• The Korean Journal of Zoology
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• v.33 no.4
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• pp.435-445
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• 1990

Gonadotropin releasing horrnone (GnRH) is known to be extrahypothalamically localized with a broad range including gonad. It remains, however, unknown whether GnRH is locally synthesized in the gonad. The present srudy aims to identity expression and cellular localization of GnRH-Iike mRNA and immunoreactive GnRH in the rat gonad. GnRH radioimmunoassay and chromatographic extracts on G-50 sephadex column showed that rat gonadal extracts contained a substantial amount of immunoreactive GnRH similar to the hypothalamic and synthetic GnRH. Although a wide distribution of immunostainable GnRH-like molecule with different cell types in the rat ovary was observed, the major cell population hybridized with GnRH probe appears to be granulosa. theca cells and corpus luteum. Immunoreactive GnRH-Iike peptides were distributed m various regions of testis, including spermatogenic cells, Sertoli cells and Leydig cells. In situ hybridization revealed that positive signals of GnRH-Iike mRNA were predominandy present in Sertoli cells within some seminiferous tubules, but absent in the outside of seminiferous tubules in the testis. This study clearly demonstrated that GnRH-Iike molecule present in the rat gonad may be resulted from the local synthetic machinery of GnRH supporting the notion that this peptide may act as autocrine and/or paracrine role in intra-gonadal communication.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.4
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• pp.1-10
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• 2011

The purpose of this study is to analyze the guidelines and content of visual landscape planning according to the Landscape Law and to suggest improvements for it. The landscape planning guidelines were analyzed and landscape planning reports were reviewed and compared. To analyze the landscape planning guidelines, we conducted an expert survey and derived the results through statistical methods, such as t-test and importance-performance analysis(IPA). Both landscape planning reports were selected from among metropolitan cities, medium-sized cities, and counties. We analyzed and compared the reports with the landscape planning guidelines. The results of this study are as follows. The stage of visual landscape resource survey progressed systematically, but it was not practical for actual planning because of the excessively detailed guidelines. On the other hand, a greater variety of methods and techniques should be suggested for the landscape structure analysis. At the schematic planning stage, establishing contents suitable for the local government's identity and related plans is necessary. In addition, more detailed guidelines should be included in the landscape planning guidelines. At the master planning stage, the planning qualities and contents differed from cities and counties, so more substantial and detailed guidelines are necessary for the scope and contents. We suggested that some additional items be included such as the synthesis of landscape resource surveys and the viewpoint plan.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.34
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• pp.77-108
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• 2007

The traditional name given to the insurance of third party liabilities and certain contractual liabilities which arise in connection with the operation of ships is protection and indemnity(P & I) insurance. P & I insurance is very different from traditional hull and machinery insurance in that shipowners' hull and machinery insurance is designed primarily to protect the assured against losses to his vessel, whereas P & I insurance seeks to indemnify an shipowner in respect of the discharge of legal liabilities he has incurred in operating his own vessels. This study is to examine the background of establishment of British P & I clubs md, therefore, the identity of P & I insurance. The present British P & I clubs are the remote descendants of the many small and local hull mutual insurance clubs that were formed by British shipowners in the end of 18th century. At that time, British shipowners were dissatified with the state of marine insurance market and, therefore, established clubs together in mutual hull insurance clubs. After the removal of the company monopoly in 1824, greater competition had a good effect on the rates, terms of cover and service offered by the commercial marine insurance market and by Lloyd's underwriters, and the hull clubs became less necessary and went into decline. The burden of British shipowners on liabilities to third parties was steadily increased after the middle of the 19th century, but the amount insured under hull policy was limited in the insured value of the ship. Eventually, the first protection club, that is, the Shipowners' Mutual Protection Society was formed in 1855. It was designed to like past mutual hull clubs, but to cover liabilities for loss of life and personal injury and also the collision risks excluded from the current marine policies, particularly the excess above the limits in hull policies. In 1870, the risks of liability for loss of or damage to cargo carried on board the insured ship was first awarded by the British shipowners. After 1874, many protection clubs formed indemnity club to cover the risk of liability for loss or damage to cargo. As mentioned above, British P & I clubs have been steadily changed according to the response of shipowners under the rapidly changing law of British shipowners' liability, and so on in the future.

• Journal of Microbiology and Biotechnology
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• v.30 no.4
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• pp.526-532
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• 2020

A bacterial strain, designated B301^T and isolated from raw chicken meat obtained from a local market in Korea, was characterized and identified using a polyphasic taxonomic approach. Cells were gram-negative, non-motile, obligate-aerobic coccobacilli that were catalase-positive and oxidase-negative. The optimum growth conditions were 30℃, pH 7.0, and 0% NaCl in tryptic soy broth. Colonies were round, convex, smooth, and cream-colored on tryptic soy agar. Strain B301^T has a genome size of 3,102,684 bp, with 2,840 protein-coding genes and 102 RNA genes. The 16S rRNA gene analysis revealed that strain B301^T belongs to the genus Acinetobacter and shares highest sequence similarity (97.12%) with A. celticus ANC 4603^T and A. sichuanensis WCHAc060041^T. The average nucleotide identity and digital DNA-DNA hybridization values for closely related species were below the cutoff values for species delineation (95-96% and 70%, respectively). The DNA G+C content of strain B301^T was 37.0%. The major respiratory quinone was Q-9, and the cellular fatty acids were primarily summed feature 3 (C_16:1 ω6c/C_16:1 ω7c), C_16:0, and C_18:1 ω9c. The major polar lipids were phosphatidylethanolamine, diphosphatidyl-glycerol, phosphatidylglycerol, and phosphatidyl-serine. The antimicrobial resistance profile of strain B301^T revealed the absence of antibiotic-resistance genes. Susceptibility to a wide range of antimicrobials, including imipenem, minocycline, ampicillin, and tetracycline, was also observed. The results of the phenotypic, chemotaxonomic, and phylogenetic analyses indicate that strain B301^T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter pullorum sp. nov. is proposed. The type strain is B301^T (=KACC 21653^T = JCM 33942^T).

• The Journal of the Korea Contents Association
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• v.18 no.4
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• pp.154-163
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• 2018

The research object is Huayan Street, which is located in Datong City of Shanxi Province in Northern China. And it's detailedly seated to the east of the Huayan Temple. This street is a multicultural area which covers religious and business culture, along with other kinds of civilization since ancient time. The purpose of this research is to restore the historical and cultural tradition, improving the economic development. This research will use the theory of narrative to analyze the public design of Huayan Street. It's present the new design concept to reflect the cultural story of this region. First of all, the cultural stories of Huayan Street were explored and collected by introducing the history. Secondly, the drawbacks of public design of this street were found out by analyzing the current situation in Huayan Street At the end of this paper, a design solution was proposed according to the history and culture with story-telling methods from narratology which depart the street into four scenes opening, developing, changing and concluding. The innovation point of this research is apply humanism into the public design of Huayan Street and rebuild the image of Huayan Street.

• Journal of the Korean association of regional geographers
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• v.8 no.2
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• pp.170-183
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• 2002

Recently it is supposed to be a shift to regional geography. To understand the emergence of the new regional geography, It must be remarked that the progress of capitalist production and consumption change our conceptional apparatus such as representation of space. Region is one of the important mode of representations of space and time. In the Fordist capitalism of 20th century, development is regarded as diffusion of capitalist expansion of western worlds. State must support capitalist growth through regional policies which include constructing of infrastructure and regionalization of spatial division of labour. The regional development theories contributed as ideology and policy tools for state intervention. The region was simply one of the most logical classification tools of organizing geographical informations. In the theories of regional development, the concept of region was reduced to the formal unit of classification. As the transition from Fordism to flexible accumulation, the region is again acquiring its 'identity' and 'authenticity'. In this tendency of the revival of region, it is expected that good achievement could be made in the field of regional geography through relevant research methods. It is also believed one of the available means are historical approaches to the cultural and ecological regions. The historical approaches to cultural and ecological regions are not only correspondent with cultural development strategies of local governments, but also could convey regional identities through both narrativization of place and aestheticization of landscape.

• The Journal of the Korea Contents Association
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• v.5 no.3
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• pp.103-111
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• 2005

By international lighting market's growth and domestic landscape lighting market's growth, the latest lighting market is activated. But, They are doing price competition that domestic lighting technology and development technology of lighting fixtures design do not possess international competitiveness than these change. Specially, as image of area and the country can be influenced greatly by cityscape at present visual point that many interests are converged country and identity establishment of local government, many cities are using force hereupon. Constructs which cityscape is various including natural ecology area of life environment gather and are done Image creation and this people's consciousness and culture living soon inside thing which do speaking by proxy be. Than city's night view nature lighting more than by spectacle lighting along with life lighting to basis materialize can. It is meaning more than thing that fine view of city becomes beautiful simply that night view of city becomes superior, that means that city efficiency and quality of life are exalted by a word. Wish to establish effective lighting technology and new product design process through PLS lighting technical development and 300 W lighting fixtures design development hereupon, and raise domestic spectacle lighting market's localization and domestic lighting market's competitive power furthermore.

• The Journal of the Korea Contents Association
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• v.16 no.5
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• pp.229-241
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• 2016

This research has its purpose to seek, among the CEOs of social enterprises, the influence of job stress on business performance, social support inbetween them, and adjusting role of government-supported policies. CEO of social enterprise, which is established to realize its social purposes, receives the job stress due to business ethics, profitability, identity crisis, management challenges and etc. We would like to set the foundation for the enterprises to constantly conduct social purposes within the local society by raising the business performances of social enterprises through seeking the measures to resolve such job stresses of the CEOs by expressing them. This research applied the ISR Job Stress Model theroy as its main ground of theoretical background. We would like to analyze the inter-significance by setting the job stress causes and job stress influences as independent variables, business achievements as a dependent variable, and social support, government-supported policies as moderating variables. For the methods of the research, we collected, using structuralized questionnaire, from 223 social enterprise CEOs located within Korea. Data analysis used IBM SPSS Statristics 20.0 programme, and through general characteristic of sample, confidence testing, factorial analysis, correlation analysis and research hypothesis testing, drew the following results. First, job stress factor and influence were analyzed to have negative impacts on business performance. Second, social support was found to lower job stress factor and influence, and raise business performance. Third, government-supported policy was analyzed to lower job stress factor and influence, and raise business performance.

• Korean Journal of Childcare and Education
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• v.5 no.2
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• pp.73-90
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• 2009

The number of multi-cultural members, such as immigrant labors, international marriage women are gradually increasing in Japan society. Now it might be said that Japan has become a multi-cultural society. Recently in Japanese kindergartens and nursery schools the numbers of foreign and bi-cultural children are increasing. The aim of this paper is to review the researches on the cultural diversity in the Japan, especially in childcare. Methodology proceeds from a review of related literature to internet-based data and to the statistics issued by the government. Through reviewing them, the problems of teachers' assumptions and the importance of discussions about teachers' competencies to educate minority children and to communicate with their parents were suggested. As a switch over to multi-cultural society is occurring in Japan, the attitude of assimilationism is weakening, and the local community is now beginning to tolerate the multi-cultural childcare. Consequently, It was over to age that is distinguish identity from only nationality.

• Research in Plant Disease
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• v.22 no.1
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• pp.59-63
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• 2016

A new poty-like virus was isolated from plants of winter daphne (Daphne odora) that showed virus-like symptoms on leaves, from four regions of Korea during 2014. Filamentous-shaped particles were observed by transmission electron microscopy of preparations extracted from symptomatic leaves and examined by the direct negative stain method. RT-PCR assay showed that three samples were positive for both Cucumber mosaic virus and potyvirus, and only one sample was positive for potyvirus only. A BLAST comparison to partial sequences from helper-component proteinase, cylindrical inclusion and coat protein genes detected the highest nucleotide identity of 76%, 72%, and 72% with Daphne mosaic virus, respectively, levels below the potyvirus species discrimination threshold. The new potyvirus was isolated using indicator plants (Chenopodium amaranticolor), in which local lesions were produced. In this study, we identified a novel potyvirus from winter daphne, which we have named Daphne mottle virus (DapMoV).