• KSBB Journal
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• v.16 no.2
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• pp.99-106
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• 2001

Proteomics research includes identification and quantitation of single protein and/or protein complex, profiling of protein expression changes in response to biological perturbations, characterization of protein functions and interactions, and elucidation the linkage between proteins and diseases. In this review paper, recent developments in the basic technologies involved in the proteomics research such as 2-dimensional PAGE and mass spectrometry are discussed. Also, the application areas of proteomics technology such as protein expression mapping and cell map proteomics are introduced with the focus on new drug development.

• Knowledge Management Research
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• v.21 no.2
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• pp.41-58
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• 2020

A plan to promote public technology-based start-ups as a way to enhance the use of public technology has been emphasized. However, the problem of creating commercial value has persisted due to the lack of reproducibility and readiness of public technology. Although gap exists in the linking process between the public and private sector, it is difficult to find objective empirical evidence as most of them are only qualitative research. Our empirical works examine the impact of subsequent support of the public research institute on the commercialization of public technologies. Then, we analyze how commercialization of public technologies affects the growth of technology-based startups using 2-stage least square (2SLS) model. We find that the linkage with the technology subject contribute to the successful commercialization of public technology, and further to the survival and growth of technology-based start-ups. Our finding suggests that knowledge management system, to facilitate interaction between technology developer and consumers, is needed for the success of public technology-based start-ups.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.235-240
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• 2014

Cucumber mosaic virus (CMV) is one of the most important viral diseases in pepper (Capsicum annuum L.), and several genes for resistance were reported in Capsicum spp. In Korea, a single dominant gene that is resistant to $CMV_{Fny}$ and $CMV_{P0}$ has been used for breeding. Recently, a new strain ($CMV_{P1}$) was reported that could infect cultivars resistant to both $CMV_{Fny}$ and $CMV_{P0}$. Therefore, breeding of more robust CMV-resistant cultivars is required. In this study, we surveyed the inheritance of $CMV_{P1}$ resistance and analyzed the location of the resistance loci. After $CMV_{P1}$ inoculation of various germplasms and breeding lines, one accession (ICPN18-8) showed no visual symptoms at 15 dpi (days post inoculation) but was susceptible after 45 dpi, and one resistant line (I7339) showed resistance until at 45 dpi. The latter line was used for tests of resistance inheritance. A total of 189 $F_2$ plants were examined, with 42 individuals showing resistance at 15 dpi and a phenotype segregation ratio close to 1:3 (resistant:susceptible plants). In a lateral ELISA test at 45 dpi, 11 plants showed resistance, and the segregation ratio was changed to 1:15. These results indicate that resistance in C. annuum 'I7339' is controlled by two different recessive genes; we named these resistance genes 'cmr3E' and 'cmr3L,' respectively. To locate these two resistant loci in the pepper linkage map, various RAPD, SSR, and STS markers were screened; only nine markers were grouped into one linkage group (LG). Only one RAPD primer (OPAT16) was distantly linked with cmr3E (22.3 cM) and cmr3L (20.7 cM). To develop more accurate markers for marker-assisted breeding, enriching for molecular markers spanning two loci will be required.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.1
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• pp.254-260
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• 2013

This paper deals with electromagnetic characteristics of IPMSM (Interior Permanent Magnet Synchronous motor) caused by field weakening current control. In order to extend operation speed, field weakening current control is generally used in IPMSM operation. During field weakening, distorted linkage fluxes are resulted by saturation of core material. Therefore, distorted input voltage waveform is required for sinusoidal current input. As the current vector angle increases for field weakening, distortion of linkage flux and back-emf becomes significant. This situation is analyzed by 2-dimensional finite element analysis and verified by experiment. With the results, it is concluded that motor parameters, such as linkage flux by permanent magnet, phase resistance, d-q axis inductance, are insufficient for estimating required voltage for given speed especially in field weakening and additional considerations for increased harmonics of voltage are required.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.550-558
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• 2015

To analyze the linkage relationships among molecular markers recently reported to be linked to onion (Allium cepa L.) Ms, a restorer-of-fertility locus, in onion (Allium cepa L.), three single nucleotide polymorphism markers were converted into cleaved amplified polymorphic sequence (CAPS) markers based on onion transcriptome sequences and the rice genome database. Analysis of the recombinants selected from 4,273 segregating plants using CAPS and other linked markers demonstrated the jnurf13 and jnurf610 markers to perfectly co-segregate with the Ms locus. In contrast to jnurf13, the jnurf610 marker was not in perfect linkage disequilibrium with the Ms locus in diverse breeding lines. Thus, the jnurf13 marker and the marker for identification of cytoplasm types were utilized to enhance the efficiency of onion breeding through four applications. First, 89 maintainer lines containing the normal cytoplasm and homozygous recessive Ms genotypes were successfully identified from 100 breeding lines. Second, these two molecular markers were used to analyze the main sources of male-fertile contaminants frequently found in the male-sterile parental lines during F1 hybrid seed production. The majority of the contaminants contained heterozygous Ms genotypes, indicating that pollen grains harboring the dominant Ms genotype may have been introduced during propagation of the maintainer lines. Therefore, the genetic purity of the two maintainer lines was analyzed in the third application, and the results showed that both maintainer lines contained 13-21% off-types. Finally, the two markers were used to increase the seed yield potentials of two open-pollinated varieties containing sterile cytoplasms by removing the plants harboring homozygous recessive and heterozygous Ms genotypes.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.559-565
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• 2015

The rind phenotype of watermelon fruits is an important agronomic characteristic in the watermelon market. Inheritance and linkage analyses were performed for three rind-related traits that together determine the rind phenotype: foreground stripe pattern, rind background color, and depth of rind color. The inheritance of the foreground stripe pattern was analyzed using three different $F_2$ populations, showing that the striped pattern is dominant over the non-striped pattern. The inheritance analysis of the rind background color was performed using $F_2$ populations of the '10909' and '109905', and the depth of rind color was analyzed using $F_2$ populations of the '90509' and '109905'. Yellow color was found to be dominant over green color, and a deep color was dominant over the standard color. Linkage analysis of the three traits was conducted using three $F_2$ populations in which two traits were segregating. Each pair of traits was inherited independently, which demonstrated that the three traits are not linked. Therefore, we propose a three-locus model for the determination of rind phenotype, providing novel insight that rind phenotype is determined by the combination of three genetically independent loci.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.20 no.5
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• pp.79-88
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• 2020

This paper propose the method to drive a solar panel cleaning robot efficiently on an inclined panel using vacuum pad pressure. In this method, the rubber pads using the vacuum pressure are used to attach robot body to the panel surface. By applying the linkage mechanism to the vacuum pads, it was possible to reduce robot weight and power consumption and to prevent slipping of the robot. In addition, the use of solenoid valves, proximity sensors, and encoders to detect movement of the robot body and the control of the pad pressure dedicate to the driving of the robot on an inclined panel. In order to move the robot forward, the operation sequence of multiple solenoid valves was completed, and the six vacuum pads mounted to both legs were accurately controlled to form vacuum and atmospheric pressure in right order so that the robot could move forward without slipping. At last, it was confirmed through experiments that straight-forward moving and rotational movement could be performed up to 36 degrees of inclination angle of solar panel.

• Proceeding of EDISON Challenge
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• 2016.03a
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• pp.455-462
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• 2016

Recently, followed by rapid growth of robotics field, multi-linkage mechanism which can even pass by rough road is getting lots of attention. In this paper, I focused on Jansen mechanism. It's a kinematics object which is named after Dutch artist Theo jansen. Jansen mechanism embraces structure and mechanism which creates locomotion with the combination of the power and simple structure. Theo jansen suggests a 'Holy number'. It's an ideal ratio of leg components length. However, if there's desired gait locomotion, you have to adjust the ratio and the length. But even slight change of the length could cause a big change at the end-point. To solve this problem, I suggest a reverse engineering method to get a ratio of each links by nonlinear optimization with pre-set desired trajectory. First, we converted a movement of the joint of Jansen mechanism to vectors by kinematics analysis of multi-linkage structure. And we showed the trajectory at the end-point. After that, we set desired trajectory which we found most ideal. Then we got the length of the leg components which draws a trajectory as same as trajectory we set, using Multi-objective genetic algorithm toolbox in MATLAB. Result is verified by Edison designer and mSketch. And we analyzed if it could pass through the obstruction which is set dynamically.

• Journal of The Korean Society of Agricultural Engineers
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• v.60 no.5
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• pp.29-40
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• 2018

Even if a small amount of arsenic (As) is entering to small agricultural reservoir from upper streams, small agricultural reservoir becomes sensitive to changes in arsenic concentration depending on the water level in case of accumulation continuously because of its scale. If we want to manage arsenic concentration in small agricultural reservoir, it is very important to understand arsenic changes in agricultural reservoir. In spite of the fact that modeling is the most accurate method for analyzing arsenic concentration changes in small agricultural reservoirs, but, it is difficult to monitor arsenic change everyday. So, if data is prepared for modeling arsenic changes, water quality modeling is more effective than monitoring. Therefore, in this study, arsenic concentration changes was simulated and arsenic concentration change mechanism in small reservoir was analyzed using hydrological and water quality monitoring data and by conducting EFDC (Environment Fluid Dynamics Code)-WASP (Water Quality Analysis Simulation Program) linkage. EFDC-WASP coupling technique was very useful for modeling arsenic changes because EFDC can consider hydrodynamic and WASP can perform arsenic concentration simulation, separately. As a results of this study, during dry season, As concentration was maintained relatively high arsenic concentrations. Therefore, water level control will be needed for managing As concentration of reservoir.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.2
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• pp.1-10
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• 2021

Agricultural reservoirs have a great influence on the water circulation in the watershed. It is necessary to evaluate the impact on water circulation by the agricultural reservoir. Therefore, in this study, we simulated the agricultural watershed through linkage of Hydrological Simulation Program Fortran (HSPF) and Module-based hydrologic Analysis for Agricultural watershed (MASA) and evaluated the contribution of the agricultural reservoir to water circulation by watershed water circulation index. As a result of simulating the Idong reservoir watershed through the HSPF-MASA linkage model, the model performance during the validation period was R2 0.74 upstream, 0.78 downstream, and 0.76 reservoir water level, respectively. To evaluate the contribution of agricultural reservoirs, three scenarios (baseline, present state, and present state without reservoir) were simulated, and the water balance differences for each scenario were analyzed. In the evaluation through the agricultural water circulation rate in the watershed, it was found that the water circulation rate increased by 1.1%, and the direct flow rate decreased by 13.6 mm due to the agricultural reservoir. In the evaluation through the Budyko curve, the evaporation index increased by 0.01. Agricultural reservoirs reduce direct runoff and increase evapotranspiration, which has a positive effect on the water circulation.