• Title/Summary/Keyword: Light Stress

Search Result 937, Processing Time 0.027 seconds

Effects of Various Light Spectra on Physiological Stress and DNA Damage by Thermal Stress in Juvenile Rock Bream (Oplegnathus fasciatus)

  • Choe, Jong Ryeol;Shin, Yoon Sub;Choi, Ji Yong;Kim, Tae Hwan;Kim, Daehee;Choi, Cheol Young
    • Ocean and Polar Research
    • /
    • v.39 no.2
    • /
    • pp.107-114
    • /
    • 2017
  • In this study, we investigated the effects of light spectra on physiology stress and DNA damage in juvenile rock bream (Oplegnathus fasciatus) using light-emitting diodes (LEDs; green, 520 nm; red, 630 nm) at two intensities (0.25 and $0.5W/m^2$ ) with application of thermal stress (25 and $30^{\circ}C$). We measured the mRNA expression of heat shock protein 70 (HSP70) and the levels of plasma cortisol, glucose, aspartate aminotransferase (AspAT), and alanine aminotransferase (AlaAT). Additionally, DNA damage was measured using comet assays. Our findings showed that HSP70 mRNA expression and plasma cortisol, glucose, AspAT, and AlaAT levels were significantly higher after exposure to high temperatures and were significantly lower after exposure to green LED light. Thus, although high water temperatures induced stress in juvenile rock bream, green LED light inhibited stress. In particular, green LED light reduced stress and DNA damage to a greater degree than other light sources.

Photoinhibition Induced Alterations in Energy Transfer Process in Phycobilisomes of PS II in the Cyanobacterium, Spirulina platensis

  • Kumar, Duvvuri Prasanna;Murthy, Sistla D.S.
    • BMB Reports
    • /
    • v.40 no.5
    • /
    • pp.644-648
    • /
    • 2007
  • Exposure of algae or plants to irradiance from above the light saturation point of photosynthesis is known as high light stress. This high light stress induces various responses including photoinhibition of the photosynthetic apparatus. The degree of photoinhibition could be clearly determined by measuring the parameters such as absorption and fluorescence of chromoproteins. In cyanobacteria and red algae, most of the photosystem (PS) II associated light harvesting is performed by a membrane attached complex called the phycobilisome (PBS). The effects of high intensity light (1000-4000 ${\mu}mol$ photons $m^{-2}s^{-1}$) on excitation energy transfer from PBSs to PS II in a cyanobacterium Spirulina platensis were studied by measuring room temperature PC fluorescence emission spectra. High light (3000 ${\mu}mol$ photons $m^{-2}s^{-1}$) stress had a significant effect on PC fluorescence emission spectra. On the other hand, light stress induced an increase in the ratio of PC fluorescence intensity of PBS indicating that light stress inhibits excitation energy transfer from PBS to PS II. The high light treatment to 3000 ${\mu}mol$ photons $m^{-2}s^{-1}$ caused disappearance of 31.5 kDa linker polypeptide which is known to link PC discs together. In addition we observed the similar decrease in the other polypeptide contents. Our data concludes that the Spirulina cells upon light treatment causes alterations in the phycobiliproteins (PBPs) and affects the energy transfer process within the PBSs.

The Effect of Light on Amorphous Silicon Thin Film Transistors based on Photo-Sensor Applications

  • Ha, Tae-Jun;Park, Hyun-Sang;Kim, Sun-Jae;Lee, Soo-Yeon;Han, Min-Koo
    • 한국정보디스플레이학회:학술대회논문집
    • /
    • 2009.10a
    • /
    • pp.953-956
    • /
    • 2009
  • We have investigated the effect of light on amorphous silicon thin film transistors based photo-sensor applications. We have analyzed the instability caused by electrical gate bias stresses under the light illumination and the effect of photo-induced quasi-annealing on the instability. Threshold voltage ($V_{TH}$) under the negative gate bias stress with light illumination was more decreased than that under the negative gate bias stress without light illumination even though $V_{TH}$ caused by the light-induced stress without negative gate bias was shifted positively. These results are because the increase of carrier density in a channel region caused by the light illumination has the enhanced effect on the instability caused by negative gate bias stress. The prolonged light illumination led to the recovery of shifted VTH caused by negative gate bias stress under the light illumination due to the recombination of trapped hole charges.

  • PDF

The Relationship between Stress from Housewives' Attitudes toward Dietary Life and their Health (주부들의 식생활태도 유형에 따른 스트레스와 자기건강에 관한 연구)

  • Kim, Sun-Hee
    • Korean journal of food and cookery science
    • /
    • v.22 no.6 s.96
    • /
    • pp.783-791
    • /
    • 2006
  • The consciousness and eating habits of the housekeeper directly influence the stress level and health quality of the household occupants. In Korea, most housewives are in charge of dietary life. Accordingly, their attitudinal clusters toward dietary (eating) life were researched in relation to their stress and health. The research results showed that irregular eating habits cause stress. Therefore, it is necessary for housewives to have a regular eating habit. The results were as follows. The house wives' attitudes toward dietary life were categorized into 5 clusters: regular and speedy overeating, regular and frequent eating, regular and light eating, irregular and light eating, irregular and speedy overeating. The cluster of regular and frequent snacks was 24.3%. The cluster of irregular and speedy overeating caused the most stress. Especially the house wives' group belonging to the cluster of irregular and speedy overeating were under social and emotional stress, while the cluster of regular and light eating and the cluster of irregular and light eating were under concentration stress. The cluster of regular and frequent snacks and the cluster of irregular and light eating had negative effect on their health, while the cluster of regular and speedy overeating had negative effect on their family's health by their social stress.

Analysis of Stress-Induced Effect in Blue GaN-Based Light-Emitting Diodes (질화갈륨 기반 청색 고체 발광 다이오드에서의 스트레스 영향 해석)

  • Shim, Sang Kyun;Lee, June Key;Kim, Youngman
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.32 no.6
    • /
    • pp.444-447
    • /
    • 2019
  • It was proven that the light outputs of blue GaN-based light-emitting diodes (LEDs) was seriously influenced by the application of external stress. We have simulated the wave function overlap of an electron and hole, which are significantly reduced by the development of stress. Consequently, its internal quantum efficiency decreased from 67.0% to 37.5%. To experimentally investigate the effect of stress, we designed and prepared a special zig system. By applying external tensile stress to compensate for the compressive stress innately developed in Blue LEDs, it was found that the optical output was greatly enhanced from 83.1 mcd to 117.2 mcd at a current of 100 mA, an increase of approximately 41%. In contrast, when the compressive stress is developed more by external compressive stress, we observed that the light output power was reduced from 89.0 mcd to 80.7 mcd, a decrease of approximately 9.3%.

Light/Dark Responsiveness of Kinetin-Inducible Secondary Metabolites and Stress Proteins in Rice Leaf

  • Cho, Kyoung-Won;Kim, Dea-Wook;Jung, Young-Ho;Shibato, Junko;Tamogami, Shigeru;Yonekura, Masami;Jwa, Nam-Soo;Kubo, Akihiro;Agrawal, Ganesh Kumar;Rakwal, Randeep
    • Journal of Crop Science and Biotechnology
    • /
    • v.10 no.2
    • /
    • pp.112-116
    • /
    • 2007
  • Kinetin(KN) is an inducer of rice(Oryza sativa L.) defense/stress responses, as evidenced by the induction of inducible secondary metabolite and defense/stress protein markers in leaf. We show a novel light-dependent effect of KN-triggered defense stress responses in rice leaf. Leaf segments treated with KN(100 ${\mu}M$) show hypersensitive-like necrotic lesion formation only under continuous light illumination. Potent accumulation of two phytoalexins, sakuranetin and momilactone A(MoA) by KN that peaks at 48 h after treatment under continuous light is completely suppressed by incubation under continuous dark. Using two-dimensional gel electrophoresis we identified KN-induced changes in ribulose-1, 5-bisphosphate carboxylase/oxygenase, energy- and pathogenesis-related proteins(OsPR class 5 and 10 members) by N-terminal amino acid sequencing and mass spectrometry. These changes were light-inducible and could not be observed in the dark(and control). Present results provide a new dimension(light modulation/regulation) to our finding that KN has a potential role in the rice plant self-defense mechanism.

  • PDF

EFFECT OF ANTE-MORTEM STRESS ON POST-MORTEM CHANGES OF TITIN I (α-CONNECTIN) INTO TITIN II (β-CONNECTIN) AND NEBULIN IN THE LIGHT AND DARK MUSCLE OF TAIWAN COUNTRY CHICKEN

  • Lin, L.C.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.7 no.3
    • /
    • pp.405-411
    • /
    • 1994
  • Purified myofibrils were prepared from ante-mortem stress and control lots of Taiwan country chicken breast and thigh muscles at death and afler storage at $4^{\circ}C$ for 0, 1, 2, 3, and 7 days post-mortem. Sodium dodecyl sulfate polycrylamide gel electrophoresis (3.2%) and densitometer were used to examine the effect of ante-mortem stress and control storage of muscle on titin and nebulin. Results indicated that titin and nebulin were more rapidly degraded in the control and the ante-mortem stress light muscles than in the control and ante-mortem dark muscles of Taiwan country chicken. In contrast, nebulin was shown to be more resistance to degradation in the ante-mortem stress dark muscle than in the control light muscle.

Analysis of Psychological, Physiological(Heart Rate Variability) Response & Influence Consequent on Green Light Brightness (녹색광 밝기에 따른 심리·생리(심박변이) 반응 및 영향 분석)

  • Jung, Chan-Ung;Im, O-Youn;Lee, Jin-Sook
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
    • /
    • v.30 no.3
    • /
    • pp.9-20
    • /
    • 2016
  • This study compared the effect of green light consequent on maximum brightness 85 lx and intial brightness 15 lx, and its accompanying psychological, physiological response. 1) As a result of psychology(general stress evaluation, Symptom Checklist-90-Revision) evaluation, all stress evaluation items mostly showed a decrease, and they are comparatively positively evaluated in minimum brightness 15 lx comparing to maximum brightness 85 lx. 2) Physiological (Heart Rate Variability) evaluation result was included in stable range in all cardiovascular system variables after light stimulus in time of comparison with the standard range; in addition, the Heart Rate Variability evaluation result was found to be stable in all cardiovascular system variables except Low Frequency in minimum brightness 15 lx comparing to maximum brightness 85 lx. 3) Green light was found not to appear as a stimulating factor in the human body in time of space projection, being found to be effective in stability of mind and body, and reduction in stress. Thus, it was found that green light functioned as an emotional, relaxing tranquilizer by working on the sympathetic nervous system.

Compression Behavior of Wood Stud in Light Framed Wall as Functions of Moisture, Stress and Temperature

  • Park, Joo-Saeng;Lee, Jun-Jae
    • Journal of the Korean Wood Science and Technology
    • /
    • v.34 no.5
    • /
    • pp.19-28
    • /
    • 2006
  • There has been considerable research in recent times in light-timber med structures in fires. These structures have included horizontal (floor-like) panels in bending and walls under eccentric and approximately concentric vertical loading. It has been shown that compression properties are the most dominant mechanical properties in affecting structural response of these structures in fire. Compression properties have been obtained by various means as functions of one variable only, temperature. It has always been expected that compression properties would be significantly affected by moisture and stress, as well. However, these variables have been largely ignored to simplify the complex problem of predicting the response of light-timber framed structures in fire. Full-scale experiments on both the panels and walls have demonstrated the high level of significance of moisture and stress for a limited range of conditions. Described in this paper is an overview of these conditions and experiments undertaken to obtain compression properties as a functions of moisture, stress and temperature. The experiments limited temperatures to $20{\sim}100^{\circ}C$. At higher temperatures moisture vaporizes and moisture and stress are less significant. Described also is a creep model for wood at high temperatures.

Zygosaccharomyces rouxii Combats Salt Stress by Maintaining Cell Membrane Structure and Functionality

  • Wang, Dingkang;Zhang, Min;Huang, Jun;Zhou, Rongqing;Jin, Yao;Wu, Chongde
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.1
    • /
    • pp.62-70
    • /
    • 2020
  • Zygosaccharomyces rouxii is an important yeast that is required in the food fermentation process due to its high salt tolerance. In this study, the responses and resistance strategies of Z. rouxii against salt stress were investigated by performing physiological analysis at membrane level. The results showed that under salt stress, cell integrity was destroyed, and the cell wall was ruptured, which was accompanied by intracellular substance spillover. With an increase of salt concentrations, intracellular Na+ content increased slightly, whereas intracellular K+ content decreased significantly, which caused the increase of the intracellular Na+/K+ ratio. In addition, in response to salt stress, the activity of Na+/K+-ATPase increased from 0.54 to 2.14 μmol/mg protein, and the ergosterol content increased to 2.42-fold to maintain membrane stability. Analysis of cell membrane fluidity and fatty acid composition showed that cell membrane fluidity decreased and unsaturated fatty acid proportions increased, leading to a 101.21% rise in the unsaturated/saturated fatty acid ratio. The results presented in this study offer guidance in understanding the salt tolerance mechanism of Z. rouxii, and in developing new strategies to increase the industrial utilization of this species under salt stress.