• Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.18-25
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• 2010

Interest in plant-derived food additives has increased recently, with efforts to identify natural antioxidant sources to replace synthetic antioxidants. This study evaluated the antioxidant effects of organic solvent fractions of 80% methanol extracts from Pediastrum duplex and Dactylococcopsis fascicularis microalgae. Among the solvent fractions tested, the ethyl acetate and n-hexane fractions from P duplex effectively scavenged 79.8% and 74.5% of DPPH free radicals, respectively. The chloroform fraction from D. fascicularis showed the strongest $H_2O_2$ scavenging ability (49.7%). The greatest scavenging of hydroxyl radicals (73.1%) was exhibited by the 80% methanol extract from P duplex. Aqueous residue and ethyl acetate fraction from P duplex provided the strongest nitric oxide scavenging (57.7%) and metal chelating effect (82.1%), respectively. Chloroform and ethyl acetate fractions from P duplex and n-hexane fraction from D. fascicularis exhibited significantly greater inhibition of lipid peroxidation than the commercial antioxidants. These results suggested that P duplex and D. fascicularis microalgae having potential anti oxidative compounds with various properties could be utilized in the food and pharmaceutical industries.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.913-922
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• 2013

An agar-degrading bacterium was isolated from red seaweed (Gelidium amansii) on a natural seawater agar plate, and identified as Saccharophagus sp. AG21. The ${\beta}$-agarase gene from Saccharophagus sp. AG21 (agy1) was screened by long and accurate (LA)-PCR. The predicted sequence has a 1,908 bp open reading frame encoding 636 amino acids (aa), and includes a glycosyl hydrolase family 16 (GH16) ${\beta}$-agarase module and two carbohydrate binding modules of family 6 (CBM6). The deduced aa sequence showed 93.7% and 84.9% similarity to ${\beta}$-agarase of Saccharophagus degradans and Microbulbifer agarilyticus, respectively. The mature agy1 was cloned and overexpressed as a His-tagged recombinant ${\beta}$-agarase (rAgy1) in Escherichia coli, and had a predicted molecular mass of 69 kDa and an isoelectric point of 4.5. rAgy1 showed optimum activity at $55^{\circ}C$ and pH 7.6, and had a specific activity of 85 U/mg. The rAgy1 activity was enhanced by $FeSO_4$ (40%), KCl (34%), and NaCl (34%), compared with the control. The newly identified rAgy1 is a ${\beta}$-agarase, which acts to degrade agarose to neoagarotetraose (NA4) and neoagarohexaose (NA6) and may be useful for applications in the cosmetics, food, bioethanol, and reagent industries.

• The Korean Journal of Community Living Science
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• v.24 no.2
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• pp.251-258
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• 2013

Korean oil and honey Yukwa has been paid attention as formal cake for traditional national seasons' holiday and religious service. Quality of Yukwa, however, has been maintained arbitrarily by each Yukwa manufacturer. Since even same Yukwa had severe differences in size, weight, and pattern, it has given the negative effect to the consumer. Yukwa industries need to setup the quantitative quality specifications instead of qualitative ones to maintain the uniformity of Yukwa quality. Efficient and economical inspection and process control system should be developed. In developing quality standards of Yukwa, features which can measure quality quantitatively in real time should be properly chosen. Existing quality features such as acidity, oxidization, hardness, viscosity, and texture were measured by the chemical or physical base destructive methods. Many research and developments have been performed in investigating and analyzing chemical transition states of those quality features as environment or storage condition changes. Most methods, however, require either off-line or complex treatment or time consuming process of analysis in evaluating quality features. Consumer, however, selects products mostly based on the external features such as shape, size, and color. Therefore, critical visual quality features should be chosen and the efficient real time measurement system must be developed. In this paper, computer image acquisition and processing system were developed and software modules were developed to extract the quantitative data of those features in real-time. Computer image processing system will promote in maintaining uniform quality of Yukwa and establishing quality standards of Yukwa.

• Proceedings of the Korean Society of Life Science Conference
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• 2002.12a
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• pp.48-60
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• 2002

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the production of granulocytes, macrophages, and white blood cells. The effects of osmotic pressure on secretion of human GM-CSF into the culture medium were investigated in suspension cultures of transgenic tobacco cells. An increase in osmotic pressure caused by the addition of mannitol decreased the cell size index, with the effect being more pronounced when cells were measured wet rather than dry. Increased osmotic pressure enhanced the secretion of hGM-CSF. At 90 g/L mannitol, the maximum concentration tested, hGM-CSF was present in the culture medium at 980 ug/L. As the concentration of mannitol increased, the total amount of protein secreted also increased, but was disproportionately enriched in GM-CSF NaCl, another osmoticum, had very similar effects on cell growth and hGM-CSF production, but did not cause enrichment for hGM-CSF Additionally, protein-stabilizing polymer was added to culture broth to enhance stability of secreted recombinant protein. Finally, above two method were applied together to maximize the productivity.

• BMB Reports
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• v.42 no.11
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• pp.743-746
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• 2009

L-ascorbic acid (Vitamin C) and peptide are both useful compounds for collagen biosynthesis in cosmeceuticals (cosmetic and pharmaceutical fields). The instability of these compounds, however, limit their application in these industries. In this report, we describe the development of a novel compound, Stabilized Ascorbyl Pentapeptide (SAP), which physically is much more stable than L-ascorbic acid in water. The inhibitory effects of this SAP compound on tyrosinase and melanin synthesis is comparable to that of L-ascorbic acid. Importantly, the SAP compound displays no cytotoxicity at a high concentration (5 mM). The ability of SAP to promote collagen biosynthesis is greater than that of L-ascorbic acid or the KTTKS peptide alone. Considering the in vitro stability and functional effects, our data strongly suggest that the SAP compound is a good candidate not only as a cosmetic ingredient, but also as a wound healing agent.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.2
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• pp.75-82
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• 2016

The new climate regime for practical reduction in green house gas(GHG) emissions was launched in Paris at Dec. 2015. The Korea government would make various policies and plans in order to achieve the BAU 37 % emission reduction goals by 2030. In this study, the current situation and the possible corresponding methodology to the GHG emission reduction of Korea paper industry were investigated. Although the GHG emission reduction in KOREA paper industry has been successfully conducted compared with other industries until now, the more efforts for controlling GHG emission would be required to meet the new climate regime. The efficiency of various GHG reduction projects conducted by Korea paper companies was evaluated to find efficient way for GHG reduction. The certified methodologies of the external project based offset systems in Korea GHG emission trading scheme were also reviewed for providing the possible way to develope tailored methodology to the Korea paper industry.

• Journal of Distribution Science
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• v.18 no.2
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• pp.17-28
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• 2020

Purpose: Human life is increasingly improved, so human needs are also growing more and more. One of the increasingly demanding industries is the fashion market. Fashion is an industry that produces essential items for human life, so manufacturers always need to know how to improve the relationship with customers, and make them repurchase. The study aims to discover the relationship between the factors that create a relationship with customers and the repurchase intention of designed fashion products. Research design, data, and methodology: The mix research method is applied to achieve research objectives. The qualitative research via the in-depth interview with 11 experts, and the quantitative research via the survey with 467 respondents was done in Ho Chi Minh city, the most developed city in Vietnam. Results: The research results point out that the fashion designer reputation, social media marketing, and the fashion store atmosphere have the positive impact on the relationship between the customer and fashion store, as well as the intention to repurchase of designed fashion products. In particular, customer relationship also affects the repurchase intention of designed fashion products positively. Conclusions: The study also proposed some managerial implications to develop a relationship with the customer and repurchase behavior of the customer in the fashion industry.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.4
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• pp.564-569
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• 2009

Antioxidative activities, total phenolic compounds and the sulforaphane contents of cooked broccoli extracts were studied. Total phenolic compounds were determined to be 0.96 mg/g(flower) and 0.76 mg/g(stem) in fresh broccoli extracts. The total phenolic compounds of cooked broccoli extracts showed 0.92 (1 min), 0.79 (3 min), 0.67 (10 min) mg/g when a boiling process was used and 1.27 (1 min) mg/g when a steaming process was used. In the DPPH assay, the steam process showed the highest free radical scavenging capacities. Sulforaphane has been of increasing interest in the nutraceutical and phamaceutical industries due to its anti-cancer effect. Sulforaphane was isolated from fresh and boiled, steamed broccoli using dichloromethane as an extract solvent. The sulforaphane contents of fresh broccoli were higher in the flower (14.78 mg/kg) than in the stem (6.16 mg/kg). The sulforaphane content dramatically decreased after the boiling ($100{\pm}2^{\circ}C$) or steaming ($100{\pm}2^{\circ}C$) processes were used.

• Corrosion Science and Technology
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• v.7 no.4
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• pp.219-223
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• 2008

In 2006, as a means to minimize early corrosion failure of ships, thus to enhance marine safety, International Maritime Organization (IMO), proposed a mandatory regulation for Performance Standards for the Protective Coatings (PSPC) for ballast tanks of newly built ships to satisfy 15 years of target useful life. In this regulation, several unprecedented strict rules are adopted as minimum, mandatory requirements for protective coatings of ship's water ballast tanks, and all type of ships sailing international sea are subjected to this regulation which is to be effective as early as June of 2008. The PSPC addresses many technical issues in the areas of surface pretreatment (primary and secondary), coating materials, coating application procedure and inspection as well as necessary documentation. The PSPC rules are new and unproven concepts, which calls for rigorous incorporation of reality-based evidences currently available, since there are no practical experiences in terms of the validity of the PSPC rules. There has been much controversy surrounding these regulations and considerable effort has been made by both shipyards and ship owners alike to achieve a performance standard for ballast tank coatings, which is acceptable to all. In this paper, the background and overview of the PSPC rules are given, and several issues in the PSPC are reviewed as a base to achieve robustness of the proposed PSPC, which will serve as a means to minimize early corrosion and to ensure 15 year target useful life of ships.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.578-583
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• 2005

Cell-free culture broth of marine halophilic bacterium, Kordia algicida was shown to possess specific algicidal ability against red tide organism, Cochlodinium polykrikides. Physiochemical characteristics of algicidal material originated in the bacterial culture broth were analyzed that its molecular weight was estimated to a 3,000 dalton and it was stable in heat and pH treatment. The algicidal fraction against C. polykrikoides obtained from gel permeable chromatography contained high concentration of ammonium ion as analyzed by ICP/Mass spectrum. C. polykrikoides by the fraction was quickly lysed within 1 min. It was shown that the effective concentration for algicide against C. polykrikoides was over 1mM of ammonium chloride. On the other hand, other metal ions presented in the algicidal fraction showed no algicidal effect against C. polykrikoides. In additon, ammonium ion exhibited species-specific killing spectrum for two species of red tide organisms, C. polykrikoides and Gymnodinium sanguieum. Therefore, further researches on the killing mechanism against C. polykrikoides exerted by ammonium ion, and subsequent development of replaceable algicidal materials will perform to provide useful tools for the control of red tide.