• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1235-1243
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• 2020

The use of microalgal biomass as feedstock for biofuels has been discussed for decades as it provides a sustainable approach to producing fuels for the future. Nonetheless, its feasibility has not been established yet and various aspects of biomass applications such as CO₂ biofixation should also be explored. Therefore, in this study, the CO₂ biofixation and lipid/carbohydrate production potential of Chlorella sp. ABC-001 were examined under various nitrogen concentrations. The highest biomass productivity and CO₂ biofixation rate of 0.422 g/l/d and 0.683 g/l/d, respectively, were achieved under a nitrogen-rich condition (15 mM nitrate). Carbohydrate content was generally proportional to initial nitrate concentration and showed the highest value of 41.5% with 15 mM. However, lipid content showed an inverse relationship with nitrogen supplementation and showed the highest value of 47.4% with 2.5 mM. In consideration as feedstock for biofuels (bioethanol, biodiesel, and biogas), the sum of carbohydrate and lipid contents were examined and the highest value of 79.6% was achieved under low nitrogen condition (2.5 mM). For lipid-based biofuel production, low nitrogen supplementation should be pursued. However, considering the lower feasibility of biodiesel, pursuing CO₂ biofixation and the production of carbohydrate-based fuels under nitrogen-rich condition might be more rational. Thus, nitrogen status as a cultivation strategy must be optimized according to the objective, and this was confirmed with the promising alga Chlorella sp. ABC-001.

• 상하수도학회지
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• 제27권1호
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• pp.21-29
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• 2013

Present study was conducted to evaluate the performance of Anaerobic Hybrid Reactor (AHR) combined with two types of anaerobic attached growth reactors at mesophilic temperature ($37^{\circ}C$). The reactor was operated at the influent substrate condition of 19,400 mg/L soluble chemical oxygen demand (sCOD). The organic loading rate (OLR) and flow rate were varied in the range of $9.5{\sim}22.5kg/m^3$. day and 10.6 ~ 26.0 L/day respectively since start-up was done. The COD removal efficiency of 93 % was measured at the OLR of $14kg/m^3$. day in AHR. However a reduction in removal efficiency to as low as 85 % could have been related to a combined effect of high concentration suspended solids (SS) concentration over 3,800 mg/L. On the other hand the total COD removal efficiencies were measured to be 96.3 % and 96.2 % for AHR+APF and AHR+ADF respectively. The pH of the POME was adjusted to neutral range by using sodium bicarbonate at the initial stages of the reactor feed, later stages pH adjustment was not required as the pH was maintained in the desired neutral range due to self-buffering capacity of the reactor. The reactor proved to be economically acceptable and operationally stable. The biogas was measured to have $CH_4$ and $CO_2$ with a ratio of 35:65, and methane gas production rate was estimated to be $0.17{\sim}10.269L\;CH_4/g\;COD_{removed}$.

• Food Science and Biotechnology
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• 제16권3호
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• pp.482-484
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• 2007

To investigate production and hydrolysis of levan, the levansucrase enzymes from Zymomonas mobilis ATCC 10988 and Rahnella aquatilis ATCC 33071 were used. The optimum temperature of R. aquatilis levansucrase for levan formation was $37^{\circ}C$, whereas that of Z. mobilis was $4^{\circ}C$, under the experimental conditions. Both levansucrases also catalyzed the reverse levan hydrolysis reaction. Levan hydrolysis reactions from both levansucrases were temperature dependent; high temperature ($20^{\circ}C$) was more favorable than low temperature ($4^{\circ}C$) by 4 times. Fructose was the only product from hydrolysis reaction by both levansucrases, showing that both levansucrases mediated the hydrolysis reaction of exo-enzyme acting. In both enzymes, initial levan hydrolysis activity was almost accounted to 1% of initial levan formation activity. The results allow the estimation of the fructose release rate in enzyme processing conditions.

• 한국조경학회지
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• 제15권3호
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• pp.1-10
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• 1988

The initial selection of plants is the decisive step in the ultimate success of any landscape planting. This study intended to establish a reasonable selecting criteria of tree in the landscape through the holistic consideration of aesthetic, functional, cultural, operational and ecological viewpoints. The matrices of evaluating the fitness of tree were modulated to 10 categories ; availability from the established nursery. aesthetic value, indigenous to the locality, maintenance, soil fertility requirement, environmental tolerances. growth rate. wild-life food production. use of plants in design and user's likes. This 10 categories were weighted to their fitness as high(10 points), medium(8 points) and low(5points). Thus suitable plants were identified which got 70 more points from total 100 points. Such a method was believed as a rational process to get trust from client. to persuade uneducated client and to be free from designer's prejudice through a case study. But it is important to landscape designer to recognize the relationship between the rationality which prevails throughout the modern technical world and the intuitive aspects of design that are essential for developing creative solutions to encourage creativity as well as analytical responsibility.

• 원예과학기술지
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• 제29권6호
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• pp.592-599
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• 2011

Plum is a climacteric fruit and softening is a serious problem for storage and transportation. Thus $1{\mu}L{\cdot}L^{-1}$ of 1-methylcyclopropene (1-MCP) was applied to plums to prolong their shelf life and maintain quality. Japanese plums (Prunus salicina cv. Formosa) were stored at $20^{\circ}C$ and $0^{\circ}C$ for 14 days and 46 days respectively, with or without 1-MCP treatment. Fruits were treated with $1{\mu}L{\cdot}L^{-1}$ 1-MCP at $10^{\circ}C$ for 24 h. Ethylene production and respiration rate were strongly inhibited in 1-MCP-treated fruits at $20^{\circ}C$. It was also observed that there was less ethanol and acetaldehyde evaporation in 1-MCP-treated fruits stored at $20^{\circ}C$ compared to those in control fruits not treated with 1-MCP. Fruit qualities, such as firmness, titratable acidity (TA), skin color, and decay, changed more slowly in 1-MCP-treated fruits stored at $20^{\circ}C$ than in untreated fruits. There were no differences in the ethylene production or respiration rate between the groups of fruits stored at $0^{\circ}C$ throughout the experiment. Chilling injury was also inhibited by the application of 1-MCP during storage at $0^{\circ}C$. When the fruits stored at $0^{\circ}C$ with or without 1-MCP were transferred to $20^{\circ}C$ after 25 days, the differences in ethylene production and respiration rate, firmness, TA, TSS, and acetaldehyde and ethanol evaporation between the initial (after being stored at $0^{\circ}C$ for 25 days) and the final measurements (after being stored at $0^{\circ}C$ for 25 days and then transferred to $20^{\circ}C$ for three days) were lower in 1-MCP treated fruits than in non-treated fruits. The postharvest application of 1-MCP in Formosa plums showed positive effects at both $0^{\circ}C$ and $20^{\circ}C$ storage conditions with regard to quality, such as low ethylene production and low respiration rates, firmness, TA, ethanol, and acetaldehyde evaporation, chilling injury, and decay.

• KSBB Journal
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• 제14권3호
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• pp.284-290
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• 1999

우수한 셀룰로오스 생산 균주인 Acetobacter xylinum BRC5의 교반배양에 의한 셀룰로오스 생산성을 향상시키기 위하여 fed-batch 배양을 하였으며, 기질공급속도, 기질 공급량 및 용존 산소의 영향을 검토하였다. 초기 glucose 양을 변화시켜 회분배양하였을 때 glucose 농도가 10 및 20 g/L인 경우 셀룰로오스 생산량은 각각 2.05와 4.10 g/L이였으며 glucose에 대한 셀룰로오스 수율 (Yp/s)은 0.21이었다. 초기 glucose 농도 40g/L일 때 셀룰로오스 수율을 향상시키기 위해서 초기 glucose 농도 20 g/L에서 회분배양을 시작하여 glucose가 gluconic acid로 완전히 전환된 시점부터 추가적으로 glucose를 공급하여 fed-batch 배양기간에 glucose 공급속도는 셀룰로오스 생산성에 큰 영향을 미쳐 20g/L의 glucose를 2.22 g/L.h의 속도로 9시간 첨가하여 fed-batch 배양한 결과 셀룰로오스 생성량이 10 g/L로 가장 우수하여 초기 glucose 농도 20 g/L로 회분배양하였을 때 비하여 약 2배 증가하였으며, $Y_{P/S}$도 0.26으로 현저히 향상되었다. 또한 동일조건으로 fed-batch 배양하면서 glucose 공급량을 증가시켜 40g/L의 glucose를 추가적으로 첨가한 경우 셀룰로오스 생산량은 10.7g/L는 거의 증가되지 않았으며, $Y_{P/S}$가 0.18로 감소하였다. 이는 셀룰로오스 농도가 증가함에 따라 산소 공급이 부족하기 때문이므로 용존산소(DOT)를 2~15% 포화범위에서 조절하여 fed-batch 배양했을 때 DOT를 10% 수준으로 유지하면서 fed-batch 배양기간에 40g/L의 glucose를 추가공급 했을 때 셀룰로오스 생성량은 15.3 g/L로 증가되었고 이때 $Y_{P/S}$는 0.26로 향상되었다. 이는 DO를 제어하지 않는 경우에 비하여 셀룰로오스 생성량이 1.5배 증가한 결과이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.29-33
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• 2004

Low molecular weight silk fibroin (LMSF), which was prepared by hydrolysis of silk fibroin using high-temperature and high-pressure method, was found to inhibit the oxidation of L-3,4,-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase (EC 1.14.18.1). LMSF contained mostly free amino acids such as L-glycine, L-alanine, and L-serine and oligopeptides, mainly glycine-alanine dimer. As a result of analyzing the inhibition kinetics from Lineweaver-Burk plots, L-glycine and glycine-alanine dimer showed noncompetitive behavior while uncompetitive behavior was observed in L-alanine, and L-serine. When weight percent concentration of ${ID_50}$ was compared, L-glycine was most effective on the inhibition and LMSF was also good enough for the inhibition effect of tyrosinase activity. LMSF showed a mixed-type inhibition and the inhibitory mechanism of LMSF might be caused by free amino acids and oligopeptides. As a result of spectroscopic observation with time, initial rate of increase of DOPAchrome decreased remarkably and the time to reach maximum absorbance increased as an increase of the concentration of L-glycine, meaning that L-glycine made itself mainly responsible for the formation of chelate with ${Cu^2+}$ in tyrosinase. However, in case of L-alanine, L-serine, and especially glycine-alanine dimmer, the production of DOPAchrome after an arrival at maximum absorbance decreased, indicating the production of adducts through the reaction with DOPAquinone.

• 한국토양비료학회지
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• 제49권2호
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• pp.157-167
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• 2016

The interspecific estimation for tolerance capacities of upland crop species to excessive soil water stress in paddy field is significant in agricultural practices. Most of upland crops can be damaged by either excessive soil water or capillary rise of the water table during rainy season in paddy fields. The major objective of this study was to evaluate water stress of upland crops under different drainage classes in converted paddy field. This experiment was carried out in poorly drained soil (PDS) and imperfectly drained soil (IDS) of alluvial sloping area located at Toero-ri, Bubuk-myeon, Miryang-si, Gyeongsangnam-do. The soil was Gagog series, which was a member of the fine silty, mixed, nonacid, mesic family of Aeric Endoaquepts (Low Humic-Gley soils). Two drainage methods, namely under Open ditch drainage methods (ODM) and, Closed pipe drainage methods (PDM) were installed within 1-m position at the lower edge of the upper paddy fields. The results showed that sum of excess water days ($SWD_{30}$), which was used to represent the moisture stress index, was 42 days (the lowest) in the PDM compared with 110 days in the ODM. Most of upland crops were more susceptible to excessive soil water during panicle initial stage on more PDS than on IDS. Yield of upland crops in the PDM was continuously increased by the rate of 15.1% on sorghum, 15.4% foxtail millet, 53.6% proso millet, 49.6% soybean and 47.9% adzuki bean as compared in the ODM. The capacity for tolerance by excessive soil water based on yield of each upland crop in the poorly drained sloping paddy fields was the order of sorghum, soybean, foxtail millet, proso millet and adzuki bean. Therefore, Sorghum is relatively tolerant to excessive soil water conditions and, may be grown successfully in converted paddy field.

• 한국작물학회지
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• 제40권3호
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• pp.285-296
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• 1995

본 시험은 톨페스큐의 잎과 뿌리신장 및 동화산물의 생장점부위의 종적인 해부학적 고찰을 통해 세포분열, 신장, 그리고 성숙속도등 세포역학 및 세포의 생리적 반응에 대한 질소의 효과를 연구하고자 수행하였다. 공시품종은 경당 수량성이 높은 HYT 품종과 경당 수량성이 낮은 LYT 품종이었으며, 이들은 엽신장 및 분얼성 등 생리적 특성이 다른 품종이다. 1. 근관, 표피세포, 피층세포, 도관세포로 크게 구분되는 뿌리생장부위는 약 3.2mm로 잎생장부위(약 25~30 mm)보다 훨씬 짧았으며, 근관부위는 약 0.4~0.5 mm였다. 2. 근관세포의 경우 분열시 크기는 약 5 $\mu{m}$, 최종크기는 약 40 $\mu{m}$였으며 피층세포와 도관세포의 분열시 크기는 각가 8.5 $\mu{m}$와 13.0 $\mu{m}$, 최종 크기는 각각 120$\mu{m}$와 650$\mu{m}$로 큰 차이가 있었다. 3. 뿌리생장부위의 피층세포와 도관세포의 분열 직후 또는 최종크기는 질소시용수준에 영향을 받지 않았으나, 세포신장속도는 질소시용수준에 영향을 받아 높은 질소수준(200ppm)에서 보다 낮은 질소수준(50ppm)에서 약 2배 정도 빨랐다. 4. 뿌리세포의 분열속도는 질소의 영향을 받아 피층세포의 경우 50ppm N 수준에서 시간당 약 4.5 세포, 200ppm N 수준에서는 시간당 약 2.3 세포였으며, 도관세포의 경우는 각각 시간당 0.9 세포와 0.6 세포였다. 5. 뿌리세포가 분열 후 최대로 신장하기까지는 시간은 피층세포의 경우 50ppm N에서 약 21 시간, 200ppm N에서 약 43시간이 소요되었으며, 도관세포의 경우는 각각 약 37 시간과 73 시간이 소요되었다. 6. 뿌리생장부위의 피층세포와 도관세포의 비율을 조사한 결과, 세포 분열부위에서는 1:1이었으나, 세포신장부위에서는 그 비율이 증가해 생장점으로부터 1.0 mm 위치에서 5:1로 증가해 계속 유지되었다. 7. 뿌리생장에 대한 질소의 효과를 분석해 볼 때, 질소는 뿌리세포의 크기보다는 세포분열과 세포신장속도에 크게 영향함을 알 수 있었다.

• 한국환경보건학회지
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• 제34권2호
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• pp.175-182
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• 2008

This study investigates the optimal conditions for electrogenerated hydrogen peroxide production and the application of the electro-Fenton process using DSA electrodes. The influences of parameters for the hydrogen peroxide generation such as electrode materials, electrolyte concentration, current, pH, air flow rate and electrode distance were investigated using a laboratory scale batch reactor. The relative performance for hydrogen peroxide generation of each of the six electrodes is : Ru-Sn-Ti > Ru-Sn-Sb > Ru > Ir > Pt > Sn-Sb. Optimum NaCl dosage, current and air flow rate were 2.0 g/l, 12.5 A and 2 l/min, respectively. When the pH is low, hydrogen peroxide concentration was high. Electrode distance dos not effect to a hydrogen peroxide generation. A complete color removal was obtained for RhB (200 mg/l) at the 8 min mark of the electro-Fenton process under optimum operation conditions of $Fe^{2+}$ 0.105 g/l and 5.0 A. The electro-Fenton process increased initial reaction and decreased final reaction time. However the effect was not high.