• Title/Summary/Keyword: Life test method

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Purity Test of Radish Hybrid Seeds Using Randomly amplified Polymorphic DNA Marker

  • Oh, Sei-myoung;Soontae Kwon
    • Journal of Life Science
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    • v.11 no.1
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    • pp.65-67
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    • 2001
  • In order to develop a rapid and simple method for testing the purity of radish hybrid seeds using a procedure based on the PCR(Polymerase chain reaction), eighty random primers were screened with the genomic DNA extracted from five day old seedlings of inbred parent lines and their F1 hybrids. Two primers, HRM-02 (5'-GAGACCAGAC-3') and HRM-19(5'-TGAGGCGTGT-3'), generate reproducible unique PCR patterns which can identify each parent lines as well as their hybrids. In actual test of randomly selected hybrid seeds using the two marker primers, the purity tested by one primer was exactly same as that of other primer. It suggests that one marker primer selected in this experiment is enough for the purity test of radish hybrid seeds. We demonstrates the use of RAPD(randomly amplified polymorphic DNAs) markers to identify each of inbred parent lines and hybrids by rapid and simple method.

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A Study of Tool Development for Powerlessness of Elderly and the Degree of Elderly′s Powerlessness (노인의 무력감 측정도구 개발과 무력감 정도에 관한 연구)

  • 정승은
    • Journal of Korean Academy of Nursing
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    • v.29 no.6
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    • pp.1294-1303
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    • 1999
  • The purpose of this study was to develop a tool to assess the powerlessness and to measure the powerlessness of elderly. From the result of pre-test, twenty seven items were finally selected to survey the elderly's powerlessness. The questionnaires on the elderly's powerlessness were drafted so that such tool may be evaluated in accordance with the four point Likert Scale. The number of subjects is 1,150 with ages of 60 years or more and who live in a large city, a small and middle-sized city and a rural area. The subjects of the test and retest were 85 elderlies. Collected data were analyzed by utilizing SAS program with Cronbach's $\alpha$ and Pearson's correlation, factor analysis method and known group techniques, descriptive statics, t-test and ANOVA. The results from this study were summarized below:1. When the factor analysis method was applied for validity, the tool for powerlessness of elderly was separated into 5 factors: loss of self-confidence to deal with physical, emotional, social aspects of life; expulsion by others from meaningful human relate; perception that life is meaningless and time passes quickly; falling behind young people; being rejected by other people, having no influence on others. An application of the known group technique showed a significant difference with the result of the degree of elderly's powerlessness tools developed by the two groups, with illness and without. 2. In testing reliability, it was found that coefficient of test-retest was .9435(P<.0001) when the test-retest method was used as a test of stability, and that the alpha coefficient of internal consistency was .9141 over all items within the tool of elderly's powerlessness 3. In powerlessness of elderly, total mean is 2.8493. And in factor of powerlessness, the highest factor is falling behind young people (M=3.1713), the lowest factor is loss of self- confidence to deal with physical, emotional, social aspects of life(M=2.6080). 4. The results from the test for the degree of powerlessness, according to the subject's demographic variables, showed that there were significant differences between age, sex, marital status, educational level, religion, possession of house, job, monthly pocket money, health status, illness and a place of residence.

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Development of Uniaxial Tensile Test Method to Evaluate Material Property of Tungsten Carbide-Cobalt Alloys for Cold Forging Dies (냉간단조 금형 WC-Co합금의 인장시험방법 개발 및 물성평가)

  • Kwon, I.W.;Seo, Y.H.;Jung, K.H.
    • Transactions of Materials Processing
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    • v.27 no.6
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    • pp.370-378
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    • 2018
  • Cold forging, carried out at room temperature, leads to high dimensional accuracy and excellent surface integrity as compared to other forging methods such as warm and hot forgings. In the cold forging process, WC-Co (Tungsten Carbide-Cobalt) alloy is the mainly used material as a core dies because of its superior hardness and strength as compared to other structural materials. For cold forging, die life is the most significant factor because it is directly related to the manufacturing cost due to periodic die replacement in mass production. To investigate die life of WC-Co alloy for cold forging, mechanical properties such as strength and fatigue are essentially necessary. Generally, uniaxial tensile test and fatigue test are the most efficient and simplest testing method. However, uniaxial tension is not efficiently application to WC-Co alloy because of its sensitivity to alignment of the specimen due to its brittleness and difficulty in thread machining. In this study, shape of specimen, tools, and testing methods, which are appropriate for uniaxial tensile test for WC-Co alloy, are proposed. The test results such as Young's modulus, tensile strength and stress-strain curves are compared to those in previous literature to validate the proposed testing methods. Based on the validation of test results it was concluded that the newly developed testing method is applicable to other cemented carbides like Titanium carbides with high strength and brittleness, and also can be utilized to carry out fatigue tests for further investigation on die life of cold forging.

The Effects of Scapula and Pelvis Symmetrical Reciprocal Pattern of PNF Concept on Respiratory Function and Quality of Life in the Elderly (PNF의 어깨뼈 골반 대칭 상호 패턴이 노인의 호흡기능과 삶의 질에 미치는 효과)

  • Byoung-Hyoun Moon;Ji-Won Kim
    • Journal of The Korean Society of Integrative Medicine
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    • v.12 no.2
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    • pp.55-63
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    • 2024
  • Purpose : The purpose of this study is to determine the effects of scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept on respiratory function and quality of life in elderly subjects. Methods : Nineteen elderly subjects with healthy were recruited. Subjects performed scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept. Exercise was appied 30 minutes three times per week for four weeks. The respiratory function and quality of life (QOL) test three times (before, two weeks, and four weeks). Respiratory function includes forced vital capacity (FVC), forced expiratory volume at one second (FEV1), Peak Expiratory Flow (PEF) and chest cage expansion test (CCET). The QOL test was measured Korean WHOQOL-BRIEF. The analysis method was analyzed through the one-way ANOVA repeated methods, and the statistical significance is α=.05. It was analyzed through the post test Bonferroni test. Results : After the 4 week scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept showed statistically significant differences in the respiratory function (FVC, FEV1, PEF, and CCET), and QOL (p<.05). As a result of the post-hoc test, FVC showed a significant increase in the mid test and post test compared to the pre test (p<.05), FEV1 showed a significant increase in the post test compared to the pre test (p<.05). PEF showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05), CCET showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). QOL showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). Conclusion : In this study, the scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept improved respiratory function and QOL. The findings suggest that this intervention could be beneficial in improving respiratory function and QOL in the elderly.

Economic Design of Zero-Failure Reliability Qualification Test (경제적인 무고장 신뢰성 인증시험 설계)

  • Kwon, Young-Il
    • Journal of Korean Society for Quality Management
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    • v.39 no.1
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    • pp.71-77
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    • 2011
  • In the fields of reliability application, the most commonly used test methods for reliability qualification are zero failure tests since they require fewer test samples and less test time compared to other test methods that guarantee the same reliability with a given confidence level. An economic zero failure test plan is developed that minimizes the total cost related to perform a life test to guarantee a specified reliability of a product with a given confidence level and a numerical example is provided to illustrate the use of the proposed test method.

Evaluation for Fatigue Life of Rubber Isolator for Vibration Characteristic on Automotive Cooling Module (진동 특성을 고려한 자동차 냉각모듈 방진고무의 내구성 평가)

  • Shim, Hee-Jin;Kim, Han-Chul;Kim, Jung-Kyu
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.350-355
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    • 2008
  • A Rubber mount is widely used for mechanical parts or engineering materials. Especially, it plays an important role in reducing mechanical vibration due to cyclic loading. But, rubber mount is damaged due to the cyclic loading and resonance. Therefore, it is necessary to investigate evaluation of fatigue life considering vibration characteristics for rubber. In this study, a vibration fatigue analysis was performed and based on Power Spectral Density(PSD) and the stress-life curve and a result of frequency response analysis in the finite element method. The measured load history in experiment was transformed to PSD curve. The stress-life curve was obtained by nonlinear static analysis and fatigue test. In addition, frequency response analysis was conducted for mechanical part. In order to evaluate fatigue life of rubber mount, vibration fatigue test was conducted at the constant acceleration-level as well. Fatigue life was determined when the load capacity is reduced to 60% of its initial value. As a result, predicted fatigue life of rubber mount agreed fairly well with the experimental fatigue life.

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A Study on the Quality of Life and Sexual Function of Hematopoietic Stem Cell Transplantation Recipients (조혈모세포이식 대상자의 삶의 질과 성기능)

  • Jang, Jong-Hyun;Cho, Bok-Hee
    • Asian Oncology Nursing
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    • v.9 no.1
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    • pp.7-14
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    • 2009
  • Purpose: This study was to analyze the level of quality of life and sexual function of Hematopoietic Stem Cell Transplantation (HSCT) recipients. Method: Participants included 38 recipients of HSCT, attending hospital outpatient department. Data were collected using the quality of life scale and the sexual function scale. Data were analyzed using Descriptive statistics, t-test, ANOVA, Sheff$\acute{e}$ test, and Kruskal-Wallis test. Results: The high score of quality of life was found in the participants with the 30s age (p=0.030), being employed (p=0.001), not having admission history after HSCT (p=0.012), and not having medication (p=0.017). The sexual function was significantly influenced by employment status (p=0.001) and irradiation therapy (p=0.043). There was a significant correlation between the quality of life and the sexual function (r=0.45, p=0.004). Conclusion: The sexual function have influence on the quality of life so that it is required to develop nursing interventions for sexual function to improve the quality of life of HSCT recipients.

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Development of Immunochromatography Strip-Test Using Nanocolloidal Gold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain and Feed Samples

  • Shim, Won-Bo;Yang, Zheng-You;Kim, Jung-Sook;Kim, Ji-Young;Kang, Sung-Jo;Woo, Gun-Jo;Chung, Young-Chul;Eremin, Sergei A.;Chung, Duck-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.17 no.10
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    • pp.1629-1637
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    • 2007
  • An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

On Estimating the Parameters of an Extended Form of Logarithmic Series Distribution

  • Kumar, C. Satheesh;Riyaza, A.
    • Communications for Statistical Applications and Methods
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    • v.20 no.5
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    • pp.417-425
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    • 2013
  • We consider an extended version of a logarithmic series distribution and discuss the estimation of its parameters by the method of moments and the method of maximum likelihood. Test procedures are suggested to test the significance of the additional parameter of this distribution and all procedures are illustrated with the help of real life data sets. In addition, a simulation study is conducted to assess the performance of the estimators.

Material Degradation in KS D 3503 SS400 Rolled Steel at $179^{\circ}C$ (KS D 3503 SS400 압연강 $179^{\circ}C$에서의 재질열화 연구)

  • Baek, Un-Bong;Park, Jong-Seo;Nahm, Seung-Hoon
    • Journal of the Korean Society of Safety
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    • v.21 no.4 s.76
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    • pp.13-18
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    • 2006
  • In spite of frequent defect in industrial boilers, life assessment or diagnostic method for them has not been actively studied. In this research, SS400 carbon steel used in industrial boilers is simulated with artificial aging heat treatment. To do qualitative life assessment, differences in micro-structures and hardness of SS400 by the degradation time are studied. In addition, variation in material properties by aging was observed with the tensile test at room temperature and $179^{\circ}C$ and changes in ductile to brittle transition temperature was observed with the charpy impact test performed at several test temperature.