• Journal of Korean Society of Rural Planning
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• v.7 no.2 s.14
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• pp.3-11
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• 2001

A portable GPS receiver and a navigation software were used to investigate land application practices of manure composts. The field investigation indicated that most of the land application of manure composts were observed before April. Plowing for incorporation of manure with soil were continued from the middle of April. The results also revealed that over 60% of the farms were within 1 km from livestock farms from which manure composts were transported. Most of the farms having land application were dry field rather than paddies, Some of fields located near livestock farms frequently experience over-application of manure composts. It seems to have been difficult to complete the investigation in this study without the Gps/navigation system. The GPS/navigation system will become an essential tool in field investigations such as ecological/environmental monitoring works that require repeated observation at a number of sites.

• Journal of Food Hygiene and Safety
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• v.34 no.6
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• pp.557-561
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• 2019

This study investigated the efficacy of DNA extraction methods for real-time PCR detection of foodborne pathogenic bacteria in livestock manure composts. Livestock manure composts were inoculated with Escherichia coli O157:H7, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus and incubated in enrichment broth. For DNA extraction, enriched samples were treated following boiling method, by chloroform, C₁₈ powder, and proteinase K. As a result, 4 species of bacteria were detected by real-time PCR when subjected to boiling for 30 min and treated with proteinase K. These results suggest that detection of foodborne pathogens by real-time PCR from livestock manure composts could be applicable using effective DNA extraction methodology such as the boiling method or proteinase K.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.409-413
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• 2014

BACKGROUND: Large amount of veterinary antibiotics have been used in the livestock industry to prevent diseases and promote growth. These antibiotics are excreted through feces and urine in unchanged form and reach to agricultural fields via application of the livestock manure based composts. The purpose of this study was to evaluate the occurrence of tetracyclines-resistant bacteria in the soil received livestock manure compost for a long term. METHODS AND RESULTS: Tetracyclines (tetracycline TC, chlortetracycline CTC, and oxytetracycline OTC) resistance bacteria in the soil of rice-onion field applied pig manure compost (PM), in the soil of grass-rye field received cow manure compost (CM), and in the soil of rice field applied inorganic fertilizer (NPK) were determined. The soil received livestock manure composts clearly showed higher number of TC, CTC, and OTC resistance bacteria compared with the soil treated with inorganic fertilizer. The antibiotic resistant bacteria recovered appeared at 80 mg/L of tetracyclines was identified 1 specie, 6 genera 7 species, and 6 genera 7species in the soils received CM, PM, and NPK, respectively. The dominant resistant bacteria with the CM and PM application were Ochrobactrum and Rhodococcus. CONCLUSION: The application of livestock manure compost in the agricultural field is likely to contribute the occurrence of antibiotic resistance bacteria in the agricultural environment.

• Journal of Korean Society of Rural Planning
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• v.7 no.2 s.14
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• pp.65-73
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• 2001

Animal manure and manure compost produced from livestock farms are widely applied in crop lands. Leachate and runoff water from them are presumed to be one of the major sources of water quality deterioration in rural streams. Laboratory experiments were conducted to investigate water quality characteristics and loading of leachate obtained under rainfall simulation. Manure composts for the experiments were collected from beef cattle farms, dairy farms, and pig farms. Water quality parameters include SS, COD, TN, and TP. Most parameters of leachate from pig manure compost was higher than others. Both TN and TP concentrations were reflecting the composition of manure itself. It is recommended, therefore that the leachate from the manure composts should be controlled not to be discharged into streams.

• Journal of Industrial Technology
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• v.13
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• pp.3-17
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• 1993

The composting process is a suitable to dispose the livestock manure in terms of resources recovery. However the performence of composting process is greatly affected by the environmental conditions such as characteristics of manure, type of the bulking agent, initial moisture contents, temperature, recycle and so on. The purpose of this study is to evaluate the optimum environmental conditions of composting process for livestock manures. The analytical results indicated that no bulking agent was necessary for the cow manure because of the proper C/N ratio. However the pig manure required a bulking agent since the pig manure had not only low in C/N ration but poor ventilation characteristics. In addtion, the initial miosture content for optimum composting appeared to be about 60%. The temperature control was also an essential factor to enhance the activity of thermophilic microorganisms in the laboratory composting unit. It was further found that the recycle of composts may contributed the completion of composting precess as well as C/N ratio reduction and moisture control.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.824-829
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• 2011

In recent years, there has been an increasing public concern about fecal contamination of water, air and agricultural produce by pathogens residing in organic fertilizers such as manure, compost and agricultural by-products. Efforts are now being made to control or eliminate the pathogen populations at on-farm level. Development of efficient on-farm strategies to mitigate the potential risk posed by the pathogens requires data about how the pathogens prevail in livestock manure composts and organic fertilizers. Microbiological analysis of livestock manure composts and organic fertilizers obtained from 32 and 28 companies, respectively, were conducted to determine the total aerobic bacteria count, coliforms, Escherichia coli count and the prevalence of Staphylococcus aureus, Bacillus cereus, Salmonella spp., Escherichia coli O157:H7, Listeria monocytogenes, and Cronobacter sakazakii. The total aerobic bacteria counts in the livestock manure composts and organic fertilizers were in the range of 7 to $9log\;CFU\;g^{-1}$ and 4 to $6log\;CFU\;g^{-1}$, respectively. In the livestock manure composts, coliforms and E. coli were detected in samples obtained from 4 and 2 companies, respectively, in the range of 2 to $5log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In the organic fertilizers, coliforms and E. coli were detected in samples obtained from 4 and 1 companies, respectively, in the range of 1 to $3log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In 3 out 32 compost samples, B. cereus was detected, while other pathogens were not detected. In 28 organic fertilizers, no pathogens were detected. The complete composting process can result in the elimination of pathogens in livestock manure compost and organic fertilizer. The results of this study could help to formulate microbiological guidelines for the use of compost in environmental-friendly agriculture. This research provides information regarding microbiological quality of livestock manure compost and organic fertilizer.

• Animal Bioscience
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• v.36 no.4
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• pp.671-678
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• 2023

Objective: Previous studies isolated the thermophilic ammonium-tolerant (TAT) bacterium Bacillus sp. TAT105 that grew in composting swine manure with the assimilation of ammonium nitrogen and reduced ammonia emissions during composting. Those studies also investigated the potential for applications of TAT105 to composting. It was observed that the concentration of TAT bacteria, phylogenetically close to TAT105, increased during composting. The objectives of this study were to identify the phylogenetic placement of these TAT bacteria and investigate their distribution in various composts. Methods: The phylogenetic placement of TAT105 was examined based on the sequence of 16S ribosomal RNA gene. The genomic DNA homology between TAT105 and the type strains of bacterial species that were phylogenetically close to TAT105 were examined by DNA-DNA hybridization. Moreover, the tolerances of these strains to NH₄Cl and NaCl were analyzed using a cultivation method. Concentrations of TAT bacteria in various composts were evaluated using an agar medium specific to TAT bacteria and polymerase chain reaction followed by restriction fragment length polymorphism analysis. Results: TAT105 was most closely related to Bacillus thermolactis and Bacillus kokeshiiformis. Many variants of these species have been detected in various environments, including composts. The type strains of these species displayed TAT characteristics that were similar to those of TAT105. Among the composts examined in this study, TAT bacteria were detected at high concentrations (10⁵ to 10⁹ colony forming units per gram of dry matter) in most of the composts made from cattle manure, swine manure, bark, and excess sludge. Conclusion: TAT bacteria comprised B. thermolactis, B. kokeshiiformis, and their phylogenetically close relatives. They were considered to be adaptable to composting of some certain materials, and a favorable target for searching for strains with some useful function that could be applied to composting of these materials.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.329-334
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• 2010

The characteristics of nitrogen mineralization in upland soil was studied with 27-week incubation at $25^{\circ}C$. The used soils in this experiment were received six kinds of livestock manure compost each year for four years. Six different composts, which were chicken (CHM), pig (PIM), and cow (COM) manure composted without bulking agent, and chicken (CHMS), pig (PIMS), and cow (COMS) manure composted with sawdust as a bulking agent, were selected for this study. The first-order model was fit to the observed mineral nitrogen (N) vs incubation days using a non-linear regression procedure. The soil potential for N mineralization (No) of manure compost (CHM, PIM, and COM) treated soils were higher than those of the manure-sawdust compost (CHMS, PIMS, and COMS) treated soils. The No value of PIM applied soil was 15.0 mg 100 $g^{-1}$, which was the highest value among the treatments. The amount of N mineralized in compost applied soils ranged from 8.1% to 11.9% of the total N content in soils and increased with increasing total N content in soils. The organic matter content in compost applied soils were negatively correlated with No value (r = $-0.69^*$). Therefore, our result indicated that determination of N application rate in livestock manure compost applied soil should be based on total nitrogen content better than soil organic matter content.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.1
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• pp.113-118
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• 2009

We investigated the structure of bacterial communities present in livestock manure-based composting processes and evaluated the bacterial succession during the composting processes. Compost samples were derived separately from swine manure, dairy manure and sewage sludge. The structure of the bacterial community was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) using universal eubacterial primers. The genus Bacillus and related genera were mainly detected following the thermophilic composting phase of swine and dairy manure composts, and the members of the phylum Bacteroidetes were mainly detected in the cattle manure waste-based and sewage sludge compost. We recovered and sequenced limited number of the bands; however, the PCR-DGGE analysis showed that predominant diversities during the composting processes were markedly changed. Although PCR-DGGE analysis revealed the presence of different phyla in the early stages of composting, the members of the phylum Firmicutes and Bacteroidetes were observed to be one of the predominant phyla after the thermophilic phase.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.1
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• pp.8-14
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• 2015

Livestock waste compost tea is a liquid extract of compost obtained by mixing livestock compost. In this study, some chemical and microbiological characteristics of compost tea depending on the kind of raw materials used were examined, and several experiments to investigate the practical effects on Chinese cabbage cultivation were conducted. This experiment showed that livestock composts needed to be added into aerated water at the ratio between 1:100 and 1:10 (1 part compost to 10~100 parts water) to produce the high quality compost tea. Compost teas must be aerated more than 24 to 48 hours to be able to support aerobic organisms. In cultivation test with compost teas, swine manure compost teas were made by the extracting ratio of 50x, in the aerated condition for 24 hours in water and oil cake in the extracting ratio of 100x were added as supplements. Following the input of oil cake, the concentration of nitrogen and aerobic bacteria increased. Another experiment was conducted to determine the effect of different swine manure compost teas on plant growth and yield of Chinese cabbage. The fresh yield of Chinese cabbage was higher in the fertigated plots by compost tea with oil cake compared to those of N, $P_2O_5$, $K_2O$ fertilization plot with chemical fertilizer by soil test recommendation (Fert. NPK). The effect of compost tea on growth of Chinese cabbage was largely attributable to the increased number of microorganisms as well as nutrients.