• Title/Summary/Keyword: Light irradiation

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Gene Expression Analysis of Zeaxanthin Epoxidase from the Marine Microalga Dunaliella tertiolecta in Response to Light/Dark Cycle and Salinity

  • Kim, Minjae;Kang, Yongsoo;Jin, EonSeon
    • Journal of Microbiology and Biotechnology
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    • v.29 no.9
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    • pp.1453-1459
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    • 2019
  • Zeaxanthin is an important pigment in the photo-protection mechanism of microalgae. However, zeaxanthin epoxidase, an enzyme involved in the accumulation and conversion of zeaxanthin, has not been extensively studied in microalgae. In this work, we report the expression pattern of zeaxanthin epoxidase in Dunaliella tertiolecta (DtZEP) at different light and diverse salinity conditions. To confirm the responsiveness to light conditions, the ZEP expression pattern was investigated in photoperiodic (16 h of light and 8 h of dark) and continuous (24 h of light and 0 h of dark) light conditions. mRNA expression levels in photoperiodic conditions fluctuated along with the light/dark cycle, whereas those in continuous light remained unchanged. In varying salinity conditions, the highest mRNA and protein levels were detected in cells cultured in 1.5 M NaCl, and ZEP expression levels in cells shifted from 0.6 M NaCl to 1.5 M NaCl increased gradually. These results show that mRNA expression of DtZEP responds rapidly to the light/dark cycle or increased salinity, whereas changes in protein synthesis do not occur within a short period. Taken together, we show that DtZEP gene expression responds rapidly to light irradiation and hyperosmotic stress. In addition, ZEP expression patterns in light or salinity conditions are similar to those of higher plants, even though the habitat of D. tertiolecta is different.

Comparative evaluation of photobiomodulation therapy at 660 and 810 nm wavelengths on the soft tissue local anesthesia reversal in pediatric dentistry: an in-vivo study

  • Ankita Annu;Sujatha Paranna;Anil T. Patil;Sandhyarani B.;Adhithi Prakash;Renuka Rajesh Bhurke
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.23 no.4
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    • pp.229-236
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    • 2023
  • Background: Local anesthesia has been reliably used to control pain during dental procedures and is important in pediatric dentistry. However, children occasionally complain of prolonged numbness after dental treatment, leading to several problems. Studies conducted to reverse the effect of local anesthesia using phentolamine mesylate and photobiomodulation therapy (PBM) are encouraging but limited. PBM is a type of light therapy that utilizes visible and near-infrared non-ionizing electromagnetic spectral light sources. Hence, this study used this modality to compare the reversal of local anesthesia at two different wavelengths. This study compared the effect of PBM at 660 and 810 nm wavelengths on the reversal of soft tissue local anesthesia using a diode LASER in pediatric dentistry. Method: Informed consent and assent were obtained, and the participants were then divided randomly into three groups of 20 children each: control group-without LASER irradiation, LASER irradiation at 660 nm, and LASER irradiation at 810 nm. Sixty children aged 4-8 years with deciduous mandibular molars indicated for pulp therapy were administered an inferior alveolar nerve block. After 45 min of injection, a duration that was similar to the approximate duration of treatment, they were exposed to 660- and 810-nm LASER irradiation according to their groups until reversal of local anesthesia was achieved. The control group did not undergo LASER irradiation. The reversal of the soft tissue local anesthetic effect was evaluated using palpation and pin prick tests every 15 min, and the LASER irradiation cycle continued until reversal of the soft tissue local anesthesia was achieved. Results: A significant reduction of 55.5 min (27.6%) in the mean soft tissue local anesthesia reversal time was observed after the application of 810 nm wavelength PBM and 69 min (34.7%) after 660 nm wavelength LASER irradiation. Conclusion: PBM with a 660 nm wavelength was more effective in reducing the mean soft tissue local anesthesia reversal duration, and thus can be used as a reversal agent for soft tissue local anesthesia in pediatric dentistry.

Effect of Light-emitting Diodes (LEDs) and Ventilation on the in vitro Shoot Growth of Eucalyptus pellita (Eucalyptus pellita의 기내(器內) 줄기생장에 미치는 LEDs (Light-emitting diodes) 및 환기처리(換氣處理) 효과)

  • Kim, Ji-Ah;Moon, Heung Kyu
    • Journal of Korean Society of Forest Science
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    • v.95 no.6
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    • pp.716-722
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    • 2006
  • Various light sources including LEDs (Light emitting diodes) affecting on shoot growth was examined using in vitro shoots of E. pellita. Generally, it appeared that ventilation treatment was the most important factor affecting on normal shoot growth, irrespective of irradiation sources. Ventilation resulted in better performance of the cultures under 100% blue LED radiation. These include better shoot growth, more number of leaves, more number of internodes, more number of axillary buds, and heavier dry matters. The highest total chlorophyll content was obtained under both cool-white fluorescent lamps and R5B5 (50% red LED + 50% blue LED). The value was $24.5{\mu}g/g$ and $20.1{\mu}g/g$, respectively. In addition, ventilation resulted in higher carotenoid content in all irradiation sources except 100% red LED radiation. In conclusion, shoot growth of E. pellita could be reached maximum by ventilation under R5B5 (50% red LED + 50% blue LED).

Construction of Strontium Titanate/Binary Metal Sulfide Heterojunction Photocatalysts for Enhanced Visible-Light-Driven Photocatalytic Activity

  • Yu, Yongwei;Yang, Qing;Ma, Jiangquan;Sun, Wenliang;Yin, Chong;Li, Xiazhang;Guo, Jun;Jiang, Qingyan;Lu, Zhiyuan
    • Nano
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    • v.13 no.11
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    • pp.1850130.1-1850130.12
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    • 2018
  • A novel strontium titanate/binary metal sulfide ($SrTiO_3/SnCoS_4$) heterostructure was synthesized by a simple two-step hydrothermal method. The visible-light-driven photocatalytic performance of $SrTiO_3/SnCoS_4$ composites was evaluated in the degradation of methyl orange (MO) under visible light irradiation. The photocatalytic performance of $SrTiO_3/SnCoS_4-5%$ is much higher than that of pure $SrTiO_3$, $SnCoS_4$, $SrTiO_3/SnS_2$ and $SrTiO_3/CoS_2$. The $SrTiO_3/SnCoS_4$ composite material with 5 wt.% of $SnCoS_4$ showed the highest photocatalytic efficiency for MO degradation, and the degradation rate could reach 95% after 140 min irradiation time. The enhanced photocatalytic activity was ascribed to not only the improvement of visible light absorption efficiency, but also the construction of a heterostructure which make it possible to effectively separate photoexcited electrons and holes in the two-phase interface.

Photochromism of Diarylethene-Doped Organic-Inorganic Hybrid Low Melting Glass (Diarylethene이 첨가된 저융점 유-무기 하이브리드 유리의 광변색 특성)

  • Kim, Ji-Kyung;Kim, Myeong-Jeong;Park, Sung-Je;Ryu, Bong-Ki
    • Journal of the Korean Ceramic Society
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    • v.43 no.1 s.284
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    • pp.28-32
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    • 2006
  • Diarylethene in photochromic materials was doped in organic-inorganic hybrid low-melting glasses were synthesized through a nonaqueous acid-base reaction process, which consists of network units including a small number of bridging oxides. The organic phase is a dichlorodimethylsilane while the inorganic phase is a tin(II) chloride. Diarylethene was incorporated into the glasses without any degradation of its functionality. The open form of diarylenthene, which is converted from the opening one upon UV-irradiation, is change to the closed form visible light-irradiation. The rate constant of the photochemical reaction is $31.78\times10^{-3}s^{-1}$ with 400 W UV lamp irradiation.

A Study on the Dyeing of Polyester Fabric by Microwave Heating(I) (Microwave 가열에 의한 Polyester직물의 염색에 관한 연구(I))

  • 서수정;임수경;김삼수
    • Textile Coloration and Finishing
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    • v.10 no.4
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    • pp.7-14
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    • 1998
  • Microwaves are high frequency radiation capable of generating very rapid, uniform and efficient heating of textile material. Microwave heated dyeing of polyester fabric was tried with different solvent systems, irradiation time and dye concentration. Microwave fixation methods were used with 100% water,30% urea, EG and DMF, respectively, pad-baths in which the padded fabrics were exposed over a heated or boiling water bath to maintain sufficient moisture content during irradiation. In order to ascertain the relation between the dyeing property of polyester fabric and the microwave irradiation condition caused by microwave heating, the K/S values and fastness properties of dyed fabrics such as light, washing and sublimation fastness were measured.

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Pattern Dyeing of Cationized Cotton Fabrics by Ultra Violet Rays Irradiation (자외선조사에 의한 Cation화 면직물의 문양염색)

  • Kim, In Hui;Lee, In Seok;Nam, Seong U
    • Textile Coloration and Finishing
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    • v.14 no.2
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    • pp.77-77
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    • 2002
  • Cotton fabrics dipped in cationic agent(3-chloro-2-hydroxypropyl trimethyl ammonium chloride) were irradiated with deep Ultra Violet Rays(UV), in the region of UV-C using the low pressure mercury lamp. The chemical changes of cationized cotton fabric surfaces were investigated by FT-IR analysis. The dyeabilities of the irradiated portions were investigated by dyeing with acid dyes. Deep UV(UV-C) irradiation broke O-H bonds in cotton fiber and oxidized the fibers. The dyeability of the UV irradiated portion were different from that of the portion not irradiated. Various pattern were gained from one bath dig dyeing by pattern mask. The tensile strengths of cotton fabrics were decreased in the UV irradiated portion. Washing fastness of cotton dyeings were good about 3∼4 grade and light fastness were fair about 2∼3 grade.

Pattern Dyeing of Cationized Cotton Fabrics by Ultra Violet Rays Irradiation (자외선조사에 의한 Cation화 면직물의 문양염색)

  • 김인회;이인석;남성우
    • Textile Coloration and Finishing
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    • v.14 no.2
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    • pp.1-8
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    • 2002
  • Cotton fabrics dipped in cationic agent(3-chloro-2-hydroxypropyl trimethyl ammonium chloride) were irradiated with deep Ultra Violet Rays(UV), in the region of UV-C using the low pressure mercury lamp. The chemical changes of cationized cotton fabric surfaces were investigated by FT-IR analysis. The dyeabilities of the irradiated portions were investigated by dyeing with acid dyes. Deep UV(UV-C) irradiation broke O-H bonds in cotton fiber and oxidized the fibers. The dyeability of the UV irradiated portion were different from that of the portion not irradiated. Various pattern were gained from one bath dig dyeing by pattern mask. The tensile strengths of cotton fabrics were decreased in the UV irradiated portion. Washing fastness of cotton dyeings were good about 3∼4 grade and light fastness were fair about 2∼3 grade.

Physical Methods for the Identification of Irradiated Food

  • Yang, Jae-Seung;Lee, Hae-Jung
    • Preventive Nutrition and Food Science
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    • v.3 no.2
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    • pp.203-209
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    • 1998
  • The development of methods for the identification of irradiated foods helps enforce national and international regulations on labelling to ensure the consumer's free choice to buy irradiated or unirradiated foods. and the availabilityof such methods may assist the promotion of international trade in irradiated food products and help prevent abuse of the technology. A number of approaches to determine the physical , chemical, microbiological and biological changes that occur in foods treated with ionizing radiation have been studied. However no single method is universally applicable. Among physical measurements, the leading methods of indentification are electron spin resonance (ESR) spectroscopy and thermoluminescence(TL). ESR is an established non-destructive method for the analysis of free radicals from their traps and TL is the emission of light from irradiated mineral extracts by heating. Viscosity of carbohydrate polymers by causing chain breaks by irradiation, measuring the impedance of potatoes and detection of gases produced radiolytically are promising techniques for identification purposes. Irradiated water-containing foods show significant supercooling when monitored with a differential scanning calorimeter (DSC), which can be applied to identifying irradiated ones.

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Visible-light Photocatalytic Activity of BiOCl/Bi3O4Cl Nanocomposites

  • Gao, Bifen;Chakraborty, Ashok Kumar;Yang, Ji-Min;Lee, Wan-In
    • Bulletin of the Korean Chemical Society
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    • v.31 no.7
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    • pp.1941-1944
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    • 2010
  • The heterojunction structures of BiOCl/$Bi_3O_4Cl$, exhibiting considerable visible-light photocatalytic efficiency, were prepared by a simple wet-chemical process at ambient condition. The prepared nanocomposites were characterized by XRD, TEM, and UV-visible diffuse reflectance spectra. Under visible light (${\lambda}\geq$420 nm) irradiation, BiOCl/$Bi_3O_4Cl$ exhibited an enhanced photocatalytic activity in decomposing 2-propanol (IP) in gas phase and salicylic acid (SA) in aqueous solution, whereas the bare BiOCl and $Bi_3O_4Cl$ showed negligible activities. It is deduced that the remarkable visible-light photocatalytic activity of the BiOCl/$Bi_3O_4Cl$ originates from the hole $(h^+)$ transfer between VB of the $Bi_3O_4Cl$ and BiOCl semiconductors.