• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4487-4491
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• 2014

Objective: To explore the clinical manifestations and imaging characteristics of gliomatosis cerebri to raise the awareness and improve its diagnostic accuracy for patients. Materials and Methods: Clinical data, imaging characteristics and pathological examination of 12 patients with GC from Jan., 2008 to Jan., 2012 were analyzed retrospectively. Results: Patients with GC were clinically manifested with headache, vomiting, repeated seizures, fatigue and unstable walking, most of whom had more than 2 lesions involving in parietal lobe, followed by temporal lobe, frontal lobe, periventricular white matter and corpus callosum. Magnetic resonance imaging (MRI) showed diffuse distribution, T1-weighted images (T1WI) with equal and low signals and T2-weighted images (T2WI) with bilateral symmetrical high diffuse signals. There was no reinforcement by enhancement scanning and signals were different in diffusion-weighted images (DWI). The higher the tumor staging, the stronger the signals. Pathological examination showed neuroastrocytoma in which tumor tissues were manifested by infiltrative growth in blood vessels and around neurons. Conclusions: In clinical diagnosis of GC, much attention should be paid to the diffuse distribution of imaging characteristics, incomplete matching between clinical and imaging characteristics and confirmation by combining with histopathological examination.

• BMB Reports
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• 제43권4호
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• pp.291-296
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• 2010

Cyclin-dependent kinase 2 (CDK2) is a member of serine/threonine protein kinases, which initiates the principal transitions of the eukaryotic cell cycle and is a promising target for cancer therapy. The present study was designed to inhibit cdk2 gene expression to induce cell cycle arrest and cell proliferation suppression. Here, we constructed a series of RNA interference (RNAi) plasmids which can successfully express small interference RNA (siRNA) in the transfected human cells. The results showed that the RNAi plasmids containing the coding sequences for siRNAs down-regulated the cdk2 gene expression in human cancer cells at the mRNA and the protein levels. Furthermore, we found that the cell cycle was arrested at G0G1 phases and the cell proliferation was inhibited by different siRNAs. These results demonstrate that suppression of CDK2 activity by RNAi may be an effective strategy for gene therapy in human cancers.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제6권7호
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• pp.1831-1853
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• 2012

By sending periodically short bursts of traffic to reduce legit transmission control protocol (TCP) traffic, the low-rate denial of service (LDoS) attacks are hard to be detected and may endanger covertly a network for a long period. Traditionally, LDoS detecting methods mainly concentrate on the attack stream with feature matching, and only a limited number of attack patterns can be detected off-line with high cost. Recent researches divert focus from the attack stream to the traffic anomalies induced by LDoS attacks, which can detect more kinds of attacks with higher efficiency. However, the limited number of abnormal characteristics and the inadequacy of judgment rules may cause wrong decision in some particular situations. In this paper, we address the problem of detecting LDoS attacks and present a scheme based on the fluctuant features of legit TCP and acknowledgment (ACK) traffic. In the scheme, we define judgment criteria which used to identify LDoS attacks in real time at an optimal detection cost. We evaluate the performance of our strategy in real-world network topologies. Simulations results clearly demonstrate the superiority of the method proposed in detecting LDoS attacks.

• BMB Reports
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• 제49권3호
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• pp.167-172
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• 2016

Kaiso is a Pox Virus and Zinc Finger (POZ-ZF) transcription factor with bi-modal DNA-binding specificity. Here, we demonstrated that Kaiso expression is inversely correlated with glucocorticoid receptor (GR) expression in breast carcinomas. Knockdown of Kaiso increased GR expression, while overexpression of Kaiso inhibited GR expression in breast cancer cells. Furthermore, Kaiso repressed GR proximal promoter-reporter activity in a dose-dependent manner. Remarkably, ChIP experiments demonstrated that endogenous Kaiso was associated with the GR promoter sequence in a methylation-dependent manner. Since glucocorticoids inhibit chemotherapyinduced apoptosis and have been widely used as a co-treatment of patients with breast cancer, we assessed the role of Kasio in GR-mediated anti-apoptotic effects. We found that overexpression of Kaiso attenuated the anti-apoptotic effects of glucocorticoids in breast cancer cells. Our findings suggest that GR is a putative target gene of Kaiso and suggest Kaiso to be a potential therapeutic target in GC-combination chemotherapy in breast cancer.

• Biomolecules & Therapeutics
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• 제25권5호
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• pp.471-481
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• 2017

The canonical transient receptor potential channels (TRPCs) constitute a series of nonselective cation channels with variable degrees of $Ca^{2+}$ selectivity. TRPCs consist of seven mammalian members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7, which are further divided into four subtypes, TRPC1, TRPC2, TRPC4/5, and TRPC3/6/7. These channels take charge of various essential cell functions such as contraction, relaxation, proliferation, and dysfunction. This review, organized into seven main sections, will provide an overview of current knowledge about the underlying pathogenesis of TRPCs in cardio/cerebro-vascular diseases, including hypertension, pulmonary arterial hypertension, cardiac hypertrophy, atherosclerosis, arrhythmia, and cerebrovascular ischemia reperfusion injury. Collectively, TRPCs could become a group of drug targets with important physiological functions for the therapy of human cardio/cerebro-vascular diseases.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.788-794
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• 2014

An extracellular ${\beta}$-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 ${\beta}$-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at $65^{\circ}C$ and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The ${\beta}$-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 ${\beta}$-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.

• Smart Structures and Systems
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• 제22권3호
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• pp.359-368
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• 2018

A method is proposed for the identification of instantaneous frequencies (IFs) in time-varying structures. The proposed method combines a maximum gradient algorithm and a smoothing operation. The maximum gradient algorithm is designed to extract the wavelet ridges of response signals. The smoothing operation, based on a polynomial curve fitting algorithm and a threshold method, is employed to reduce the effects of random noises. To verify the effectiveness and accuracy of the proposed method, a numerical example of a signal with two frequency modulated components is investigated and an experimental test on a steel cable with time-varying tensions is also conducted. The results demonstrate that the proposed method can extract IFs from the noisy multi-component signals and practical response signals successfully. In addition, the proposed method can provide a better IF identification results than the standard synchrosqueezing wavelet transform.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1304-1307
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• 2013

Growth of Microcystis aeruginosa could be inhibited significantly within 24 h by the extracellular substances prepared from Aeromonas sp. strain FM. During the treatment, the concentration of extracellular soluble carbohydrates increased significantly in algal culture. Morphological and ultrastructural changes in M. aeruginosa cells, including breakage of the cell surface, secretion of mucilage, and intracellular disorganization of thylakoids, were observed. HPLC-MS analysis showed that the extracellular substances of Aeromonas sp. strain FM were a mixture of free amino acids, tripeptides, and clavulanate. Among these, the algaelysis effects of lysine and clavulanate were confirmed.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.201-207
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• 2000

The freshwater green alga Chlorococcum sp. grew on NH_4^{+},{\;}NO_3^{-}$, urea, yeast extract, and peptone as the nitrogen source showing similar pattens of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction fo SC was urea. The dffects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were stydied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM phosphate, 1.6 mM sulfate, 170 mM NACl, and $50{\;}\mu\textrm{M}$ iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improned SC prodution by manipulating the nutrient levels in the modified media were descussed.

• 대한수학회보
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• 제54권4호
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• pp.1195-1219
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• 2017

In this paper, the limit behavior of solution for the $Schr{\ddot{o}}dinger$ equation with random dispersion and time-dependent nonlinear loss/gain: $idu+{\frac{1}{{\varepsilon}}}m({\frac{t}{{\varepsilon}^2}}){\partial}_{xx}udt+{\mid}u{\mid}^{2{\sigma}}udt+i{\varepsilon}a(t){\mid}u{\mid}^{2{\sigma}_0}udt=0$ is studied. Combining stochastic Strichartz-type estimates with $L^2$ norm estimates, we first derive the global existence for $L^2$ and $H^1$ solution of the stochastic $Schr{\ddot{o}}dinger$ equation with white noise dispersion and time-dependent loss/gain: $idu+{\Delta}u{\circ}d{\beta}+{\mid}u{\mid}^{2{\sigma}}udt+ia(t){\mid}u{\mid}^{2{\sigma}_0}udt=0$. Secondly, we prove rigorously the global diffusion-approximation limit of the solution for the former as ${\varepsilon}{\rightarrow}0$ in one-dimensional $L^2$ subcritical and critical cases.