• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.31-32
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• 2005

Neoagaro-oligosaccharides are produced only by enzymatic degradation of agarose by ${\beta}-agarase.^{1)}$ Neoagaro-oligosaccharides inhibit the growth of bacteria, slow the rate of degradation of starch, are used as low-calorie additives to improve food quality, and have macrophage-stimulating activity. Furthermore, neoagarobiose is a rare reagent that has both moisturizing effect on skin and whitening effect on melanoma $cells.^{2)}$ An agar-degrading marine bacterium was isolated from the sea water at the northeast coast in Cheju island, Korea. The strain was gram negative, aerobic, and motile rod. The 16S rRNA of the strain had the closest match of 98% homology, with that from Agarivorans albus. On the basis of several phenotypic characters and a phylogenetic analysis, this strain was designated Agarivorans sp. JA-1. In solid agar plate, Agarivorans sp. JA-1 produced a diffusible agarase that caused agar softening around the colonies. Agarivorans sp. JA-1 was cultured for 36 hr in marine broth 2216 (Difco, USA) and the supernatant that containing an extracellular ${\beta}-agarase$ was prepared by centrifugation of culture media. The enzyme exhibited relatively strong activity at $40^{\circ}C$ and was stable up to $60^{\circ}C$. Using PCR primers derived from the ${\beta}-agarase$ gene of Vibrio sp., the gene encoding ${\beta}-agarase$ from Agarivorans sp. JA-1 was cloned and sequenced. The structural gene consists of 2931 bp encoding 976 amino acids with a predicted molecular weight of 107,360 Da. The deduced amino acid sequence showed 99% and 34% homology to $agaA^{2)}$ and $agaB^{2)}$ genes for ${\beta}-agarase$ from Vibrio sp., respectively. The expression plasmid for ${\beta}-agarase$ gene of Agarivorans sp. JA-1 is being constructed and the recombinant enzyme will be biochemically characterized.

• 한방비만학회지
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• 제2권1호
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• pp.89-94
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• 2002

2001년 1월 3일부터 2001년 4월 14일까지 고도의 복부비만으로 판정받은 1례의 환자에서 체감의이인탕의 한약복용, 초저열량식이요법(600kcal), 유산소운동(6km/h속도의 속보60분),행동수정요법(평소보다 50% 증가된 활동량), 전기지방분해침(Lipodren)40분, Infared therapy,이침, Aqua-pt(수압마사지)등을 입원 및 통원치료로 시행하여 비만지표, 신체사이즈, 간기능 검사, 복부 전산화단층촬영 상에서 유의한 변화를 관찰하였기에 보고하는 바이다.

• 한방비만학회지
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• 제22권1호
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• pp.86-92
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• 2022

26명의 환자에게 8~12주동안 산삼복합약침과 한약을 병행한 식이요법을 시행하여 체성분 변화에 미치는 영향을 알아보았다. 체성분 검사 결과 체중, BMI, 골격근량, 체지방량, 복부지방률, 내장지방단면적, 체지방률이 유의하게 감소하였고, 체중이 감소하면서 골격근량이 감소하긴 하였으나 체지방량 위주의 감소로 골격근의 비율은 오히려 유의하게 증가하였다. 태음조위탕을 증례에 맞게 가감하고, 하루 한 시간 혹은 만 보 걷기의 운동지도를 통해 하루 400~800 kal의 초저열량 식이로 인해 야기될 수 있는 부작용 중 일시적인 현기증과 무기력감 외에 탈모, 부종, 설사, 월경 이상 등은 관찰되지 않았다. 향후 체성분 변화뿐만 아니라 생화학적 검사나 골밀도 검사를 통한 장기적인 탐색이 필요할 것으로 생각한다.

• Clinical Nutrition Research
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• 제13권1호
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• pp.8-21
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• 2024

In this study, the effects of a 12-month multidisciplinary education program on the health status, dietary quality, and eating habits of children and adolescents attending community childcare centers were investigated. A total of 88 participants aged 7 to 17 years from 7 community childcare centers in Gyeonggi-do were enrolled. The intervention consisted of 12 multidisciplinary education sessions covering topics such as nutrition, exercise, and psychological education. All participants received the same education, and the effectiveness of the program was evaluated by categorizing them into a high participation group (HPG) and a low participation group (LPG) based on their participation rates. After intervention, in physical activities, moderate-intensity exercise was significantly reduced in the LPG, and there was no significant difference in psychological parameters. However, notable differences were observed in nutritional data. After intervention, intakes of calorie, carbohydrate, protein, and fat were significantly increased in both groups, and in particular, the change was found to be greater in HPG. Additionally, dietary fiber intake compared to the 2015 Korean Dietary Reference Intakes was increased in both groups. Daily food intake also increased dietary fiber intake in HPG, and meat and fruit intake was increased in LPG. In the nutrition quotient, there was a significant difference in HPG's pre- and post-scores in the diversity category, and in nutrient adequacy ratio (NAR), the NAR of phosphorus was increased in both groups. The findings of this study suggest that multidisciplinary education implemented at community childcare centers primarily enhanced nutrition-related factors rather than physical activity or psychological aspects.

• 한국식품영양과학회지
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• 제39권2호
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• pp.244-252
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• 2010

본 연구는 충북 도내에 거주하는 남녀 중학생 282명을 대상으로 24시간회상법을 이용하여 식이섬유 섭취 수준을 조사하고 식이섬유 주요급원식품을 알아보았다. 또한 식품섭취빈도조사를 이용하여 조사방법 간의 식이섬유 섭취수준을 비교, 분석하고 식이섬유섭취수준의 타당도를 검증하고자 하였다. 본 연구의 결과를 요약하면 다음과 같다. 조사대상자의 성별에 따른 열량 및 영양소 섭취량은 에너지필요추정량과 비교할 때 남학생은 75.4%, 여학생은 93.8%였다. 단백질은 평균필요량의 남녀 각각 85.8% 및 102.6%의 섭취수준을 보이며 남학생의 경우 다소 부족한 결과를 나타내었다. 한국인 영양섭취기준의 평균필요량이 남녀학생 모두 낮은 섭취량을 보인 영양소는 칼슘, 철분 및 엽산 순이다. 전반적으로 남녀학생 모두 영양소의 섭취수준이 낮은 것으로 나타났다. 24시간회상법에 의한 남학생의 1일 평균 식이섬유 섭취량은 $17.6\pm5.3$ g(54.8%), 여학생은 $16.5\pm4.8$ g(68.8%)으로 나타났으며 남녀학생 모두 충분섭취량에 매우 부족한 상태로 남녀학생의 유의적인 차이는 없었다. 식이섬유의 주요 급원식품 중 두 방법 간의 공통적인 섭취순위를 보인 식품은 1위 백미, 2위 배추김치이며 채소류와 곡류로부터 총 식이섬유의 68.44%의 섭취를 보였고 주요급원식품군별로 분류하면 채소류, 곡류와 그 제품, 과일류, 두류, 해조류 순이었다. 24시간회상법과 식품섭취빈도조사법 간의 Pearson 상관계수는 0.71로 높게 나타났으며, 유의적인 상관성(p<0.05)을 보였다. 백미가 0.82로 가장 높은 상관성을 보였고, 19종의 식품품목이 상관관계에서 유의성을 보였다. 식이섬유 섭취량의 Pearson 상관계수가 낮고 유의성이 없는 식품을 제외한 후 섭취량을 보정한 결과 상관성이 0.78로 높게 나타났고 유의적인 상관성(p<0.01)을 보였다. 두 방법에 의해 산출된 섭취수준에 따라 각각 4등급으로 분류하였을 때 보정 후 낮은 등급이 일치할 확률이 90.2%에서 92.4%로 높아졌고 평균 식이섬유의 Kappa값은 0.54에서 0.59로 높아졌다. 따라서 본 연구에서 개발된 식품섭취빈도 조사지는 비교적 높은 타당성을 보였으므로 조사지의 수정보완을 통해 청소년의 식이섬유섭취상태 파악에 유용하리라 생각된다. 또한 섭취량 보정을 통해 선정된 식품품목 19종을 기초로 하여 학생들의 학교급식메뉴와 기호도를 조사하고 급식과 연계하여 식품품목을 증가시키기 위한 과정이 선행되어야 할 것이다. 따라서 식품섭취조사지의 개발과 적극적인 활용방안을 위해서는 지속적인 연구가 필요하리라 사료된다.

• Journal of Nutrition and Health
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• 제6권3호
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• pp.37-45
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• 1973

The main character of the Korean diet has been found to be low in protein both quantity and quality and high in carbohydrate. The purpose of this survey was to study the amount of salt intake related to the dietary pattern in Korea. The nutrition survey was conducted in a mountainous farming area located in Auhchun-ri, Gaebuk-myon, Changsoo-gun, Chunbuk Province, February 14-19 in 1973 (7 days). The precise weighing method was used in evaluating the kinds of foods and nutrients intake for 24 households during a three day period. The physical examinations were performed by a doctor on 120 persons and a detailed biochemical test on both blood and urine was made on 42 persons over 40 years old. The results obtained are summarized as follows: (1) Average nutrients intake of an adult per day: calorie intake was 2,446 Cal and its components-protein(61.1g) was 10 percent, fat(12.9g) was 5 percent and carbohydrate(521g) contributed 85 percent of the total calories. Other nutrients-calcium (443mg), thiamine(1.09mg), riboflavin (0.90mg), niacin (14.4mg) and vitamin C (63.2mg) were lower than the recommended daily allowance but vitamin A(2,083 I.U.), iron(11mg) and phosphorous(998mg) were slightly higher than that. (2) To evaluate the nutritional deficiences, clinical examinations were conducted. Angular stomatitis was present in 16.7 percent of those examiners. No edema was found. The rate of osteoarthritis, hepatomegaly diseases appeared in 20 percent of the total subjects and the symptoms appeared highest among those Iron 50 to 59 years old. (3) The following chemical components of blood serum were analyzed and found to be within the normal range: glucose, blood urea nitrogen, uric acid, total protein, albumin, globulin, bilirubin, total cholesterol, inorganic phosphate, alkaline phosphatase, sodium, potassium, chloride, calcium and lecithine dehydrase. One case of each of the following were found: hyperglycemia, hypocholesterolemia, renal problem, hypoproteinaemia and diabetes mellitus, and two persons were classified as showing hypoglycemia and hyponaturemia. (4) The sodium content in urine was 199.6 mEq/L, potassium content was 24.6 mEq/L. The sugar, pH and specific gravity in the urine was shown to be normal.

• 동아시아식생활학회지
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• 제23권5호
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• pp.613-619
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• 2013

본 연구는 저칼로리 식품으로 알려져 있는 곤약과 약리작용이 뛰어난 쑥을 첨가한 곤약국수를 제조하고자 쑥의 건조방법에 따른 색도 변화와 곤약분말 농도, pH, 응고제의 종류에 따른 곤약 겔의 제조조건과 쑥의 첨가량에 따른 최적의 곤약국수의 제조방법을 조사하였다. 곤약국수에 사용할 쑥의 건조방법별 색도 변화 조사 결과, 1% $NaHCO_3$ 용액에 데친 후 건조한 방법(HADB, FDB)은 데치지 않은 방법(HAD, FD)에 비하여 쑥의 녹색유지에 효과적이었고 데친 후 동결건조한 방법(FDB)은 데친 후 열풍건조한 방법(HADB)에 비하여 선명한 녹색을 띄고 있었다. 따라서 쑥 가공처리에 있어서 1% $NaHCO_3$ 알칼리 용액에 데친 후, 동결건조방법이 쑥의 녹색을 유지하는데 가장 효과적이었다. 곤약 겔의 최적의 제조조건을 조사하고자 곤약분말의 농도, pH, 응고제의 종류에 따른 곤약 겔의 물성(견고성, 탄력성, 응집성, 점착성)을 측정하였으며, 그 결과, 분말농도는 9%, pH는 12, 응고제는 $Na_3PO_4$를 사용했을 때 물성치가 우수하게 나타났다. 쑥의 첨가량의 영향을 분석한 결과, 곤약분말에 대하여 1.5% 첨가가 색, 향, 맛 등 전반적인 기호도가 높게 나타나, 1.5% 첨가하는 것이 바람직할 것으로 판단되었다. 이상의 결과로부터 제시한 곤약의 제조 방법과 적절한 농도의 쑥을 첨가한 곤약국수를 제조하여 영양적, 물성적, 관능적으로 소비자들에게 긍정적인 반응을 보일 수 있는 제품이 제조될 수 있는 기초자료로 활용될 수 있다고 사료된다.

• 동아시아식생활학회지
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• 제25권1호
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• pp.176-182
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• 2015

타가토스의 함량을 달리한 머핀의 저장기간에 따른 수분 함량, 색도, 조직감을 분석하였으며, 관능평가를 실시하였다. 수분 함량은 저장기간에 따라 모든 실험군에서 감소하는 경향을 나타냈으며, 저장 2일째까지는 T25와 T50의 수분 함량이 유의적으로 높았으나, 3일째에는 유의적 차이를 나타내지 않았다. 색도의 경우, 황색도를 나타내는 b값이 T50에서 높게 나타났으며, 이는 관능평가에서도 같은 경향을 나타내, 타가토스가 설탕에 비해 갈변에 영향을 끼치는 것으로 분석되었다. 조직감 분석결과, 경도, 씹힘성, 깨짐성에서 저장기간에 따른 실험군별 유의적 차이가 나타났으며, 타가토스 함량이 높을수록 그 값이 높게 분석되었다. 탄력성과 응집성의 경우 T0과 T25는 유의적인 차이를 나타내지 않았다. 관능평가의 경우 껍질의 갈색 정도가 T25와 T50이 높게 나타났으며, 경도와 쓴맛의 경우 T0이 낮게 분석되었다. 그러나 내부색, 촉촉한 정도, 단맛, 향에 있어서는 실험군별 유의적 차이를 나타내지 않았고, 전체적 기호도 역시 실험군별 유의적 차이를 나타내지 않아 타가토스는 설탕 대비 50%까지 머핀 제조 시 첨가되어 사용될 수 있는 것으로 여겨진다.

• 대한지역사회영양학회지
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• 제13권4호
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• pp.476-488
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• 2008

This study investigates the relationship of smoking on daily intake of nutrients and snacks in the Chungnam and Daejeon high school students. Up to date scientific nutrition education and counseling programs in the regular school system is needed for a professional nutrition education teacher. The primary objective of this study was to provide useful information to nutrition education teachers. A survey was conducted with 400 high school students in the Chungnam and Daejeon areas. 381 out of 400 questionnaires were analyzed with SPSS 12.0K. The subjects were composed of 49.8% male, 50.1% female and 40.9% regular high school students, 59.1% business high school students and smokers 43.1%, non-smokers 56.9%. 43.4% of smokers had been smoking since middle school. On analysis of daily nutrient intakes, 16 out of 19 nutrients except animal calcium, Vitamin A and Vitamin C were much more consumed by the smoking group than the non-smoking group non-significantly. Especially vegetable fat and Vitamin E were higher in the smoking group than the non-smoking group (p<0.05). The intake ratio of carbohydrates: protein: fat was similar in the two groups (smoking group 55:15:29, nonsmoking group 56:15:28). Intakes of Vitamin B1 and potassium in comparison with the Korean dietary reference intakes(KDRI) were under 50% in both groups. However, sodium was taken over 200% compared to KDRI in both groups. Intakes of Vitamin C in the smoking group were as low as 76.5% in comparison to KDRI. Smokers need to increase the intakes of Vitamin C considering that smokers need to intake Vitamin C two times than non-smokers. Nutrient intakes from snacks in the smoking group were higher than the non-smoking group. Nutrients that originated from snacks which took over 20% among daily nutrient intakes were 12 nutrients(energy, fat, carbohydrate, calcium, P, Fe, K, Vitamin $B_1$, Vitamin $B_2$, Vitamin C, Vitamin E, dietary fiber) in the smoking group compared to 7 nutrients(energy, vegetable protein, fat, carbohydrate, calcium, Vitamin $B_2$, Vitamin C) in the non-smoking group. The smoking group was significantly paying more money for snacks each month than the non-smoking group was(p<0.01). Periods of consumption were irregular in the smoking group(p<0.05) and the smoking group was used to taking snacks in the morning compared to the non-smoking group. The smoking group preferred sweets and high calorie food over other snacks in comparison of the non-smoking group. The nonsmoking group had better eating habits than the smoking group.

• 대한지역사회영양학회지
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• 제24권2호
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• pp.106-116
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• 2019

Objectives: This study examined the status of children's favorite foods intake and the relationship with the policy environment based on the Special Act on Safety Control of Children's Dietary Life for suggesting a supportive policy strategy. Methods: The subjects were 4th grade students (n=1,638) in elementary school from 45 schools collected from seven areas (Seoul, Daegu, Daejeon, Gyeonggi, Chungnam, Jeonbuk, and Gyeongnam). The children participated in a self-administered questionnaire survey in class under the supervision of the teacher. The questionnaire consisted of items, such as social demographic characteristics, frequency of intake of the children's favorite foods, and policy cognition. A t-test and ANOVA were applied to explore the relationship between the frequency of children's favorite foods intake and policy cognition. The survey was implemented from August 2016 to September 2016. Results: For the boys, the frequency of 'high-calorie low nutrient foods intake' (HCLN) was significantly higher than that of the girls (p<0.01). For the children who received information on their favorite foods from the internet, the frequency of HCLN was higher than the other sources (p<0.01). The time of TV viewing and computer usage, and smartphone usage was associated with a higher frequency of HCLN, and a lower healthy favorite food intake (all p<0.001). The intake frequency of healthy favorite foods indicated a positive correlation with the policy cognition, including policy perception, usefulness, necessity and buying intention, and educational experience. Conclusions: This study showed a correlation with the frequency of children's favorite foods intake and policy. In particular, the frequency of children's healthy favorite foods intake indicated a meaningful relationship with the policy than the frequency of HCLN. This study also found that the consumption of children's healthy favorite foods was positively correlated with the educational experience. To develop a supportive policy for a good dietary environment for children, there is a need to focus on how to collaborate with multiple levels of influences, such as the national level, school level, and family.