• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.735-739
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• 2002

Large quantities of porcine hepatocyte aggregates with various degrees of aggregation (DA) could be obtained by controlling the suspension periods (0,9,24, and 48 h), and by entrapping the hepatocyte aggregates in model materials of encapsulation such as Ca-alginate and type-I collagen gels. The effects of DA on liver-specific functions of hepatocytes were evaluated in order to obtain optimum DA for the cell source of bioartificial liver (BAL) systems. Irregular rugged aggregates (size $75 \pm 28$ $\mu\textrm{m}$) farmed by 24 h of suspension culturing showed peak viability and hepatic functions such as ammonia removal and albumin secretion in the two types of entrapment systems, thus offering themselves as a stable cell source of a BAL system for hepatic functions and scale-up.

• 약학회지
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• 제37권2호
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• pp.149-157
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• 1993

Protein methylase inhibitor which is a modulator of biological methylation has been purified and characterized from porcine liver soluble fraction by cell fractionation, Sephadex G25 chromatography, reverse phase HPLC, size exclusion HPLC. The results are summarized as follows. 1) The purified inhibitor shows apparent homogeneity, as judged by HPLC. 2) A molecular weight of the purified inhibitor which is composed of 18 amino acid residues is about 1,400 daltons. 3) A single absorption peak of ultraviolet spectrum was observed at 260nm. 4) The inhibitor was not inactivated by heating at $100^{\circ}C$ until 60min. and its activity was not influenced by treatment with digestive enzymes, such as trypsin, pepsin, pronase, chymotrypin, lysozyme, DNase, and RNase. 5) The purified inhibitor inhibited protein rnethylase I, II, III and phospholipid methyltransferase activities. 6) The purified inhibitor inhibited noncompetitively protein methylase II from porcine liver, spleen, and testis. 7) The $K_{i}$ values for protein methylase II from porcine liver, spleen, and testis were 300nM, 250nM, 297nM, respectively.

• Molecules and Cells
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• 제45권5호
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• pp.343-352
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• 2022

The advent of the assay for transposase-accessible chromatin using sequencing (ATAC-seq) has shown great potential as a leading method for analyzing the genome-wide profiling of chromatin accessibility. A comprehensive reference to the ATAC-seq dataset for disease progression is important for understanding the regulatory specificity caused by genetic or epigenetic changes. In this study, we present a genome-wide chromatin accessibility profile of 44 liver samples spanning the full histological spectrum of nonalcoholic fatty liver disease (NAFLD). We analyzed the ATAC-seq signal enrichment, fragment size distribution, and correlation coefficients according to the histological severity of NAFLD (healthy control vs steatosis vs fibrotic nonalcoholic steatohepatitis), demonstrating the high quality of the dataset. Consequently, 112,303 merged regions (genomic regions containing one or multiple overlapping peak regions) were identified. Additionally, we found differentially accessible regions (DARs) and performed transcription factor binding motif enrichment analysis and de novo motif analysis to determine new biomarker candidates. These data revealed the gene-regulatory interactions and noncoding factors that can affect NAFLD progression. In summary, our study provides a valuable resource for the human epigenome by applying an advanced approach to facilitate diagnosis and treatment by understanding the non-coding genome of NAFLD.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1996년도 정기총회 및 학술발표회
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• pp.6-6
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• 1996

Gap junction channels were reconstituted into unilamellar liposomes using immunoaffinity purified connexin 32 gap junction protein from rat liver. Vesicles containing open channels and close channels were separated by means of iso-osmolar sucros density gradient sedimentation. The open channels formed in lipid vesicles were permeable to a fluorescent dye molecule, lucifer yellow of which the hydrodynamic size is similar to pore size of gap junctions in vivo. (omitted)

• 핵의학기술
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• 제22권1호
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• pp.15-22
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• 2018

PET/MRI 검사 시 호흡에 의한 움직임은 영상의 질 저하는 물론 종양의 크기, 표준섭취계수의 오차를 발생시키는 원인이 된다. 본 연구에서는 일체형 PET/MRI 장비에서 MR기반 호흡 움직임 보정 방법의 적용에 따른 종양의 표준섭취계수, 크기와 영상 품질 변화를 평가하고자 하였다. Biograph mMR 3.0T (Siemens, Germany)장비에서 2016년 3월부터 7월까지 $^{18}F-FDG$ liver PET/MRI 검사를 시행한 30명 ($62.5{\pm}10.2$세)의 데이터를 분석하였다. 7분의 PET listmode 데이터를 획득하는 동안 MR 기반 호흡 움직임 보정 방법인 MAG, MTG와 NG T1 weighted MR 영상을 획득하였다. Gated PET 영상의 재구성은 35% efficiency window가 적용된 MAG와 MTG로부터 획득된 감쇄보정영상을 이용하여 시행하였다. Non-gate, MAG, MTG 영상에서 측정된 종양의 표준섭취계수와 Z축 방향의 크기 그리고 반치폭을 분석하였다 평균 $SUV_{max}$와 $SUV_{peak}$는 NG 대비 MAG 13.15%(P<0.0001), 8.66%(P<0.0001), MTG 13.27%(P<0.0001), 8.80%(P<0.0001) 증가하였으며 Z-축에서 평균 종양의 크기와 반치폭은 MAG 14.47%(P<0.0001), 15.49%(P=0.0004), MTG 14.89%(P<0.0001), 15.79%(P=0.0003) 감소하였으며 통계적으로 유의한 차이를 보였다. MAG와 MTG 비교 평가에서 MTG의 $SUV_{max}$와 $SUV_{peak}$는 MAG 대비 0.07%(P=0.8802), 0.13%(P=0.7766) 증가하였으며 Z-축에서 평균 종양의 크기와 반치폭은 0.49%(P=0.2786), 0.36%(P=0.2488) 감소하였다. 약 7분과 2분의 추가 검사시간이 필요한 MAG와 MTG에서 표준섭취계수와 종양의 크기, 반치폭에서 통계적으로 유의한 차이가 없었다. 간 PET/MRI 검사 시 MR 기반 호흡 움직임 보정 방법을 적용하였을 때 NG 대비 MAG, MTG 모두에서 표준섭취계수와 종양의 크기 및 공간분해능이 개선되었으며 MAG와 MTG의 결과 값은 통계적으로 유의한 차이가 없었다. 호흡에 의한 움직임에 영향을 받는 다양한 상 복부 검사에 MBRMCT를 적용 시 추가적인 장비의 설치 없이 약 2분의 추가 검사시간이 필요한 MTG 방법 적용하여 NG 대비 보다 정확한 정보를 제공할 수 있을 것으로 사료된다.

• 한국동물위생학회지
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• 제36권1호
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• pp.45-52
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• 2013

The purpose of the present study is to determine whether Schisandra chinensis (SC) has a protective effect on high fat diet (HFD)-induced fatty liver including hepatic lipid accumulation in rats. The HFD-induced obese rats were weighed after SC extracts were administered through the gastrointestinal tract at a concentration of 250 mg/kg b.w/day for 5 weeks. After 5 weeks, all of the rats on a high fat-diet were 36.5% heavier compared with normal controls. In contrast, rats on a high-fat diet supplemented with SC were 23.5% lighter than rats fed only a high-fat diet. Although there was no significant difference in food intake among the groups during the experimental diet period, the body weight gain of the SC group was significantly lower than the weights of the HFD groups. SC treatment slightly decreased the liver weight. Reduction of hepatic TBARS contents by SC was observed in rats fed a diet containing SC, and antioxidant activity was markedly increased in HFD＋SC group compared to those of HFD group in liver. Moreover, total-lipid and triglyceride contents in the liver of groups fed a diet containing SC were significantly lower compared to those of the HFD group. High fat feeding elevated liver cholesterol concentration, but the addition of SC to the HFD rats resulted in the significant decrease in liver cholesterol. In histological observation of liver tissues, the hepatocytes of HFD rats showed a typical fatty liver morphology showing numerous lipid droplets in cytoplasm, whereas administration of SC reduced the size and numbers of lipid droplets. These results clearly demonstrated the attenuation of SC on nonalcoholic fatty liver induced by obese rats fed HFD.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.775-780
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• 2014

Objectives: TCR-gamma-delta+T cells (${\gamma}{\delta}$ T cells) are non-conventional T lymphocytes that can recognize and eradicate tumor cells. Our previous studies showed that infiltration and function of ${\gamma}{\delta}$ T cells were substantially attenuated in hepatocellular carcinoma (HCC). However, their prognostic value was not clarified. Methods: The association between ${\gamma}{\delta}$ T cells and the clinical outcomes was determined by immunohistochemistry (IHC) in a HCC patient cohort (n = 342). Results:Immunohistochemistry showed decreased infiltration of ${\gamma}{\delta}$ T cells in tumoral tissues compared with paired peritumoral tissues. The counts of ${\gamma}{\delta}$ T cells in peritumoral tissues were negatively correlated with tumor size (P = 0.005). Survival analysis showed that the levels of peritumoral ${\gamma}{\delta}$T cells were related to both time to recurrence (TTR) and overall survival (OS) (P = 0.010 and P = 0.036, respectively) in univariate analysis, and related to TTR in multivariate analysis (P = 0.014, H.R. [95% CI] = 0.682 [0.502-0.927]). Furthermore, the level of peritumoral ${\gamma}{\delta}$ T cells showed independent prognostic value for TTR in Barcelona Clinic Liver Cancer (BCLC) stage A patients (P = 0.038, H.R. [95% CI] = 0.727 [0.537-0.984]). However, tumoral ${\gamma}{\delta}$ T cells did not show independent prognostic value for either TTR or OS in HCC patients. Conclusions: Low counts of ${\gamma}{\delta}$ T cells in peritumoral liver tissue are related to a higher incidence of recurrence in HCC and can predict postoperative recurrence, especially in those with early-stage HCC.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10713-10718
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• 2015

Purpose: To investigate the prognostic value of serum amyloid A (SAA) in patients with hepatocellular carcinoma (HCC) undergoing surgery. Materials and Methods: Preoperative serum samples of 328 patients with HCC who underwent curative resection and of 47 patients with benign liver lesion were assayed. Serum levels of SAA were measured by enzyme-linked immunosorbent assay and its correlations with clinicopathological characteristics and survival were explored. Results: Levels of SAA were significantly higher in patients with HCC than those with benign liver lesion. There were strong correlations between preoperative serum SAA level and tumor size and more advanced BCLC stage. On univariate analysis, elevated SAA was associated with reduced disease-free survival and overall survival (p=0.001 and 0.03, respectively). Multivariate analyses showed that serum SAA level was an independent prognostic factor for overall survival (hazard ratio 2.80, p=0.01). Conclusions: High SAA serum level is a novel biomarker for the prognosis of HCC patients.

• 정보처리학회논문지B
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• 제12B권5호
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• pp.553-560
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• 2005

일반적으로 간경변에 동반된 비장은 비대한 것으로 알려져 있다. 본 연구는 간경변을 보유한 환자의 복부 CT 영상을 이용하여 비장의 웨이브 패턴(wave patten)을 관찰하였는데 정상간을 보유한 사람의 복부 CT 영상과 차이가 있음을 발견하였다. 간경변을 가지고 있는 환자의 복부 CT 영상에서 비장은 왼쪽 측면에 깊은 웨이브 부분이 존재하였다. 정상간의 경우에도 왼쪽 측면에 웨이브가 존재하기도 하나 깊이가 깊지 않음을 알 수 있다. 그러므로, 간경변을 가진 비장의 웨이브 부분들의 면적의 합이 정상간을 가진 비장의 웨이브 면적보다 크다는 것을 알 수 있다. 나아가, 간경변을 보유한 영상에서 비장의 웨이브 부분을 추출하여 원형성(circularity)을 살펴보았을 때 정상간에 수반된 비장의 웨이브 부분보다 더 원형에 가깝다는 것을 알 수 있었다. 본 논문은 위와 같이 관찰된 원리를 바탕으로 복부 CT 영상에서 비장의 웨이브 패턴을 이용하여 비장 비대를 효과적으로 진단하는 새로운 방법을 제시한다. 이는 단순히 비장의 크기만을 이용하지 않고 비장의 형태 변화로써 국소적 비장 비대를 자동 판정해 낼 수 있음을 말해주는 것이다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6945-6948
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• 2014

Background: Familial adenomatous polyposis (FAP) is a disease inherited in an autosomal dominant fashion. Most FAP patients develop upper gastrointestinal polyps; especially those in the antrum and duodenum are usually neoplastic. The aim of this study was to evaluate the prevalence of gastroduodenal polyps in Iranian FAP patients. Materials and Methods: 28 patients affected by FAP underwent front-view and side-view endoscopy. Papillary biopsies were performed in all patients. Location of polyps, their number and size, pathology study, patient general information (gender, age, family history of FAP or colorectal cancer and gastroduodenal polyps) were analyzed. Results: Gastric polyps were seen in 39.3 % of patients. Some 72.7% of the affected individuals had fundic gland polyps and 36.36% had hyperplastic polyps. Duodenal adenoma was observed in 25% of patients. While 57% of patients had tubular adenoma with low grade dysplasia, 42.8% showed tubulovillous adenoma with low grade dysplasia. Conclusions: Findings of this study indicated that the prevalence of gastroduodenal polyps in FAP patients is high and dysplasia may be evident in duodenal polyps. Therefore, it appears that routine gastroduodenal endoscopy in FAP patients is necessary.