• The Plant Pathology Journal
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• 제38권5호
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• pp.503-512
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• 2022

Lilies (Lilium spp.) are one of the most important ornamental flower crops grown in Korea. Most viral diseases in lilies are transmitted by infected bulbs, which cause serious economic losses due to reduced yields. Various diagnostic techniques and high-throughput sequencing methods have been used to detect lily viruses. According to Oxford Nanopore Technologies (ONT), MinION is a compact and portable sequencing device. In this study, three plant viruses, lily mottle, lily symptomless, and plantago asiatica mosaic virus, were detected in lily samples using the ONT platform. As a result of genome assembly of reads obtained through ONT, 100% coverage and 90.3-93.4% identity were obtained. Thus, we show that the ONT platform is a promising tool for the diagnosis and characterization of viruses that infect crops.

• 농업과학연구
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• 제40권4호
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• pp.281-287
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• 2013

우리나라의 격리재배는 102속, 약 250여종의 식물에 대해 실시하고 있으며, 직접경검법, 배양법, 선택배지, 생리생화학, ELISA 및 PCR 검사방법을 사용한다. 2005-2012년, 우리나라에서 수행된 격리재배는 총 8,307건이며 이중 구근류가 5,165건(62.2%)로 가장 많았고, 묘목류가2,119건(25.0%), 종자 796건(9.6%), 삽수 150건(1.8%), 접수 70건(0.8%) 및 기타 7건(0.1%) 이었다. 불합격 사례는 총 413건으로 약 4.97%였고, 발견된 병의 종류는 총 47종으로 나타났다. 종류별로는 바이러스가 27종으로 가장 많은 수를 차지했으며, 곰팡이 16종, 바이로이드 1종, Chromalveolata 1종 및 기타 2종으로 나타났다. 가장 많은 검역건을 올린 병원체는 Arabis mosaic virus (77건), Tobacco rattle virus (70건), Lily symptomless virus (46건), Penicillium expansum (46건)이다.

• 원예과학기술지
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• 제33권6호
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• pp.891-899
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• 2015

본 연구는 자생나리에 있어서 기내 인편삽의 계대배양이 lily symptomless virus(LSV) 제거에 미치는 영향과 구근 재배 조건이 LSV 재감염에 미치는 영향을 구명하여 LSV 무병주 대량 생산을 위한 기초자료를 제공하기 위하여 실시되었다. 식물재료로는 날개하늘나리(L. dauricum), 말나리(L. distichum), 참나리(L. lancifolium), 중나리(L. maximowitzii)등 자생나리 4종을 사용하였다. LSV에 감염된 식물체의 인편에서 소인경을 얻은 다음 이들 소인경을 5회 이상 계대배양을 했을 때 LSV가 완전히 제거되었다. 특히 날개하늘나리와 말나리에서는 1회 기내 인편삽으로도 LSV를 완전히 제거할 수 있었다. 그러나 기내배양을 통해 생산된 LSV 무병주라도 구근의 재배기간이 길수록, 봄 정식구보다 가을 정식구에서, 소식구보다 밀식구에서, 해안지역보다는 내륙 지역에서 재감염률이 높았다. 그리고 재감염률은 중나리가 날개하늘나리와 참나리보다 높았다. LSV 감염주는 무병주에 비해 엽록소 함량이 낮았다. 따라서 자생나리 구근의 수회 계대배양을 통해 LSV를 제거할 수 있으며, 구근 생산과정에서 낮은 재식밀도, 봄 정식, 해안지역 정식 등 재배적 방제를 통해 LSV 재감염을 억제할 수 있을 것이다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.107.2-108
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• 2003

Viral disease symptoms were investigated in the field grown Longiflorum hybrid cultivars, and the damages caused by infection with Lily mottle virus (LMoV) and Cucumber mosaic virus (CMV) were assessed by comparing growth of plants produced from seeds of Longiflorum hybrid cultivar both infected by artificial inoculation and free from infection with theses viruses. Dominant symptom caused by spotaneous infection with LMoV and CMV in the field was mottle combined with chlorotic stripe on leaves. LMoV developed brownish necrotic lesion on floral leaves. The incidence of viral disease by mixed infection with LMoV, CMV or Lily symptomless virus (LSV) in the filed grown Longiflorum hybrid cultivar, cultivated for more than 6 years, was 80 to 84 percent. In comparison with virus-free plants, plants doubly infected with CMV and LMoV by artificial inoculation decreased stem length by 14 percent and fresh weight by 38 percent. In conclusion, flower quality and the stem length of Longiflorum hybrid cultivar were affected by LMoV and CMV infection.

• The Plant Pathology Journal
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• 제18권6호
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• pp.313-316
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• 2002

Systemic virus symptoms caused by a Potexvirus were observed on leaves of infected hosta (Hasta spp.) plants cultivated in Seoul, Korea. Symptoms on diseased hosta plants include mosaic, mottle, irregular blotchy patches, and chlorotic spots on or distortion of the leaves. No other viruses, such as Cucumber mosaic virus, Lily symptomless virus, or Potyvirus, were detected from the same plants by electron microscopy and by Western blot and RT-PCR analyses, indicating that they were singly infected by the potexvirus. The symptoms differed among cultivars and species of hosta, and affected the quality of plants for commercialization, as well as, plant growth and flowering of susceptible cultivars. Most of the cultivars and species investigated were susceptible to the virus, while some were not infected by the virus at all. Purified virus particles were of filamentous type with unaggregated forms 540 nm in length, which is a typical potexviral morphology. The virus consisted of a single-stranded RNA molecule of 6 kb long for genome and single component of coat protein (CP) about 27 kDa. The CP strongly reacted with the antiserum against Hosta vims X (HVX), suggesting that the virus is an isolate of HVX. This is the first report of the occurrence and identification of HVX from hosta plants in Korea.