• Journal of Plant Biotechnology
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• 제44권4호
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• pp.349-355
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• 2017

The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.

• Asian-Australasian Journal of Animal Sciences
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• 제23권7호
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• pp.833-847
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• 2010

In the past decade, there have been many advances in whole-genome sequencing in domestic animals, as well as the development of "next-generation" sequencing technologies and high-throughput genotyping platforms. Consequently, these advances have led to the creation of the high-density SNP array as a state-of-the-art tool for genetics and genomics analyses of domestic animals. The emergence and utilization of SNP arrays will have significant impacts not only on the scale, speed, and expense of SNP genotyping, but also on theoretical and applied studies of quantitative genetics, population genetics and molecular evolution. The most promising applications in agriculture could be genome-wide association studies (GWAS) and genomic selection for the improvement of economically important traits. However, some challenges still face these applications, such as incorporating linkage disequilibrium (LD) information from HapMap projects, data storage, and especially appropriate statistical analyses on the high-dimensional, structured genomics data. More efforts are still needed to make better use of the high-density SNP arrays in both academic studies and industrial applications.

• 융합보안논문지
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• 제19권4호
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• pp.173-180
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• 2019

Cyber Physical System으로 상징되는 제4차 산업혁명(Industry 4.0)이 전 세계적으로 확산되어 감에 따라 향후 구현될 다양한 사이버 시스템들의 기술적 안정·신뢰성을 확보하기 위해서는 융합보안 인프라 구축이 필수적이다. 본 연구에서는 4차 산업혁명의 확산과 관련하여 생활안전의 개념을 포함하는 광의의 융합보안산업 및 기술의 현상을 파악하고, 관련된 지식간의 연계 가능성을 분석하여 보안의 융합화에 필요한 산학협력을 촉진할 수 있는 인력양성, 기술개발, 정책개선 등에 관한 종합적인 발전 정책을 제안하고자 한다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.117-117
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• 2017

The onion (Allium cepa L.) is the most widely cultivated species of the genus Allium, especially it has been valued because of the pungent flavor and aroma. Allium species including onion has very large genome sizes ranging from approximately 10 to 20 Gbp, which have complicated genomic studies and precluded genome sequencing until recently. A population of 186 F2 individuals derived from a cross of 'Umjinara' ${\times}$ 'Sinsunhwang' and the two parental lines were used for this study. For the development of framework map, various types of markers including SSRs, RAPD, SNPs, and CAPS makers have been used for polymorphism test. Especially, a lot of SNP and CAPS loci were developed from the onion transcriptome sequence by RNASEQ of two parental lines. The GBS libraries have been constructed based on a modified protocol from Poland Lab using a two-enzyme system. We have been developing markers showing polymorphism between two parental lines, and genotyping for all F2 individuals were finished for a number of polymorphic markers. For the construction of GBS libraries, a set of 192 barcoded adapters were generated from complementary oligonucleotides with XhoI overhang sequence and unique barcodes of length 4-8 bp and they have been tested using two parental linesto determine the optimum conditions for GBS analysis.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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• pp.14-17
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• 2001

Positional cloning (map-based cloning) of mutations or genetic variations has been served as an invaluable tool to understand in-vivo functions of genes and to identify molecular components underlying phenotypes of interest. Mice homozygous for the cerebellar deficient folia (cdf) mutation are ataxic, with cerebellar hypoplasia and abnormal lobulation of the cerebellum. In the cdf mutant cerebellum approximately 40% of Purkinje cells are ectopically located within the white matter and the inner granule cell layer (IGL). To identify the cdf gene, a high-resolution genetic map for the cdf-gene-encompassing region was constructed using 1997 F2 mice generated from C3H/HeSnJ-cdf/cdf and CAST/Ei intercross. The cdf gene showed complete linkage disequilibrium with three tightly linked markers D6Mit208, D6Mit359, and D6Mit225. A contig using YAC, BAC, and P1 clones was constructed for the cdf critical region to identify the gene. A deletion in the cdf critical region on chromosome 6 that removes approximately 150kb of DNA was identified. A gene associated with this deletion was identified using cDNA selection. cdf mutant mice with the transgenic copy of the identified gene restored the brain abnormalities of the mutant mice. The positional cloning of cdf gene provides a good example showing the identification of a gene could lead to finding a new component of important molecular pathways.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.163-170
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• 2008

The detection, characterization and use of quantitative traits loci, QTL, have significant potential to improve the efficiency of selective breeding of species. Therefore, a population with 59 advanced backcross lines($BC_2F_5$), derived from a cross between IR64 and Tarome molaei, were studied in Tonekabon Rice Research Station of Iran in order to map QTLs for panicle length, number of grain per panicle, and panicle grain sterility in rice. The parental screening wtih 235 SSR markers in agarose and polyacrylamide gels revealed 114 markers with clear polymorphic bands. To search for QTLs associated with panicle length, number of grain per panicle, and panicle grain sterility, we constructed a genetic linkage map using 114 microsatellite markers. Positive and negative transgressive segregations were observed in $BC_2F_5$ lines for all traits. Using multiple interval mapping(MIM), a total of 20 putative QTLs were detected, of which eight were for panicle length, three for number of grains, and nine for panicle grain sterility. The maximum number of QTLs were mapped on chromosomes 1 and 2 with eight QTLs. These QTL markers could possible be utilized for marker-assisted selection.

• International Journal of Internet, Broadcasting and Communication
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• 제14권4호
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• pp.181-188
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• 2022

The purpose of this study is to identify selection attributes, functional benefits, psychological benefits, and values according to crossover shopping patterns (showrooming and webrooming). To achieve objectives of this study, a survey was designed based on the means-end chain theory, using the in-depth laddering technique and APT laddering technique which understanding the linkage of A(attributes)-FB(functional benefits)-PB(psychological benefit)-V(value). These two laddering techniques were used to construct a hierarchical value map (HVM) by linking selection attributes, functional benefits, psychological benefits, and value levels. The selection attribute items that showrooming shoppers consider important are 'price conformity', 'product information', 'product variety', and 'delivery service'. Functional benefit items were 'free purchase', 'economic benefit', 'communication', 'safety', and 'accurate Information', and psychological benefit items were 'convenience', 'relaxation', 'pleasure', 'rational consumption', and 'stability'. Finally, the value items were 'self-satisfaction', 'abundant life', 'achievement', 'happiness', and 'reasonable life'. Next, the selection attribute items that webrooming shoppers consider important are 'price conformity', 'product information', 'product variety', 'AS', 'shopping atmosphere', and 'seller service'. Functional benefit items were 'free purchase', 'economic profit', 'expression opinion', 'safety', and 'accurate information', and psychological benefit items were 'convenience', 'relaxation', 'rational consumption', and 'stability'. Finally, the value items were 'self-satisfaction', 'abundant life', 'happiness', and 'reasonable life'.

• 원예과학기술지
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• 제28권6호
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• pp.1014-1024
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• 2010

고추 탄저병은 국내외에서 고추 생산에 있어 많은 피해를 주는 병중의 하나로서, $Colletotrichum$ spp.에 의해 발생하며 현재 가장 많은 빈도를 나타내는 종은 $C.$ $acutatum$으로 보고되고 있다. 살균제에 대한 내성이 있는 것으로 보고되어 저항성 품종의 육성이 요구되어지고 있다. 본 연구의 목적은 $Capsicum$ $baccatum$ 종내 교잡을 통해 고추 탄저병 저항성 육종을 위한 MAS(marker assisted selection) 체계를 개발하는 기초연구로서 저항성과 관련한 QTL분석을 수행하였다. 저항성 검정결과 고추 탄저병저항성은 85%의 높은 광의의 유전력을 보였다. 양적형질 유전자좌 분석 결과 신뢰수준 이상의 LOD 수준을 보인 2개의 QTL($An8.1$, $An9.1$)이 탐색되었고, $R^2$가 2.04%와 14.36%이었다. 유전적인 효과는 $An8.1$은 상가적 효과가 0.46으로, $An9.1$은 상가적 효과가 -4.52로 나타났다. $An8.1$과 $An9.1$은 상처접종에 의해 나타나는 저항성 반응과 관련이 있는 것으로 생각되었다. 포장검정(08NR)과 관련한 QTL들은 신뢰수준이상의 LOD수준을 보이는 QTL은 탐색할 수 없었으나, $R^2$가 19.9%인 $An3.1$와 30.8%인 $An3.1$을 포함한 5개 QTL의 전체 $R^2$가 60.73%로 나타나 고추 탄저병저항성 유전과 관련한 인자가 다수 존재하는 것을 확인하였다. 따라서 이들 QTL은 더욱 정밀한 집단과 종간교잡 후대에서 적용성을 검정하여 고추 탄저병저항성 품종육성을 위한 MAS체계를 확립할 것이다.

• 한국지역지리학회지
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• 제13권5호
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• pp.563-575
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• 2007

본 연구는 개방환경에서 지형정보 웹지도화를 통한 활용가능성에 대한 검토 분석이다. 웹지형도는 기존 지형도의 기능을 수행하면서 동적인 지형정보 활용을 위한 기능과 분석, 시각화에 초점을 두고자 하였다. 웹지형정보도의 주요 기능 구현으로는 공간검색, 확대 축소, 이동, 토지이용 정보, 사용자정의 3차원 표현, 지형단면 및 고도분석, 거리분석, 지역간 경로안내, 지역검색, 필드정보, 지역 영상정보 등이다. 이러한 기능을 수행하기 위해 SVG, MYSQL, PHP, XML을 사용하였다. 공간정보는 SVG를 이용하여 표현되도록 하였다. SVG는 소스가 개방되어 누구나 쉽게 사용할 수 있을 뿐만 아니라, 웹언어를 통한 데이터베이스 연동, 지도학적 표현이 효과적이다. 본 연구에서 중점을 두어 개발한 기능은 사용자 정의 3차원지도, 지형단면도, 최단경로 분석이다. 3차원지도는 수치고도모델을 제작하여 고도에 따른 픽셀에 불투명도 간을 부여하여 태양빛에 따라 입체영상이 표현되도록 하였다. 지형단면분석은 사용자가 범위를 지정하면 수치고도모델의 고도간을 데이터베이스에서 가져와 지형단면도과 통계정보가 나타나도록 하였다. 지역간 최단경로는 다익스트라 알고리즘을 적용하여 개발하였다. 앞으로 WebGIS는 정보전달 보다는 사용자 제공 지리정보(WebGIS 2.0) 확산에 더 기여할 것으로 예상된다. 이를 위하여 보다 많은 WebGIS 에 대한 연구개발이 요구된다.

• Journal of Microbiology and Biotechnology
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• 제11권6호
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• pp.1115-1119
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• 2001

Eight Escherichia coli cells with aerobic growth deflects were isolated by the insertion of ${\lambda}placMu53$, a hybrid bacteriophage of ${\lambda}$ and Mu, which created transcriptional fusion to lacZY. Two of these mutants, CLIO and CLl2, were irradiated with UV to obtain specialized transducing phages. The phages that took out the neighboring chromosomal DNA of the related gene responsible for deflective aerobic growth were identified. The in vivo cloned chromosomal sequence revealed that the mutated gene of CLIO was located at min 34.5 on the Escherichia coli linkage map and 1,599,515 on the physical map. The physical map indicated that there were 7 cistrons in the operon. We named this operon oxg10. The promoter sequence of oxg10 exhibited a possible binding site far SoxS, a transcriptional regulator that activates the transcription of various SoxRS regulon genes. Transferring the oxg10:: ${\lambda}placMu53$ mutation into the wild-type strain, RZ4500, resulted in the inhibition of normal aerobic growth, while the salute mutation in strain MO inhibited aerobic cell growth completely. The full operon sequences of oxg10 were cloned from the Excherichia coli genomic library. The mutated gene of CLl2 was identified to be a sucA gene encoding the ${\alpha}$-ketoglutarate dehydrogenase El component in the TCA cycle.