• Korean Journal of Pharmacognosy
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• v.9 no.4
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• pp.157-167
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• 1978

Atherosclerosis is associated with the presence of extracellular lipid droplets and large fatty deposits, both of which are to be covered at the surface mainly by zwitterionic phospholipids. The development of atherosclerosis is often associated with the accumulation of calcium. Furthermore, the presence of glycosaminoglycans directly underlying fatty deposits in human aorta has been demonstrated. Also, the possible involvement of the interaction between sulfated polysaccharide and lipoprotein in the development of atherosclerosis has been suggested in view of the presence of both low density lipoproteins and glycosaminoglycans, as well as their complexes, in atherosclerotic aortas. Therefore interactions of sulfated polysaccharides with low density lipoproteins which serve as a vehicle for cholesterol and cholesterol ester and with zwitterionic phospholipids have been studied extensively by a number of workers to provide mechanisms. In this paper, the mechanism of the interaction of sulfated polysaccharides with low density lipoproteins and the mechanism of the interaction between sulfated polysaccharides and zwitterionic phospholipids are reviewed. The possibility of the occurence of these interactions in the body are also considered.

• Korean Journal of Veterinary Research
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• v.20 no.2
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• pp.143-150
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• 1980

The light and electron microscopic observations were carried out in order to know the morphological changes of the fat-storing cells (FSC) in the liver of rabbits given Ginseng for 30 days. The results obtained were as follows: The FSC of experimental group was shown the appearance of a few large lipid droplets, enlarged rough surfaced endoplasmic reticulum, some residual body in the enlarged rER and the nuclear body in some cases. As these findings, it could be suggested that the Ginseng affect the synthesis and accumulation of lipid on the FSC.

• Korean Journal of Veterinary Research
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• v.15 no.2
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• pp.241-250
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• 1975

The light and electron microscopic observations were carried out in order to know the morphological changes of the testicular Leydig cells in the mouse injected with testosterone propionate for a long period. The results obtained were as follows: With the light microscopic study, atrophy of the Leydig cells and larger sudanophilic lipid granules in the experimental group than normal were observed. 2. By the electron microscopic finding, the small spherical or oval mitochondria, large lipid droplets, a decrease in number of smooth endoplasmic reticutum and distended saccular or vacuolar smooth endoplasmic reticulum were observed in the experimental group. Membranous whorls with droplet increased in number and size in the experimental group.

• Medical Lasers
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• v.10 no.4
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• pp.246-249
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• 2021

The carbon dioxide (CO₂) laser is one of the treatment options used for xanthelasma palpebrarum. However, even if the full dermal layer is removed, the lesion can recur due to the residual lipid deposits. A 44-year-old male patient with xanthelasma on both upper eyelids was treated with a pulsed dye CO₂ laser. On the right upper eyelid, we carried out a CO₂ laser treatment until the yellowish plaque was almost invisible and the full thickness of the dermal layer was removed. On the left upper eyelid, the dermal layer was partially removed and an additional squeezing out of yellowish particles was done. The lesion treated by the squeezing out of lipid droplets showed better long-term results than the lesion treated up to the deeper dermal layer.

• Nutrition Research and Practice
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• v.12 no.4
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• pp.298-306
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• 2018

BACKGROUND/OBJECTIVES: Obesity is a global health problem of significant importance which increases mortality. In place of anti-obesity drugs, natural products are being developed as alternative therapeutic materials. In this study, we investigated the effect of Brassica juncea L. leaf extract (BLE) on fat deposition and lipid profiles in high-fat, high-cholesterol diet (HFC)-induced obese rats. MATERIALS/METHODS: Male Sprague-Dawley rats were divided into four groups (n = 8 per group) according to diet: normal diet group (ND), high-fat/high-cholesterol diet group (HFC), HFC with 3% BLE diet group (HFC-A1), and HFC with 5% BLE diet group (HFC-A2). Each group was fed for 6 weeks. Rat body and adipose tissue weights, serum biochemical parameters, and tissue lipid contents were determined. The expression levels of mRNA and proteins involved in lipid and cholesterol metabolism were determined by reverse transcription polymerase chain reaction and western blot analysis, respectively. RESULTS: The HFC-A2 group showed significantly lower body weight gain and food efficiency ratio than the HFC group. BLE supplementation caused mesenteric, epididymal, and total adipose tissue weights to decrease. The serum levels of triglyceride, total cholesterol, and low-density lipoprotein cholesterol were significantly reduced, and high-density lipoprotein cholesterol was significantly increased in rats fed BLE. These results were related to lower glucose-6-phosphate dehydrogenase, acetyl-coA carboxylase, and fatty acid synthase mRNA expression, and to higher expression of the cholesterol $7{\alpha}$-hydroxylase and low density lipoprotein-receptor, as well as increased protein levels of peroxisome proliferator-activated receptor ${\alpha}$. Histological analysis of the liver revealed decreased lipid droplets in HFC rats treated with BLE. CONCLUSIONS: Supplementation of HFC with 3% or 5% BLE inhibited body fat accumulation, improved lipid profiles, and modulated lipogenesis- and cholesterol metabolism-related gene and protein expression.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.4
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• pp.608-613
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• 2011

The aqueous Green tea comes to be used with the Oriental medicine plant, it has the numerous health benefits, including antioxidant, anti-inflammatory, and anti- carcinogenic properties. Epidermal progenitor cells give rise to multiple skin lineages: hair follicle, sebaceous gland and the overlying interfollicular epidermis. Sebocytes are the cells of the sebaceous gland, which synthesize and accumulate lipid dropolets. In order to determine the effect of Green tea on lipid production, several experiments were performed in SZ95 cells (sebocytes). We found that Green tea increased lipid droplets compared with control in a dose-dependent manner. Human sebaceous glands produce sebum, a lipid mixture of squalene, wax esters, triglycerides, cholesterol esters, and free fatty acids that is secreted onto the skin. Therefore, to investigate the effects of Green tea on intracellular lipid levels, we treated SZ95 cells with Green tea, and then examined cholesterol and triglyceride levels. After treatment of the cells with Green tea, the cholesterol and triglyceride levels of SZ95 cells were increased significantly in a dose-dependent manner.

• Animal Bioscience
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• v.35 no.2
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• pp.155-165
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• 2022

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1589-1593
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• 2005

The aim of this study was to investigate the effects of testosterone, 17$\beta$-estradiol, and progesterone on the differentiation of bovine intramuscular adipocytes (BIA). Stromal-vascular (SV) cells were obtained from M. longissimus dorsi of 20 months old Korean (Hanwoo) steers, and were cultured in DMEM containing 5% FBS. The proliferated BIA were induced to differentiate with 0.25 $\mu$M dexamethasone, 0.5 mM 1-methyl-3-isobutyl-xanthine and 10 $\mu$g/ml insulin. During differentiation, the cells were treated with testosterone, 17$\beta$-estradiol, and progesterone at concentrations of $10^{-10}$, $10^{-9}$, and $10^{-8}$ M, respectively, for 12 days. Regardless of its concentration, testosterone remarkably reduced lipid droplets in the cytosol of BIA. On the other hand, 17$\beta$-estradiol and progesterone increased the accumulation of lipid droplets in BIA. Testosterone significantly (p<0.05) decreased GPDH activities with a dose-dependent pattern. 17$\beta$-Estradiol treatment onto BIA during differentiation, however, increased GPDH activity showing the highest activity (11.3 nmol/mg protein/min) at $10^{-10}$ M. Treatment of BIA with progesterone also increased (p<0.05) GPDH activity with the highest activity (13.8 nmol/mg protein/min) at $10^{-9}$ M. In conclusion, the results in the current study suggest that testosterone inhibits differentiation of BIA by suppressing GPDH activity while 17$\beta$-estradiol and progesterone have adverse effects.

• Applied Microscopy
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• v.14 no.2
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• pp.29-37
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• 1984

Morphological changes of the epithelium of the endometrium by prolonged treatment of $17{\beta}$-estradiol or progesterone in ovariectomized rats was studied at the ultrastructural level. The epithelium of the endometrium in ovariectomized rats was characterized by the appearance of a number of vacuoles which was contained with the membraneous structures, lipid droplets and the others. The epithelium was low cuboidal, and a few short microvilli were present at the cell surface. Secretory granules are rarely found. After estradiol treatment, the epithelium was high columnar in shape. The mitochondria was appeared throughout the cytoplasm, however, long or swelling mitochondria was often found. Golgi apparatus and rER were relatively well-developed. Relatively long and sparse microvilli were present at the cell surface. After progesterone treatment, the epithelium was characterized by the appearance of numerous vesicles at the apical region and numerous lipid droplets at the subnuclear region. At the cell surface a number of short and blunt microvilli were found. These data indicated that the endometrium was dependent on estrogen and progesterone for changes in both its morphological and functional state and suggested that each hormone exerted a unique effect on the epithelial cells.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.473-478
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• 1986

In order to elucidate the patterns of natural oils and fats assimilation by microorganisms, lipases properties of yeast and bacterium strain, Torulopsis candia Y-128 and Acinetobacter calcoaceticus KB-2, which could assimilate palm oil efficiently, were investigated. T candida Y-128 attached palm oil droplets directly, and assimilated unsaturated fatty acid more easily than saturated acids liberated by the action of its lipase. Lipase of A. calcoaceticus KB-2 was extracellular and appeared quickly from the beginning of log phase of growth, whereas lipase of f candida Y-128 appealed intracellular. The lipases of two strains seem to be only enough to utilize the lipid materials for their own growth, without accumulation of lipases in the culture broth. Lipases of the strains have 1 (3-)-positional specificities on triglycerides. The patterns of palm oil assimilation showed that two strains attached droplets of lipid materials directly and split off fatty acids at 1 (3-)-position of triglycerides first, and assimilated the reaction products via fatty acids metabolic pathway.