• 한국자원식물학회지
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• 제11권2호
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• pp.195-201
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• 1998

Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required $\beta$-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases.

• 농업과학연구
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• 제41권2호
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• pp.101-106
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• 2014

This study was conducted to identify lily species native to Korea from formosan lily (Lilium formosanum) belonging to Longiflorum section. Due to flowering time, flower color and orientation, long shelf life and resistant to diseases, the native lily species can be valuable genetic resources for interspecific hybrids. One of the chloroplast genes, matK, was used to clone and sequence to explore any base changes. The matK was successfully amplified into 1,539 bp (94% of the gene) and phylogenetic tree demonstrated 6 clades for those 11 lily species used in this study. There were one or two base substitutions among 10 lilies native to Korea, while formosan lily native to Taiwan exhibited 6 base substitutions in matK gene, rendering it genetically distant. A restriction enzyme NruI recognized one of the six base changes, and digested the matK gene of 10 native lily species only, but not in formosan lily. The confirmed cleavage characteristic of the target region in matK gene was designed into a CAPS (cleaved amplified polymorphic sequences) marker which will be available to estimate compatibility of interspecific hybridization and to trace the pedigree when those native lilies are crossed with the formosan lily.

• 한국자원식물학회지
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• 제18권3호
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• pp.450-455
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• 2005

아그로박테리움을 이용한 형질전환연구에 기초자료로 활용코자 기내배양중인 땅나리, 하늘나리, 대만나리의 잎절편으로부터 식물체를 유도하였다. 땅나리 잎절편으로부터 다수의 배양체를 얻기 위해서는 MS배지에 $0.2\;mg{\cdot}L^{-1}\;BA$와 $0.5\;mg{\cdot}L^{-1}\;NAA$를 첨가하여 암조건에서 배양하는 것이 효과적이었고, 하늘나리 잎 재분화에는 $0.2\;mg{\cdot}L^{-1}\;BA$와 $2.0\;mg{\cdot}L^{-1}\;NAA$ 혼용처리하는 것이 효과적이었으며, 대만나리의 잎 재분화에는 $1.0\;mg{\cdot}L^{-1}\;BA$와 $1.0\;mg{\cdot}L^{-1}\;NAA$ 혼용처리가 우수하였다.