Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of a ${\beta}$-Glucosidase from Aspergillus niger and Its Application in the Hydrolysis of Geniposide to Genipin

  • Gong, Guohong (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Zheng, Zhiming (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Liu, Hui (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Wang, Li (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Diao, Jinshan (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Wang, Peng (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences) ;
  • Zhao, Genhai (Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences)
  • Received : 2014.01.27
  • Accepted : 2014.03.08
  • Published : 2014.06.28
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Abstract

An extracellular ${\beta}$-glucosidase from Aspergillus niger Au0847 was purified to homogeneity by precipitation with ammonium sulfate, anion exchange, and gel filtration. The purified protein was composed of two subunits with molecular masses of 110 and 120 kDa. Au0847 ${\beta}$-glucosidase exhibited relatively high thermostability and pH stability, and its highest activity was obtained at $65^{\circ}C$ and pH 4.6, respectively. As a potential metalloprotein, its enzymatic activity was potently stimulated by manganese ion and DTT. The ${\beta}$-glucosidase displayed avid affinity and high catalytic efficiency for geniposide. Au0847 ${\beta}$-glucosidase has potential value as an industrial enzyme for the hydrolysis of geniposide to genipin.

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