• Title/Summary/Keyword: Liquid cell

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Improvement of mechanical properties of bio-concrete using Enterococcus faecalis and Bacillus cereus

  • Alshalif, Abdullah Faisal;Juki, Mohd Irwan;Othman, Norzila;Al-Gheethi, Adel Ali;Khalid, Faisal Sheikh
    • Environmental Engineering Research
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    • v.24 no.4
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    • pp.630-637
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    • 2019
  • The present study aimed to investigate the potential of Enterococcus faecalis (E. faecalis) and Bacillus cereus (B. cereus) in improving the properties of bio-concrete. E. faecalis and B. cereus strains were obtained from fresh urine and an acid mire water at cell concentration of 1.16×1012 and 1.3×1012 cells mL-1, respectively. The bacterial strains were inoculated in a liquid medium into the concrete with 1, 3 and 5% as replacement of water cement ratio (w/c). The ability of E. faecalis and B. cereus cells to accumulate the calcite and the decrement of pores size within bio-concrete was confirmed by SEM and EDX analysis. The results revealed that E. faecalis exhibited high efficiency for increasing of compressive and splitting tensile strength than B. cereus (23 vs. 14.2%, and 13 vs. 8.5%, respectively). These findings indicated that E. faecalis is more applicable in the bio-concrete due to its ability to enhance strength development and reduce water penetration.

CFD Modeling for 300MW Shell-Type One-Stage Entrained Flow Coal Gasifier : Effect of $O_2$/Steam/Coal Ratios, Coal Particle Sizes, and Inlet Angles on the Gasifier Performance (300MW급 Shell형 1단 분류층 석탄 가스화기의 전산수치해석 : 산소/스팀/석탄 주입비, 석탄입자 크기, 주입 노즐 각도가 가스화기 성능에 미치는 영향)

  • Song, Ji-Hoon;Kang, Min-Woong;Seo, Dong-Kyun;Lim, Sung-Jin;Paek, Min-Su;Hwang, Jung-Ho
    • Journal of Hydrogen and New Energy
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    • v.21 no.3
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    • pp.227-240
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    • 2010
  • Coal gasification is heading for a great future as one of the cleanest energy sources, which can produce not only electricity and heat, but also gaseous and liquid fuels from the synthesis. The work focuses on 300MW shell type one-stage entrained flow coal gasifier which is used in the Integrated coal Gasification Combined Cycle(IGCC) plant as a reactor. As constructing an IGCC plant is considerably complicated and expensive compared with a pulverized-coal power plant, it is important to determine optimum design factors and operating conditions using a computational fluid dynamics (CFD) model. In this study, the results of numerical calculations show that $O_2$/Coal ratio, 0.83, Steam/Coal ratio, 0.05, coal particle diameter, $100{\mu}m$, injection angle, $4^{\circ}$ (clockwise) are the most optimum in this research.

Investigation of Thermal Management Parameters of Metal Hydride Based Hydrogen Storage System (금속수소화물 기반 수소저장시스템의 열관리 인자 조사)

  • PARK, CHU SIK;KIM, JONG WON;BAE, KI KWANG;JEONG, SEONG UK;KANG, KYOUNG SOO
    • Journal of Hydrogen and New Energy
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    • v.29 no.3
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    • pp.251-259
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    • 2018
  • Metal hydride based hydrogen storage under moderate temperature and pressure gives the safety advantage over the gas and liquid storage methods. Still solid-state hydrogen storage including metal hydride is below the DOE target level for automotive applications, but it can be adapted to stationary or miliary application reasonably. In order to develop a modular solid state hydrogen storage system that can be applied to a distributed power supply system composed of renewable energy - water electrolysis - fuel cell, the heat transfer and hydrogen storage characteristics of the metal hydride necessary for the module system design were investigated using AB5 type metal hydride, LCN2 ($La_{0.9}Ce_{0.1}Ni_5$). The planetary high energy mill (PHEM) treatment of LCN2 confirmed the initial hydrogen storage activation and hydrogen storage capacity through surface modification of LCN2 material. Expanded natural graphite (ENG) addition to LCN2, and compression molding at 500 atm improved the thermal conductivity of the solid hydrogen storage material.

Isolation and Characterization of a Crude oil-Degrading Strain, Nocardia sp. H 17-1 (원유 분해균주 Nocardis sp. Hl7-1의 분리 및 특성)

  • 이창호;권기석
    • KSBB Journal
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    • v.11 no.6
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    • pp.654-662
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    • 1996
  • Bacterial strains which degrade crude oil were isolated by liquid culture from oil-spilled soil, and four isolates were selected among them. The strain Hl7-1 was finally selected after testing emulsifying activity and oil conversion rate. The strain Hl7-1 was identified as a Nocardia sp. based on the test for morphological, biochemical and physiological characteristics. It appears to be highly specialized for growth on crude oil in minimal salts medium since it showed preference for oil or degradation products as substrates for growth. It was found that it could grow on at least fifteen different hydrocarbons. The optimum cultural and environmental conditions were seeked. Cell growth and emulsification activity as a function of time were also determined. Crude oil degradation and the reduction of product peak was identified by the analysis of remnant oil by gas chromatography after 3 days of cultivation. Approximately 83% of oil were converted into a form no longer extractable by organic solvents.

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Computational Fluid Dynamics Study on Uniform Cooling of Polymer Electrolyte Membrane Fuel Cells by Parallel Multi-pass Serpentine Flow Fields (병렬 사형유로를 채택한 냉각판을 통한 고분자 전해질 연료전지의 균일 냉각에 대한 전산유체역학 해석 연구)

  • Yu, Seung-Ho;Baek, Seung-Man;Nam, Jin-Hyun;Kim, Charn-Jung
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.34 no.10
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    • pp.885-891
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    • 2010
  • Thermal management is important for enhancing the performance and durability of polymer electrolyte membrane fuel cells (PEMFCs) and is taken into account in the design of PEMFCs. In general, cooling pates with circulating liquid coolant (water) are inserted between several unit cells to exhaust the reaction heat from PEMFCs. In this study, computational fluid dynamics (CFD) simulations were performed to characterize the uniform cooling performance of parallel multipass serpentine flow fields (MPSFFs) that were used as coolant flow channels in PEMFCs. The cooling performances of conventional serpentine and parallel flow fields were also evaluated for the purpose of comparison. The CFD results showed that the use of parallel MPSFFs can help reduce the temperature nonuniformity, and thus, can favorably enhance the performance and durability of PEMFCs.

Polyoxyethylene Tocopheryl Ethers; A Series of Novel Surfactants from Tocopherol for Functional Cosmetics (토코페롤에서 유도된 기능성 화장품용의 새로운 계면 활성제)

  • 김영대;김창규
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.18 no.1
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    • pp.1-41
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    • 1992
  • A new and unique class of nonionic surfactants was synthesized by reacting biological a-tocopherol with ethylene oxide for functional cosmetics. The structures were confirmed by Hl-UMR, FT-lR, TLC and elemental analysis. POV and conjugated diene value study for EPO showed POE(n)TE had antioxidative effect similar to tocopheryl acetate Protective effect on cell membrane in photohemolysis of POE(5)TE, POE(10)TE and POE(18)TE were slightly lower than tocopherol but higher than nonoxynol-12, and POE(10)TE had UV absorption power comparable with tocopherol and homosalate. Biological activity of the hydrophobic group of the new surfactants make them unique and different from those of conventional nonionic surfactants Systematic safety evaluations of POE(n)TEs on the skin and eye proved that they are as safe as tocopherol. The results of physicochemical study showed POE(10)TE had the lowest CMC value, POE(18)TE had the maximum surface tension reduction and the highest foam volume and POE(n)TEs had various HLB values by the degree of ethoxylation. The test resul Is of technological and practical applications of these surfactants for cosmetics showed some POE(n)TEs were superior to conventional surfactants. POE(5)TE in W/O emulsions, POE(10)TE and POE(12)TE in O/W emulsions, POE(12)TE in dispersions, POE(18)TE in solubilizations and POE(50)TE in gelations were shown to be excellent which was considered due to the structural characteristic and formation of liquid crystals of POE(n)TEs. By the development and applications of these excel lent multi-functional surfactants, innovative functional cosmetics were successfully formulated.

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Evaluation of Antioxidant Activities and Active Compounds Separated from Water Soluble Extracts of Korean Black Pine Barks

  • Shen, Chang-Zhe;Jun, Hong-Young;Choi, Sung-Ho;Kim, Young-Man;Jung, Eun-Joo;Oh, Gi-Su;Joo, Sung-Jin;Kim, Sung-Hyun;Kim, Il-Kwang
    • Bulletin of the Korean Chemical Society
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    • v.31 no.12
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    • pp.3567-3572
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    • 2010
  • Black pine barks from the southern region of Korea were extracted using pressurized hot water and the water soluble extracts were then separated in a stepwise fashion using a variety of solvents, column chromatography (CC), thin layer chromatography (TLC), and high pressure liquid chromatography (HPLC). The antioxidant activities of each fraction and the active compounds were determined based on the radical scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), reductive potential of ferric ion, and total phenol contents. A DPPH test showed that the half maximal effective concentration ($EC_{50}$ value : $6.59{\pm}0.31\;{\mu}g/mL$) of the ethyl acetate fraction (ca. 0.67%) was almost the same as that of the control compounds and inversely proportional to the value of the total phenol contents. The cell viability of the water extracts was confirmed by methyl thiazol-2-yl-2,5-diphenyl tetrazolium bromide (MTT) with enzyme linked immune sorbent assay (ELISA). Catechin, epicatechin, quercetin and ferulic acid were isolated from the ethyl acetate fraction as active compounds and identified by nuclear magnetic resonance. The antioxidant activity as value of DPPH of each of the separated compounds was lower than the ethyl acetate fraction, and ferulic acid was the lowest among these compounds.

Effect of red ginseng NaturalGEL on skin aging

  • Kim, Ye Hyang;Park, Hye Rim;Cha, So Yoon;Lee, So Hun;Jo, Jung Wung;Go, Jung Nam;Lee, Kang Hyuk;Lee, Su Yeon;Shin, Song Seok
    • Journal of Ginseng Research
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    • v.44 no.1
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    • pp.115-122
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    • 2020
  • Background: In aged skin, degradation of collagen fibers, which occupy the majority of the extracellular matrix in the dermis, and changes of aquaporin 3 (AQP3) and skin constituents, such as hyaluronic acid and ceramide, cause wrinkles and decrease skin moisturization to contribute to dryness and lower elasticity skin. Red ginseng (RG) is used as a cosmetic and food material and is known to protect from UVB-induced cell death, increase skin hydration, prevent wrinkles, and have an antioxidative effect. But, in general, RG used as a material is the soluble liquid portion in the solvent, and the part that is not soluble in the solvent is discarded. Thus, we made the whole RG into microgranulation and dispersed in water to produce gel form for using entire RG, and it was named red ginseng NaturalGEL (RG NGEL). Methods: RG NGEL was investigated for matrix metalloproteinases inhibitory activity, induction of Type I collagen, AQP3, hyaluronan synthetase 2, serine palmitoyl transferase, ceramide synthase 3, and filaggrin expression and compared with RG water extract. Results: RG NGEL reduced the levels of UV-induced matrix metalloproteinases and increased Type I collagen in human fibroblast cells and upregulated AQP3, hyaluronan synthetase 2, serine palmitoyl transferase, ceramide synthase 3, and filaggrin expressions in human keratinocytes compared with RG water extract. Conclusion: RG NGEL has the potential as an effective reagent for antiaging cosmetics to improve wrinkle formation and skin hydration.

A Comparison between Bacterial Cr(VI) Resistance and Cr(VI) Reduction among Environmental Isolates (미생물의 크롬내성과 크롬환원의 상호 비교)

  • 오영숙;최성찬
    • Korean Journal of Microbiology
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    • v.33 no.2
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    • pp.125-130
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    • 1997
  • Microbial reduction of hexava1ent(VI) to trivalent(lII) chromium is regarded as one of the mechanisms that confers resistance to bacteria. In order to verify this hypothesis, we compared Cr(VI) resistance with Cr(VI) reduction among 20 phenotypically distinct environmental isolates from Cr-contaminated and uncontaminated soils. With glucose as an electron donor, Cr(VI) reduction by washed cell suspensions ranged from 0.014 to 0.305 mM Cr(VI) reduced $h^1$. Cr(VI) resistance of the isolates were measured by growth inhibitions on a liquid medium containing 2 mM Cr(VI) based on their decrease of $A_{630}1$ as compared to the controls without Cr(VI). The isolates had a broad range of resistance from no inhibition to 93.4% inhibition of their growth. Upon correlation analysis, there was no significant relationship between those two phenomena. At a population level, a comparison of % resistant viable counts among the Cr-contaminated and uncontaminated soils showed 19.1 % and 0.4% of their total viable counts, respectively. The difference of % resistance between two site,. strongly suggested that the Cr(VI) present in the soils influences natural selection for resistant phenotypes. However, it is unlikely that the Cr(VI) resistance is dependent solely on the reduction as judged by the correlation analysis.

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Effects of N & P Treatment Based on Liquid Organic Materials for Capacitive Deionization(CDI) (축전식 탈염 공정의 액상 유기물에 따른 질소(N) 및 인(P) 처리 특성)

  • Lee, Bo-Ram;Jeong, In-Jo;Park, Soo-Gil
    • Journal of the Korean Electrochemical Society
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    • v.16 no.3
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    • pp.123-128
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    • 2013
  • Organic carbons such as methanol, ethanol, iso-propanol, methoxy ethanol, glucose are added(1, 2, 3%) in the 2000 ppm $NH_3$ and $H_3PO_4$. As vol.%. cyclic voltammetry measurement of the capacity with the addition of organic carbon, the results of $NH_3$ + 3 vol.% Methanol Addition, $H_3PO_4$ + 2 vol.% iso-propanol addition of the increase in capacity was observed. Applying to the CDI Module cell with an addiction of organic carbon is confirm that remove $NH_4$-N and $PO_4$-P in sewage. Namely, the removal efficiency of $NH_3$ was increase of 16.4% during adsorption, 30.4% during desorption and the removal efficiency of $H_3PO_4$ was increase of 63% during adsorption, 54.7% during desorption. Therefore, the result of this research is confirm that effect of the N, P removal and considered that reduction of the operating costs without removing the organic matter in the influent wastewater.