• Mass Spectrometry Letters
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• v.2 no.4
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• pp.88-91
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• 2011

The pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer (SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandem mass spectrometry following solid phase extraction. The stereospecific analysis of amlodipine was performed on an ${\alpha}$-acid glycoprotein (AGP) column using a mobile phase comprising 10 mM ammonium acetate (pH 4.0) and propanol at a flow rate of 0.2 mL/min. This method was used to perform a comparative study of the pharmacokinetics of amlodipine and SK310. The results revealed that the pharmacokinetic profile of S-amlodipine after the administration of SK310 was comparable to that following the administration of the racemic mixture.

• Journal of Environmental Health Sciences
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• v.37 no.6
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• pp.474-481
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• 2011

This study investigated urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) in the urine of smokers and non-smokers by liquid chromatography triple quordrupole tandem mass spectroscopy (LC/MS/MS). Compounds analyzed for urinary biomarkers of PAHs were five mono-hydroxylated PAHs metabolites; 1-naphthol, 2-naphthol, 1-hydroxypyrene(1-OHP), 3-phenanthrol, 2-fluorenol. Urine samples were pretreated by enzymatic hydrolysis and solid phase extraction method. Smokers were composed of 17 men and five women; non-smokers 17 men and 16 women. Smoking increased urinary concentrations of five PAHs metabolites significantly higher than those of nonsmokers. Statistically significant correlations among the five PAHs metabolites were shown. The results suggest that LC/MS/MS technology should be useful in the environmental health discipline.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.29-33
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• 2017

The methanol extract of Rhododendron mucronulatum for. albiflorum (RMFA) stems inhibited aldose reductase (AR) activity. The RMFA fractions obtained by stepwise extraction with solvents of different polarity were tested for AR inhibition in vitro using the lens of a rat. Among them, the ethyl acetate (EtOAc) fraction inhibited AR more than the other fractions. Quercetin (1) from the EtOAc fraction showed a high AR inhibition with $IC_{50}$ of $2.11{\mu}M$. The stems of RMFA contained the highest amount (5.12 mg/g extract) of quercetin. Our results suggest that RMFA, which contained quercetin, could be a useful material for the development of supplementary functional foods.

• Archives of Pharmacal Research
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• v.21 no.2
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• pp.217-222
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• 1998

A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-$\beta$-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1.83) on $5{\mu}m$ octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3).

• Korean Journal of Pharmacognosy
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• v.2 no.1
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• pp.39-40
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• 1971

An alkaloidal component was extracted from the root of Aconitum koreanum R. $R_{AYMOND}$ of the commercial origin. Crude extract was obtained in ca. 2% yield out of dried root with ether-methanol (10:1). White amorphous compound was precipitated out from the HCl solution upon the addition of ammonia water, and crude base was obtained by extraction of its mother liquid with chloroform. This crude base was purified by the alumina-column chromatographic method. A pure alkaloidal component(500mg) was yielded from its crude base. Its pure base comes as needle crystals melting at $250{\sim}252^{\circ}C$ (decomp.), $[\alpha]^{20}_D$ D+9.82 (methanol) and Anal. calcd. for $C_{19}H_{27}O_{8}N$.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.691-693
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• 1999

Resveratrol (trans-3,5,4'-trihydroxystilbene), a phenolic substance present in both grapes and wines, has been reported to have certain pharmacological effects. Using an ethylacetate-phase extraction followed by a high performance liquid chromatographic analysis, the concentration of trans-resveratrol was measured in 9 red wines commercially available in Korea, including the Korean red wine (Noul). Noul red wine with a 1998 vintage had a trans-resveratrol concentration of 3.3 ㎎/l, which was rather higher than the concentrations found in other wines (0.19-2.45 ㎎/l) with the exception of the French wine, J. P. Chenet (3.39 ㎎/l). In addition, the grapes of Vitis labrusca cv. Sheridan cultivated in the Kyungsan area showed a trans-resveratrol content of 6.4 ㎎/㎏.

• Advances in Traditional Medicine
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• v.6 no.1
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• pp.65-67
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• 2006

In this study, a new extraction method was developed and two kinds of flavonoids were extracted from the mature fruit of Vaccinium uliginosum L. of China. These two kinds of flavonoids were analyzed by spectral and identified by high-performance liquid chromatography (HPLC) and UV/Vis. The extract of the fruit was total acid hydrolyzed. TLC chromatography was subsequently employed to identify the hydrolysate. Two kinds of aglycone flavonoids, quercetin and myricetin, were identified. At the same time PC chromatography was used to identify the monomer sugar in the flavonoids and it was verified as glucose. HPLC, UV/Vis, and Mass spectrum analyses revealed that the flavonoids were quercetin 3-monoglucosides and myricetin 3-monoglucosides.

• Journal of environmental and Sanitary engineering
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• v.13 no.1
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• pp.104-111
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• 1998

This study was performed to investigate the contents of preservatives in cosmetic lotions and creams. The 55 kinds of creams and the 45 kinds of lotions were tested, and the 6 kinds of preservatives such as sorbic acid (SA), benzoic acid (BA), methlparaben(MP), ethylparaben(EP), propylparaben(PP) and butylparaben(BP) were determined for domestic and foreign cosmetics by high performance liquid chromatography(HPLC). The recovery rates of methanol extraction and distillation method were respectively the range of 84.82 - 99.62% and 17.47 - 79.91% for the spiking concentration of 1.2% in the cosmetic lotions. Excellent isolation was showed at the wavelength of 230nm for 6 kinds of preservatives. Preservatives were detected for all tested samples and their concentration were not exceeded in cosmetic combination limits. Paraoxybenzoate esters(MP, EP, PP, BP) were used in the 98.0% of samples and not less than 2 kinds of preservatives were used in samples.

• Journal of environmental and Sanitary engineering
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• v.17 no.1
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• pp.52-56
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• 2002

The study was carried out to evaluate the characteristics of biodegradation by Pseudomonas putida G7 in soil column. The reactor system was used to investigate mass transfer of VOCs as Toluene in a column of unsaturated soil. Determination of the fate of VOCs in unsaturated soil is necessary to evaluate the feasibility of natural attenuation as a VOCs remediation strategy. The objective of this study was to develop a mechanistically based mathematical model that would consider the interdependence of VOC transport, microbial activity, and sorptive interactions in a moist, unsaturated soil. Because the focus of the model was on description of natural attenuation, the advective VOCs transport that is induced in engineered remediation processes such as vapor extraction was not considered. It can be concluded that the coefficient for gas liquid mass-transfer was found to be a key parameter controlling the ability of bacteria to VOCs. Finally, it appeared that bioremediation technology of VOCs which are difficult to be decomposed by chemical methods.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.2
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• pp.103-108
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• 1995

Antibiotic and physico-chemical properties of an active compound from actinomycetes isolate G-37, of which the culture filtrate had an inhibitory effect against tobacco mosaic virus(W) infection, were examined. The active compound, which was purified by ethylacetate extraction, silica gel column chromatography, preparative thin layer chromatography, and high performance liquid chromatography, showed strong antibacterial activities especially against Gram-positive bacteria including Bacillus subtillis, Sarcina lutea and Staphylococcus aureus. From the IH-NMR, FAB/RfS, UV spectral data, and physicochemical properties, the active compound of G-37 appears to belong to a peptide antibiotic group. Among the known peptide antibiotics in the antibiotic group, No. 280, A-30912, and Taitomycin showed molecular weights and ultra violet spectrum similar to those of the active compound from G-37, but was not identical to the compound, which suggests that it may be a new peptide antibiotics.