• 제목/요약/키워드: Local Alignment

검색결과 158건 처리시간 0.022초

영상 전처리 수행을 통한 Rededge-M 카메라의 수색 관측에의 활용성 검토 (Evaluation of Rededge-M Camera for Water Color Observation after Image Preprocessing)

  • 김원국;노상현;문용선;정성훈
    • 한국측량학회지
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    • 제37권3호
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    • pp.167-175
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    • 2019
  • 물의 색깔 즉, 수색(水色)을 분석하면 그 물이 함유하고 있는 유색 구성물질의 분포 및 농도를 비파괴적으로 추정할 수 있다. 이러한 분석을 위해 분광복사계를 사용하는데, 최근 드론, 자율운행차, 헬리카이트 등의 저고도 무인 플랫폼에 장착하기 위한 경량 다분광 카메라에 대한 수요가 높아지고 있다. 본 연구에서는 최근 활용 용이성으로 인해 지역적 환경 변화를 모니터링함에 있어 그 활용도가 높아진 Micasense사의 Rededge-M 다분광 카메라의 전처리를 수행하고, 수색관측에의 활용성을 검증하기 위한 분석을 수행하였다. 우선, 영상 형태로 생성되는 자료에서의 Vignette 보정 및 밴드 정렬을 수행하였고, 수색관측을 위해 필요한 하늘, 물, 태양광을 측정한 복사량 그리고 그로부터 최종적으로 도출되는 원격탐사 반사도 관측치를 독립적인 초분광 센서의 관측값과 비교하여 분석하였다. 실험 결과, 전처리 과정을 수행하였을 시, 청색밴드(475 nm)와 근적외선 밴드(840 nm)에서 주목할 만한 차이가 있었지만, Rededge-M은 전반적으로 수색 분석을 위한 기본적인 광학적 성능을 가지고 있음을 확인하였다.

지도학습 오토인코더를 이용한 전문어의 범용어 공간 매핑 방법론 (Domain-Specific Terminology Mapping Methodology Using Supervised Autoencoders)

  • 윤병호;김준우;김남규
    • 경영정보학연구
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    • 제25권1호
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    • pp.93-110
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    • 2023
  • 최근 비정형 자료인 텍스트를 벡터로 변환하고 이를 통해 다양한 목적으로 방대한 양의 자연어를 분석하는 시도가 이루어지고 있다. 특히 코퍼스 규모가 제한적일 수밖에 없는 전문적인 도메인의 텍스트에 대해서도 분석 수요가 급증하면서, 해당 전문 분야의 문서를 범용 문서와 함께 분석하기 위한 연구가 활발하게 이루어지고 있다. 특정 전문어를 해당 전문어 코퍼스 외부의 일반적인 범용어와 함께 분석하기 위해서는, 전문어 임베딩 공간을 범용어 임베딩 공간과 일치시키는 것이 필요하다. 기존에는 변환 행렬 또는 매핑 함수 등을 통해 전문어 코퍼스로부터 얻은 전문어 임베딩 값을 범용어 임베딩 공간으로 변환, 일치시키려는 시도가 있었지만, 변환 행렬을 기반으로 하는 선형 변환은 국지적인 범위에서만 근사적인 변환 효과가 있다는 일반적인 선형 변환의 한계를 극복하지 못했다. 이러한 선형 변환의 한계를 극복하기 위해 최근에는 다양한 형태의 비선형적인 변환 방법이 제안되고 있으며, 본 연구에서는 오토인코더(Autoencoder)와 회귀 모델을 동시에 학습하는 종단형 학습을 통해 전문어 임베딩 공간을 범용어 임베딩 공간으로 변환하여 임베딩 공간을 일치시키는 모델을 제안한다. 실제 "보건의료" 분야의 R&D 문서에 대해 임베딩 변환 실험을 진행한 결과, 제안 방법론이 기존의 오토인코더를 활용한 방법 대비 변환 정확도 측면에서 우수한 성능을 보임을 확인하였다.

Cloning and Characterization of a Heterologous Gene Stimulating Antibiotic Production in Streptomyces lividans TK-24

  • Kwon, Hyung-Jin;Lee, Seung-Soo;Hong, Soon-Kwang;Park, Uhn-Mee;Suh, Joo-Won
    • Journal of Microbiology
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    • 제37권2호
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    • pp.102-110
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    • 1999
  • Genetic determinant for the secondary metabolism was studied in heterologous expression in Streptomyces lividans TK-24 using Streptomyces griseus ATCC 10137 as a donor strain. Chromosomal DNA of S. griseus was ligated into the high-copy number Streptomyces shuttle plasmid, pWHM3, and introduced into S. lividans TK-24. A plasmid clone with 4.3-kb BamHI DNA of S. griseus (pMJJ201) was isolated by detecting for stimulatory effect on actinorhodin production by visual inspection. The 4.3-kb BamHI DNA was cloned into pWHM3 under the control of the strong constitutive ermEp promoter in both directions (pMJJ202); ermEp promoter-mediated transcription for coding sequence reading right to left: pMJJ203; ermEp promoter-mediated transcription for coding sequence reading left to right) and reintroduced into S. lividans TK-24. The production of actinorhodin was markedly stimulated due to introduction of pMJJ202 on regeneration agar. The introduction of pMJJ202 also stimulated production of actinorhodin and undecylproidigiosin in submerged culture employing the actinorhodin production medium. Introduction of pMJJ203 resulted in a marked decrease of production of the two pigments. Nucleotide sequence analysis of the 4.3-kb region revealed three coding sequences: two coding sequences reading left to right, ORF1 and ORF2, one coding sequence reading right to left, ORF3. Therefore, it was suggested that the ORF3 product was responsible for the stimulation of antibiotic production. The C-terminal region of ORF3 product showed a local alignment with Myb-related transcriptional factors, which implicated that the ORF3 product might be a novel DNA-binding protein related to the regulation of secondary metabolism in Streptomyces.

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Comparison of Trunk Strategy to Maintain Balance during the One-Leg Stance on a Medio-Lateral Ramp and an Anterior-Posterior Ramp

  • Lee, Sang-Yeol;Lee, Myoung-Hee
    • The Journal of Korean Physical Therapy
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    • 제29권4호
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    • pp.223-226
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    • 2017
  • Purpose: This study examined two trunk strategies - medio-lateral ramp and anterior-posterior ram - and their effects on pelvis and trunk movements, providing basic material for safe ramp utilization. Methods: The present study included 20 asymptomatic males recruited from a local university. Participants were asked to stand with their feet shoulder-width apart. Their dorsal side faced the camera, and measurements were performed while standing in a neutral double stance, one leg stance, $15^{\circ}$ of medial and lateral ramp, and $15^{\circ}$ of anterior and posterior ramp. Participants were allowed to practice for 3 minutes, and each participant had a 30 seconds rest between the two ramp conditions. One-way repeated measures analysis of variance was used to determine the effects the ramp conditions on spinal alignment. In all analyses, p<0.05 was used to indicate statistical significance. Results: The trunk-inclination angle on the posterior ramp was significantly lower than that of the double stance position (p<0.05). The trunk imbalance angle was significantly higher on the medial ramp, than that on the double stance position (p<0.05). The pelvic position and pelvic torsion angles were significantly higher in the medial, lateral, and anterior ramp positions than that of the double stance position (p<0.05). The pelvic rotation angles on the medial, lateral, and anterior ramps were significantly lower than that of the double stance position (p<0.05). Conclusion: These findings suggest that when people are exposed to the same form of ramp for an extended period, posture modifications may be triggered.

정맥기형 환자에 있어서의 유전자 발현 차이 (Difference of Gene Expression in Venous Malformation)

  • 김택균;오은정;조병채;정호윤
    • Archives of Plastic Surgery
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    • 제34권6호
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    • pp.671-678
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    • 2007
  • Purpose: Venous malformation(VM) which often causes pain and discomfort is the most common type of vascular malformations. Although it is presented with disfigured appearance and associated soft tissue or skeletal hypertrophy, the molecular bases of VMs are poorly understood. Differentially expressed genes(DEGs) of VMs were investigated to illuminate the molecular mechanism of the disease entity. Methods: Gene expressions of VM patients' subcutaneous tissue were studied in comparison with normal persons' by $GeneFishing^{TM}$ technique using the annealing control primers (ACPs) to identify DEGs. Candidate genes were sequenced and screened by basic local alignment search tool (BLAST) afterwards. Results: Among seventy DEGs identified, forty DEGs which had shown significantly different expression pattern were sequenced. Twenty eight out of 40 were up-regulated while 12 were down-regulated. BLAST searches revealed that 37 were known genes and 3 were unknown genes. Many genes were involved in the differentiation and remodeling of smooth muscle cells, opposed to the previous hypothesis that a lot of angiogenetic genes would be involved. Furthermore, several transcription factors and related genes, as well as cell signaling and metabolism regulators, were up regulated. Conclusion: It suggests that analysis of DEGs in VMs provide basic knowledge about its pathophysiology. and new therapeutic approaches.

유기성 폐기물의 발생 악취 제거를 위한 Delftia sp.의 성장조건 최적화 (Growth Optimization of Delftia sp. for the Odor Control of Organic Waste)

  • 권혁구;정준오;추덕성;이장훈
    • 한국환경보건학회지
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    • 제35권5호
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    • pp.393-401
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    • 2009
  • We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

무릎 자기공명영상에서 지역적 확률 아틀라스 정렬 및 반복적 그래프 컷을 이용한 전방십자인대 분할 (Anterior Cruciate Ligament Segmentation in Knee MRI with Locally-aligned Probabilistic Atlas and Iterative Graph Cuts)

  • 이한상;홍헬렌
    • 정보과학회 논문지
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    • 제42권10호
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    • pp.1222-1230
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    • 2015
  • 무릎 자기공명영상에서 전방십자인대의 분할은 밝기값의 불균일성 및 주변 조직들과의 유사 밝기값 특성으로 인해 기존 분할기법의 적용에 한계가 있다. 본 논문에서는 지역적 정렬을 통한 확률아틀라스 생성 및 반복적 그래프 컷을 통한 다중아틀라스 기반 전방십자인대 분할기법을 제안한다. 첫째, 전역 및 지역적 다중아틀라스 강체정합을 통해 전방십자인대의 확률아틀라스를 생성한다. 둘째, 생성된 확률아틀라스를 이용하여 최대사후추정 및 그래프 컷을 통하여 전방십자인대 초기 분할을 수행한다. 셋째, 마스크 기반 강체정합을 통한 형상정보 개선 및 반복적 그래프 컷을 통해 전방십자인대 분할 개선을 수행한다. 제안방법의 성능평가를 위하여 육안평가 및 정확성평가를 수행하였으며, 평가 결과 제안방법의 Dice 유사도는 75.0%, 평균표면거리는 1.7화소, 제곱근표면거리는 2.7화소로서 기존 그래프 컷 방법에 비하여 전방 십자인대의 분할정확도가 각각 12.8%, 22.7%, 및 22.9% 향상된 것으로 나타났다.

Identification and Expression Patterns of fvexpl1, an Expansin-Like Protein-Encoding Gene, Suggest an Auxiliary Role in the Stipe Morphogenesis of Flammulina velutipes

  • Huang, Qianhui;Han, Xing;Mukhtar, Irum;Gao, Lingling;Huang, Rongmei;Fu, Liping;Yan, Junjie;Tao, Yongxin;Chen, Bingzhi;Xie, Baogui
    • Journal of Microbiology and Biotechnology
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    • 제28권4호
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    • pp.622-629
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    • 2018
  • Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins have rarely been discussed. In the present study, an expansin-like protein-encoding fvexpl1 gene, was identified from Flammulina velutipes by using local BLAST. It consisted of five exons with a total length of 822 bp. The deduced protein FVEXPL1 contained 274 amino acids with a predicted molecular mass and isoelectric point of 28,589 Da and pH 4.93, respectively. The first 19 amino acids from the N terminal are the signal peptide. Phylogenetic analysis and multiple protein alignment indicated FVEXPL1 was an expansin-like protein. The expression level of fvexpl1 gene in the stipe was significantly higher than that in the mycelia, primordia, and cap. However, the expression level of fvexpl1 gene was significantly higher in the fast elongation region of the stipe as compared with the slow elongation region. Expression analysis indicated that fvexpl1 gene might have an auxiliary role in the stipe morphogenesis of F. velutipes.

Comparison of microstructures in T1-1223/Ag tapes with different chemical compositions and J$_c$'s

  • Jeong, D.Y.;Kim, H.K.;Lee, H.Y.;Cha, M.K.;Ha, H.S.;Oh, S.S.;Tsuruta, T.;Horiuchi, S.
    • 한국초전도학회:학술대회논문집
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    • 한국초전도학회 1999년도 High Temperature Superconductivity Vol.IX
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    • pp.280-290
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    • 1999
  • The microstructures of a Tl$_{0.8}Pb_{0.2}Bi_{0.2}Sr_{1.6}Ca_2Cu_3O_{9+{\delta}}$/Ag tape (tape I ) with J$_c$ of 17,600 A/cm$^2$ at 77 K and 0 T and three Tl$_{0.8}Pb_{0.2}Bi_{0.2}Sr_{1.8}Ba_{0.2}Ca_{2.2}Cu_3O_{9+{\delta}}$/Ag tapes with J$_c$'s of 9,300 (tape II), 16,700 (tape III) and 25,200 A/cm$^2$ (tape IV)prepared using the powder-in-tube method and an in-situ reaction method, were investigated using scanning electron microscopy and high-resolution transmission electron microscopy, and compared each other. ln the tape preparation, an intermediate rolling process was incorporated during final heat-treatment for the last tape, but not for the rest of the tapes. The microstructural analysis revealed clear differences in grain-texturing, crystallographic defects and impurity phases, depending on the chemical composition of the tape. Tendency of directional grain-alignment increased in an order of tapes I, II III and IV. In tape IV, T1-1223 grains are textured, at least in local regions. In crystallographic defects, while stacking faults were prevalent in the former composition, dislocations and voids were frequently observed in the latter. Also impurity phases were appeared to be more abundant in the former than in the latter. The relationship between 1,and the microstructure in the tapes was attempted to explain in a term of grain-linking.

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대전광역시 대전천과 갑천 및 유등천의 물과 주변 토양들로부터 야생효모들의 분리 및 분포 특성 (Isolation and Diversity of Wild Yeasts from the Waters and Bank Soils of Daejeoncheon, Gapcheon, and Yudeungcheon in Daejeon Metropolitan City, Korea)

  • 한상민;이상엽;김하근;이종수
    • 한국균학회지
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    • 제45권4호
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    • pp.259-269
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    • 2017
  • 대전광역시 하천들의 효모 종 다양성을 조사하기 위하여 먼저 대전광역시 동남부와 시내를 흐르는 대전천과 서남부의 갑천에 있는 물과 주변 토양들을 2016년 8월과 2017년 2월에 각각 148점을 동일 장소에서 채취하여 야생효모들을 분리, 동정하였다. 여름 채취 물과 주변 토양 74점에서 23종 37균주의 야생효모들이 분리된 반면에 겨울 채취 시료들에서는 약 2배 이상 많은 34종 83균주가 분리되었다. 이들 3대 하천에서 가장 많이 분리된 야생효모는 Candida속 균으로 전체 42%이었고 Cryptococcus속 균과 식품에서 주로 분리되는 Saccharomyces속 균들이 각각 7%와 6%로 많이 분리되었다. 종 수준에서는 Candida tropicalis가 25균주로 가장 많았으며 여름 시료와 겨울 시료에서 공통으로 분리된 효모들은 Aureobasidium pullulans 등 13종이었다.