• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1599-1604
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• 2022

Storage stability of freeze-dried lactic acid bacteria is a critical factor for their cost-effectiveness. Long-term storage of lactic acid bacteria enables microbial industry to reduce distribution costs. Herein, we investigated the effect of cold adaptation under supercooling conditions at -5℃ on the viability of Leuconostoc mesenteroides WiKim32 during the freeze-drying process and subsequent storage. Cold adaptation increased the thickness of exopolysaccharides (EPS) and improved the viability of freeze-dried Leu. mesenteroides WiKim32. Compared to non-adapted cells, cold-adapted cells showed a 35.4% increase in EPS thickness under supercooling conditions. The viability of EPS-hydrolyzed cells was lower than that of untreated cells, implying that EPS plays a role in protection during the freeze-drying process. Cold adaptation increased the storage stability of freeze-dried Leu. mesenteroides WiKim32. Fifty-six days after storage, the highest viability (71.3%) was achieved with cold adaptation at -5℃. When EPS-containing broth was added prior to the freeze-drying process, the viability further increased to 82.7%. These results imply that cold adaptation by supercooling pretreatment would be a good strategy for the long-term storage of Leu. mesenteroides WiKim32.

• 한국자원식물학회지
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• 제36권6호
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• pp.562-570
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• 2023

The droplet-vitrification technique for cryopreservation has proven successful across a diverse range of germplasm, ensuring safe and effective long term preservation. In this study, we investigate an effective cryopreservation protocol using the droplet-vitrification technique for shoot tips of Freesia hybrida cultivars 'Sunny Gold' and 'Sweet Lemon'. To determine optimal conditions for Freesia cryopreservation, we employed a carefully selected standard procedure along with additional treatments and alternative solutions. For 'Sunny Gold', the highest regrowth rate of 24% was achieved when shoot tips underwent dehydration with PVS3 solution for 120 minutes before direct immersion in liquid nitrogen (LN) for 1 hour, coupled with a standard protocol involving a two-step preculture with 0.3 M - 0.5 M sucrose, loading with C4 for 40 minutes, and unloading with 0.8 M sucrose for 40 minutes. In the case of 'Sweet Lemon,' regrowth of cryopreserved shoot tips was observed with dehydration treatments, including PVS2 (A3) for 60 minutes and PVS3 (B1) for 60 minutes, as well as longer exposure. The results reflect the distinct sensitivity of shoot tips to chemical toxicity and osmotic stress in these two genotypes. This study provides valuable evidence to consistently enhance the effectiveness of cryopreservation methods for the long-term conservation of Freesia germplasm.

• International Journal of Advanced Culture Technology
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• 제11권4호
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• pp.393-405
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• 2023

Accurate prediction of wind speed and power is vital for enhancing the efficiency of wind energy systems. Numerous solutions have been implemented to date, demonstrating their potential to improve forecasting. Among these, deep learning is perceived as a revolutionary approach in the field. However, despite their effectiveness, the noise present in the collected data remains a significant challenge. This noise has the potential to diminish the performance of these algorithms, leading to inaccurate predictions. In response to this, this study explores a novel feature engineering approach. This approach involves altering the data input shape in both Convolutional Neural Network-Long Short-Term Memory (CNN-LSTM) and Autoregressive models for various forecasting horizons. The results reveal substantial enhancements in model resilience against noise resulting from step increases in data. The approach could achieve an impressive 83% accuracy in predicting unseen data up to the 24th steps. Furthermore, this method consistently provides high accuracy for short, mid, and long-term forecasts, outperforming the performance of individual models. These findings pave the way for further research on noise reduction strategies at different forecasting horizons through shape-wise feature engineering.

• 한국음향학회지
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• 제25권8호
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• pp.375-382
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• 2006

본 논문에서는 음성신호에 포함된 감정정보를 자동으로 식별하는 방법과 특정 감정을 검출하는 방법에 대해 다룬다. 자동 감정식별 및 검출을 위해 장구간 (long-term) 음향 특징을 사용하였고, F-score 기반의 특징선택 기법을 적용하여 최적의 특징 파라미터들을 선정하였다. 기존의 일반적인 SVM을 확률출력 SVM으로 변환하여 감정식별 및 감정검출 시스템을 구축하였으며, 가설검정에 기반한 감정검출을 위해 세 가지의 대수 우도비 (log-likelihood) 근사법을 제안하여 그 성능을 비교하였다. SUSAS 데이터베이스를 사용한 실험 결과, F-score를 이용한 특징선택 기법에 의해 감정식별 성능이 향상되었으며, 확률출력 SVM의 유효성을 검증할 수 있었다. 감정검출의 경우, 제안한 방법에 의해 91.3%의 정확도로 화난 감정을 검출할 수 있었다.

• 대한간호학회지
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• 제42권1호
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• pp.85-94
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• 2012

Purpose: This study was done to examine the effects of nurses' perception of servant leadership on leader effectiveness, satisfaction and promoting additional effort. The focus was the mediating effects of leader trust and value congruence. Methods: Data were collected from 361 RN-BSN students and nurses participating in nationally attended in-service training programs. Data were analyzed using descriptive statistics and structural analysis with SPSS 17.0 windows program and Amos 7.0. Results: Direct effects of nurses' perception of servant leadership were negative, but mediating effects of trust and value congruency were positively correlated with leader effectiveness, satisfaction and additional effort, that is servant leadership should be effective through mediating factors. Conclusion: The study results indicate that if the middle managers of nurses can build leader trust and value congruency between nurses through servant leadership, leader effectiveness, satisfaction and additional effort on the part of the nurses could result in a positive change in the long term.

• Journal of Nutrition and Health
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• 제37권7호
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• pp.576-584
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• 2004

The purpose of this study was to evaluate the effects of Web-based nutrition education program for junior and senior high school students. Three materials, which were printed materials on Group I, WBI (Web-Based Instruction) leaflet on Group II and the web-based nutrition education program developed in the antecedent research, were applied for 564 students in 3 junior high school and 4 senior high school in Seoul during 4 weeks. Pre-test was done from October 21 to October 26, 2002 and post-test was done from November 25 to December 13, 2002. The statistical data analysis was completed using SPSS WIN (ver. 11.0) for descriptive analysis, t-test and ANOVA. According to the evaluation of effectiveness for web-based nutrition education program, nutrition knowledge, food attitude, food habit and nutrient intake were not significantly improved but positively. And on the evaluation of effectiveness for nutrition education program by materials, WBI was more effective educational materials than WBI leaflet and printed materials. Consequently nutrition knowledge, food attitude, food habit and nutrient intake were improved positively but not significantly using WBI, which was due to the instructional period of the only 4 weeks. Therefore long-term instruction should be required for effective nutrition program, as well as more WBI nutrition education program for high school students would need to be developed in the near future in that WBI was effective material on nutrition education.

• 성인간호학회지
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• 제21권2호
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• pp.155-166
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• 2009

Purpose: This study developed and evaluated a systematic intervention among medical ICU nurses for preventing ventilator-associated pneumonia (hereafter VAP). Methods: A VAP prevention program was proposed based on a literature review, revised to fit the target situation, and validated. It was composed of one-time interventions including education, pamphlets, hand cultures, and a quiz event, as well as repeated interventions such as posters, reminders, posting hand culture results, and performance feedback. A simulated control group pretest-posttest design was used to verify the effectiveness of the VAP control program. The incidence of VAP among ICU patients was measured both during 3 months before (n=80) and during 3 months after (n=75) intervention. Results: The VAP prevention program's effectiveness, with a pre-intervention VAP rate of 17.38 and post-intervention rate of 11.04 per 1,000 ventilator days, showed a clinical tendency to decrease, but the difference was not statistically significant (p=.750). Conclusion: A VAP prevention program of multiple interventions can be useful in decreasing the VAP rate. Given that the monthly decrease in the VAP rate was not considered statistically significant, long-term research needs to be done. Additionally, since this study targeted only nurses, it is suggested that future research targets other health care workers who can influence VAP rates.

• Nuclear Engineering and Technology
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• 제52권7호
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• pp.1429-1435
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• 2020

Applying an accurate parametric prediction model to identify abnormal or false pressurizer water levels (PWLs) is critical to the safe operation of marine pressurized water reactors (PWRs). Recently, deep-learning-based models have proved to be a powerful feature extractor to perform high-accuracy prediction. However, the effectiveness of models still suffers from two issues in PWL prediction: the correlations shifting over time between PWL and other feature parameters, and the example imbalance between fluctuation examples (minority) and stable examples (majority). To address these problems, we propose a cost-sensitive mechanism to facilitate the model to learn the feature representation of later examples and fluctuation examples. By weighting the standard mean square error loss with a cost-sensitive factor, we develop a Cost-Sensitive Long Short-Term Memory (CSLSTM) model to predict the PWL of PWRs. The overall performance of the CSLSTM is assessed by a variety of evaluation metrics with the experimental data collected from a marine PWR simulator. The comparisons with the Long Short-Term Memory (LSTM) model and the Support Vector Regression (SVR) model demonstrate the effectiveness of the CSLSTM.

• 한국실천공학교육학회논문지
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• 제4권2호
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• pp.98-103
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• 2012

우리나라에서 최초로 실행되는 한기대 IPP 제도는 실행과정에서 많은 어려움이 예상되며, 그 효과성 또한 아직 불확실하다. 따라서 한국형 IPP 제도의 실효성을 평가하고 프로그램 운영체계 및 과정에 대한 개선방안을 모색하기 위해 체계적인 성과평가모형의 개발이 절실하게 요구된다. 본 연구에서는 IPP 제도의 성과평가 방안을 Kirkpatrick의 4단계 평가모형에 기반하여 제시한다. 이를 위해 외국의 다양한 Co-op 평가사례를 분석하여 평가방법과 평가지표를 찾아내고, 이를 Kirkpatrick의 4단계(반응평가, 학습평가, 행동평가, 그리고 결과평가)에 매핑한다. 이러한 성과평가모형을 통해 IPP 프로그램의 성과와 문제점을 파악함으로써 고용노동부나 대학 내 학부 과 등 관련 이해관계자 집단들을 설득하고 지원을 얻는데 활용할 수 있을 뿐만 아니라 IPP 제도의 효과를 극대화시키는데 기여할 수 있을 것으로 예상된다.

• 한국융합학회논문지
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• 제9권11호
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• pp.393-402
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• 2018

최근 장기요양보험제도에 도입된 시장화와 공급주체 다원화의 영향으로 조직성과에 대한 관심이 높아지고 있다. 본 연구는 장기요양시설의 조직성과에 요양보호사의 소명의식의 영향을 분석하고 그 경로에서 직무만족의 매개효과를 검증하기 위하여 서울시와 경기도의 요양보호사를 대상으로 자료를 수집하였다. 다중회귀분석으로 소명의식이 직무만족과 조직성과에 미치는 영향을 분석하였고 위계적 회귀분석으로 직무분석의 매개효과를 검증하였다. 분석 결과 소명의식은 조직성과와 직무만족에 정(+)의 영향을 미쳤고, 직무만족은 소명의식과 효과성 사이에서 완전매개효과를, 소명의식과 효율성 사이에서는 부분매개효과를 나타냈다. 이를 바탕으로 직무만족 관리를 통하여 효과성을 제고하고, 직무만족과 소명의식을 관리하여 효율성을 관리할 필요성을 논의하였다.