• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1342-1351
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• 2008

The feasibility of the use of Flp-mediated cassette exchange in the development of a CHO cell line, which produces erythropoietin (EPO) stably and largely, was investigated. A stable, high enhanced green fluorescence protein (EGFP)-producing clone was screened by extensive flow cytometric analysis. An EPO expression unit was targeted into the premarked locus of the stable parental clone by Flp-mediated cassette exchange and a correctly targeted clone (FC28T7) was obtained. The EPO production of FC28T7 was proven to be stable in long-term culture. Furthermore, the Flp-mediated cassette exchange did not alter the stable parental clone's characteristics concerning transgene expression level and stability. Taken together, the data obtained here indicated that the establishment of CHO cell lines stably producing a desired protein is achievable using Flp-mediated cassette exchange.

• 복식문화연구
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• 제1권2호
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• pp.145-157
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• 1993

This study aimes to investigate the garment ease of pe-school children's clothing in accordance with arm movement. The experiment was done with 4 per-school children fro mage 3 to age 5, and 3 types of experimental clothes were made ; sleeveless, half sleeve, and long sleeve. The waist pattern and the sleeve pattern of each experimental clothes has no garment ease. And experimental clothes were examined to obtain the necessary ease of armcye line and waist lien by cross-cut method. The results of investigation can be summarized as follows ; 1. The resulting movement ranges of experimental clothes with no garment ease were 131.5°(sleeveless), 71.75°(half sleeve), and 62.25°(long sleeve). 2. The size order of cross-cut opening of waist lien movement were (side>front>back), and (half sleeve>long sleeve>sleeveless). 3. When the base point of measurement was set to armpit point by arm the latitudinal length of cross-cut opening of armcye line by arm movement was (anterior armpit part > posterior armpit part), and longitudinal length of cross-cut opening was (upper part>lower part).

• Asian-Australasian Journal of Animal Sciences
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• 제20권7호
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• pp.1015-1022
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• 2007

The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be realized if stem cells could be cultured, transfected with unique genes, and then transplanted into recipient testes. We developed a culture system that supported long-term viability of SSCs. We used a retrovirus vector (pMSCV including ${\beta}$-galactosidase) to stably transfect spermatogonia following long-term culture using the system developed. Expression of the reporter gene ${\beta}$-galactosidase controlled by the retroviral vector was stable in long-term cultured SSCs. We confirmed the retroviral-mediated ${\beta}$-galactsidase gene could be expressed in germ cells in recipient mice following SSCs transplantation.

• 통상정보연구
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• 제11권1호
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• pp.67-92
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• 2009

This study investigates the relationship between Culture and online trust in three cultural dimensions: individualism, collectivism and long term orientation. For this purpose, this study proposes the empirical hypothesis to test the implication of the proposed model. The results are as follows. First, the purchase intention is influenced by the online trust in statistical sense. Second, the relationship between Culture and the antecedents of online trust are statistically established in three cultural dimensions: individualism, collectivism and long term orientation. The direction of future research is to develop the more refined hypothesis to test the empirical model in the field of on line trust and culture.

• 복식문화연구
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• 제6권4호
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• pp.13-25
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• 1998

This study focuses on the visual effects of various neckline-haristyle combinations on the perception of face image and type. The study employed a model with oval face and examined 35 combinations made up of five necklines and seven hairstyles. Looking at various face images depending upon different necklines, in case of round, V, boat square, and high necklines, long wave hair and medium wave hair produced a feminine image of marked individuality; long straight hair that covers the forehead and medium straight hair gave an image of charm and purity as well as an image of neatness; and long straight hair short cut hair showed an intellectual image. Regarding the perceptual type of face depending upon the different necklines of round, V, square, and high, the long straight hair covering the forehead and medium wave hair had the effect of an optical illusion that made the face look short and round; and short cut hair made the face line look distinct and the face look oval and slender.

• 복식문화연구
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• 제4권3호
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• pp.373-381
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• 1996

Draping design system organized by European about 13C has been developed greatly for a long while, but for the purpose of the practical use the Dress form similar to human body has been needed. In order to make three-dimensional effect as fitting Muslin ot the Dress form. the Basic lien has to be established in Muslin and Dress form each. At this time, Shoulder-blade level is indicated from various angles : measure down 10cm from the back neck point, a quarter of the back neck point to the waist lie, the half-way point between neckline and bust line, measure down 41 inches from the back neck line, measure down 3 inches from the top edge of the muslin at center back and cross mark for neck line, and so forth. This study established the Basic line through the two ways of them (B.N.P∼B.L/2, B.N.P∼W.L/4), did tight fitting to the 5 kinds of Dress forms which ar normal type in the drop value(the difference between hip circumference and bust circumference), and acquired Basic Pattern. After the experiment, fitness are throughly investigate by statistical analysis of measurements. As a result, this study finds out that fit is proper when shoulder blade level is situated on a fourth of the back neck point to the waist line and a quarter point between back neck point and waist line, and waist dart is situated on the back center line and princess line.

• 복식문화연구
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• 제10권4호
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• pp.319-335
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• 2002

Nongae was the official Kinye(관기) who had exhisted, her portrait was enshrined at an ancestral tablet hall beside Choksukloo. This portrait of Nongae was a work of a painter, Un Ho-Kim. In any case, a study on the costume of the portrait of one person must be taken the costume of the period. This study investigates the character of clothing style in King Sunjo 26(1593) and in her present portrait. And so, her portrait who was enshrined at the place didn't agree with it's clothing in King Sunjo 26(1593) The results of this study are as follows : The portrait of Nongae took the costume style of the beginning of about 1910. and so this style was different from the style of costume, King Sunjo 26(1593). The style of costume, King Sunjo 26(1593) : Jacket : It's jacket was long(near hip) and broad, it's sleeve length was long, it's form was a straight line. Skirt : It's skirt was very long. It's silhouette was formed a soft line The costume of the figure in this picture in wrong from historical point of view. And so, the costume of Nongae in the portrait must adjust it in the clothing style of about 1593.

• 한국패류학회지
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• 제30권3호
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• pp.273-279
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• 2014

The mass mortalities have been occurring of Korean scallop Patinopecten yessoensis from 1997's to now in Korea east sea. Cages behavior and interference effect (common name; curtain effect) between scallop cages were investigated in culture grounds on the eastern coastal waters of Korea for understand to mechanism of rising about mass mortalities of Korean scallop quickly. The first experiment was carried out in circulating water channel to assess inclination angel from relationship between velocity and cages interval, velocity with culture cages position. An angle of inclination of scallop culture cages were 94.6 to 92.3 degree under a several velocity which were from 0.1 m/s and 131.9 to 118.1 degree under 0.5 m/s with cages interval were 1 m, 94.3 to 91.0 degree under velocity is 0.1 m/s and 133.2 to 122.4 degree under 0.5 m/s with cages interval were 1.5 m and 94.6 to 96.4 degree under velocity is 0.1 m/s and 131.7 to 131.8 under 0.5 m/s with cages interval were 2 m. The second experiment was designed to prove the tank test. Velocities were measured inside and outside of the scallop culture ground at eastern sea of Korea. The velocity of inside of the culture was the slowest as 0.1m/s. In this result, interference between former cage and after cage was occurred.

• 복식문화연구
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• 제14권4호
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• pp.609-624
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• 2006

The purpose of this study is to give the guideline to the pattern designing by supplying various images of the transformed shape of tailored collar with the location and angle of gorge line and laying measurement of the tailored collar while designing the tailored collar. The results of this study are as follows : Images of the sample at the case of 1cm of laying measurement looks 'urban', 'light', 'sharp', 'young', 'fashionable' and 'narrow' than that at the case of 7cm. When the laying measurement increases, the image of the sample looks more 'classical' and 'boorish'. The image of the sample 'light', 'sensuous' and 'young' at the case of sample of which laying measurements of the collar was 3cm. Second, at the case of sample whose laying measurements of the collar was 5cm, it was shown that the low location of gorge line made the image of the sample 'soft' and 'short'. On the other hand the image of the sample looked 'hard', 'keen' and 'long' by the high location of gorge line. Third, at the case of sample of which laying measurements of the collar was 7cm, low location of gorge line made the image of the sample look short. However, normal location of gorge line made the image of the sample looked 'fashionable'. The images of the samples of which laying measurements of the collar were 3cm, 5cm and 7cm, respectively, had changed with the angle of gorge line. First, the high angle of gorge line made the image of the sample 'rigid' and 'keen' at the case of sample of which laying measurements of the collar was 3cm. The high angle of gorge line made the image of the sample looked 'boorish' and 'singular'.

• 복식문화연구
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• 제8권6호
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• pp.915-929
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• 2000

As a result of research, the character of the general dress-costumes from the late 17th century to the early 18th century in Genre Paintings of DuSeo Yun and YoungSeok Cho is as follows. Firstly young women wore braided hair at the back of head and married women wore hair in the style of Unjeun-mori with Gache. The length of Jeogori (Korean traditional jacket) covered waistor shortened to waist length. The width of Jeongori was suitable, because side-seam line was straight or oblique as it comes into inner line. The width of Git was enough and Mokpan-git (shape of board) and Kal-kit (shape of knife) appeared, and sometimes used other color fabric. Sleeve was narrow and ostly folded up. Chima (Korean traditional skirt) used darker color fabric than Jeogori and the width of Chima was narrow and its length was short. Sokbaji (Korean traditional underpants) shown below were narrow and its end narrower. They were barefoot or they wore Hye (Korean traditional shoes) after putting on Beoseon (Korean traditional socks). Secondly general man's hair tie a topknot (sangtu) and put on headdres, 'Bang-lip', 'Mang-geon', hairband, 'Tang-geon' on head. The length of Jeogori became shorter from the line which covered hip to the line which covered waist. The width of Jeogori was suitable and sometimes it had a slit of side-seam line. The width of Git (neckand) was wide and the length of Git was long. 'Kal-git'appeared and it used other color fabric. The shape of sleeve was straight and narrow. They folded up their sleeves. They folded up their sleeves. They folded up their slack that look like 'Jam-bang-i'and the width of slacks was not suitable, and it was narrow. Baji (Korean traditional pants) were with or without knot, worn 'Hangjeon'(ankle band). They were barefoot and wore 'Hye'or 'Jipsin'(Korean traditional straw shoes). Thirdly a person of high birth or a low-ranked official put 'Yu-geon', 'Mang-geon', 'Gat', 'Tang-geon', 'Bok-du', 'Bok-geon', 'Whi-hang'on their head on a topknot. They wore 'Po (Shim-ui, Jick-ryeong'Jung-chi-mak, Do-po, etc)'on Baji and Jeogori. 'Po'was long and wide, it knot with 'Se-jo-dae'(string belt) or 'Po-baek-dae'(band belt). It had a slit of sideline and 'Mu'which had or had not or which were hard to confirm. The shape of sleeve was straight or very wide and its length was long. The width of Baji was wide and knotted with 'Hangjeon'and wore'Beoseon'and 'Hye'. Fourthly child's hair was short or knotted to the back of the head. The length of Jeogori reached waist line and its width was wide. It had a 'Jeogori'which had s slit of sideline. The shape of sleeve was 'Tong-su'(straight), and the length of sleeve was diverse. They put 'Baeja'on 'Jeogori'. The width of Baji was not wide. They wore them straight without or with knot, 'Hangjeon'. They were barefoot or put on 'Jipsin'.