• Communications for Statistical Applications and Methods
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• 제31권4호
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• pp.409-425
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• 2024

Logistic regression models are commonly used to explain binary health outcome variable using independent variables such as patient characteristics in medical science and public health research. Although there is no distributional assumption required for independent variables in logistic regression, variables with severely right-skewed distribution such as lab values are often log-transformed to achieve symmetry or approximate normality. However, lab values often have zeros due to limit of detection which makes it impossible to apply log-transformation. Therefore, preprocessing to handle zeros in the observation before log-transformation is necessary. In this study, five methods that remove zeros (shift by 1, shift by half of the smallest nonzero, shift by square root of the smallest nonzero, replace zeros with half of the smallest nonzero, replace zeros with the square root of the smallest nonzero) are investigated in logistic regression setting. To evaluate performances of these methods, we performed a simulation study based on randomly generated data from log-normal distribution and logistic regression model. Shift by 1 method has the worst performance, and overall shift by half of the smallest nonzero method, replace zeros with half of the smallest nonzero method, and replace zeros with the square root of the smallest nonzero method showed comparable and stable performances.

• 분석과학
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• 제37권4호
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• pp.230-238
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• 2024

This study evaluates a method for quantifying selenium (Se) concentration in soil using inductively coupled plasma mass spectrometry (ICP-MS), with oxygen as a reaction gas. This approach addresses the challenge of detecting low levels of Se in complex soil matrices and aims to effectively minimize interference problems typically associated with argon plasma in traditional ICP-MS analyses. The analytical method utilizes conditions optimized for minimizing spectral interference and were validated by linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The method demonstrated good linearity, high accuracy (90-97 %), and remarkable sensitivity, achieving detection and quantification limits of 0.15 ㎍/kg and 0.44 ㎍/kg, respectively. Developed analysis method for Se in soil was applied to field samples in the different regions of South Korea and Se concentration ranged from 0.11 to 0.52 mg/kg. Correlation analysis between Se concentration and soil properties showed that Se concentration was significantly correlated with cation exchange capacity (CEC) and available phosphorus among other soil properties.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.322-328
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• 2005

Acylhomoserine lactones (AHLs) are known to be the triggering molecules in the quorum sensing mechanism of many gram-negative bacteria. In order to detect AHL inhibitors that are potential biofilm inhibitors, a convenient and sensitive bioassay was developed based on the $\beta$-galactosidase activity ($\beta$-GAL) of a recombinant Agrobacterium tumefaciens strain. A series of commercially available AHLs were tested for inducing $\beta$-GAL at varying concentrations in agar-plate and liquid cultures of the reporter strain. All AHLs tested exhibited a concentration­dependent induction, and octanoyl homoserine lactone (OHL) showed the highest sensitivity with a detection limit of 0.1 nM in the liquid culture assay. When fimbrolide, a known quorum sensing inhibitor, was added, induction of $\beta$-GAL by OHL was repressed. The repression at a constant OHL concentration was dependent on the fimbrolide concentration with the detection limit below 1 ppm, indicating that this assay is a sensitive method for screening AHL inhibitors.

• 분석과학
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• 제7권3호
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• pp.253-260
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• 1994

Low power-low flow 유도결합 플라즈마 원자방출 분광법(ICP-AES)에서 분석적 특성에 대해 연구하였다. Low power ICP에서는 moderate power ICP보다 알짜세기는 감소하지만 바탕세기도 감소하여 알짜세기에 대한 바탕세기의 비는 오히려 증가하였다. Low power ICP에서도 작동조건에 따라 moderate power와 비슷한 검출한계를 얻을 수 있으며, 검량곡선도 $10^4{\sim}10^5$ 정도의 직선성을 가질 수 있었다. 알칼리 금속에 의한 이온화 방해영향은 시료운반기체의 사용량을 증가시킬수록 증가하지만, RF power의 변화에 대해서는 큰 차이가 없었다.

• Applied Microscopy
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• 제41권2호
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• pp.81-88
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• 2011

최근 나노 영역에서의 구조분석과 조성분석의 중요성이 증대되고 있으나, 기존의 분석장비들은 한계에 부딪히고 있다. 최근 개발된 APT는 nm 이하의 공간분해능과 수십 ppm수준의 detection limit으로 원소의 3차원분포와 조성정보를 제공해 주는 분석장비로서, 이러한 기존 분석의 한계를 극복할 수 있는 새로운 분석장비이다. 그러나 국내에는 아직 잘 알려지지 않아 활용이 미비한 실정이다. 따라서, 본 논문에서는 APT에 대한 이해를 돕기 위해 APT분석의 원리와 시편준비에 대해 소개하였다.

• 분석과학
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• 제8권3호
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• pp.237-248
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• 1995

미국 환경청이 지정한 129종의 priority pollutants 중 16종의 유기살충제를 포함한 토양시료를 제조하여 표준분석방법인 기체크로마토그래프-전자포획법과 기체크로마토그래프/질량분석기-SIM (selected ion monitoring)법을 이용하여 동시분석의 효율성을 실험하였다. 토양시료의 추출 및 농축방법으로는 EPA의 SW-846 방법을 조금 수정한 초음파추출법(sonication extraction)과 Soxhlet 추출법(Soxhlet extraction)을 비교 연구하였다. 각 화합물의 회수율, 상대표준편차 및 method detection limit를 측정하여 그 결과들을 보고하였다.

• 대한화학회지
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• 제28권1호
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• pp.41-46
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• 1984

미량 금속원소를 농축하기 위하여 수용액중의 구리, 코발트, 니켈, 아연 및 카드뮴을 8-히드록시 퀴놀린(옥신)으로 착물을 만들고 스테아르산으로 추출하였다. 추출한 것을 녹여서 본틀에 부어 시편을 만들고 X-선 형광 분석법으로 정량하였다. 이때 침전 생성 및 추출조건, 재현성, 감도 검출한계 등에 관하여 조사하였다. 시편 제작과정의 상대 표준편차는 1.0~5.7%이고, 검출한계는 5~$50{\mu}g$/100ml이었다. 이 농축법으로 감도를 높일 수 있으며 시편 제작이 편리한 잇점이 있다.

• 생약학회지
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• 제35권4호통권139호
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• pp.276-282
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• 2004

This study has been conducted to investigate the amount of residual sulfur dioxide for herbal medicines of 30 species which are purchased in 13 different regions (Bonghwa, Busan, Chunju, Jecheun, Kwangiu, Keumsan, Seoul, Taeku, Cheungdu, Xian, Beijing, Tokyo and Osaka). The sulfur dioxide residues were determined in the collected 386 samples by the modified Monier-Williams method. The residues of sulfur dioxide in 386 samples ranged from ND (under detection limit) to 2808 ppm. The sulfur dioxide in Cassiae Semon, Cinnamomi Ramulus, Cervi Cornu, Hoelen, Crataegi Fructus, Artemisiae Argyi Herba, Scolopendrae Corpus, Schizandrae Fructus and Cyperi Rhizoma were detected under the detection limit regardless of the collected regions. The sulfur dioxide residues in herbal medicines collected in domestic regions were relatively lower than those in foreign regions. There are no standards for sulfur dioxide residual limits of herbal medicines in Korea. This results will provide the scientific basis for the standardization of sulfur dioxide residues in Korea Pharmacopoeia.

• 한국환경과학회지
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• 제10권1호
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• pp.13-19
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• 2001

An on-line digestion system based on the flow injection analysis technique was presented for the determination of total phosphorus. The system converts condensed and organic phosphoruses into orthophosphate that can be analyzed spectrophotometically. The reaction between orthophosphate and ammonium molybdate in a strong acid medium forms the yellow complex of phosphomolybdate which is next reduced to a molybdic blue complex by stannous chloride. The quantitation of orthophosphate is based on the absorbance of the molybdic blue. To determine total phosphorus, the digestion system was installed between the sample injector and the reaction coil with the added lines of digestion solution(potassium persulfate+sulfuric acid) and the $H_2O$ carrier. The system has shown that the digestion efficiencies were greater than 95% for the typical condensed and organic phosphoruses. When tripolyphosphate standards were used, the calibration data showed that the linear dynamic range extended to a concentration of 1.5ppm with the detection limit of 25ppb total phosphorus. The typical relative standard deviation was less than 2%. The proposed system was successfully applied to lake water, wastewater, and streamwater. The analytical variables such as digestion efficiency, analysis time, and reproducibility were evaluated and compared with the manual digestion method.

• 약학회지
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• 제42권2호
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• pp.149-152
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• 1998

A high performance liquid chromatographic method was developed for the determination of DA-6034 in biological fluids using internal standard. Plasma containing DA-6034 and inter nal standard was extracted by liquid-liquid extraction at an acidic pH. After evaporation of the organic layer, the drug and internal standard were reconstituted with mobile phase and injected into the column. They were separated by high performance liquid chromatography on inertsil ODS II column at 334 nm. The detection limit of DA-6034 in plasma was 0.02 ${\mu}$g/ml. In this method, the range of recovery and coefficients of variation were 96-110% and 0.40-3.78%, respectively. There was no interference from endogenous substances. Urine and bile were analysed using the deproteinization method and the detection limit of DA-6034 was 1${\mu}$g/l.