• Toxicological Research
• /
• v.25 no.3
• /
• pp.113-118
• /
• 2009

Cell death induced by menadione (vitamin K-3,2-methyl-1,4-naphthoquinone) has been investigated in human promyelocytic leukemia HL-60 cells. Menadione was found to induce both apoptosis and necrosis in HL-60 cells. Low concentration ($1{\sim}$50 ${\mu}$M) of menadione induced apoptotic cell death, which was demonstrated by typical DNA ladder patterns on agarose gel electrophoresis and flow cytometry analysis. In contrast, a high concentration of menadione (100 ${\mu}$M) induced necrotic cell death, which was demonstrated by DNA smear pattern in agarose gel electrophoresis. Necrotic cell death was accompanied with a great reduction of cell viability. Menadione activated caspase-3, as evidenced by both increased protease activity and proteolytic cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) into 85 kDa cleavage product. Caspase-3 activity was maximum at 50 ${\mu}$M of menadione, and very low at 100 ${\mu}$M of menadione. Taken together, our results showed that menadione induced mixed types of cell death, apoptosis at low concentrations and necrosis at high concentrations in HL-60 cells.

• Ocean and Polar Research
• /
• v.27 no.1
• /
• pp.45-58
• /
• 2005

Temperature, salinity, dissolved oxygen, COD, dissolved inorganic nitrogen(DIN), dissolved inorganic phosphorus (DIP), and chlorophyll were measured in the surface and bottom waters of Cheonsu Bay in April, August, December 2003, and Hay 2004. DIN showed a large seasonal variation, with higher values in summer and lower in spring. The significant decrease in DIN concentration was observed from April to May, which may imply the occurrence of spring phytoplankton bloom sometime in these periods. In contrast, DIP did not show distinct seasonal variation, with relatively low values compared with other coastal regions. The low DIP concentration in Cheonsu Bay is ascribed to a limited phosphorus input around Cheonsu Bay. The Nf ratios of Cheonsu Bay much higher than the Redfield ratio(16) in all season indicate that phytoplankton growth is limited by phosphorus. Based on low chlorophyll concentrations and eutrophication index, Cheonsu Bay has not been in eutrophic condition during our observation periods. In the artificial lakes located around Cheonsu Bay, however, chlorophyll concentrations were very high, mostly over $10{\mu}g\;l^{-1}$, indicating that they are now in severe eutrophic condition.

• Journal of Environmental Science International
• /
• v.27 no.6
• /
• pp.349-358
• /
• 2018

In this study, using coke dust from ironwork, the pulse pressure on a pulse air jet bag filter was investigated considering the influence of the pressure loss due to filtration velocity and pressure intervals. The research on the optimal pulse pressure prediction of a pulse air jet type bag filter using coke dust showed the following results. Pressure loss volatility produced by the pulse pressure under low dust concentration(0.5, $1g/m^3$) and low face velocity(1.25 m/min) was less than $10mmH_2O$. This suggests that the pulse pressure has a low impact on the pressure loss. In contrast, pressure loss volatility under high dust concentration($3g/m^3$) and high face velocity(1.75 m/min) was $25mmH_2O$. Therefore, pulse pressure with high dust concentration and high face velocity has a strong influence on the pressure loss volatility, compared to the condition of low dust concentration and low face velocity. The optimal pulse pressure of inlet dust concentration($0.5g/m^3$) was $6kg/cm^2$ under the same face velocity(1.75 m/min). As concentration increased from 1 to $2g/m^3$, the pulse pressure gradually reached $5kg/cm^2$ thus indicating that the pulse pressure($5kg/cm^2$) is pertinent at a high concentration($3g/m^3$). The pulse intervals: 20, 25 and 30 sec, which are relatively longer than 10 and 15 sec, corresponded to high pressure loss volatility produced by the pulse pressure. Furthermore, low pressure loss volatility was noted at $5kg/cm^2$ of the overall pulse pressure.

• Korean Journal of Environment and Ecology
• /
• v.34 no.5
• /
• pp.466-482
• /
• 2020

This study used 3D computational fluid dynamics (CFD) in the ENVI-met program to investigate how particulate matters (PM) generated on roads disperse through adjacent urban neighborhoods according to the urban development pattern. An urban area centered on a six-lane road in the vicinity of Miryang City Hall in Gyeongnam Province was selected to simulate the effect of the green space and building height on the PM concentration. The ENVI-met model considered the presence of green space and different building heights (high/low) on both sides of the road to examine the dispersion of PM. The result showed that the area of high-rise buildings and green space had the lowest PM concentration dispersed to the adjacent area, followed by the area of high-rise buildings and no green space. In contrast, the PM concentration remained relatively high for low-rise buildings, regardless of the green space. The reason for the low PM concentration in the area with high-rise buildings was a strong building wind, which caused PM to disperse to the outside, lowering the PM concentration quickly. These results indicate that the PM can disperse faster, and the PM concentration remains low in the urban neighborhood. On the other hand, green space had no significant effect on reducing PM in the urban neighborhood. In particular, when there are low-rise buildings on both sides of the road, the green space has no effect on the PM concentration in the urban neighborhood. Since this study considered only the case of PM emitted from the road, future studies should investigate other factors to figure out the dispersion model of PM and conduct on-site experiments.

• Proceedings of the Korean Environmental Health Society Conference
• /
• 2005.06a
• /
• pp.399-405
• /
• 2005

In this study, we investigated the gas chromatography (GC) and pulsed flame photometric detection (PFPD) system for the analysis of four major reduced S compounds including hydrogen sulfide ($H_2S)$; methyl mercaptan ($CH_3SH$); dimethyl sulfide (DMS); and dimethyl disulfide(DMDS) contained in environmental samples. To analyze these compounds in high concentration range (above ppb level), we developed a high mode analytical setting with the loop-injection system. By contrast, we also established a low mode setting for the analysis of low concentration samples (ppt-level samples from ambient air) by the combination with thermal desorption unit(TDU). Comparative analysis of both settings revealed that relative detection properties of four S compounds are systematic enough. The results of high mode analysis indicated that the patterns were systematic among compounds: H2S exhibited the lowest sensitivity, while DMBS showed the strongest one. The results were also compared in terms of sensitivity reductions for all compounds by dividing slope ratios between low and high mode system. Although low mode system exhibited significant reductions on the order of a few tens times, their detection characteristics were highly consistent as it was shown in the high mode setting. To learn more about absolute and relative relations between two different modes of S analysis, future studies may have to be directed to cover more complicated nature of GC/PFPD performance. Hydrogen sulfide($H_2S$) was over in summer about low level of olfactory sense 410 ppt, Methyl mercaptan(C$H_3SH$) was over in apring and summer about low level of olfactory sense 70, Dimethyl sulfide(DMS) was not over in four season about low level of olfactory sense 2,200 ppt. Carbon disulfide($CS_2$) was not over in four deason about Tow level of olfactory sense 210,000, Dimethyl disulfide(DMDS) was not over in summer about low level of olfactory sense2,000.

• Journal of the Korean Society of Radiology
• /
• v.14 no.5
• /
• pp.603-612
• /
• 2020

This study has a purpose to look into the effect of the relationship between the Tube current (mA) and SOD(Source to Object Distance), which is a parameter of lower limb angiography examination, and the dilution rate of the contrast medium concentration (300, 320, 350) on the image. To that end, using 3 mm vessel model water phantom, a vessel model custom made in the size of peripheral vessel diameter, this study measured relationships between change of parameters, such as tube current (mA), SOD and varying concentrations (300, 320, 350) of contrast medium dilution into SNR and CNR values while analyzing the coefficients of variance(cv<10). The software used to measure SNR and CNR values was Image J 1.50i from NIH (National Institutes of Health, USA). MPV (mean pixel value) and SD (standard deviation) were used after verifying numerically the image signal for region of interest (ROI) and background on phantom from the DICOM (digital imaging and communications in medicine) 3.0 file transmitted to PACS. As to contrast medium dilution by the change of tube current, when 146 mA and 102 mA were compared, For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As to contrast medium dilution by concentration for SOD change, when SOD's (32.5 cm and 22.5 cm) were compared,For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As to contrast medium dilution by concentration for SOD change, when SOD's (32.5 cm and 12.5 cm) were compared,For both SNR and CNR, the coefficient of variation value was less than 10 until the section of CM: N/S dilution (100% ~ 30% : 70%) but CM: N/S dilution rate (20%: 80% ~ 10% : 90%) the coefficient of variation was 10 or more. As a result, set a low tube current value in other tests or procedures including peripheral angiography of the lower extremities in the intervention, and make the table as close as possible to the image receiver, and adjust the contrast agent concentration (300) to CM: N/S dilution (30%: 70%). ) Is suggested as the most efficient way to obtain images with an appropriate concentration while simultaneously reducing the burden on the kidney and the burden on exposure.

• Food Science of Animal Resources
• /
• v.31 no.4
• /
• pp.543-550
• /
• 2011

Various concentrations of whey protein isolate (WPI), such as 0.1, 0.5, 1.0, 2.5, and 5.0%(w/v), containing 1.0%(w/v) eugenol were prepared by high speed homogenization to formulate nanoemulsions (NEs) and to investigate their antimicrobial activity. The results showed that particle size decreased according to increases in WPI concentration. Similarly, the ${\zeta}$-potential value was reduced to a negative charge when using WPI concentrations >0.1%(w/v). In contrast, no significant differences in particle size were observed during 1 mon of storage, except for the 0.1%(w/v) WPI NE. The ${\zeta}$-potential value depended on the increase in WPI concentration and storage duration, except for NE1 and NE5, suggesting that a low or high concentration of emulsifier was not effective for maintaining the droplet form of the eugenol NE. The results of an antibacterial effect investigation indicated that the growth of Escherichia coli was inhibited based on an increase in eugenol concentration in all NE formulations. Moreover, a membrane permeability study showed that total leakage content increased according to incubation time.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.20 no.3
• /
• pp.193-198
• /
• 2000

Plant characteristics of four winter crop hairy vetch, red clover, crimson clover and rye as green manure were evaluated at three harvesting time(Apri1 23, May 9, May 28) in spring to select superior winter green manure crop. Rye and hairy vetch had superior wintering ability and produced high dry matter compared with crimson clover and red clover. But N amount of rye was small because of its low plant N% despite of early dry matter accumulation. Wintering ability of crimson clover was very poor, so its dry matter decreased rapidly with low seeding rate. Hairy vetch had high N % (4%, C:N ratio 11) and produced high N yield among four plant. It is estimated that hairy vetch have high N-fixing ability because its N% was not changed largely despite of dry matter accumulation at late harvesting time contrast to the other legume. It was thought that hairy vetch was superior green manure crop in considering yield of dry matter and N. (Key words : Winter legumes, Hairy vetch, Green manure yield, Nitrogen concentration)

• Journal of the Korean Ceramic Society
• /
• v.44 no.9
• /
• pp.465-470
• /
• 2007

This paper presents an experimental study of the sulfate resistance of mortars and pastes exposed to sodium sulfate solutions up to one year. In order to check deterioration modes due to sulfate attack, the sodium sulfate solution was varied at three concentration steps (3,380, 10,140 and 33,800 ppm of $SO_4^{2-}$ ions), and maintained at ambient temperature. The tests include a visual examination, expansion and compressive strength loss measurements as well as x-ray diffraction tests. The experimental data indicated that the use of cement with a low $C_3A$ content and low silicate ratio has a beneficial effect on the sulfate attack of mortars. In contrast, the mortars with a high $C_3A$ content and high silicate ratio became severely degraded due to the formation of ettringite, gypsum and/or thaumasite in the cement matrix.

• Journal of the Mineralogical Society of Korea
• /
• v.28 no.2
• /
• pp.165-171
• /
• 2015

Dust samples collected from air in metropolitan areas of Busan and Ulsan were analyzed by phase contrast microscopy, phase contrast dispersion microscopy and SEM. Asbestos concentration in dust samples was lower than detection limit. Many fibrous materials were observed in dust samples, but most of them were organic fibers. Inorganic fibers such as rock fiber, ceramic fiber and gypsum were contained in the samples and non-fibrous minerals such as quartz, calcite and feldspar were also occasionally observed. Domestic law requires that asbestos in air dust is mainly analyzed by phase contrast microscopy. From this study, however, precise analysis of asbestos was almost impossible by this method only. As indicated in JIS method of Japan, therefore, count and identification of asbestos were more efficient by dispersion staining method after removing organic materials in samples by low temperature incinerator.