• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.193-198
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• 2012

This study was to examine expression of the recombinant full-length adiponectin (recombinant adiponectin) in insect ovarian cell culture system and to characterize structural properties of the recombinant adiponectin secreted in medium. Gene construct encoding the recombinant adiponectin contained N-terminal collagen-like domain (110 Amino Acids, AAs), C-terminal globular domain (137 AAs) and C-terminal peptides for detection with V5 antibody (26 AAs included adaptor peptide) and purification using the 6xHis tag (6 AAs). The approximate molecular weight of the product (monomer) was 35 kDa. Molecular mass species of the expressed recombinant adiponectin were monomer (~35 kDa), dimer (~70 kDa), trimer (~105 kDa) and hexamer (~210 kDa). The major secreted species were the LMW forms, such as monomer, dimer, and trimer. There was MMW of hexamer as minor form. HMW multimers (~300 kDa) were shown as a tracer or not detected on the SDS-PAGE in several experiments (data not shown). The multimer forms in this study were not compatible to those in animal or human serum and adipose tissue by other researcher's study in which the major multimer forms were HMW. By protein denaturing experiments with reducing reagent (${\beta}$-MeOH), anionic detergent (SDS) and heat ($95^{\circ}C$) on the SDS-PAGE, not all adiponectin multimers seemed to have disulfide bond linked structure to form multimers. The recombinant adiponectin which expressed in insect ovarian cell culture system seemed to have the limitation as full physiological regulator for the application to animal and human study.

• Journal of Korea Port Economic Association
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• v.17 no.2
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• pp.111-137
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• 2001

The establishment of the WTO system means that the global age of trade has officially arrived. Since the integration of the world economy brings about the free movement of goods and services between nations, it is inevitable that sea-bound freight will continue to increase. A recent World Bank report says that China and Korea will be the first and seventh largest economic Powers, respectively, by the year 2020. In particular, the Korea peninsula has a geo-political advantage in being developed as a major Northeast Asian container center. Moreover China's swift uprising needs new order of trade for economy belt in Northeast Asia. Therefore, it can be said that Kunsan Port. which has already been designated as a free trade zone, has greater potential to rise as a regional beach-head port and main region for foreign investment. As such. Kunsan Port will play a major role in accelerating the emergence of the West Sea in international trade. There are several strategies for developing Kunsan Port into a central container port: 1) develop Kunsan port Into an import/export front base f3r multinational corporations, 2) develop Kunsan container port into the core composite container-linked central port, 3) attract foreign investment to the Kunsan Free Trade Area so as to promote balanced development among the regions of Korea, 4) support the central government and local governments to accelerate the coming of the "West Sea Era." These recommendations call for urgent implementation.

• Journal of Korean Academy of Nursing Administration
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• v.19 no.2
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• pp.292-303
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• 2013

Purpose: The purpose of this study was to explore nurses' experience of evidence-based nursing practice in general hospitals. Methods: Data were collected from 13 nurses through in-depth interviews about their experiences with evidence-based practice. The research questions were "What kind of evidence are you using in your practice?" and "What are the barriers to and enablers of evidence-based practice that you have experienced?" Qualitative data from field and transcribed notes were analyzed using qualitative content analysis methodology. Results: Major themes of using evidence were identified as 'research as primary valid evidence', 'information from local context and internet as realistic evidence', and 'clinical experience as pragmatic evidence'. Patient experience was not used as evidence in solving nursing problems. Barriers to and enablers of evidence-based practice were linked. They included both external, organizational factors and individual factors. Main issues were 'lack of evidence and poor work environment', and major facilitating factors were 'improving knowledge and skills related to evidence-based practice' and 'communicating and sharing evidence'. Conclusion: The study findings provide useful information for understanding nurses' experience of using external and internal evidence along with their meaning. A multidimensional approach is needed to overcome barriers to and implement evidence-based practice.

• Journal of the Korean Society for Library and Information Science
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• v.43 no.4
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• pp.5-27
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• 2009

The Library and Information Policy Committee announced a national comprehensive plan for all libraries in August, 2008. The plan includes the re-establishment of a subject-based national library system as one of the major policy challenges. Based on the preceding study (an analysis on systematization and the gaps in subject-based national libraries in major countries), this paper proposed three models: a centralized model focused on the NLK, a horizontally-linked model, and a long-term strategic model for subject-based national library systematization in Korea.

• Food Science and Biotechnology
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• v.15 no.1
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• pp.128-132
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• 2006

This study was undertaken to investigate the effect of peptic and chymotryptic hydrolyses of 7S globulin, the major allergen of soybean protein, on its allergenicity, as measured by enzyme linked immunosorbent assay (ELISA), and to identify the allergenic hydrolyzed fragments of 7S globulin using SDS-PAGE. When 7S globulin was hydrolyzed by pepsin, the allergenicity was reduced by over 50%. However, the allergenicity of 7S globulin reduced by peptic hydrolysis was recovered in the sera from 5 out of 10 patients following sequential chymotryptic hydrolysis. Two fragments, with molecular weights 20-25 and 13-16 kDa, among the hydrolysate of 7S globulin by sequential pepsin and chymotrypsin showed reactivity with sera from 10 soybean-allergenic patients. As a result of the theoretical hydrolyses of ${\beta}$-conglycinin, which is a major protein of 7S globulin, it is suggested that the 20-25 kDa fragments were the fragments of the ${\alpha}$-subunit of ${\beta}$'-conglycinin and that the 10-16 kDa fragments were from the ${\alpha}$'-subunit.

• Journal of the Economic Geographical Society of Korea
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• v.6 no.2
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• pp.293-306
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• 2003

This paper aims to clarify the change in industrial structure and industry itself, and makes suggestions for the industrial development direction at transitional periods in the Chungcheongbuk-do(province) region. Because profits of regional gross production in Chungcheongbuk-do region flow out of the region, basic industries must be brought up. For this phenomenon, main manufacturing must be developed for the industrial power of the next generation of high added values that combined with digital industry; the petrochemistry, semiconductor industry as major type of industry, and automobile industry as minor type of industry. Also for supporting industry, education service, health and welfare, research and development services that are knowledge-based service industries in Chungcheongbuk-do region, must be formated the network among corporations and constructed regional innovation system linked with educational institutions, precision chemistry industry and biology technology as major type of industry, and precision machinery and tools industry as minor type of industry.

• Journal of Periodontal and Implant Science
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• v.46 no.1
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• pp.2-9
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• 2016

Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis, and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.10a
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• pp.415-445
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• 1998

For effective prevention of the spreading and outbreak of crop insects and disease pests, an intensive Pest surveillance system was established to predict their density changes, and distribution. After their initial establishment by either immigration or overwintering, it is necessary to anticipate how they spread out geographically and predict where/when outbreaks are possible. The two major tools, boundary layer atmospheric model (Blayer) and the geographic information system(GIS), have been being developed to facilitate the prediction of pest occurrence in recent days. We are also developing the PeMos (Pest Monitoring System) that is able to manage the pest surveillance data collected from 152 pest monitoring stations in Korea. These three system related to the pest surveillance should be integrated into an internet based comprehensive database management system to facilitate information resources systematically organized and closely linked. Considering various data types and large data size in each system, a new special information management system is suggested. The integrated system should express complex types of information, such as text, multimedia, and other scientific data under the Internet environment. This paper discussed the major three systems, GIS, Blayer, and PeMos, relevant to the crop pest surveillance, then how they can be integrated in a comprehensive system under the Internet environment.

• Journal of Environmental Science International
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• v.30 no.10
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• pp.811-820
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• 2021

The population variation of Spanish mackerel (Scomberomorus niphonius) according to its major prey abundance was analyzed using monthly catches of coastal set net fisheries in the southern waters off Gyeongsangnam-do and eastern waters off Gyeongsangbuk-do of Korea from 2006 to 2019. The abundance of Spanish mackerel and its prey species fluctuated almost simultaneously with time lags of +2 to -2 months between the set net fisheries in the southern and eastern waters. The generalized additive model revealed that the abundance of Spanish mackerel was influenced by its prey species such as hairtail and anchovy in southern waters, and common mackerel and horse mackerel in eastern waters. The model deviance explained 49% and 42% of Spanish mackerel abundance in southern and eastern waters respectively. These results suggest that the abundance of Spanish mackerel is affected by seasonal migratory prey fish species in the coastal areas and can be linked to their northerly migration.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.193-199
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• 2018

As animal welfare issue becomes important, the European Union bans conventional cages for laying hens from 2012. So the alternative housing systems like floor pens, aviaries or free range systems have been suggested. From 2011 to 2014, we monitored 20 welfare-oriented laying hen farms in South Korea to figure out serological status of major viral diseases. During this period, total 3,219 blood samples were collected from the randomly selected chickens to test and evaluate the hemagglutination inhibition titers for low pathogenic avian influenza, Newcastle disease and egg drop syndrome '76. A total of 2,926 blood samples were tested through enzyme linked immunosorbent assay (ELISA) to assess the serological status of infectious bronchitis (IB). The distribution of ELISA titers for IB was various from almost 0 to 20,000 through the all weeks of age. Also, the antibody coefficient of variation for most of the diseases in this study was higher than those of typical cage layers. As this study was the first surveillance for major avian viral diseases of the animal welfare-oriented farms in South Korea, the results obtained from this study will help to determine what information and resources are needed to maintain better biosecurity and to improve the health and welfare of laying hen flocks.