• Title/Summary/Keyword: Long-rod

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Isolation and Characterization of Watermelon Isolate of Cucumber green mottle mosaic virus(CGMMV-HY1) from Watermelon Plants with Severe Mottle Mosaic Symptoms

  • Shim, Chang-Ki;Han, Ki-Soo;Lee, Jung-Han;Bae, Dong-Won;Kim, Dong-Kil;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.21 no.2
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    • pp.167-171
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    • 2005
  • We isolated the Cucumber green mottle mosaic virus(CGMMV) particles from watermelon leaves and designated as CGMMV-HY1 as a watermelon isolate and attempted to characterize the pathogenic isolate responsible for such an epidemic in watermelon and also to monitor dominant viral isolates in greenhouse. The watermelon plants infected with CGMMV generally showed mottle mosaic, mosaic, growth stunting, necrosis and deformed fruit. The reactions of indicator plants to CGMMV-HY1 were the local lesions on Nicotiana tabacum cv. White Burley, Nicotiana tabacum cv. Samsun, and Chenopodium amaranticola, and the mosaic symptoms only on Cucumis sativus, but the CGMMV-HY1 did not infect Nicotiana sylvesytis, Datura stramonium, Chenopodium quinoa, and Petunia hybrida. Purified virus particles were rod-shaped and about 300 nm long. The coat protein (CP) of purified CGMMV-HY1 was single band with molecular weight of about 16.5 kDa which was confirmed by western blot analysis probed with monoclonal antibody of CGMMV-HY1. The genomic and subgenomic RNAs of 6.4 kb and 0.75 kb were revealed by the electrophoresis on 1.2% formaldehydedenatured agarose gel. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequences. A 464bp of CP gene of CGMMV-HY1 was amplified by RT-PCR and cloned into PGEM-T easy vector. The nucleotide sequence of CP gene of CGMMV-HY1 shared 98%, 99%, and 100% identities with that of CGMMV strains W, KOM, and KW respectively. Based on these results, we identified CGMMV-HY1 as a CGMMV isolate of watermelon, a member of Tobamovirus.

A Study on the Traditional Sash of‘She’Ethnic Group in China (중국 소수민족 이족의 채대)

  • 김성희
    • Journal of the Korean Society of Costume
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    • v.39
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    • pp.59-77
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    • 1998
  • This paper is focused on the traditional sash weaving handicraft of‘She’ethnic group, which is located in Fujian, Zhejiang, Jiangxi, Guangdong province of China. This research is main-ly based on the field work, analyzed and inter-preted the traditional sash in systematic and reasoned way. The summary of this study are as follows : 1. On its technological aspect, weaving structure of the traditional sash is made of warp rod backed weaving. The used tool is primitive one but the weaving process includes scientific method. 2. From the social-cultural point of view, the sash ha been the symbol of love towards her lover. Every woman of this group had taken training for this sash weaving from a child. 3. On its ethnological aspect, it has been long history and has interchanged with other ethnic group like Miao, Han and also Okinawa country of Japan. The pattern inside this sash are almost looks like characters, but they are not Chinese characters whereas are the inde-pendent code of‘She’ group and have been inherent from ancestors and which will be tran-smitted to their posterity. These independent code of‘She’group are the traditional message to their later generation implicating their natural circumstances, human relationship, ethnic myth, spirit etc. 4. I recognize that the pattern inside the sash is defined as the communicative code and in comparison to language, it is more repetition and less apparent as close code. Nowadays China has been developed es-pecially in the economical fields rapidly. Under the circumstances traditional weaving culture of ethnic groups has been facing a crisis of disappearance, which will be a great loss for the country as well as the human beings. For this reason, I emphasize that it is very immediate to make co-researches into the material culture of Chinese ethnic groups.

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Isolation and Characterization of Novel Denitrifying Bacterium Geobacillus sp. SG-01 Strain from Wood Chips Composted with Swine Manure

  • Yang, Seung-Hak;Cho, Jin-Kook;Lee, Soon-Youl;Abanto, Oliver D.;Kim, Soo-Ki;Ghosh, Chiranjit;Lim, Joung-Soo;Hwang, Seong-Gu
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.11
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    • pp.1651-1658
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    • 2013
  • Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollutants from wastewater under thermophilic conditions. We therefore isolated thermophilic facultative bacterial strains from wood chips that had been composted with swine manure under aerated high temperature conditions so as to identify strains with denitrifying ability. Nine different colonies were screened and 3 long rod-shaped bacterial strains designated as SG-01, SG-02, and SG-03 were selected. The strain SG-01 could be differentiated from SG-02 and SG-03 on the basis of the method that it used for sugar utilization. The 16S rRNA genes of this strain also had high sequence similarity with Geobacillus thermodenitrificans $465^T$ (99.6%). The optimal growth temperatures ($55^{\circ}C$), pH values (pH 7.0), and NaCl concentrations (1%) required for the growth of strain SG-01 were established. This strain reduced 1.18 mM nitrate and 1.45 mM nitrite in LB broth after 48 h of incubation. These results suggest that the G. thermodenitrificans SG-01 strain may be useful in the removal of nitrates and nitrites from wastewater generated as a result of livestock farming.

Detection, Characterization and Antibiotic Susceptibility of Clostridioides (Clostridium) difficile in Meat Products

  • Muratoglu, Karlo;Akkaya, Esra;Hampikyan, Hamparsun;Bingol, Enver Baris;Cetin, Omer;Colak, Hilal
    • Food Science of Animal Resources
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    • v.40 no.4
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    • pp.578-587
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    • 2020
  • Clostridioides (Clostridium) difficile is a Gram (+), anaerobic, spore forming, rod shaped bacterium that can produce toxin. The objective of this study is to reveal the presence of C. difficile in meat products, to analyze the ribotype diversity by PCR and to evaluate the antibiotic susceptibility of isolated strains. The organism was isolated in 22 out of 319 (6.9%) examined meat product samples and 9 out of 22 (40.9%) isolates were identified as RT027 and all isolates had the ability of toxin production. In terms of antibiotic susceptibility, all isolates were susceptive to amoxicillin-clavulanic acid, tetracycline and vancomycin and 21 (95.4%) isolates to metronidazole. On the other hand, imipenem and cefotaxim resistance was observed in all. In conclusion, the results of this comprehensive study conducted in Turkey deduced the presence of C. difficile in different meat products. Therefore, these products can be evaluated as a potential contamination source of C. difficile from animals to humans especially for elders, youngsters, long terms wide spectrum antibiotic used and immuno-suppressed individuals.

Fretting Wear Characteristics of the Corroded Fuel Cladding Tubes for Nuclear Fuel Rod against Supporting Girds (부식된 핵연료 피복관과 지지격자 사이의 프레팅 마멸 특성)

  • Kim, Jin-Seon;Park, Se-Min;Kim, Yong-Hwan;Lee, Seung-Jae;Lee, Young-Ze
    • Tribology and Lubricants
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    • v.23 no.3
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    • pp.130-133
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    • 2007
  • Fuel cladding tubes in nuclear fuel assembly are held up by supporting grids because the tubes are long and slender. Fluid flows of high-pressure and high-temperature in the tubes cause oscillating motions between tubes and supports. This is called as FIV (flow induced vibration), which causes fretting wear in contact parts of tube and support. The fretting wear of tube and support can threaten the safety of nuclear power plant. Therefore, a research about the fretting wear characteristics of tube-support is required. The fretting wear tests were performed with supporting grids and cladding tubes, especially after corrosion treatment on tubes, in water. The tests were done using various applied loads with fixed amplitude. From the results of fretting tests, the wear amounts of tube materials can be predictable by obtaining the wear coefficient using the work rate model. Due to stick phenomena the wear depth was changed as increasing load and temperature. The maximum wear depth was decreased as increasing the water temperatures. At high temperatures there are the regions of some severe adhesion due to stick phenomena.

Fretting Wear Characteristics of the Corroded Fuel Cladding Tubes for Nuclear Fuel Rod against Supporting Girds (부식된 핵연료 피복관과 지지격자 사이의 프레팅 마멸 특성)

  • Lee, Young-Ze;Kim, Jin-Seon;Park, Se-Min;Kim, Yong-Hwan;Lee, Seung-Jae
    • Tribology and Lubricants
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    • v.24 no.3
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    • pp.129-132
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    • 2008
  • Fuel cladding tubes in nuclear fuel assembly are held up by supporting grids because the tubes are long and slender. Fluid flows of high-pressure and high-temperature in the tubes cause oscillating motions between tubes and supports. This is called as FIV (flow induced vibration), which causes fretting wear in contact parts of tube and support. The fretting wear of tube and support can threaten the safety of nuclear power plant. Therefore, a research about the fretting wear characteristics of tube-support is required. The fretting wear tests were performed with supporting grids and cladding tubes, especially after corrosion treatment on tubes, in water. The tests were done using various applied loads with fixed amplitude. From the results of fretting tests, the wear amounts of tube materials can be predictable by obtaining the wear coefficient using the work rate model. Due to stick phenomena the wear depth was changed as increasing load and temperature. The maximum wear depth was decreased as increasing the water temperatures. At high temperatures there are the regions of some severe adhesion due to stick phenomena.

Electrochemical Properties of Carbon/Manganese Oxide Composite Air Cathode for Lithium-Air Batteries (리튬-공기전지용 탄소/망간산화물 복합구조 공기극의 전기화학적 특성)

  • Lee, Sun-Young;Cha, Eun-Hee;Mho, Sun-Il;Ju, Jeh-Beck;Cho, Won-Il
    • Journal of the Korean Electrochemical Society
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    • v.15 no.3
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    • pp.198-205
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    • 2012
  • Carbon-supported manganese oxide composite were fabricated as an air cathode material for Li-air batteries by hydrothermal method. The composite materials of carbon and manganese oxide were investigated by the implementation of X-ray diffraction, FE-SEM and BET surface area measurer. The manganese oxide synthesized at $170^{\circ}C$ for 12 h has a rod like shape morphology with 40-50 nm long in size. A Lithium-air battery with coin type, of which electrodes are composed of cathode composite materials synthesized $170^{\circ}C$-12 h and lithium metal anode, reveals its first discharge capacity of 3,852 mAh/g and four discharge-charge cycles.

Preparation of Co3O4/NF Anode for Lithium-ion Batteries

  • Tian, Shiyi;Li, Botao;Zhang, Bochao;Wang, Yang;Yang, Xu;Ye, Han;Xia, Zhijie;Zheng, Guoxu
    • Journal of Electrochemical Science and Technology
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    • v.11 no.4
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    • pp.384-391
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    • 2020
  • Due to its characteristics of light weight, high energy density, good safety, long service life, no memory effect, and environmental friendliness, lithium-ion batteries (LIBs) are widely used in various portable electronic products. The capacity and performance of LIBs largely depend on the performance of electrode materials. Therefore, the development of better positive and negative materials is the focus of current research. The application of metal organic framework materials (MOFs) derivatives in energy storage has attracted much attention and research. Using MOFs as precursors, porous metal oxides and porous carbon materials with controllable structure can be obtained. In this paper, rod-shaped Co-MOF-74 was grown on Ni Foam (NF) by hydrothermal method, and then Co-MOF-74/NF precursor was heat-treated to obtain rodshaped Co3O4/NF. Ni Foam was skeleton structured, which effectively relieved. The change of internal stress changes and destroys the structural volume of the electrode material and reduces the capacity attenuation. Co3O4/NF composite material has a specific discharge capacity of up to 1858 mA h/g for the first time, and a reversible capacity of up to 902.4 mA h/g at a current density of 200 mA/g, and has excellent rate and impedance performance. The synthesis strategy reported in this article opens the way to design high-performance electrodes for energy storage and electrochemical catalysis.

Shape Control of Anodic Aluminum Oxide and Effect as Support of Silicon Powder Electrode (양극산화알루미늄의 형상제어와 이를 이용한 실리콘 분말 전극 지지체 효과)

  • Song, Ju-Seok;Ha, Jong-Keun;Kim, Yoo-Young;Park, Dong-Kyu;Ahn, In-Shup;Ahn, Jou-Hyeon;Cho, Kwon-Koo
    • Journal of Powder Materials
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    • v.22 no.4
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    • pp.240-246
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    • 2015
  • Anodic aluminum oxide (AAO) has been widely used for the development and fabrication of nano-powder with various morphologies such as particle, wire, rod, and tube. So far, many researchers have reported about shape control and fabrication of AAO films. However, they have reported on the shape control with different diameter and length of anodic aluminum oxide mainly. We present a combined mild-hard (or hard-mild) anodization to prepare shape-controlled AAO films. Two main parameters which are combination mild-hard (or hard-mild) anodization and run-time of voltage control are applied in this work. The voltages of mild and hard anodization are respectively 40 and 80 V. Anodization was conducted on the aluminum sheet in 0.3 mole oxalic acid at $4^{\circ}C$. AAO films with morphologies of varying interpore distance, branch-shaped pore, diameter-modulated pore and long funnel-shaped pore were fabricated. Those shapes will be able to apply to fabricate novel nano-materials with potential application which is especially a support to prevent volume expansion of inserted active materials, such as metal silicon or tin powder, in lithium ion battery. The silicon powder electrode using an AAO as a support shows outstanding cycle performance as 1003 mAh/g up to 200 cycles.

The Implemention of RTD-l000A based on ARM Microcontroller (ARM 마이크로컨트롤러 기반 RTD-1000A의 구현)

  • Kim, Min-Ho;Hong, In-Sik
    • Journal of Internet Computing and Services
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    • v.9 no.6
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    • pp.117-125
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    • 2008
  • With increase of concern about the Ubiquitous application, the necessity of the computer system which is miniaturized is becoming larger. The ARM processor is showing a high share from embedded system market. In this paper, ideal method for RTD-1000 controller construction and development is described using ARM microcontroller. Existing RTD-1000 measures distance of disconnection or defect of sensing casket by measuring receiving reflected wave which was sent via copper wire inside the leaking sensing rod. Using this RTD-1000, leakage and breakage of water and oil pipe can be sensed and it reports damage results to the networks. But, existing RTD-1000 wastes hardware resources much and costs a great deal to installation. Also, it needs a cooling device because the heating problem, and has some problem of the secondary memory unit such as the hard disk. So, long tenn maintenance has some problems in the outside install place. In this paper, for the resolving the problem of RTD-1000, RTD-1000A embedded system based on ARM is proposed and simulated.

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