• International Journal of Industrial Entomology and Biomaterials
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• 제27권1호
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• pp.142-158
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• 2013

The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is widely utilized for greenhouse pollination in South Korea. Understanding the magnitude of genetic diversity and geographic relationships is of fundamental importance for long term preservation and utilization. As a first step, we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to the "DNA barcode" region and the complete internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA from 88 individuals collected in nine South Korean localities. The complete ITS2 sequences were longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated 112-bp long repeats. The 658-bp long mtDNA sequences provided only six haplotypes with a maximum sequence divergence of 0.61% (4 bp), whereas the ITS sequences provided 84 sequence types with a maximum sequence divergence of 1.02% (21 sites). The combination of the current COI data with those of published data suggest that the B. ignitus in South Korea and China are genetically a large group, but those in Japan can be roughly separated into another group. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population were found to exist among the South Korean populations of B. ignitus, which suggests panmixia. This finding is consistent with our understanding of the dispersal capability of the species.

• The Plant Pathology Journal
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• 제25권2호
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• pp.142-150
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• 2009

The complete genome sequences of RNA3 and RNA4 of the 13 different Rice stripe virus (RSV) isolates were determined and characterized in this study to address the possible causes of the recent re-emergence of RSV that affected many rice fields in Korea. The genome size of each RNA segment varied among isolates and significant differences were observed in the intergenic region. There was up to 4% average divergence in the RNA4 nucleotide sequence among 13 Korean isolates and only 1.4% in the RNA3. Phylogenetic relationships among different Korean isolates revealed that there were at least 2 types of RNA3 and 4 distinct types of RNA4 genomes present in Korea. However, Korean isolates with one type of RNA3 predominate over the other while the occurrences of the RSV Korean isolates with the 4 types of RNA4 genome were not correlated to specific geographical areas. Results further indicate that RNA4 had diverged more than RNA3 and these differences in accumulation of mutations in the individual RNA segments indicate that genetic reassortment were likely to contribute to the genetic divergence in the 13 Korean isolates. All of the Korean-RNA3 sequences except for one isolate grouped with Chinese isolates (JY and Z). In contrast, the RNA 4 sequences segregated together with either Chinese (JY and Z) and Japanese (M and T) isolates but genetic relationships of Korean isolates- RNAs 3 and 4 segments to Chinese-Y isolate were low. Altogether, these results suggest that the occurrence of mixtures of RNAs 3 and 4 genotypes in the natural population of RSV may have contributed to the sudden outbreak in Korea.

• 한국수산과학회지
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• 제25권6호
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• pp.443-456
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• 1992

황해에서와 같은 선형의 양향성 대륙붕 해저 지형에서 저주파의 분산관계가 단주기$\cdot$단파까지 포함하는 장주기$\cdot$장파 경우에 대해 유도되었다. 선형의 양향성 대륙붕에서 장주기$\cdot$장파일 경우 Bessel 방정식이 유도되는데 비해 일반적인 경우에는 Hummer 방정식이 유도된다. Hummer 방정식의 해로 유도되는 confluent Hypergeometric 함수는 극한 경우에 여러 형태로 바뀐다. 단일한 대륙붕에서는 해안선과 수직한 방향의 대륵붕 규모가 Rossby deformation radius에 비해 많이 작을 때는 수평흐름의 수렴$\cdot$발산효과가 무시되지만 양향성 대륙붕에서는 수평흐름의 수린$\cdot$발산효과가 해안선과 수직한 방향의 대륙붕규모가 관계없이 파동역학에 결정적으로 중요하다. 수렴$\cdot$발산효과는 Kelvin 파를 포함시키며 대륙붕파의 파속을 감소시킨다. 끝으로 양향성 대륙붕의 비마찰 eigenfunction들의 직교가 증명되었다.

• 대한한의학방제학회지
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• 제24권4호
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• pp.323-333
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• 2016

Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.

• Molecules and Cells
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• 제25권4호
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• pp.510-522
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• 2008

To construct the molecular systematics of the genus Megoura (Hemiptera: Aphididae), DNA based-identification was performed using four mitochondrial and three nuclear DNA regions: partial cytochrome c oxidase I (COI), partial tRNA-leucine + cytochrome c oxidase II (tRNA/COII), cytochrome b (CytB), partial 12S rRNA + tRNA-valine + 16S rRNA (12S/16S), elongation factor-1 alpha ($EF1{\alpha}$), and the internal transcribed spacers 1 and 2 (ITS1, ITS2). Pairwise sequence divergences between taxa were compared, and phylogenetic analyses were performed based on each DNA region separately, and the combined datasets. COI, CytB, $EF1{\alpha}$, ITS1, and ITS2 were relatively effective in determining species and resolving their relationships. By contrast, the sequences of tRNA/COII and 12S/16S were not able to separate the closely related species. CytB and $EF1{\alpha}$ gave better resolution with higher average sequence divergences (4.7% for CytB, 5.2% for $EF1{\alpha}$). The sequence divergence of COI (3.0%) was moderate, and those of the two ITS regions (1.8% for ITS1, 2.0% for ITS2) were very low. Phylogenetic trees were constructed by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic analyses. The results indicated that the phylogenetic relationships between Megoura species were associated with their host preferences. Megoura brevipilosa and M. lespedezae living on Lespedeza were closely related, and M. nigra, monophagous on Vicia venosa, was rather different from M. crassicauda, M. litoralis, and M. viciae, which are oligophagous on Lathyrus and Vicia. The three populations of M. crassicauda formed a clade separated from M. litoralis and M. viciae. Nevertheless M. litoralis and M. viciae, which are morphologically similar, were not separated due to negligible sequence divergence. We discuss the phylogenetic relationships of the Megoura, and the usefulness of the seven DNA regions for determining the species level phylogeny of aphids.

• International Journal of Industrial Entomology and Biomaterials
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• 제44권2호
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• pp.55-64
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• 2022

Bombyx shini Park & Sohn, 2002 (Lepidoptera: Bombycidae), which was listed as an endemic species in South Korea has recently been renamed as the East Asian silk moth Rotunda rotundapex Miyata & Kishida, 1990 (Lepidoptera: Bombycidae). In this study, we sequenced the complete mitochondrial genome (mitogenome) of the R. rotundapex to announce genomic characteristics and to clarify its validity with a new name. The 15,294-bp long complete mitogenome comprises a typical set of genes [13 protein-coding genes (PCGs), 2 rRNA genes, and 22 tRNA genes] and one major noncoding, A + T-rich region, with an arrangement identical to that observed in most lepidopteran mitogenomes. The A/T content of the whole mitogenome was 79.22%; however, it varied among the regions/genes as follows: A + T-rich region, 91.62%; srRNA, 84.67%; lrRNA, 83.01%; tRNAs, 81.43%; and PCGs, 77.46%. Phylogenetic analyses of 35 species in the Bombycoidea superfamily showed the sister relationship between the families Sphingidae and Bombycidae s. str., with the higher nodal support [bootstrap support (BS) = 78%]. The Saturniidae was placed as the sister to the two families, but the nodal support for this relationship was low (BS = 53%). Current R. rotundapex was placed together with previously reported con-species with the highest nodal support, forming a separate clade from Bombyx, validating that B. shini can have a new genus name, Rotunda. However, the Korean R. rotundapex showed a substantial sequence divergence at 5.28% to that originated from an individual of type locality Taiwan in 1,459-bp of COI sequences. Considering such a high sequence divergence an additional study, which includes morphological and DNA barcoding data from further extensive distributional range maybe is needed for further robust taxonomic conclusion.

• Ocean and Polar Research
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• 제26권2호
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• pp.273-286
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• 2004

The effects of environmental forcing on autotrophic picoplankton distributional patterns were investigated for convergence ($5^{\circ}N$), divergence ($9^{\circ}N-10^{\circ}30'N$) and oligotrophic ($17^{\circ}N$) sites in the tropical eastern Pacific during 2001 and 2003 KODOS (Korea Deep Ocean Study) cruises. The distributions of picoplankton populations - Prochlorococcus, Synechococcus and picoeukaryotes algae - were determined by flow cytometric analyses. Latitudinal variations in abundance maxima, vertical profiles, integrated abundance (0-150 m), and estimated carbon biomass were contrasted for each site according to three hydrological conditions. Prochlorococcus showed consistently high abundance in the surface mixed layers of all sites at $1\;{\times}\;10^5{\sim}3\;{\times}\;10^5\;cells\;ml^{-1}$ and showed declining abundance below these layers. However, these decreasing rates were not particularly sharp showing considerably high abundance at $1\;{\times}\;10^4\;cells\;ml^{-1}$ or higher even at 100 m depth. Vertical profiles of Synechococcus and picoeukaryotes were generally parallel to each other in all sites. A clear abundance maximum was observed at divergence site at or slightly above the pycnocline depth. Higher abundance was observed at the surface mixed layer for convergence site but a sharp decrease was observed below the pycnocline. However, there was no significant abundance fluctuation with depth at more oligotrophic site ($17^{\circ}N$). Integrated cell abundance of Prochlorococcus was high in the oligotrophic site at $2.17\;{\times}\;10^{13}\;m^{-2}$, and low in the convergence site at $0.88\;{\times}\;10^{13}\;m^{-2}$. However, opposite pattern was observed for Synechococcus and picoeukaryotes where relatively high integrated cell abundance was shown in the convergence site. Estimated carbon biomass of Prochlorococcus contributed 30.4-80.3% of total autotrophic picoplankton carbon showing the highest contribution in the oligotrophic site and the lowest contribution in the convergence site. Synechococcus contribution of total autotrophic picoplantkon carbon biomass was lower than 5.8% for most of sites except the convergence site where Synechococcus contributed 23.2% of picoplankton carbon biomass. Carbon biomass of picoeukaryotes was 18.8-46.4% showing the highest carbon biomass at the convergence site. Overall, Prochlorococcus showed higher cell abundance and carbon biomass and exhibited different reaction to hydrological conditions when compare with the other two major autotrophic picoplankton groups.

• 한국수자원학회논문집
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• 제56권1호
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• pp.35-43
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• 2023

미래 유량분석은 기후변화 시나리오와 수문모형의 매개변수에 영향을 받고 이에 따른 불확실성이 존재한다. 본 연구에서는 Shared Socioeconomic Pathway (SSP) 시나리오와 수문모형 매개변수에 따른 미래 유량 분석의 불확실성을 분석하고자 하였다. SSP 시나리오 중, 대표적으로 사용되는 SSP2-4.5와 SSP5-8.5시나리오를 사용하였으며, 수문모형으로는 Soil and Water Assessment Tool (SWAT) 모형을 사용하였다. SWAT 모형의 매개변수는 SWAT-CUP을 이용해 관측된 유량 데이터에 따라 총 11개의 기간에 대해 매개변수 최적화를 각각 수행하였다. 그 후 분포의 차이를 계산 할 수 있는 Jensen-Shannon Divergence (JS-D)를 이용해 과거 유량 대비 미래 추정된 유량의 불확실성 분석을 수행하였다. 분석결과 미래 유량의 불확실성은 SSP5-8.5에서 SSP2-4.5보다 더 크게 분석되었으며, 가까운 미래(2021-2060년) 보다 먼 미래(2061-2100년)에서 더 크게 분석되었다. 강우-유출 분석은 수문모형 매개변수에 따라 88.5%-108.5%까지 차이가 발생하였으며, 이에 따라 미래 유량을 추정하는데 불확실성이 발생하였다. 본 연구에서의 수문 모형의 매개변수에 따른 미래 유량 추정의 불확실성은 평년 대비 유량이 적은 연도의 관측 유량 데이터를 이용한 매개변수를 이용할 시 불확실성이 크게 분석되었다. 또한 평년 대비 유량 변화가 큰 기간의 매개 변수일수록 미래 유량 추정의 불확실성이 크게 분석되었다.

• Asian Journal of Atmospheric Environment
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• 제6권3호
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• pp.222-233
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• 2012

The nocturnal low-level jet makes a significant impact on carbon and water exchanges and turbulent mixing processes in the atmospheric boundary layer. This study reports a case study of nocturnal surface fluxes such as $CO_2$ and water vapor in the surface layer observed at a flat and homogeneous site in the presence of low-level jets (LLJs). In particular, it documents the temporal evolution of the overlying jets and the coincident response of surface fluxes. The present study highlights several factors linking the evolution of low-level jets to surface fluxes: 1) wavelet analysis shows that turbulent fluxes have similar time scales with temporal scale of LLJ evolution; 2) turbulent mixing is enhanced during the transition period of low-level jets; and 3) $CO_2$, water vapor and heat show dissimilarity from momentum during the period. We also found that LLJ activity is related not only to turbulent motions but also to the divergence of mean flow. An examination of scalar profiles and turbulence data reveal that LLJs transport $CO_2$ and water vapor by advection in the stable boundary layer, suggesting that surface fluxes obtained from the micrometeorological method such as nocturnal boundary layer budget technique should carefully interpreted in the presence of LLJs.

• International Union of Geodesy and Geophysics Korean Journal of Geophysical Research
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• 제26권1호
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• pp.15-29
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• 1998

The numerical simulation of heavy precipitation event occurred in the central Korean Peninsula on July 26-28, 1996 was performed using the fine mesh model. ARPS (Advanced Regional Prediction System) developed by the CAPS (Center for Analysis and Prediction of Storms). Usually, the heavy rainfalls occurred at late July in the Korean Peninsula were difficult to predict, and showed very strong rainfall intensity. As results, they caused a great loss of life and property. As it usual, this case was unsuccessful to predict the location of rain band and the precipitation intensity with the coarse-mesh model. The same case was, however, simulated well with fine-mesh storm-scale model, ARPS. Moisture band at 850 hPa appeared along the Changma Front in the area of China through central Korea passed Yellow Sea. Also the low-level jet at 700 hPa existed in the Yellow Sea through central Korea and they together offered favorable condition to induce heavy rainfall in that area. The convective activities developed to a meso-scale convective system were observed at near the Yangtze River and moved to the central Korean Peninsula. Furthermore, the intrusion of warm and moist air, origninated from typhoon, into the Asia Continent might result in heavy rainfall formation through redistribution of moisture and heat. In the vertical circulation, the heavy rainfall was formed between the upper- and low-level jets, especially, the entrance region of the upper-level jet above the exit the region of the low-level jet. The low level convergence, the upper level divergence and the strong vertical wind were organized to the very north of the low level jet and concentrated on tens to hundreds km horizontal distance. These result represent the upper- and low-level jets are one of the most important reasons on the formation of heavy precipitation.