• KSBB Journal
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• 제14권6호
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• pp.677-681
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• 1999

계란의 난황으로부터 유래한 IgY의 분리와 정제과정이 흐름을 정리한 결과, 난황에서의 수용성 단백질의 분리단계가 단백질의 수율을 증가시키는데 가장 큰 영향을 미치는 것으로 판단되었다. 본 연구에서는 자연 침전방법을 통하여 실험하였는데 수율의 증가를 위해서는 원심분리와 같이 수용성 단백질의 양을 많이 얻을 수 있는 방법이 필요하고, 인지질이나 lipoprotein등의 성분의 제거효율을 높이기 위해서는 x-carrageenan등과 같은 natural gum성분의 첨가와 같은 방법이 필요하다. 그러므로 자연침전을 이용하여 수율의 증가측면과 비용의 절감의 효과를 얻을 수 있었다. 보다 효율적인 IgY의 분리를 위해서는 초기 단계에서 수율의 극대화와 불순성분 제거의 최대화가 동시에 요구되고 있다. 전처리 후의 시료를 이용하여 이온교환 크로마토그래피와 gel filtration chromatography를 통해 순도 90% 이상의 IgY를 얻을 수 있었다.

• BMB Reports
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• 제51권10호
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• pp.520-525
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• 2018

Cardiovascular diseases arising from atherosclerosis are the leading causes of mortality and morbidity worldwide. Lipid-lowering agents have been developed in order to treat hypercholesterolemia, a major risk factor for atherosclerosis. However, the prevalence of cardiovascular diseases is increasing, indicating a need to identify novel therapeutic targets and develop new treatment agents. Adenosine receptors (ARs) are emerging as therapeutic targets in asthma, rheumatoid arthritis, cancer, ischemia, and inflammatory diseases. This study assessed whether LJ-1888, a selective antagonist for $A_3$ AR, can inhibit the development of atherosclerosis in apolipoprotein E knock-out ($ApoE^{-/-}$) mice who are fed a western diet. Plaque formation was significantly lower in $ApoE^{-/-}$ mice administered LJ-1888 than in mice not administered LJ-1888, without any associated liver damage. LJ-1888 treatment of $ApoE^{-/-}$ mice prevented western diet-induced hypercholesterolemia by markedly reducing low-density lipoprotein cholesterol levels and significantly increasing high-density lipoprotein cholesterol concentrations. Reduced hypercholesterolemia in $ApoE^{-/-}$ mice administered LJ-1888 was associated with the enhanced expression of genes involved in bile acid biosynthesis. These findings indicate that LJ-1888, a selective antagonist for $A_3$ AR, may be a novel candidate for the treatment of atherosclerosis and hypercholesterolemia.

• 한국임상수의학회지
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• 제29권4호
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• pp.277-282
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• 2012

본 연구는 건강한 개에서 혈장 homocysteine농도가 비만이나 비만 관련인자(예, 지방, 인슐린, 혈당, 글루카곤, fructosamine)들과 어떤 상관관계가 있는지를 확인하기 위해 실시되었다. 이를 위해, 절식시 혈당, 지방지수(예, total triglycerides [TG], total cholesterol [TC], high-density lipoprotein cholesterol [HDL-C]과 low-density lipoprotein cholesterol [LDL-C]), fructosamine, insulin 및 glucagon 농도를 각각 측정하였다. 실험에 사용된 개들은 body condition score (BCS)에 따라 분류하였다. 평균 혈장 homocysteine 농도는 비만한 개 집단이 정상이나 마른 개 집단에 비해 상당히 높았다. 또한 혈장 homocysteine농도는 HDL-C농도 뿐아니라 TG, TC, 혈장 glucagon 및 fructosamine 농도와도 밀접한 상관관계를 가지고 있었다. 반대로 LDL-C 농도, 절식시 혈당농도 및 insulin농도는 아무런 상관관계가 확인되지 않았다. 본 연구 결과, homocysteine의 농도 상승이 비만견에서 생물학적으로 중요한 역할을 하는 것으로 추정된다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1460-1464
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• 2001

This study compared the lipid metabolism of white Leghorn layers between the growing and the laying periods. The study was conducted on thirty layers in the growing period (14 weeks old) and in the initial laying period (36 weeks old; 14 weeks from the onset of laying). Results indicated that all plasma lipid traits were significantly different (p<0.05) between the two periods. The estrogen concentration in the laying period was about 3 fold that in the growing period. Triacylglycerol (TG) concentration in the laying period was about 12 fold that in the growing period. The phospholipids, cholesterol, glycerol and non esterified fatty acid (NEFA) in the laying period were also higher than those in the growing period (p<0.05). Lipogenic enzyme activities in the laying period were higher than in the growing period (p<0.05). High-density lipoprotein (HDL) was the largest lipoprotein portion in growing layers. In laying hens, the major lipoprotein portion was very low-density lipoprotein (VLDL). It was also shown that protein is the primary component of HDL and TG is the largest portion of VLDL in both periods. In laying hens, VLDL contained more TG than that in growing layers (p<0.05). The VLDL particle size in laying hens was larger than that in growing layers (p<0.05). This study indicates that the lipid metabolism traits of a layer in the laying period were different from those in the growing period. The lipogenesis related traits in the laying period were markedly greater than in the growing period.

• 한국식품영양과학회지
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• 제26권1호
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• pp.25-31
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• 1997

갈근(Puerariae radix)을 MeOH 추출물에서 silica gel column chromatography를 이용하여 플라보노이드 화합물인 daidzin 및 puerarin를 분리하여 low density li-poprotein의 산화에 대하여 실험하였다. 이들 플라보노이드중 daidzin의 경우 100$\mu\textrm{g}$/$m\ell$에서 puerarin은 600$\mu\textrm{g}$/$m\ell$의 농도에서 5$\mu$M Cu$^{2+}$ 매개산화 LDL에 대하여 억제 효과가 좋았다. 이때 같은 농도의 diadzin과 puerarin를 첨가한 산화 LDL의 전기영동의 이동거리는 native LDL 보다는 약간 높았으나 oxidized LDL의 대조군 보다는 이동거리가 낮았다. 또 J774 및 macrophages 유도 산화 LDL에 있어서도 100$\mu\textrm{g}$ daidzin과 600$\mu\textrm{g}$/$m\ell$ puerarin을 첨가 하였을 때 억제효과 나타났다. LDL을 5$\mu$M Cu$^{2+}$존재하에서 산화시킬 때 동일 농도의 daidzin과 puerarin 을 첨가하면 conjugated dienes의 생성이 거의 억제되었다.

• 한국식품영양과학회지
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• 제21권5호
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• pp.490-495
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• 1992

본 실험에서는 혈장 lipoprotein의 콜레스테롤 및 지질에 대한 비타민 E의 하강효과를 비타민 E 비급이 및 비타민 E 급이(700IU/kg diet)와 콜레스테롤 급이(2%/kg diet)의 순서를 바꾼 경우와 양자를 동시에 급이한 4개 실험군으로 나누어 비교분석하였다. 비타민 E의 콜레스테롤 및 지질하강효과는 비타민 E 급이시 높았으며 특히, 콜레스테롤 급이후에 따른 비타민 E의 급이효과가 더욱 뚜렷하엿다. 이러한 효과는 혈장 lipoprotein내 콜레스테롤과 비타민 E의 농도비를 비교함으로서도 분명하였다. 그러나 콜레스테롤과 비타민 E의 동시급이인 경우에는 콜레스테롤 흡수에 대한 비타민 E의 상승효과를 보였다. HDL/LDL 콜레스테롤의 상관비에 대한 비타민 E의 효과 역시 충분한 비타민 E의 존재하에서 높아짐을 보였다.

• Mycobiology
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• 제39권4호
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• pp.283-289
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• 2011

The wild edible mushroom, Lentinus lepideus has recently been cultivated for commercial use in Korea. While the mushroom has been widely used for nutritional and medicinal purposes, the possible anti-hyperlipidemic action is unclear. The effects of dietary L. lepideus on plasma and feces biochemical and on the liver histological status were investigated in hypercholesterolemic rats. Six-wk-old female Sprague-Dawley albino rats were divided into three groups of 10 rats each. Biochemical and histological examinations were performed. A diet containing 5% L. lepideus fruiting bodies reduced plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, phospholipids, and the ratio of low-density to high-density lipoprotein. Body weight was reduced. The diet did not adversely affect plasma biochemical and enzyme profiles. L. lepideus reduced significantly plasma ${\beta}$- and pre-${\beta}$-lipoprotein, while ${\alpha}$-lipoprotein content was increased. A histological study of hepatic cells by conventional hematoxylin-eosin and oil red O staining revealed normal findings for mushroom-fed hypercholesterolemic rats. The present study suggests that a diet supplemented with L. lepideus can provide health benefits by acting on the atherogenic lipid profile in hypercholesterolemic rats.

• Journal of Ginseng Research
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• 제36권2호
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• pp.169-175
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• 2012

Ginseng has been used as a traditional medicine for treatment of many diseases and for general health maintenance in people of all ages. Ginseng is also used to ameliorate menopausal systems. We investigated the estrogenic activity of Korean red ginseng (KRG) in a transient transfection system, using estrogen receptor (ER) and estrogen-responsive luciferase plasmids in MCF-7 cells. The extract activated both ER${\alpha}$ and ER${\beta}$. KRG modulated the mRNA levels of estrogen-responsive genes such as pS2 and ESR1 and decreased the protein level of ER${\alpha}$. In order to examine in vivo estrogenic activity of KRG, sixteen female Sprague-Dawley rats separated into four groups were studied for nine weeks: non-ovariectomized (OVX) rats treated with olive oil, OVX rats treated with olive oil, OVX rats treated with 17-${\beta}$-estradiol (E2) in olive oil, and OVX rats treated with KRG extract in olive oil. The experiments were repeated for three times and the data of twelve rats were combined. Body weight of OVX rats was greater than that of sham-operated control rats and was decreased by E2 treatment. Uterine weight increased after E2 treatment compared to OVX rats. However, no difference in body or uterine weight was observed with KRG intake. KRG induced reductions in total cholesterol, low density lipoprotein cholesterol/total cholesterol, high density lipoprotein cholesterol/total cholesterol, and low density lipoprotein cholesterol/high density lipoprotein cholesterol, but not to the same degree as did E2 intake. These results show that KRG does contain estrogenic activity as manifested by in vitro study but the activity is not strong enough to elicit physiological responses.

• Journal of Nutrition and Health
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• 제35권10호
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• pp.1023-1030
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• 2002

This study was conducted to examine the effects of dietary xylooligosaccharides on lipoprotein lipase (LPL) activitiy in epididymal adipose tissue and lipid composition in serum of rats fed normal or high fat diet. Male Sprague-Dawley male rats weighing 100 $\pm$ 10 g were randomly divided into four groups, two normal diets and two high fat diets containing 1% cholesterol and 10% lard. Two normal diets were classified into a basal diet (normal group) and that with 10% xylooligosaccharide diet (NX group). The high fat diet groups were classified into a HF group without xylooligosaccharides diet and HFX group supplemented with 10% xylooligosacchride diet. Experimental diets were fed ad libitum to the rats for 4 weeks and then they were sacrificed. Body weight, epididymal weight and abdominal weight in HF group were hevier than the those of normal group, but HFX group was significantly reduced compared to HF group. Relative body weight to epididymal weight and relative body weight to abdominal weight in HF group were increased to 50%, 51%, respectively, compared to normal group, but HFX group was reduced 22%, 16%, respectively, compared to HF group. The levels of serum triglyceride, total cholesterol, LDL-cholesterol and atherogenic index in HFX groups were significantly lower than those of HF group, whereas HDL-cholesterol levels were significant increased. Triglyceride contents of epididymal adipose tissue in HF group was increased to 39%, compared to normal group, but HFX group was reduced to 15.8%, compared to HF group. Cholesterol contents of epididymal adipose tissue in HF group was increased 121%, compared to normal group, but HFX group was reduced to 26%, compared to HF group. The activity of LPL in epididymal adipose tissue was increased to 259% in HF group, compared to normal group and HFX group was reduced to 66%, compared to HF group. These result of this study suggested that improved lipid metabolism observed in rats fed xylooligosaccharides may be caused by an alteration of LPL activity in epididymal adipose tissue and lipid composition in serum.

• Nutrition Research and Practice
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• 제11권4호
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• pp.327-333
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• 2017

BACKGROUND/OBJECTIVES: Complications of diabetes, such as cardiovascular disease, are associated with increased mortality among type 2 diabetes mellitus patients. Homocysteine has been recently identified as a predictor of cardiovascular disease-related complications in diabetes. We investigated whether or not supplementation with folic acid tablets can lower homocysteine levels and improve parameters related with vascular complications. SUBJECTS/METHODS: We conducted a non-randomized 8-week trial involving postmenopausal diabetic women (n = 25) supplemented with $800{\mu}g$ of folic acid ($400{\mu}g$ twice a day) daily. Subjects' serum levels of folate, homocysteine, and vitamin $B_{12}$ were measured, along with vascular function and brachial-ankle pulse wave velocity. RESULTS: Folic acid supplementation significantly increased serum folate levels (P < 0.0001), reduced homocysteine levels (P < 0.0001), and increased vitamin $B_{12}$ levels (P = 0.0063). There were significant decreases in low-density lipoprotein cholesterol levels as well as the ratios of low-density lipoprotein cholesterol to high-density lipoprotein cholesterol and total cholesterol to high-density lipoprotein cholesterol. Brachial-ankle pulse wave velocities were not altered by supplementation. Changes in serum vitamin $B_{12}$ after folic acid supplementation were negatively correlated with changes in brachial-ankle pulse wave velocity. CONCLUSIONS: In this study on postmenopausal Korean women with type 2 diabetes mellitus, folic acid supplementation reduced serum homocysteine levels, increased serum folate and vitamin $B_{12}$ levels, and lowered lipid parameters.