• Animal cells and systems
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• v.3 no.2
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• pp.193-197
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• 1999

A peroxidase-antiperoxidase method was used to detect the cells showing immunoreactivities to six hormone antibodies in the alimentary tracts of six frog species, Rana nigromaculata, R. rugosa, R. amurensis coreana, R. catesbeiana, Bombina orientalis, and Hyla arborea japonica, inhabiting Korea. The cells immunoreactive to gastrin and cholecystokinin-8 were observed in the pylorus of the stomachs and in the small intestines of all frog species examined. In contrast, these somatostatin-immunoreactive cells were identified in the esophagus and the whole gastrointestinal tracts, but were absent from the large intestines in R. rugosa, R. catesbeiana, B. orientalis and H. arborea japonica. The pancreatic polypeptide (PP)-immunoreactive cells represented their distribution limited to the small intestines of R. amurensis coreana and H. arborea japonica, and they were additionally identified in the pylorus of the stomachs in the other four species. Serotonin- and glucagon- Immunoreactive cells revealed different regional distributions in which the former were observed throughout the whole alimentary tracts in all frog species investigated, whereas the latter were not found in these regions at all. Endocrine cells were relatively abundant in the pyloric portion of the stomach compared to other organs. The present study showed that all endocrine cells except for PP had a similar distribution in the alimentary tracts of all frog species used.

• Journal of The Korean Digital Architecture Interior Association
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• v.11 no.1
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• pp.33-42
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• 2011

As the development of construction technology and new materials, building requirements has been varied gadually. Comfortable environment and serviceability of production activity and energy conservation are being dealt with very seriously. Recently localization of engineering technology of Power Plant, however, construction materials and domestic technology are being developed forcingly. According to above topics this thes is going to study roof waterproofing, thermal insulation and evaluate adiabatic performance and evaluation of properties of waterproofing materials and energy conservation. The results of studying and evaluating of roof waterproofing, thermal insulation and adiabatic performance of Power Plant are as follows. 1. Sheet waterproofing method is better than that of asphalt waterproofing method in that adaptability of wearhertight, thermal resistant, contraction and expansion. 2. It is required to replace polyurethane or ethylene used as thermal insulation with rock wool which is noncombustible materials. 3. It is recommended to usd outer insulation method than inner insulation method due to superioty of outer insulation method. Efficiency of insulation materials used in power plant is generally good except perlite mortar used in the power plant(YGN 1-2, GRI 1-2).

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2033-2038
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• 2003

This paper describes an integrated navigation strategy for the autonomous service robot PSR. The PSR is under development at the KIST for service tasks in indoor public environments. The PSR is a multi-functional mobile-manipulator typed agent, which works in daily life. Major advantages of proposed navigation are as follows: 1) Structured control architecture for a systematic integration of various software modules. A Petri net based configuration design enables stable control flow of a robot. 2) A range sensor based generalized scheme of navigation. Any range sensor can be selectively applied using the proposed navigation scheme. 3) No need for modification of environments. (No use of artificial landmarks.) 4) Hybrid approaches combining reactive behavior as well as deliberative planner, and local grid maps as well as global topological maps. A presented experimental result shows that the proposed navigation scheme is useful for mobile service robot in practical applications.

• Journal of Korea Multimedia Society
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• v.21 no.5
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• pp.583-591
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• 2018

With the development of ICT(Information and Communication Technology), the number of smart devices is rapidly increasing. LBS(Location Based Service) applications that provide user's location based service are used in various fields. There is also a growing demand for indoor precision positioning technology to provide seamless services. In this paper, we propose an indoor positioning system that estimates the location of a smartphone user. The proposed algorithm determines whether the received signal is LOS(Line-of-Sight) or NLOS(Non-Line of-Sight) in order to decrease multipath effect by the indoor environment. The proposed positioning algorithm is very simple and requires only the AP(Access Point) coordinates. In addition, it requires only two APs for estimating the location of a smartphone user. The proposed algorithm is a practically applicable technology without any additional hardware and kernel modification in the smartphone. In the experiment results, the reliability of the positioning system was found to be within 0.83 m.

• Smart Structures and Systems
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• v.19 no.2
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• pp.195-202
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• 2017

The aim of this paper is to develop a novel method to determine the severity of a damage in a thin plate. This paper presents a novel fault detection and diagnosis approach employing a new electromagnetic acoustic transducer, called EMAT, together with a complex signal processing method. The method consists in the recognition of a fault that exists within the structure, the fault location, i.e. the identification of the geometric position of damage, and the determining the significance of the damage, which indicates the importance or severity of the defect. The main scientific novelties presented in this paper is: to develop of a new type of electromagnetic acoustic transducer; to incorporate wavelet transforms for signal representation enhancements; to investigate multi-parametric analysis for noise identification and defect classification; to study attenuation curves properties for defect localization improvement; flaw sizing and location algorithm development.

• BMB Reports
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• v.48 no.4
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• pp.200-208
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• 2015

The field of redox proteomics focuses to a large extent on analyzing cysteine oxidation in proteins under different experimental conditions and states of diseases. The identification and localization of oxidized cysteines within the cellular milieu is critical for understanding the redox regulation of proteins under physiological and pathophysiological conditions, and it will in turn provide important information that are potentially useful for the development of novel strategies in the treatment and prevention of diseases associated with oxidative stress. Antioxidant enzymes that catalyze oxidation/reduction processes are able to serve as redox biomarkers in various human diseases, and they are key regulators controlling the redox state of functional proteins. Redox regulators with antioxidant properties related to active mediators, cellular organelles, and the surrounding environments are all connected within a network and are involved in diseases related to redox imbalance including cancer, ischemia/reperfusion injury, neurodegenerative diseases, as well as normal aging. In this review, we will briefly look at the selected aspects of oxidative thiol modification in antioxidant enzymes and thiol oxidation in proteins affected by redox control of antioxidant enzymes and their relation to disease. [BMB Reports 2015; 48(4): 200-208]

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.9
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• pp.78-84
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• 2020

This paper introduces the structural and vibration analysis performed in the localization development process of hydraulic pumps used in wheeled armored vehicles. The maximum strain, maximum stress, maximum displacement, and minimum safety factor were calculated using structural analysis. Furthermore, it was found that the dangerous resonance frequency was avoided through vibration analysis. In addition, the reliability of the analysis results was verified by passing various tests, such as the actual vibration test and the actual durability test. The developed hydraulic pump is expected to contribute significantly to the maintenance of military vehicles in the future.

• Applied Microscopy
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• v.28 no.3
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• BMB Reports
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• v.37 no.3
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• pp.339-342
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• 2004

Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively abeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.

• Korean Journal of Plant Tissue Culture
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• v.27 no.4
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• pp.269-282
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• 2000

Benzylisoquinoline alkaloids are a diverse group of natural products that include many pharmacologically active compounds produced in a limited number of plant families. Despite their complexity, intensive biochemical research has extended our knowledge of the chemistry and enzymology of many important benzylisoquinoline alkaloid pathways, such as those leading to the analgesic drugs morphine and codeine, and the antibiotics sanguinarine and berberine. The use of cultured plant cells as an experimental system has facilitated the identification and characterization of more than 30 benzylisoquinoline alkaloid biosynthetic enzymes, and the molecular cloning of the genes that encode at least 8 of these enzymes. The recent expansion of biochemical and molecular technologies has creat-ed unique opportunities to dissect the mechanisms involved in the regulation of benzylisoquinoline alkaloid biosynthesis in plants. Research has suggested that product accumulation is controlled by the developmental and inducible regulation of several benzylisoquinoline alkaloid biosynthetic genes, and by the subcellular compartmentation of biosynthetic enzymes and the intracellular localization and trafficking of pathway intermediates. In this paper, we review our current understanding of the biochemistry, cell biology, and molecular regulation of benzylisoquinoline alkaloid biosynthesis in plants. We also summarize our own research activities, especially those related to the establishment of protocols for the genetic transformation of benzylisoquinoline alkaloid-producing species, and the development of metabolic engineering strategies in these plants.