which makes it easy to control in the manufacturing process. Furthermore, this LC cell with optimized cell parameters shows low wavelength dispersion and the contrast ratio greater than 5 over exists about $100{\circ}$ in vertical direction and $160{\circ}$ in horizontal direction. Also, when using the LC with positive dielectric anisotropy rather than negative dielectric anisotropy, the display shows low power consumption and fast response time.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2005.07a
• /
• pp.411-414
• /
• 2005

We have developed a transflective liquid crystal display (LCD) with a single cell gap and a low twisted nematic (LTN) mode in combination with a self-integrated retardation layer. The retardation layer was made of UV curable liquid crystalline material in one step of photo masking process and has both the homeotropic and the planar parts. The proposed transflective configuration has advantages in a simple fabrication process, and the possibility for a single driving scheme due to the similarity between the transmittance and the reflectance.

• Biomedical Science Letters
• /
• v.23 no.2
• /
• pp.49-56
• /
• 2017

Fibroblast growth factor (FGF)-2 is one of the most effective growth factors to increase the growth rate of mesenchymal stem cells (MSCs). Previously, we reported that low dose of FGF-2 (1 ng/ml) induced proliferation of bone marrow-derived mesenchymal stem cells (BMSCs) through AKT and ERK activation resulting in reduction of autophagy and senescence, but not at a high dose. In this study, we investigated the effects of high dose FGF-2 (10 ng/ml) on proliferation, autophagy and senescence of BMSCs for long term cultures (i.e., 2 months). FGF-2 increased the growth rate of BMSCs in a dose dependent manner for a short term (3 days), while during long term cultures (2 months), population doubling time was increased and accumulated cell number was lower than control in BMSCs when cultured with 10 ng/ml of FGF-2. 10 ng/ml of FGF-2 induced immediate de-phosphorylation of ERK1/2, expression of LC3-II, and increase of senescence associated ${\beta}$-galactosidase (SA-${\beta}$-Gal, senescence marker) expression. In conclusion, we showed that 10 ng/ml of FGF-2 was inadequate for ex vivo expansion of BMSCs because 10 ng/ml of FGF-2 induced growth retardation via ERK1/2 de-phosphorylation and induction of autophagy and senescence in BMSCs.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.6
• /
• pp.788-794
• /
• 2015

Cationic liposomes have been actively used as gene delivery vehicles despite their unsatisfactory efficiencies because of their relatively low toxicity. In this study, we designed novel heterodimeric peptides as nonviral gene delivery systems from TAT and NLS peptides using cysteine-to-cysteine disulfide bonds between the two. Mixing these heterodimeric peptides with DNA before mixing with lipofectamine resulted in higher transfection efficiencies in MCF-7 breast cancer cells than mixing unmodified TAT, NLS, and a simple mixture of TAT and NLS with DNA, but did not show an adverse effect on cell viability. In gel retardation assays, the DNA binding affinities of heterodimeric peptides were stronger than NLS but weaker than TAT. However, this enhancement was only observed when heterodimeric peptides were premixed with DNA before being mixed with lipofectamine. The described novel transfection-enhancing peptide system produced by the heterodimerization of TAT and NLS peptides followed by simple mixing with DNA, increased the gene transfer efficiency of cationic lipids without enhancing cytotoxicity.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2003.07b
• /
• pp.972-975
• /
• 2003

We have performed computer simulation to obtain electro-optic characteristics of reflective hybrid aligned nematic liquid crystal displays driven by fringe field. The results show that the optimal retardation value of the cell is $0.289{\mu}m$, which allows for the cell to have a practical cell gap of larger than $3{\mu}m$ when manufacturing. A reflectance of the dark state is only 0.114% for an incident light 550nm. At this condition, the light efficiency of white state reaches 92.7%. Further, we have studied a new reflective display with polarizer, optical compensation with half plate, LC plus reflector. The display with optimized cell parameters shows low wavelength dispersion and the contrast ratio greater than 5 over exists about $120^{\circ}$ in vertical direction and $160^{\circ}$ in horizontal direction.

• Journal of Korean Neurosurgical Society
• /
• v.66 no.6
• /
• pp.642-651
• /
• 2023

Objective : Endothelial colony-forming cells (ECFCs) have been reported to play an important role in the pathogenesis of moyamoya disease (MMD). We have previously observed stagnant growth in MMD ECFCs with functional impairment of tubule formation. We aimed to verify the key regulators and related signaling pathways involved in the functional defects of MMD ECFCs. Methods : ECFCs were cultured from peripheral blood mononuclear cells of healthy volunteers (normal) and MMD patients. Low-density lipoproteins uptake, flow cytometry, high content screening, senescence-associated β-galactosidase, immunofluorescence, cell cycle, tubule formation, microarray, real-time quantitative polymerase chain reaction, small interfering RNA transfection, and western blot analyses were performed. Results : The acquisition of cells that can be cultured for a long time with the characteristics of late ECFCs was significantly lower in the MMD patients than the normal. Importantly, the MMD ECFCs showed decreased cellular proliferation with G1 cell cycle arrest and cellular senescence compared to the normal ECFCs. A pathway enrichment analysis demonstrated that the cell cycle pathway was the major enriched pathway, which is consistent with the results of the functional analysis of ECFCs. Among the genes associated with the cell cycle, cyclin-dependent kinase inhibitor 2A (CDKN2A) showed the highest expression in MMD ECFCs. Knockdown of CDKN2A in MMD ECFCs enhanced proliferation by reducing G1 cell cycle arrest and inhibiting senescence through the regulation of CDK4 and phospho retinoblastoma protein. Conclusion : Our study suggests that CDKN2A plays an important role in the growth retardation of MMD ECFCs by inducing cell cycle arrest and senescence.