• 대한화학회지
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• 제38권8호
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• pp.608-615
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• 1994

양식산 새우(P.orientalis)로부터 0.05M 인산염 완충용액(pH 7.0)으로 carotenoprotein을 추출하고 DEAE-셀룰로오스, 반포화 황산암모늄 친전 및 GPC로 $\alpha$-form과 ${\beta}$-form 두 종류의 carotenoprotein을 분리 정제하였다. 최대 흡수파장은 ${\lambda}_{max}$= 480, 409, 318 및 280 nm였다. SEM과 XRD로 분자구조를 관찰하였으며, GPC와 PAGE로 측정한 분자량은 $\alpha$-form이 170KDa으로 분리되는 불균일한 이합체 구조였다. carotenoprotein 복합체의 아미노산, 중성지질, 인지질 및 유리지방산 조성을 분석하였고, 여러 가지 유기화학 반응과 UV/Vis,IR, $^1H$-NMR, MS 등으로 carotenoids분자구조를 측정한 결과 lipid-protein의 보호그룹으로서는 astaxanthin과 그 ester임이 확인되었다.

• Bulletin of the Korean Chemical Society
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• 제33권7호
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• pp.2187-2192
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• 2012

This study presents a facile way synthesizing disc-like lipid bilyaer nanostructures with a cationic amphiphilic polyelectrolyte. The cationic amphiphilic polyelectrolyte was in a form of partially quarternized copolymer and was synthesized with 2-(dimethylamino)ethyl methacrylate and stearyl methacrylate. At some concentration ranges of the polymer, the addition of the polymer to lipid components during the preparation of bilayer nanostructures resulted in discs with a fairly high yield (~99%). The mechanism for the formation of the nanostructures was discussed based on the physical properties of these nanostructures and by comparing the nanostructures obtained with an anionic amphiphilic polyelectrolyte.

• 대한한방내과학회지
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• 제17권1호
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• pp.265-277
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• 1996

Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free radical generating enzyme and lipid peroxidation in rat's kidney. In vitro, levels of lipid peroxide in tissues of brain were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron (Fe+2), In vivo, after both herbs were administered to the rat, levels of lipid peroxide in kidney were decreased only in Jwagyuyeum. The enzyme activities and the ratio of type conversion of xanthine oxidase were decreased only in Jwagyuyeum. But, We can't see special changes in Woogyuyeum. The enzyme activities of aldehyde oxidase was lowered in both herbs, especially Jwagyuyeum was much more done. These results suggest that Jwagyuyeum and Woogyuyeum decrease the activities of free radical generating enzymes such as xanthine oxidase and aldehyde oxidase which form lipid peroxide.

• Macromolecular Research
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• 제15권6호
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• pp.547-552
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• 2007

Water soluble polymer, poly(vinyl pyrrolidone) was chosen to conjugate with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(succinyl) (N-succinyl DPPE) to make a new drug delivery system. PVP with an amine group (amino-PVP) was polymerized by free radical polymerization. The amine group of amino-PVP was conjugated with the carboxylic group of N-succinyl DPPE. The resultant conjugate could form nanoparticles in the aqueous solution; these nanoparticles were termed a lipid-polymer system. The critical aggregation concentration was measured with pyrene to give a value of $1{\times}10^{-3}g/L$. The particle size of the lipid-polymer system, as measured by DLS, AFM and TEM, was about 70 nm. Lipophilic component in the inner part of the lipid-polymer system could derive the physical interaction with hydrophobic drugs. Griseofulvin was used as a model drug in this study. The loading efficiency and release profile of the drug were measured by HPLC. The loading efficiency was about 54%. The release behavior was sustained for a prolonged time of 12 days. The proposed lipid-polymer system with biodegradable and biocompatible properties has promising potential as a passive-targeting drug delivery carrier because of its small particle size.

• 대한한의학회지
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• 제16권2호
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• pp.348-364
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• 1995

Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free radical generating enzyme and lipid peroxidation in senile rat brain. In vitro, levels of lipid peroxide in tissues of brain were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron ($Fe^{-2}$). In vivo, after both berbs were administered to the rat. levels of lipid peroxide in brain were decreased. especially it was much more decreased using Jwagyuyeum. Also, enzyme activities of xanthine oxidase and aldehyde oxidase in brain were decreased. The ratio of type conversion of the brain xanthine oxidase was lowered in both, especially Jwagyuyeum was much more done. These results suggest that Jwagyuyeum and Woogyuyeum decrease the activities of free radical generating enzymes such as xanthine oxidase and aldehyde oxidase which form lipid peroxide, Consequently both herbs might delay aging.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1998년도 학술발표회
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• pp.34-34
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• 1998

Tolaasin is a channel forming bacterial toxin produced by Pseudomonas tolaasii and causes a brown blotch disease on cultivated oyster mushrooms. When tolaasin molecules form channels in the membranes of mushroom cells, they destroy cellular membrane structure, known as 'colloid osmotic lysis'. In order to understand the molecular mechanisms forming membrane channels by tolaasin molecules, we have investigated the electrophysiological characteristics of tolaasin-induced channels in lipid bilayer.(omitted)

• 공업화학
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• 제24권2호
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• pp.132-137
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• 2013

포스파티딜콜린이 다량 함유된 수첨레시틴을 이용한 실험에서 액정구조가 잘 형성됨을 알 수 있었다. 액정구조를 형성하는 물질로는 포스포리피드, 에탄올, 물로 이루어짐을 알 수 있었고 로즈마린산을 지표물질로 캡슐화하였다. 액정의 평균입자크기는 480 nm ~ $3{\mu}m$로 이는 액의 구성물질과 물리적인 힘에 따라 다양한 크기의 형태로 존재하였다. 리포좀과 비교했을 때 액정구조는 매우 높은 캡슐화 효율을 보였다. 수첨레시틴의 함량이 증가하면 많은 액정을 형성하였고, 수첨레시틴의 함량이 적으면 액정의 양도 적게 나타났다. 로즈마린산을 함유한 액정구조의 방출실험결과 리포좀으로 형성된 베지클보다 훨씬 적은 양이 방출됨을 알 수 있었다.

• 한국잠사곤충학회지
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• 제33권2호
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• pp.100-102
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• 1991

제사공정의 부잠사 처리폐수에 대하여 그 성상과 추출 유지성분을 분석한 결과 1. 부잠폐수의 COD 605mgO/$\ell$ 총 용존 고형물량 2,335mg/$\ell$ 총 부유 고형물량 2,234mg/$\ell$이었다. 2. 부잠폐수의 지질에는 triglyceride가 76.8%로 가장 많이 함유되었으며 유리 지방산 12.5%와 diglyceride 5.7% free sterol 5.0% 순이었다. 3. 부잠폐수지질의 지방산 조성은 oleic acid, linolenic acid와 linoleic acid의 함유율이 64.93%로 가장 많이 함유되어 있고 palmitic acid 29.39% stearic acid 4.93%의 순이었다.

• Journal of Pharmaceutical Investigation
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• 제31권3호
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• pp.167-172
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• 2001

All-trans retinoic acid (ATRA), vitamin A acid, has been shown to exert anticancer activity in a number of types of cancers, particularly in acute promyelocytic leukaemia (APL). Due to its highly variable bioavailability and induction of its own metabolism after oral treatment, development of parenteral dosage forms are required. However, its poor aqueous solubility and chemical unstability give major drawbacks in parenteral administration. This study was undertaken to investigate a possibility to develop a parenteral formulation of ATRA by employing solid lipid nanoparticle (SLN) as a carrier. By optimizing the production parameters and the composition of SLNs, SLNs with desired mean particle size (<100 nm) as a parenteral dosage form could be produced from trimyristin (as solid lipid), Egg phosphatidylcholine and Tween 80 (as SLN stabilizer). The mean particle size of SLN formulation of ATRA was not changed during storage, suggesting its physical stability. Thermal analysis confirmed that the inner lipid core of SLNs exist at solid state. The mean particle size of ATRA-loaded SLNs was not significantly changed by the lyophilization process. ATRA could be efficiently loaded in SLNs, while maintaining its anticancer activity against HL-60, a well-known APL cell line. Furthermore, by lyophilization, ATRA loaded in SLN could be retained chemically stable during storage. Taken together, our present study demonstrates that physically and chemically stable ATRA formulation adequate for parenteral administration could be obtained by employing SLN technology.

• Journal of Nutrition and Health
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• 제37권8호
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• pp.662-668
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• 2004

This study investigated the effects of soy oligosaccharide consumption on feces bifidobacteria proliferation and feces lipid profiles in Korean young women. Eight healthy young women (25 - 34 years) were fed 15 g/day of soyoligosaccharide solution, containing 3 g of oligosaccharide as form of raffinose and starchyose, for 15 days with their habitual meals. Soyoligosaccharde intake increased the numbers of fecal total bacteria significantly until 10 days (p < 0.05) and the numbers of fecal bifidobactreia were significantly increased until 15 days (p < 0.05) . The fecal pH was significantly decreased (p < 0.05) by soyligosaccharide intake. Fecal lipid concentration showed the trend to increse, especially fecal triglyceride level was significantly increased by soy oligosaccharide intake (p < 0.05). The water contents of feces, the amount of feces, evacuation frequency and taking time to evacuation were not affected by soyoligosaccharide intake. The color of feces changed to yellow-brown, and hardness of stool and effort to evacuation were reduced by soyoligosaccharide intake. These results suggest that soyoligosaccharide intake (3 g/day) in young women improved the gut microflora and fecal lipid profile. Therefore, soy oligosaccharide has a potential to be used as one of the promising prebiotics, and controlled trials with larger sample sizes and longer duration are need to be studied further.