The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Mosaic Disease of Hosta Plane Caused by Hosta virus X

  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Department of Horticultural Science, Division of Environmental and Life Sciences, Seoul Women’s University) ;
  • Park, Min-Hye (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women’s University, Department of Horticultural Science, Division of Environmental and Life Sciences, Seoul Women’s University) ;
  • Lee, Jong-Suk (Department of Horticultural Science, Division of Environmental and Life Sciences, Seoul Women’s University)
  • Published : 2002.12.01
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Abstract

Systemic virus symptoms caused by a Potexvirus were observed on leaves of infected hosta (Hasta spp.) plants cultivated in Seoul, Korea. Symptoms on diseased hosta plants include mosaic, mottle, irregular blotchy patches, and chlorotic spots on or distortion of the leaves. No other viruses, such as Cucumber mosaic virus, Lily symptomless virus, or Potyvirus, were detected from the same plants by electron microscopy and by Western blot and RT-PCR analyses, indicating that they were singly infected by the potexvirus. The symptoms differed among cultivars and species of hosta, and affected the quality of plants for commercialization, as well as, plant growth and flowering of susceptible cultivars. Most of the cultivars and species investigated were susceptible to the virus, while some were not infected by the virus at all. Purified virus particles were of filamentous type with unaggregated forms 540 nm in length, which is a typical potexviral morphology. The virus consisted of a single-stranded RNA molecule of 6 kb long for genome and single component of coat protein (CP) about 27 kDa. The CP strongly reacted with the antiserum against Hosta vims X (HVX), suggesting that the virus is an isolate of HVX. This is the first report of the occurrence and identification of HVX from hosta plants in Korea.

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References

  1. Brunt, A. A., Poster, G. D., Manelli, G. P. and Zavriev, S. K. 2000. Genus Potexvirus. In: Virus Taxonomy: Classification and Nomenctature of Viruses, Seventh Report of the Intemafional Committee on Taxonomy of Viruses. Van Regenmortel, M. H. V., Fauquet, C. M., BishoP, D. H. L., Carstens, E. B., Estes, M. K., Lemon, S. M., Maniloff, J., Mayo, M. A., McGeoch, D. J., Pnngle, C. R. and Wickner, R. B. (eds.). Academic Press, San Diego, PP. 975-981
  2. Currier, S. and Lockhart, B. E. L. 1996. Characterization of a potexvims infecting Hosta spp. Ptant Dis. 80:1040-1043 https://doi.org/10.1094/PD-80-1040
  3. Koenig, R. and Lesemann, D. E. 1978. Potexvirus group. Descripdons of Plant Viruses No. 200, CMI/AAB, Kew, Surrey, England
  4. Laemmli, U. K. and Favre, M. 1973. Maturation of the head of bactenophage T4. I. DNA packaging event J. Mot. Biot. 80:575-599 https://doi.org/10.1016/0022-2836(73)90198-8
  5. Ryu, K. H. and Choi, S. H. 2002. Molecular detecdon and analysis of Sweet potato feathery mottle vims from root and leaf tissues of cultivated sweet potato plants. Ptant PathoI. J. 18:12-17 https://doi.org/10.5423/PPJ.2002.18.1.012
  6. Ryu, K. H., Park, H. W. and Choi, J. K. 2002. Characterization and sequence analysis of a lily isolate of Cucumber mosaic virus from LiIium tsingtauense. Ptant PathoI. J. 18:85-92 https://doi.org/10.5423/PPJ.2002.18.2.085
  7. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Motecutar cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, NY
  8. Yoon, J. Y., Min, B. E., Choi, J. K. and Ryu, K. H. 2002. Genome structure and production of biologically active in vitro transcripts of cucurbits-infecting Zucchini green mottle mosaic virus. Phytopathotogy 92:156-163 https://doi.org/10.1094/PHYTO.2002.92.2.156

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