• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.37-37
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which are cultivars and indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the non-contamination and survival rate after 2 weeks in culture. Non-contamination was shown to be 70 to 90% in formed small bulbs. This study will help to mitigate microbial contamination in Lilium species micropropagation for cryopreservation.

• Journal of Plant Biology
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• v.14 no.4
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• pp.14-18
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• 1971

Lilium Miquelianum Makino is a species which originated in Korea. The Karyotype of the species was examined in materials collected at Mts. Kaya, Kasan, Chejung, and Kaji. The results are as follows: 1) The somatic chromosome number was found to be 2n=24. 2) The karyotype is described as: K=2Am＋2Bkm＋2Csst＋2Dsst＋2Ests＋2Fst＋2Gst＋2Hst＋2Ist＋2Jsst＋2Kst＋2Lst m: metacentric, sm: submetacentric st: subtelocentric, s: secondary constriction 3) A single subcentric supernumerary B-chromosome was found in some bulbs from Mt. Kasan. 4) The shape of the supernumerary B-chromosome was similar to that of the E chromosome which had separated at its secondary constriction and lost its lower chromosome fragment. 5) From three to eight nucleio of varying sizes were found in the telophase or interphase nucleus of root tip cells. The maximum number of eight nucleoli corresponds to the number of chromosomes that have a secondary constriction.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.2
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• pp.66-74
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• 2008

In recent years, the view centered conservation for eco-system is getting important rather than development from human oriented perspective. As a result, the concept "Sustainable development", that is seeking harmony between conservation and development, appeared so that we researched distribution and status of vascular plants a site of project in the Bukkyungnam substation. The main findings from the study is as follows. The vascular plants a site of project in the Bukkyungnam substation area was listed 435 taxa; 93 families, 269 genera, 377 species, 1 subspecies, 51 varieties and 6 forms. Specific plant species by floral region were total 25 taxa; Acer palmatum (Planted species), Asperula lasiantha in Class III, Caryopteris incana, Cirsium schantarense in Class II, 21 taxa (Dryopteris erythrosora, Chloranthus japonicus, Salix glandulosa, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Clematis patens, Rubus hirsutus, Euphorbia pekinensis, Cayratia japonica, Grewia biloba var. parviflora, Vaccinium oldhami, Microstegium japonicum, Hosta capitata, Polygonatum falcatum, etc.) in Class I. Based on the list of Korean endemic plants, 10 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa (Planted species), Indigofera koreana, Forsythia koreana (Planted species), Paulownia coreana (Planted species), Asperula lasiantha, Weigela subsessilis, Adenophora triphylla var. hirsuta, Carex okamotoi, Lilium amabile and based on the list of the rare plants by the Forest ResearchInstitute, 2 taxa were recorded in the studied areas; Lilium callosum (Preservation priority order; No. 191), Aristolochia contorta (No. 151). The naturalized plants in this site were 13 families, 22 genera, 28 taxa and naturalization rate was 6.4% of all 435 taxa vascular plants.

• Korean Journal of Environment and Ecology
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• v.12 no.3
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• pp.191-211
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• 1998

The flora of the Kayasan National Park was surveyed from March to September, 1997. This area has very rich and diverse vascular plants; 613 taxa with 102 families, 323 genera, 522 species, 1 subspecies, 83 varieties, 5 forms and 2 hybrids. The rare and endangered plants species such as Lilium cernum Kom., Thymus quiquecostatus Celak., Cypripedium macranthum Sw., Tricyrtis dilatata Nakai, Abies koreana Wils., etc. were recorded. The plant species of 100 taxa such as Typha orientalis Presl, Corydalis maculata B. Oh et Y. Kim, Ajuga spectabilis Nakai, etc. were recorded for the first time in the area. The introduced plant species were found along the traffic road sides. The plant species of 14 taxa such as Lilium cernum Kom., Thymus quiquecostatus Celak., Abies koreana Wils., etc. which categorized as rare and endangered plants by the Office of Forestry were also recorded in the area(This species were mainly distributed in the habitats where easily threatened by human's activities).

• Korean Journal of Medicinal Crop Science
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• v.19 no.1
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• pp.9-15
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• 2011

This study was conducted to establish the optimal condition for conservation of genetic resources and micropropagation of Lilium cernum. Induction of bulbet of L. cernum was highly effective (9.2 bulb/explant) on 1/2 SH (Schenk and Hildebrandt) medium supplemented with 1.0 mg/L TDZ (Thidiazuron) and 0.1 mg/L NAA (Naphthaleneacetic acid). The treatment of 0.1 mg/L NAA increased root development (6.4 root/explant) under the in vitro condition. In addition, treatments of AC (Activated Charcoal) and ventilation were enhanced to develop number of shoots and to elongate length of leaf, bulb and root. Futhermore, the process of short-term soil acclimatization was promoted to strengthen the plantlets induced under the in vitro condition.

• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.236-245
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• 2021

This study aimed to characterize the morphological variations in the vegetative and floral traits of 73 wild Lilium amabile plants from six habitats in Korea. It was observed that L. amabile is distributed nationwide at any altitude from 300 m (Mt Mangdaeam) to 1550 m (Mt Halla). The majority of the natural habitats of L. amabile were found on mountain slopes, and some were found in rugged mountain regions. The down-facing flowers of this species not only had many blotches but also dense trichomes, and the flowering time was found to be from mid-June to mid-July. ANOVA revealed significant variations in vegetative and floral traits among the six habitats, indicating that the environment has substantial influences on the various growth parameters of L. amabile, such as plant height; number of leaves, bracts, papillae, and flowers; leaf angle; and lengths of the anther, longest blotch, and nectary of the petiole. In addition, the vegetative and floral traits were found closely correlated with each other under the direct impact of the environment. These findings will facilitate to find the appropriate environmental conditions for the conservation and development of L. amabile population as future lily-breeding materials.

• Journal of Environmental Science International
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• v.16 no.10
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• pp.1139-1146
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• 2007

The vascular plants in the Mt. Manduk was listed 560 taxa composed of 110 families, 345 genera, 488 species, 1 subspecies, 67 varieties and 4 forms. Based on the list of the rare plants by the Forest Research Institute, 4 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order; No. 159), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and Prunus yedoensis (No. 110). Based on the list of Korean endemic plants, 12 taxa were recorded; Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Populus tomentiglandulosa, Salix purpurea var. japonica, Pseudostellaria multiflora, Prunus yedoensis, Stewartia koreana, Forsythia koreana, Paulownia core ana, Weigela subsessilis and Aster koraiensis. Based on the list of approved for delivering overseas of plants, 7 taxa were recorded; Carex okamotoi, Lilium distichum, Aristolochia contorta, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha and Saussurea seoulensis. Specific plants by floral region were total 32 taxa; Prunus yedoensis in class V, Wistaria floribunda in class IV, 5 taxa (Girardinia cuspidata, Spiraea salicifolia, Acer palmatum, Stewartia koreana, Asperula lasiantha) in class III, 3 taxa (Potentilla dickinsii, Viola tokubuchiana var. takedana and Caryopteris incana) in class II and 22 taxa (Pinus koraiensis, Hosta capitata, Chloranthus japonicus, Salix glandulosa, Juglans mandshurica, etc.) in class I. The naturalized plants in the surveyed sites were 14 families, 36 genera, 44 species, 2 varieties, 46 taxa and naturalization rate was 8.2% of all 560 taxa of vascular plants. Wild plants disturbing ecosystem like Ambrosia artemisiifolia var. elatior have been increasing. Therefore, continuous control and conservation measures are needed on the ecosystem of Mt. Manduk.

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.151-152
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• 2010

• Korean Journal of Environment and Ecology
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• v.15 no.4
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• pp.293-318
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• 2002

As one of the botanical hot spots of the Korean peninsula, the vascular plant species in the areas of Taebaeksan, Hambaeksan, Geumdaebong(Peak) and Maebongsan were summarized as 694 taxa, 89 families, 302 genus, 579 species. 102 varieties and 13 forms. Based on the list of rare and endangered plants from the Forest Research Institute and the Ministry of Environment, 22 species were recorded in the studied areas: Thuja koraiensis(Cupressaceae), Arisaema heterophyllum(Araceae), Disporum ovale(Liliaceae), Tricyrtis dialata(Liliaceae), Lilium distichum(Liliaceae), Anemone koraiensis(Ranunculaceae), Rodgersia podophylla(Saxifragaceae), Rosa marretii(Rosaceae), Viola diamantica(Violaceae), Viola albida(Violaceae), Echinopanax horridum(Araliaceae), Acanthopanax chiisannensis(Araliaceae), Lysimachia coreana(Prinmulaceae), Syringa velutina var. kamibayashii, Halenia corniculata(Gentianaceae), Partrinia saniculaefolia(Valerianaceae), Adenophora grandiflora(Campanulaceae), Cacalia pseudo-taimingasa(Compositae) Iris odaesanensis(Iridaceae), Leontice microrhyncha(Berberidaceae), Rpdgersia tabularis(Saxifragaceae), and Acanthopanax sentico년(Araliaceae), Among them the four species were recorded from the list of the Ministry of Environment. As most of the species were recorded from the mountain-trails, the long-term habitat monitoring for the species is required .