• BMB Reports
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• 제43권6호
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• pp.432-437
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• 2010

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes ${\alpha}B$-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with ${\alpha}B$-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, ${\alpha}B$-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or ${\alpha}B$-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both ${\alpha}B$-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and ${\alpha}B$-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21.

• 대한지리학회지
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• 제29권2호
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• pp.117-136
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• 1994

오늘날 첨단산업의 발전과 더불어 산업의 공간구조 변화가 지역적인 차원은 물론 국제적인 차원에서 일어나고 있으며 신산업지구의 등장은 그 좋은 예라고 볼 수 있다. 신산 업지구는 소기업 중심의 유연적 전문화에 의해서만 형성되는 것이 아니라 생산체계, 생산의 네트워크, 뿌리내림(embeddedness), 기업의 규모 등 4가지 요인과 관련하여 다양한 형태로 형성발전하는 역동성을 가지고 있다. 신산업지구는 기본적으로 네트워크의 유형에 따라 4개 의 기본유형과 5개의 혼합형으로 구분될 수 있는데, 이들은 위의 4가지 요인 외에도 기업간 권한의 계층성, 협력과 경쟁, 제도적 요인, 매몰비용(sunk costs) 등에 의해서 그 특성이 구 분되며 사례를 확인할 수 있다. 9개의 유형은 모두 첨단산업과 관련지을 수 있으나, 첨단산 업의 발전과 더불어 혼합형 산업지구가 형성발전될 가능성이 더 크다. 과거의 산업지구는 역사적 우연성에 의해서 형성되는 경우가 흔하였지만 오늘날 첨단산업과 관련한 산업지구는 정책과 전략이 그 형성과 발전에 중요하다. 따라서 제도적 지원체계의 마련, 매몰비용의 절 감, 국제화에 대한 대응 등의 여러 가지 전략이 추진되어야 신산업지구의 형성과 역동적인 발전이 가능하다.

• Molecules and Cells
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• 제40권4호
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• pp.271-279
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• 2017

Ran-binding protein family member, RanBP9 has been reported in various basic cellular mechanisms and neuropathological conditions including schizophrenia. Previous studies have reported that RanBP9 is highly expressed in the mammalian brain and retina; however, the role of RanBP9 in retinal development is largely unknown. Here, we present the novel and regulatory roles of RanBP9 in retinal development of a vertebrate animal model, zebrafish. Zebrafish embryos exhibited abundant expression of ranbp9 in developing brain tissues as well as in the developing retina. Yeast two-hybrid screening demonstrated the interaction of RanBP9 with Mind bomb, a component of Notch signaling involved in both neurogenesis and neural disease autism. The interaction is further substantiated by co-localization studies in cultured cells. Knockdown of ranbp9 resulted in retinal dysplasia with defective proliferation of retinal cells, downregulation of neuronal differentiation marker huC, elevation of neural proliferation marker her4, and alteration of cell cycle marker p57kip2. Expression of the $M{\ddot{u}}ller$ glial cell marker glutamine synthase was also affected in knockdown morphants. Our results suggest that Mind bomb-binding partner RanBP9 plays a role during retinal cell development of zebrafish embryogenesis.

• 한국발생생물학회지:발생과생식
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• 제17권2호
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• pp.99-109
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• 2013

Trophoblasts, in the placenta, play a role for placental development as well as implantation in the early pregnancy. The characteristics and functions of trophoblast are identified by their localization and potency for proliferation, differentiation, and invasion. Thus, inadequate trophoblast cell death induces trophoblast dysfunction resulting in abnormal placental development and several gynecological diseases. Recently, it was reported that increased immortalization-upregulated protein-2 (IMUP-2) by hypoxia influences trophoblast apoptosis. However, IMUP-2 function on autophagy, which is type II programmed cell death remains unclear. In this study, we analyzed IMUP-2 expression in trophoblast cells (HTR8-SVneo) and compared IMUP-2 effects on cell death including apoptosis and autophagy in trophoblast regardless of IMUP-2 expression. Increased IMUP-2 in trophoblast by IMUP-2 gene transfection induces cell death, especially, apoptosis increases more than autophagy (p<0.05). However, the decreased IMUP-2 in trophoblasts after siRNA treatment decreased apoptosis with the decreased activities of caspase 3 and 7. The expressions of LC3 and MDC as an autophagosome makers and phosphorylated mTOR, which is a negative regulator for autophagy, increased. In addition, the S phase of cell cycle increased in trophoblasts when IMUP-2 expression decreased. Taken together, the alteration of IMUP-2 can control the balance between apoptosis and autophagy of trophoblasts resulting in functional involvement in placental development and in gynecological diseases by regulating the function of trophoblasts.

• 디자인학연구
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• 제19권3호
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• pp.183-194
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• 2006

It is expected that the typical lifestyle of the future will be transformed into an opulent and comfortable existence as the quality of life improves due to the increase in household income and reduction in working hours. In the meantime, as the standard of living becomes increasingly more comfortable and plentiful, the toll on physical health becomes magnified as a result of obesity and insufficient exercise caused by super nutrition and change in labor conditions. This has instigated a deep awareness in fitness on the part of many people, forcing them to recognize the significance of daily exercise and physical activity. The Elliptical Cross Trainer(ECT), which has drawn wide attention recently, is a non-impact athletic apparatus that not only promotes exercise of the upper body parts in such sports as skiing but also the exercise of lower parts of the body on a treadmill. It is a type of cross training athletic gear that has been developed for aerobic exercise throughout the entire body. It has already formed a market as big as that of the treadmill in Europe, America, etc. Recently, its demand is growing sharply in the Korean markets as well as those in Northeast Asian countries. Despite such demand increase and expansion, since most of the expensive ECTs are exclusively supplied by suppliers in only a few advanced countries, localization of the ECT is urgently required in order to enhance competitiveness of Korean manufacturers and to expand the market. The ECT development project has been in full swing for approximately two year since 2004 in order to secure independent design, as well as engineering and manufacturing processes in efforts to develop a commercially viable ECT.

• 한국정보시스템학회지:정보시스템연구
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• 제10권1호
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• pp.323-352
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• 2001

Venture business plays a great role in creating national competitive power in every country. In particular, software venture business can mostly belong to manufacturing business, and takes the top seat of core industry as the basis of globalization, informatization and localization as well as the driving force of national competitive power. However, software venture business still has some risks in the whole area of business administration including the process of business starting. There are also problems to be solved in such areas as manager's ability, leadership, talent, level of technical development, marketing channel, recruiting of marketing specialists, financial plan and fund raising. In this respect, this study divides 102 software venture businesses into two; business in the stage of growth and matured business through the analysis of questionnaire and analyzes their present managerial conditions according to their characteristics after classifying them into four categories such as general management, technical development, marketing, finance and accounting. Therefore, this study attempts to provide the basic data useful for business activities of software venture business from now on. The results are as follows; First, it can be judged that software venture businesses make efforts for their technical development in their character, and that they make a great investment in research and development. Consequently, it can be believed that this kind of investment must be continued in the future. Second, it can be seen that they are not much interested in marketing and its manpower. In fact, software venture businesses develop products technically at the risk of their life, while marketing plays an important role in the continuity of business. Thus, it is necessary for them to invest in the supplement of manpower in marketing. Third, it is observed that they generally have some difficulties in raising money from outside. By the way, it. is believed that this problem will be solved by appealing to the persons interested such as investors and creditors, if evaluation system would be developed more exquisitely so that their accounting and technical value may be appraised.

• International Journal of Oral Biology
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• 제41권2호
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• pp.89-96
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• 2016

Tooth development shows dynamic morphological changes from the stages of cap to hard tissue formation and is strictly regulated during development. In the present study, we compared expression and localization of 3 major enamel matrix proteins in rats: amelogenin, enamel and ameloblastin. DD-PCR and RT-PCR revealed differential expression of the major proteins from the cap stage to root stage. Immunofluorescence staining results indicated that amelogenin was not detected in either inner enamel epithelium or reduced enamel epithelium, but highly immunoreactive in preameloblasts and ameloblasts; in addition, it was sporadically expressed in preodontoblasts abutting preameloblasts. Ameloblastin expression was also observed in not only differentiated ameloblasts but also osteoblasts. Immunoreactivity to ameloblastin in ameloblasts was strong in Tomes' processes. Enamelin was exclusively localized along the entire newly formed and maturing enamel. Enamelin was largely localized in near Tomes' processes and enamel rods in maturing enamel. Alendronate treatment resulted in down-regulation of amelogenin and ameloblastin at both transcription and translation levels; whereas, enamelin expression was unchanged in response to the treatment. These results suggested that amelogenin, ameloblastin and enamelin might be implicated in cell differentiation, adhesion of ameloblasts to enamel and enamel crystallization during enamel matrix formation, respectively.

• Mycobiology
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• 제47권4호
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• pp.473-482
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• 2019

Rice blast disease, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most important diseases in rice production. PAS (period circadian protein, aryl hydrocarbon receptor nuclear translocator protein, single-minded protein) domains are known to be involved in signal transduction pathways, but their functional roles have not been well studied in fungi. In this study, targeted gene deletion was carried out to investigate the functional roles of the PAS-containing gene MoPAS1 (MGG_02665) in M. oryzae. The deletion mutant ΔMopas1 exhibited easily wettable mycelia, reduced conidiation, and defects in appressorium formation and disease development compared to the wild type and complemented transformant. Exogenous cAMP restored appressorium formation in ΔMopas1, but the shape of the restored appressorium was irregular, indicating that MoPAS1 is involved in sensing the hydrophobic surface. To examine the expression and localization of MoPAS1 in M. oryzae during appressorium development and plant infection, we constructed a MoPAS1:GFP fusion construct. MoPAS1:GFP was observed in conidia and germ tubes at 0 and 2 h post-infection (hpi) on hydrophobic cover slips. By 8 hpi, most of the GFP signal was observed in the appressoria. During invasive growth in host cells, MoPAS1:GFP was found to be fully expressed in not only the appressoria but also invasive hyphae, suggesting that MoPAS may contribute to disease development in host cells. These results expand our knowledge of the roles of PAS-containing regulatory genes in the plant-pathogenic fungus M. oryzae.

• 한국습지학회지
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• 제21권spc호
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• pp.28-38
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• 2019

한반도의 기후변화는 전 세계 평균보다도 빠르게 진행되고 있다. 최근 빈발하고 있는 태풍 및 극한 강우, 폭설과 한파, 온난화 현상 등이 그 예이다. 특히 북한지역은 오랜 식량난과 에너지난 그리고 무분별한 산림벌목과 개발로 인해 산림생태계가 훼손되어 홍수 및 이수와 같은 기후변화 관련 자연재해에 매우 취약하다. 또한 북한지역은 정치적·사회적인 영향으로 미계측 지역으로 분류되어 있어 수문분석에 필요한 충분한 수문자료를 구하기 어려운 지역이다. 또한 기후변화에 대한 관심이 높아 지면서 각종 수리시설물 및 재해대책에 기후변화를 고려한 연구가 한반도를 대상으로 활발히 진행되고 있지만 북한을 대상으로 수행된 연구사례는 극히 드물다. 따라서 본 연구에서는 관측 수문자료를 획득하기 어려운 북한지역을 대상으로 지역화 모형을 적용기 위해 획득하기 쉬운 유역특성변수를 선정하고 남한의 16개 댐 유역의 기상학적, 지형적 특성을 기반으로 매개 변수 추정식을 산정하였다. 그리고 오십천, 강릉남대천, 남강댐, 영강유역에 적용하여 산정된 매개변수 추정식의 적용성을 검토한 결과 북한지역에 매개변수 추정식의 적용성이 매우 높을 것으로 판단되었다.

• 한국지역지리학회지
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• 제10권2호
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• pp.466-478
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• 2004

사회과 지역학습은 교육과정의 지역화에 부응하고 사회과의 축소판이자 영역 통합의 실험장이라는 점에서 중요한 교육적 의미를 갖는다. 우리나라의 사회과 지역학습은 초등사회과를 중심으로 발전해 왔으나, 현재 보급된 지역교과서는 전국적으로 획일적인 목차구성을 보이는 소극적 의미의 지역화에 머물고 있다. 본 연구에서는 중등학교의 지역학습이 초등사회과의 지역학습과 연계된다는 측면에서, 특히 교사-학생이 주도하는 보다 적극적 의미의 지역화를 지향해야 한다고 주장한다. 이를 위해 지역적 적합화를 위한 이론적 과정을 검토한 후, 서로 다른 지역적 성격을 가진 두 지역을 사례로 하여 지역적 적합화 과정을 비교, 검토하였다. 그리하여, 각 지역의 경관과 지역관련 담론, 생태 환경 특성이 스트랜드의 지역별 비중 조절에 고려되어야 한다는 점과, 이것을 바탕으로 주제 추출의 지역적 적합화 및 내용조직이 진행되어야 한다는 점을 보여주었다. 이러한 이론적 배경을 근거로 이 글의 후반부에서는 지역적으로 차별화된 주제 및 내용조직의 실제를 제시하였다.