• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.243-250
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• 2004

In an effort to optimize tissue culture responses of Italian ryegrass(Lolium multiflorum Lam.) for future genetic manipulations to improve forage characteristics, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of three cultivars, 'Jeanne', 'Florida-80' and 'Metro', as explant tissues. For all explants, MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The optimal concentration of dicamba for the induction of embryogenic callus from mature seeds was 7mg/L. The highest plant regeneration frequency was observed when embryogenic callus was transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Plant regeneration frequency of callus cultured in the dark was higher than that of cultured in the light. Casein hydrolysate and L-proline improved both in embryogenic callus induction from mature seeds and plant regeneration. High-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.525-530
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• 1999

Prior to ensiling Rhodesgrass (RG) and Italian ryegrass (lRG) were treated with lactic acid bacteria (LAB) or with LAB+cellulases to compare their fermentation characteristics and chemical compositions. LAB (Lactobacillus casei) was added to all ensiling materials (except the untreated control) of RG and IRG at a concentration of $1.0{\times}10^5\;cfu.g^{-1}$ fresh forage. The enzymes used were Acremoniumcellulase (A), Meicelase (M) or a mixture of both (AM). Each enzyme was applied at levels of 0.005, 0.01 and 0.02 % of fresh forage. The silages with each treatment were incubated at 20, 30 and $40^{\circ}C$ and stored for about 2 months. While no marked differences were found between the RG and IRG silages with various treatments on dry matter (DM), volatile basic nitrogen (VBN) and water soluble carbohydrate (WSC) contents, there were significant differences in pH value, and lactic acid and butyric acid contents. LAB inoculation did not affect the fermentation characteristics of either the RG or IRG silages. The combined treatments of LAB+cellulases improved the fermentation quality of both the RG and IRG silages as evidenced by the decrease in pH value and increase in lactic acid content. Increasing the amount of added cellulase resulted in a decrease in pH value and an increase in lactic acid content in both the RG and IRG silages. Cellulases A and AM had a greater effect than cellulase M on the fermentation quality of the RG and IRG silages. Incubation temperatures of 30 and $40^{\circ}C$ appeared to be more appropriate environments for stimulating good fermentation than $20^{\circ}C$.

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.4
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• pp.265-274
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• 2000

To investigate the effects of anion replacement on NO3- concentration in Italian ryegrass. Plants weregrown hydroponically to the full vegetative stage. NO3 supply(control) was replaced with SO1- (Tl), C1-(T2) and water (T3) to during 14 days. The determination of inorganic nutrient uptake and quantification ofprincipal metabolites (nitrate. protein and sugar) followed. Relatively high uptake of ~ a a'n d Ca" for controlplants, K+ and POJ- for T2 plants, and C 1 for TI plants was observed, respectively. Proteins in shoot andstubble were relatively higher in control and TI plants, which coupled N source. In root proteins largelydecreased (especially in T3 plants) during experimental period. Sugars in shoot of all four treatments tendedto decrease during the first 7 days and recovered afterward. Sugars in stubble also markedly decreased duringthe first 7 days, while those in root was much less varied during experimental period. After 14 days oftreatment, nitrate concentration in shoot of control plants was 13mgIg FW. Comparing to control: nitrate inshoot reduced by 27%, 46% and 50% in TI, T2 and T3 plants, respectively. Dry weight was slightlyincreased or not significantly changed in control, T1 and T2 plants, while a significant decrease(31.3% ofcontrol) occurred in T3 plants.(Key words : Italian ryegrass, Anion replacement, Ion uptake, Protein, Sugar, Nitrate)ptake, Protein, Sugar, Nitrate)

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.4
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• pp.222-228
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• 2022

This study was conducted to investigate the effect of waterlogging stress on the physiological response and growth characteristics of the five Italian ryegrass varieties. For all varieties, the germination rate of seed decreased by 10-15% as the waterlogging period increased, but the waterlogging stress treatment after the early seedling stage increased the growth of shoot and root length. Photosynthetic activity (Fv/Fm) according to waterlogging stress treatment decreased in all vareity, and Florida 80 showed the least decrease with 1.5%. Waterlogging stress treatment was found to reduce the accumulation of reactive oxygen species (malondialdehyde, MDA) and the activities of antioxidant enzymes. These results suggest that other mechanisms may be involved in the defense mechanism of Italian ryegrass against waterlogging stress, such as promoting root growth to escape from waterlogging stress, in addition to the antioxidant enzyme system.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.265-272
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• 2002

Forage breeding laboratory of National Livestock Research Institute, R.D.A. has made interspecific hybrids of Lolium multiflorum $\times$ L. pratensis and intergeneric hybrids of Lolium $\times$ Festuca since 1984, and has collected ecotypes of Italian ryegrass since 1991. Growth characteristics of these hybrids and ecotypes were researched, and then these clone lines were named. Among these clone lines, the several clones that have polen fertility, high cold-tolerance, and similar heading time were used for making synthetics, Naehan 6, 7, 8, 9, with polycrossing method in 1997. Field experiments were carried out to compare the mophological and agronomical characteristics and forage productivity and quality of the synthetics with those of Italian ryegrass varieties, Barmultra and Hwasan 101. in Suwon and Yonchun from 1999 to 2000. Heading time of the synthetics were 22th to 24th May that belong to late-mature types to be similar to that of Barmultra and Hwasan 101 in Suwon. The synthetics were 101 to 106 c3n in plant length, medium or thick in thickness of stem, dark peen in leaf color, broad and long in flag leaf, strong in lodging resistance, and excellent in regrowth. Winter survivals of the synthetics were no different from that of Barmultra or Hwasan 101 in Suwon, but better than that of Barmultra or Hwasan 101 in Yonchun where was -10 to -12$^{\circ}C$ of minimum average air temperature in January or February. Dry matter(DM) yields of the synthetics were similar to DM 8,238kg per ha of Barmultra in Suwon, but in Yonchun, were more 7 to 13% than DM 7,291kg per ha of Barmultra. Forage qualities, IVDMD, ADF, NDF and TDN of the synthetics were lower than those of Hwasan 101, but higher than those of Barmultra.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.1
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• pp.27-35
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• 1990

As a part of studies on potassium(K) behavior in grassland with respect to magnesium(Mg) balance of ruminants, seasonal variation of K and Mg contents of forages including native gasses grown in grazing pasture and meadow were investigated. During an experimental period from April to October of 1984, two times of grazings were carried out in the orchardgrass (Dacfylis glomerata L.) and the tall fescue (Festuca arundinacea Schreb.)dominant grazing pastures, and forage plants (forages and native grasses) were sampled monthly and also K and Mg contents were determined without separating into individual plant species (Experiment 1). All the plant species grown in the two meadows which situated in the grazing pastures were harvested five times during the same period, separated into individual plant species, and botanical composition (SDR, ) and K and Mg contents of the plant species were determined (Experiment 2). The results obtained were as follows: 1. During the experimental period in the orchardgrass grazing pasture K contents of the forage plants were the highest in spring, and the seasonal variation of the contents in the orchardgrass pasture (1.5-5.8 % in a dry matter basis) was more significant than that of forage plants in the tall fescue grazing pasture (3.0- 3.8 %). 2. The Mg contents of forage plants in the orchardgrass grazing pasture ranged under 2.0 mg/g DW from Arpil until July, and the contents in the orchardgrass pasture (1.5-3.1 mg/g DW) was in the lower range than that of forage plants in the tall fescue pasture (2.0-3.8 mg/g DW). (Experiment I). 3. Orchardgrass was the dominant species in the orchardgrass meadow until July, but several species of native grasses were observed from summer (July) and native grasses such as Digitaria adscendens and Echinochlw crus-galli became dominant in autumn (October). 4. Seasonal variation of K contents of orchardgrass was in the range of 3.9-5.9 %, and the contents was higher in spring (May) and in autumn (October). The variation of white clover (Trifolium repens L.) was in the range of 3.6-5.0 %, that of tall fescue 3.8-4.8 %, and that of Italian ryegrass (Lolium multiflorum Lam.) 2.7-3.5 %, respectively. 5 . Seasonal variation of Mg content of white clover was in the range of 2.9-3.7 mg, that of tall fescue 2.0- 3.3 mg, and that of orchardgrass 1.6-2.8 mg/g DW, respectively. The variation of the contents of Italian ryegrass was in the range of 1.3-1.9 mg/g DW. And Mg contents of the forage plants were higher in summer(July) 6. In autumn (October and November ) native grasses such as D. adscendens and E. crus-galli contained lower K contents (1.7-3.9 %), but higher Mg contents (3.2-10.1 mg/g DW) than the forages contained. (Experiment 2) From the results above, it is known that K contents ranged higher in younger forages in viewpoint of growth stage and higher in spring and autumn, and that Mg contents ranged lower in spring. Therefore, the mineral imbalance or hypomagnesaemic (grass) tetany can be triggered in spring or autumn, and more frequently by such plant species as orchardgrass and Italian ryegrass with lower Mg and/or higher K contents than by tall fescue. And it is suggested that the dominant native grasses in autumn such as D. adscendens and E. emsgalli can contribute to the prevention of the tetany with higher Mg and lower K contents.

• Journal of the Korean Institute of Landscape Architecture
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• v.18 no.2
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• pp.45-55
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• 1990

This study describes on the erosion control effects of the several grasses and its mixtures for the man-made slopes. The grasses used for this experiment include cool-season grasses such as Festuca rubra L. (Creeping redfescue), Poa pratensis L. (Kentucky bluegrass), Lolium perenne L. (Perenial ryegrass), Lolium multiflorum LAM. (Italian ryegrass), Festuca arundinacea Schrel. (Tall fescue), and warm-season grasses such as Eragrostis curvula Schrad. (Weeping lovegrass), Zoysia japonica Steud. (Zoysiass) and native plants (Artemisia princeps var. orientalis Hara, Lespedeza cuneata G. Don, Arundinella hirta var. ciliata K.) This study was conducted at Dan-kook University from April, 1988 to Octover, 1989. The results are summurized as follows; 1.Cool-season grasses covered the ground quickly in early stage, and weekened slowly during sumer season. Warm-season grasses and native-plants covered the ground slowly in early stage, but during summer season they grew vigorously, so outweighed cool season grasses. 2. The amount of aboveground growth of weeping locearass and underground growth of Artemisia prinoepts are quite differant from others. Since Arumdinella hirta has deep root system, it is thought to very useful protection of unstable for hrdro-seeding. Because cool-season grasses are useful for quick coverage, and native plants or warm-season grow well during summer season with the better compatability to weeds. 3.Mixture III(cool-season and warm-season grasses), mixtureIV(native spp. and Italian ruegrass), and mixtureV(native spp.) resulted in better control of erosion control on man-made slopes. Native spp. has equivallent capacity of erosion control compared to several foreign grasses.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.277-284
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• 1998

This experiment was carried out to study the effects of lactic acid bacteria (LAB) inoculation and addition of cell wall degrading enzymes on the fermentation characteristics and chemical compositions of Italian ryegrass silage. An inoculant LAB with or without a cell wall degrading enzyme of Acremoniumcellulase (A), or Meicellulase (M) or a mixture of both (AM), was applied to 1 kg of fresh Italian ryegrass sample. The treatments were control untreated, LAB-treated (application rate $10^5$ cfu/g fresh sample), LAB+A 0.005%, LAB + A 0.01%, LAB+A 0.02%, LAB + M 0.005%, LAB + M 0.01%, LAB + M 0.02%, LAB+AM 0.005%, LAB + AM 0.01% and LAB+AM 0.02%. The sample was ensiled into 2-L vinyl bottle silo, with 9 silages of each treatment were made (a total of 99 silages). Three silages of each treatment were incubated at 20, 30 and $40{^{\circ}C}$ for an approximately 2-months storage period. All silages were well preserved as evidenced by their low pH values (3.79-4.20) and high lactic acid concentrations (7.71-11.34% DM). The fermentation quality and chemical composition of the control untreated and the LAB-treated silages were similar, except that for volatile basic nitrogen (VBN) content was lower (p < 0.05) in the LAB-treated silages. LAB + cellulase treatments improved the fermentation quality of silages by decreasing (p < 0.01) pH values and increasing (p<0.01) lactic acid concentrations, in all of cellulase types and incubation temperatures. Increasing amount of cellulase addition resulted in further decrease (p < 0.01) of pH value and increases (p < 0.01) of lactic acid and residual water soluble carbohydrate (WSC) concentrations. LAB + cellulase treatments reduced (p<0.01) NDF, ADF, hemicellulose and cellulose contents of silages compared with both the control untreated and LAB-treated silages. LAB + cellulase treatments did not affect the silage digestibility due to fact of in vitro dry matter digestibility (IVDMD) was similar in all silages. The silages treated with cellulase A resulted in a better fermentation quality and a higher rate of cell wall reduction losses than those of the silages treated with cellulases M and AM. Incubation temperature of $30{^{\circ}C}$ seemed to be more suitable for the fermentation of Italian ryegrass silages than those of 20 and $40{^{\circ}C}$.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.351-357
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• 2011

An experiment was conducted to evaluate the effects of castration on growth performance, carcass characteristics, and meat quality of goat kids. Fourteen Boer-cross buck and wether goat kids (n = 7; initial body weight (BW) $38.0{\pm}0.35\;kg$ and $34.8{\pm}0.35\;kg$, for bucks and wethers, respectively) were grazed on annual Marshall ryegrass (Lolium multiflorum Lam.) for 56 days. Body weights were recorded after 4 h withdrawal from feed and water for two consecutive days, every 2 wk. After d 56, animals were harvested and hot carcass weight (HCW), cold carcass weight (CCW), dressing percent (DP), kidney and pelvic fat (KPF), longissimus muscle (LM) area, back fat (BF), and other carcass parameters were measured. Day 0 BW was used as a covariate for analyses. However, bucks were heavier than wethers at d 15 (p = 0.09), 42 (p = 0.001) and 56 (p = 0.001). Bucks had higher ADG (146 vs. 74 g/d; p = 0.001), HCW (21.2 vs. 18.8 kg; p = 0.06) and CCW (20.3 vs. 17.9 kg; p = 0.04) when compared with wether goats. Dressing percentage (51 vs. 47%; p = 0.06), KPF (0.44 vs. 0.16%; p = 0.02) and BF (0.41 vs. 0.21 cm; p = 0.05) were higher in wethers vs bucks, respectively; however, USDA live or carcass grades were similar. Longissimus muscle tissue from wethers and bucks were similar in darkness ($L^*$) and redness ($a^*$), but wethers had more (p = 0.02) yellow tint ($b^*$). Palmitic (C16:0), stearic (C18:0) and oleic (C18:1) acids were higher (p = 0.001) in muscle tissue from wethers compared to bucks. The saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) contents of muscle tissue were lower (p = 0.001) for bucks with no difference in polyunsaturated fatty acids (PUFA). Longissimus muscle initial temperature was higher in bucks (p<0.04) and pH change post-mortem was similar for bucks and wethers. These results indicated that castration of young market goats reduced growth performance and produced carcasses with more fat and higher SFA.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.340-350
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• 1995

This study was conducted to obtain the basic information for the salt stress of Italian Ryegrass. Fourteen cultivars including 7 diploid and 7 tetraploid were subjected to eight levels of NaCl treatments during germination and early seedling stages. Germination percentage decreased remarkably over 300mM of NaCl concentration. The NaCl concentration that inhibited germination of 50% of the viable seeds in the cultivars was 344mM. The decreasing degree of germination percentage was higher in tetraploid type than in diploid type. The varietal difference of germination percentage was shown to be very conspicuous as the NaCl concentration increased. Top dry weight in 166 mM NaCl was decreased by 50% compared with that of control. Inhibition of NaCl appeared to be more in early seedling stage than in germination stage. Top dry weight of cultivars in NaCl stress was not related with germination capacity. Root dry weight in 148mM NaCl decreased by 50% compared with that of control. Root dry weight of cultivars was significantly correlated with top dry weight in NaCl treatment. Top dry weight and root dry weight was higher in diploid type than in tetraploid type at each 8 levels of NaCl concentration. Total chlorophyll content increased with the increase of NaCl concentration and the degree of increase in chlorophyll a was higher than that in chlorophyll b. Chlorophyll a was higher in tetraploid type than in diploid type. But chlorophyll b was higher in diploid type than in tetraploid type. There were significant correlation between chlorophyll a and top dry weight of cultivars over 150mM of NaCl concentration. Free proline content accumulated remarkably in NaCl treatment compared with control and was not difference between diploid type and tetraploid type. Free proline content of cultivars was not related with top dry weight in NaCl treatment.