• Journal of Life Science
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• v.15 no.4 s.71
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• pp.600-606
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• 2005

Neurofilament (NF) proteins constitute the major intermediate filament type in adult neurons. They are made up by the copolymerization of the neurofilament light (NF-L, 61 kDa), medium (NF-M, 90kDa), and heavy (NF-H, 115 kDa) proteins. Although neurofilaments play a crucial .ole in neuronal growth, organization, shape, and plasticity, their expression pattern and cellular distribution in the developing neurons remain unknown. In this study, we have produced a rabbit polyclonal antibody specific to NF-M and investigated expression of NF-M in cultured cortical neurons. Immunostaining of 12 and 24 h cultures revealed strong expression of NF-M in axonal growth cone and in the region of a soma toward the axon. Doublestaining of 4 and 14 DIV corical neurons with NF-M and PSD95 antibodies revealed that both axon and dendrites were stained intensely with NF-M antibody, and that NF-M immunostaining along dendrites is often punctate and colocalize with PSD95 puncta, indicating that the puncta represent postsynaptic spines. Presence of NF-M in the postsynaptic spine was also indicated by immunoblot analysis of the postsynaptic density fraction. Taken together, our results show intensive targeting of NF-M into axons in the early axonal development, and into spines in mature neurons, indicating its important functions in axon and spine development.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.204-212
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• 2016

The soils used as trackbed in Korea are selected using USCS utilizing basic soil properties such as Grain Size Distribution(GSD), % passing of #200 sieve ($P_{200}$), % passing of #4 sieve ($P_4$), Coefficient of uniformity ($C_u$), and Coefficient of curvature ($C_c$). Degree of compaction of the soils adapted in the code by KR should be evaluated by maximum dry density (${\gamma}_{d-max}$) and deformation modulus $E_{v2}$. The most important influencing factor that is critical to stability and deformation of the compacted soils used as trackbed is stiffness. Thus, it is necessary to construct a correlation between the modulus and the basic soil properties of trackbed soil in order to redefine a new soil classification system adaptable only to railway construction. To construct the relationship, basic soil test data is collected as a database, including GSD, maximum dry unit weight (${\gamma}_{d-max}$), OMC, $P_{200}$, $P_4$, $C_u$, $C_c$, etc.; deformation modulus $E_{v2}$ and $E_{vd}$ are obtained independently by performing a Repeated Plated Bearing Test (RPBT) and Light Weight Deflectometer Test (LWDT) for ten different railway construction sites. A linear regression analysis is performed using SPSS to obtain the relationship between the basic soil properties and the deformation modulus $E_{v2}$ and $E_v$. Based on the constructed relationship and the various obtained mechanical test data, a new soil classification system will be proposed later as a guideline for the design and construction of trackbed foundation in Korea.

• The Korean Journal of Mycology
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• v.22 no.1
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• pp.107-116
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• 1994

Interorder somatic hybrids were obtained by protoplast fusion between Pleurotus ostreatus in the order Agaricales and Elfvingia applanata in the order Aphyllophorales. The fusants were classified into stable heterokaryons and spontaneously segregated heterokaryons. Hyphae of all fusion products except two strains did not form clamp connections. Out of them, two clamped and three clampless fusants produced mature fruiting bodies by light-dark cycle on sawdust rice bran medium. All of these basidiocarps had clamp connections. Three fusants were analysed with the distribution of progenies and segregation of genetic characters by random spore analyses. The genetic markers were shown to segregate and recombine in the first generation of monospores isolated from basidiocarps. Phenotypes of a large number of auxotrophic progenies were not detected in the two clamped fusants. The aberration ratio of segregants indicated the gene interaction resulting from different genome structure between distantly related species. The polymerase chain reaction (PCR) was adopted for the detection of somatic hybrids nuclear DNA. Four fusants showed a positive results in three kinds of primers. The prominent reaction products are represented by new bands in primer # 87 and # 125. Out of four fusants, two somatic hybrids had non-parental mtDNA patterns when digested with EcoR1 and HindIII. Comparison of somatic hybrids, tissue culture isolates(TC) and multispore germination isolates(MS) were made using esterase isozyme analysis. It is apparent that somatic hybrids had a minor banding patterns which are quite different from those of parents.

• Journal of the Korean Institute of Gas
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• v.20 no.6
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• pp.9-15
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• 2016

Liquefied petroleum gas (LPG) and compressed natural gas (CNG) are often used as fuel for vehicles because they are clean alternative gas fuels. CNG, as a low-carbon fuel, can contribute to the reduction of greenhouse gas emissions. LPG is often used as fuel for taxis because the performance is almost the same as that of gasoline but the price is lower. In the present study, the exhaust gas and the particle number (PN) of particulate matter, which is a recent environmental issue, were compared between LPG and CNG for the same vehicle. A chassis dynamometer was used to conduct the test according to the Federal Test Procedure (FTP)-75 and Worldwide harmonized Light-duty vehicle Test Procedure (WLTC) modes. The PN values of discharged particles having sizes of 5 nm or larger and 23 nm or larger were measured using two condensation particle counters (CPC). The ratio of carbon dioxide was high in the exhaust gas from the LPG vehicle; the ratio of methane was high in the exhaust gas from the CNG vehicle. The PN values of the emitted particles from the two fuels were similar. The PN values of particles having sizes of 23 nm or smaller were high in the high-speed WLTC mode.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.824-827
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• 2011

Needs for Smart Grid development are increasing all over the world as a solution to its problem according to depletion of energy resources, climatic and environmental rapidly change and growing demand for electrical power. Especially decentralized power is attracting world's attention. In this mood a new era for a unit scale of decentralized power environment is on its way in building. However there is a problem to have to be solved in the uniformity of power quality because the amount of power generated from renewable energy resources such as wind power and solar light is very sensitive to climate fluctuation. And thus this paper tries to suggest an energy management algorithm on basis of real time monitoring for meteorological data. The proposed EMS model embodies the method for predicting the power generation by monitoring and analyzing the climatic data and controling the efficient power distribution between the renewable energy and the existing power. The ultimate goal of this paper is to provide the technological basis for achieving zero-energy house.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.684-692
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• 2016

In the manufacturing process of the LQFP/TQFP (Low-profile Quad Flat Package/Thin Quad Flat Package), the requirement of a 3 dimensional inspection is increasing rapidly and a 3D inspection of the shape of a chip has become an important report of quality control. This study developed a 3 dimensional measurement system based on PMP (Phase Measuring Profilometry) for an inspection of the LQFP/TQFP chip and image processing algorithms. The defects of the LQFP/TQFP chip were classified according to the dimensions. The 2 dimensional optical system was designed by the dorm illumination to achieve constant light distribution, In the 3 dimensional optical system, PZT was used for moving 90 degree in phase. The problem of 2 ambiguity was solved from the measured moir? pattern using the ambiguity elimination algorithm that finds the point of ambiguity and refines the phase value. The proposed 3D measurement system was evaluated experimentally.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.288-294
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• 2013

Recently, sustainable production of biofuel using algal biomass is being pursued because of its enormous potential. First and foremost, securing superior strains to develop an efficient production system for algal biodiesel through screening or genetic improvement of microalgae is necessary. The genus of Botryococcus is regarded as one of the superior microalgae for biodiesel production due to its ability to accumulate high amounts of lipids and hydrocarbons. However, its low growth rate is a bottleneck for large-scale production and commercialization. The purpose of this study is to obtain indigenous Botryococcus strains which possess high lipid content and biomass productivity. The Botryococcus sp. was isolated from the Seobu Reservoir in Jeju Island and identified as Botryococcus sudeticus J2 by comparative analysis of 18s rRNA gene and ITS regions. The biomass productivity and lipid content of B. sudeticus J2 were 0.116 g $L^{-1}day^{-1}$ and 40.1% of dry wt., respectively. This was higher than the value of B. braunii UTEX 572, which is widely regarded as a superior strain among Botryococcus species. The relatively high growth rate of B. sudeticus J2 was achieved under a light intensity of 240 ${\mu}mol$ photons $m^{-2}s^{-1}$ with ambient air spargingwhen compared to 120 ${\mu}mol$ photons $m^{-2}s^{-1}$ with 2% $CO_2$ supply. In summary, it is likely that the isolated B. sudeticus J2 can be used for the mass cultivation and biodiesel production.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.9
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• pp.6221-6226
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• 2015

In this paper, waveguide analysis was interpreted as an optical waveguide using the feedback perturbation method simple and easy to apply compared to the analysis method, while the other almost identical to the numerical calculation method. In addition, efficient coupling with an optical transmission line of optical fiber and the waveguide form the thin film of different functional elements is required in order to achieve the full optical communication system. However, problems arise, such as the light field (field) and the decrease of the access efficiency due to inconsistency in the distribution characteristics of the connection surface by the difference in size of the cross section thereof when connecting the optical fiber and the waveguide directly to the combination of a thin film. Therefore propose a new type of connector structure to increase the efficiency of the connection when connecting the optical fiber waveguide and the thin film was analyzed by applying a coupled mode theory, the binding efficiency of the modified contactor. And by diffusing Ti on the $LiNbO_3$ substrate and a wide range of applications in the manufacture of integrated optical material made of a current low-loss Ti: $LiNbO_3$ optical waveguide and making the Y-branch waveguide, and the properties were confirmed.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2010.05a
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• pp.871-874
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• 2010

Needs for Smart Grid development are increasing all over the world as a solution to its problem according to depletion of energy resources, climatic and environmental rapidly change and growing demand for electrical power. Especially decentralized power is attracting world's attention. In this mood a new era for a unit scale of decentralized power environment is on its way in building. However there is a problem to have to be solved in the uniformity of power quality because the amount of power generated from renewable energy resources such as wind power and solar light is very sensitive to climate fluctuation. And thus this paper tries to suggest an energy management method on basis of real time monitoring for meteorological data. In the current situation of lacking in USN-based killer application in Smart Grid field, this paper proposes the USN-based DER management system which collects the meteorological data and control power system througout utilizing wireless sensor network technique this business. This communication technique is regarded to be efficient in aspects of installation cost and tits maintenance cost. The proposed EMS model embodies the method for predicting the power generation by monitoring and analyzing the climatic data and controling the efficient power distribution between the renewable energy and the existing power. The ultimate goal of this paper is to provide the technological basis for achieving zero-energy house.

• Korean Journal of Materials Research
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• v.27 no.12
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• pp.705-709
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• 2017

The electronic and optical characteristics of molybdenum disulphide ($MoS_2$) film significantly vary with its thickness, and thus a rapid and accurate estimation of the number of $MoS_2$ layers is critical in practical applications as well as in basic researches. Various existing methods are currently available for the thickness measurement, but each has drawbacks. Transmission electron microscopy allows actual counting of the $MoS_2$ layers, but is very complicated and requires destructive processing of the sample to the point where it will no longer be useable after characterization. Atomic force microscopy, particularly when operated in the tapping mode, is likewise time-consuming and suffers from certain anomalies caused by an improperly chosen set point, that is, free amplitude in air for the cantilever. Raman spectroscopy is a quick characterization method for identifying one to a few layers, but the laser irradiation causes structural degradation of the $MoS_2$. Optical microscopy works only when $MoS_2$ is on a silicon substrate covered with $SiO_2$ of 100~300 nm thickness. The last two optical methods are commonly limited in resolution to the micrometer range due to the diffraction limits of light. We report here a method of measuring the distribution of the number of $MoS_2$ layers using a low voltage field emission electron microscope with acceleration voltages no greater than 1 kV. We found a linear relationship between the FESEM contrast and the number of $MoS_2$ layers. This method can be used to characterize $MoS_2$ samples at nanometer-level spatial resolution, which is below the limits of other methods.