• 한국잠사곤충학회지
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• 제43권1호
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• pp.1-8
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• 2001

Nucleotide sequence in internal transcribed spacer (ITS) regions of ribosomal DNA among mulberry varieties (Morus species) were analyzed in order to identify the possibility of classification for the species. The variations in the ITS regions were compared among 9 mulberry varieties and one variety of Cudrania species as an outgroup. ITS 1 region of the varieties ranging from 219 to 220 bp in length was 49-50 bp shorter than ITS 2 region. Of 510 sites in the ITS 1 and 2 regions, 148 sites were potentially variable, of which 52% and 48% sites were distributed in ITS 1 and ITS 2 regions, respectively. By pairwise comparisons on the nucleotide sequences in the ITS 1 and 2 regions among 9 mulberry varieties, they were classified into 5 groups. Divergence values of the sequences, however, were considerably low ranging from 0 to 1.3%. Especially, there was no divergence among Backasipmunja, Chungilppong and Milsungpong and Jungyasang, Ssarigol II and Yulbon, respectively.

• 오토저널
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• 제10권2호
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• pp.61-69
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• 1988

The finite-difference three-dimensional boundary layer procedure of Chang and Patel is modified and applied to solve the boundary layer development on the automobile surface. The inviscid pressure distribution needed to solve the boundary layer equations is obtained by using a low order panel method. The plane of symmetry boundary layer exhibits the strong streamline divergence up to the midbody and convergence thereafter. The streamline divergence in front of the windshield helps the boundary layer to overcome the sever adverse pressure gradient and avoid the separation. The relaxation of the pressure right after the top of the wind-shield, on the other hand, makes the overly thinned boundary layer to readjust and prompts the streamlines to converge into the symmetry plane before the external streamlines do. The three-dimensional characteristics are less apparent after the midbody and the boundary layer is similar to that of the two-dimensional flow. The results of the off-plane-of-symmetry boundary layer are also presented.

• Animal cells and systems
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• 제2권4호
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• pp.451-454
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• 1998

Gulgastrura reticulosa, first described by Yosii (1966) as a monotypic new genus from a Korean limestone cave, was reviewed for its systematic position by c1adistic analysis of morphological characteristics, investigation of its intermaulting period and reproductive cycle as well as allozyme and 18S rDNA analysis. The great extent of divergence was strongly suggested by its combined lack of sensory organs (third antennal organ, postantennal organ, eyes, pseudocelli) with simultaneous development of an 'apical organ'at the tip of the antennae. The obvious divergence from any existing Collembola families was additionally supported by the extremely prolonged intermoulting period as well as by the low strap value it showed with Onychiuridae as obtained by 18S DNA sequence analysis. All these were considered Justifying the creation of a new family, Gulgastruridae, but still revealing more allied to Onychiuvidae rather than to Hypogastruridae.

• 한국광학회지
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• 제10권3호
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• pp.221-225
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• 1999

레이저선속의 퍼짐각을 개선하기 위하여 공진기 내부에 선속분할기를 갖는 양영역 공초점 불안정공진기 구리증기레이저를 구성하였다. 기하광학적 배율 M=30인 공진기에서 0.15 mard의 최소 퍼짐각을 얻었고, M=60인 공진기를 사용하여 0.1 mard 의 값을 얻을 수 있었다. 퍼짐각 개선과 관련하여 상대적인 원거리 출력밀도가 최대 130배 증가된 레이저 출력을 얻을 수 있었으며, 자체잠김현상을 보이는 펄스 형태의 레이저 발진이 관측되었다.

• 한국환경과학회지
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• 제21권12호
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• pp.1511-1521
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• 2012

An attempt is made to analyse characteristic features of heavy rainfalls which occur at the metropolitan area of the Korean peninsular the on- and off- Changma season. For this, two representative heavy rainfall episodes are selected; one is the on-Changma season wherein a torrential rain episode happened at Goyang city on 12 July 2006, and the other is the off-Changma season, a heavy rainfall event in Seoul on 21 September 2006. Both recorded considerable amounts of precipitation, over 250mm in a half-day, which greatly exceeded the amount expected by numerical prediction models at those times, and caused great damage to property and life in the affected area. Similarities in the characteristics of both episodes were shown by; the location of upper-level jet streak and divergence fields of the upper wind over heavy rainfall areas, significantly high equivalent potential temperatures in the low atmospheric layer due to the entrainment of hot and humid air by the low-level jet, and the existence of very dry air and cold air pool in the middle layer of the atmosphere at the peak time of the rainfall events. Among them, differences in dynamic features of the low-level jet and the position of rainfall area along the low-level jet are remarkable.

• 한국해양학회지:바다
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• 제13권3호
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• pp.178-189
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• 2008

2003년 7월, 2005년 8월 그리고 2007년 7월에 북동태평양의 발산대 해역($7^{\circ}{\sim}10.5^{\circ}N$)에서 무기영양염 분포와 재무기질화 비율 연구를 위한 조사를 수행하였다. 북적도 반류와 북적도 해류의 경계에서 형성되는 발산대는 라니냐 현상이 있었던 2007년 7월에 북위 $10^{\circ}N$에 위치하였으며, 용승 현상이 강하게 일어났다. 빈영양 환경의 특성을 갖는 표면 혼합층의 깊이는 2003년에 평균 46 m, 2005년에 평균 61 m 그리고 2007년에 평균 30 m 이었고, 표면 혼합층 이하에서는 용존산소 소모와 더불어 무기영양염 농도가 급격하게 증가하는 영양염약층이 형성됐다. 상층(수심 $0{\sim}100m$)에서 아질산염을 포함한 질산염의 총량은 2003년에 $5.51{\sim}21.71gN/m^2$(평균 $12.82gN/m^2$)의 범위를 나타냈고, 2005년에는 $5.62{\sim}8.46gN/m^2$(평균 $7.15gN/m^2$)의 범위를 그리고 2007년에는 $8.98~27.80 gN/m2$(평균 21.12 gN/m2)의 범위로 발산대가 형성된 지점에서 높은 값을 나타냈다. 인산염 총량과 규산염 총량 또한 아질산염을 포함한 질산염 총량 분포와 유사하였으며, 상층에서 파악된 아질산염을 포함한 질산염 총량에 대한 규산염 총량의 비율은 $0.87{\pm}0.11$ 이었다. 연구 해역에서 식물 플랑크톤 성장을 제한하는 무기영양염은 질소계 영양염으로(N/P ratio=14.6), 북적도 반류 지역에 비해 북적도 해류 지역에서 보다 낮은 농도를 나타냈다. 규산염 또한 낮은 농도로 존재하여 규소 제한 환경을 이루었다. 본 연구를 통해 분석된 재무기질화 비율은 $P/N/-O_2=1/14.6{\pm}1.1/100.4{\pm}8.8(23.44{\leq}Sigma-{\theta}{\leq}26.38)$로 Redfield stoichiometry($P/N/-O_2=1/16/138$) 보다는 낮았지만, 연구 해역 표층에서 재무기질화 과정을 설명하기에 충분하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권2호
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• pp.75-86
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• 2008

In order to understand geographic genetic variation and relationship among populations of the mason bee (Osmia cornifrons Radoszkowsky), which is used as pollinator for apple tree, we sequenced a portion of mitochondrial (mt) COI gene, which corresponds to "DNA Barcode" region (658 bp) from 81 O. cornifrons individuals collected over eight localities in Korea. The sequence data revealed overall moderate to low genetic diversity within species, with a maximum sequence divergence of 0.76%. Geographically, two haplotypes (BAROC01 and BAROC02) were widespread with a frequency of 82.7%, whereas several haplotypes were found in a locality as a single individual, suggesting that haplotype distribution can be summarized as coexistence of a few widespread haplotypes and several regionally restricted haplotypes. Overall, high rate of per generation female migration (Nm=$1.1{\sim}$infinite) and low level of geographic subdivision ($F_{ST}=0{\sim}0.315$) among localities were characteristic. Although two populations (p < 0.026) were genetically subdivided from the remaining localities, no clear polarity was observed. Taken together, the nature of genetic divergence of the mason bee populations is characterized as one that possessing moderate to low genetic diversity, high gene flow, and wide spread haplotypes with ahigh frequency, concordant with the capability of dispersal in connection with the lack of historical biogeographic barriers.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.62-69
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• 2015

The Asian cavity-nesting honeybee, Apis cerana (Hymenoptera: Apidae), has been extensively studied for its biogeography and genetic diversity, but the molecules utilized in past studies were mainly ~90 bp long mitochondrial non-coding sequences, located between $tRNA^{Leu}$ and COII. Thus, additional molecular markers may enrich our understanding of the biogeography and genetic diversity of this valuable bee species. In this study, we reviewed the public genome database to find introns of cDNA sequences, with the assumption that these introns may have less evolutionary constraints. The six introns selected were subjected to preliminary tests. Thereafter, two introns, titled White gene and MRJP9 gene, were selected. Sequencing of 552 clones from 184 individual bees showed a total of 222 and 141 sequence types in the White gene and MRJP9 gene introns, respectively. The sequence divergence ranged from 0.6% to 7.9% and from 0.26% to 17.6% in the White gene and the MRJP9 introns, respectively, indicating higher sequence divergence in both introns. Analysis of population genetic diversity for 16 populations originating from Korea, China, Vietnam, and Thailand shows that nucleotide diversity (π) ranges from 0.003117 to 0.025837 and from 0.016541 to 0.052468 in the White gene and MRJP9 introns, respectively. The highest π was found in a Vietnamese population for both intron sequences, whereas the nine Korean populations showed moderate to low sequence divergence. Considering the variability and diversity, these intron sequences can be useful as non-mitochondrial DNA-based molecular markers for future studies of population genetics.

• Journal of Positioning, Navigation, and Timing
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• 제7권3호
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• pp.155-163
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• 2018

This study examined methods to enhance navigation performance and reduce the divergence of navigation solutions that may occur in the event of global positioning system (GPS) failure by integrating the GPS/inertial navigation system (INS) with the three-dimensional (3D) magnetic vector measurements of a magnetometer. A magnetic heading aiding method that employs a magnetometer has been widely used to enhance the heading performance in low-cost GPS/INS navigation systems with insufficient observability. However, in the case of GPS failure, wrong heading information may further accelerate the divergence of the navigation solution. In this study, a method of integrating the 3D magnetic vector measurements of a magnetometer is proposed as a countermeasure for the case where the GPS fails. As the proposed method does not require attitude information for integration unlike the existing magnetic heading aiding method, it is applicable even in case of GPS failure. In addition, the existing magnetic heading aiding method utilizes only one-dimensional information in the heading direction, whereas the proposed method uses the two-dimensional attitude information of the magnetic vector, thus improving the observability of the system. To confirm the effect of the proposed method, simulation was performed for the normal operation and failure situation of GPS. The result confirmed that the proposed method improved the accuracy of the navigation solution and reduced the divergence speed of the navigation solution in the case of GPS failure, as compared with that of the existing method.

• Molecules and Cells
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• 제27권3호
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• pp.365-381
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• 2009

The chloroplast DNA sequences of Megaleranthis saniculifolia, an endemic and monotypic endangered plant species, were completed in this study (GenBank FJ597983). The genome is 159,924 bp in length. It harbors a pair of IR regions consisting of 26,608 bp each. The lengths of the LSC and SSC regions are 88,326 bp and 18,382 bp, respectively. The structural organizations, gene and intron contents, gene orders, AT contents, codon usages, and transcription units of the Megaleranthis chloroplast genome are similar to those of typical land plant cp DNAs. However, the detailed features of Megaleranthis chloroplast genomes are substantially different from that of Ranunculus, which belongs to the same family, the Ranunculaceae. First, the Megaleranthis cp DNA was 4,797 bp longer than that of Ranunculus due to an expanded IR region into the SSC region and duplicated sequence elements in several spacer regions of the Megaleranthis cp genome. Second, the chloroplast genomes of Megaleranthis and Ranunculus evidence 5.6% sequence divergence in the coding regions, 8.9% sequence divergence in the intron regions, and 18.7% sequence divergence in the intergenic spacer regions, respectively. In both the coding and noncoding regions, average nucleotide substitution rates differed markedly, depending on the genome position. Our data strongly implicate the positional effects of the evolutionary modes of chloroplast genes. The genes evidencing higher levels of base substitutions also have higher incidences of indel mutations and low Ka/Ks ratios. A total of 54 simple sequence repeat loci were identified from the Megaleranthis cp genome. The existence of rich cp SSR loci in the Megaleranthis cp genome provides a rare opportunity to study the population genetic structures of this endangered species. Our phylogenetic trees based on the two independent markers, the nuclear ITS and chloroplast MatK sequences, strongly support the inclusion of the Megaleranthis to the Trollius. Therefore, our molecular trees support Ohwi's original treatment of Megaleranthis saniculifolia to Trollius chosenensis Ohwi.