• Animal Bioscience
• /
• v.36 no.8
• /
• pp.1285-1292
• /
• 2023

Objective: The objective of this study was to develop a novel endolysin (PanLys.1) for the specific killing of the ruminal hyper-ammonia-producing bacterium Peptostreptococcus anaerobius (P. anaerobius). Methods: Whole genome sequences of P. anaerobius strains and related bacteriophages were collected from the National Center for Biotechnology Information database, and the candidate gene for PanLys.1 was isolated based on amino acid sequences and conserved domain database (CDD) analysis. The gene was overexpressed using a pET system in Escherichia coli BL21 (DE3). The lytic activity of PanLys.1 was evaluated under various conditions (dosage, pH, temperature, NaCl, and metal ions) to determine the optimal lytic activity conditions. Finally, the killing activity of PanLys.1 against P. anaerobius was confirmed using an in vitro rumen fermentation system. Results: CDD analysis showed that PanLys.1 has a modular design with a catalytic domain, amidase-2, at the N-terminal, and a cell wall binding domain, from the CW-7 superfamily, at the C-terminal. The lytic activity of PanLys.1 against P. anaerobius was the highest at pH 8.0 (p<0.05) and was maintained at 37℃ to 45℃, and 0 to 250 mM NaCl. The activity of PanLys.1 significantly decreased (p<0.05) after Mn²⁺ or Zn²⁺ treatment. The relative abundance of P. anaerobius did not decrease after administration PanLys.1 under in vitro rumen conditions. Conclusion: The application of PanLys.1 to modulate P. anaerobius in the rumen might not be feasible because its lytic activity was not observed in in vitro rumen system.

• Food Science of Animal Resources
• /
• v.41 no.4
• /
• pp.739-747
• /
• 2021

This study examined the measurement conditions of the texture profile analysis (TPA) experiments that are typically used to measure the physical properties of sausage. As the measurement conditions (compression ratio and cross-head speed) were changed, the significant differences between the values of textural parameters (hardness, adhesiveness, springiness, cohesiveness, chewiness) of samples changed. Furthermore, among the measurement conditions, there was more variation in the significant difference between the value of samples due to a change in the compression ratio than due to a change in the cross-head speed. The highest variation in significant difference was observed between the values of cohesiveness of samples due to changes in measurement conditions, whereas the lowest variation in significant difference was observed between the values of springiness of samples due to change in measurement conditions. Therefore, it is desirable to provide a clear basis for setting specific measurement conditions for TPA test, since significant differences in the values of textural parameters of samples were caused by differences in cross-head speed or compression ratio, not by a difference in samples, when analyzing the cohesiveness of sausage, especially.

• Korean Journal of Adult Nursing
• /
• v.19 no.1
• /
• pp.12-23
• /
• 2007

Purpose: The purpose of the present study was to explore the meaning of quality of life in patients with chronic cardiovascular disease. Methods: A grounded theory method guided data collection and analysis. A total of 16 adult outpatients with chronic cardiovascular disease was participated. Data were collected through individual in-depth interviews. All interviews were audio taped and transcribed verbatim. Coding was used to establish different concepts and categories. A theoretical sampling technique was used to obtain diverse data from many relevant categories. Results: Seven categories were extracted, and they divided into constructing and intervening factors of quality of life. Constructing factors were uncertainty, recovery in the sense of control and maintaining social life. Intervening factors were symptom experiences, social support, taking care of themselves, and reflecting life. 'Keeping restrictive conditions under control' was emerged as a core category. The meaning of quality of life in patients with cardiovascular was explained according to the levels of keeping restrictive conditions under control. Conclusion: The result of this study may contribute for health professionals to understand the quality of life in patients with cardiovascular disease.

• The Journal of Industrial Distribution & Business
• /
• v.8 no.5
• /
• pp.35-42
• /
• 2017

Purpose - This study investigated small business members' cognition of happiness in accordance with changes of employment environment at the nation's industrialization. The study verified effects of labor workers' psychological wellbeing conditions upon life satisfaction as well as the effects of job burden and family burden of employment instability. Research design, data, and methodology - The subjects were small business workers in Seoul and Metropolitan areas. The author collected 150 copies of effective questionnaires. Regression analysis, hierarchical analysis and 3-stage mediation regression analysis were done. Results - The findings of this research was that psychological wellbeing factors alleviated job burden and family burden to increase satisfaction with life. Employment instability moderated between job burden and satisfaction with life, job burden between environmental control and satisfaction with life, and family burden between goal and satisfaction of life. Members needed sub factors of psychological wellbeing, especially reflection for self-acceptance. Conclusions - This study inspected effects of small business workers' psychological wellbeing upon life satisfaction at changes of management environment as well as labor environment to verify effects of job burden, family burden and employment instability and to find out conditions of human resources management from point of view of small business and workers.

• Nuclear Engineering and Technology
• /
• v.36 no.5
• /
• pp.475-484
• /
• 2004

Extending the lifetime of a nuclear power plant [(hereafter referred to simply as NPP)] is one of the most important concerns in the global nuclear industry. Cables are one of the long-life items that have not been considered for replacement during the design life of a NPP. To extend the cable life beyond the design life, it is first necessary to prove that the design life is too conservative compared with actual aging. Condition monitoring is useful means of evaluating the aging condition of cable. In order to simulate natural aging in a nuclear power plant. a study on accelerated aging must first be conducted. In this paper, evaluations of mechanical aging degradation for a neoprene cable jacket were performed after accelerated aging under tcontinuous and intermittent heating conditions. Contrary to general expectations, intermittent heating to the neoprene cable jacket showed low aging degradation, 50% break-elongation, and 60% indenter modulus, compared with continuous heating. With a plant maintenance period of 1 month after every 12 or 18 months operation, we can easily deduce that the life time of the cable jacket of neoprene can be extended much longer than extimated through the general EQ test. which adopts continuous accelerated aging for determining cable life. Therefore, a systematic approach that considers the actual environment conditions of the nuclear power plant is required for determining cable life.

• Journal of Preventive Medicine and Public Health
• /
• v.55 no.5
• /
• pp.444-454
• /
• 2022

Objectives: We aimed to identify the factors related to depression and quality of life in patients with hypertension by using multilevel regression analysis. Methods: In 2019, 229 043 participants in the Korean Community Health Survey were selected as the study group. Individual factors were identified using data from the 2019 Community Health Survey. Regional factors were identified using data from the National Statistical Office of Korea. Multilevel regression analysis was conducted to find individual and local factors affecting depression and quality of life in patients with hypertension and to determine any associated interactions. Results: As individual factors in patients with hypertension, women, those with lower education-levels, recipients of basic livelihood benefits, and those with poor dietary conditions showed stronger associations with depression and quality of life. As regional factors and individual-level variables in patients with hypertension, lower gross regional personal income, fewer doctors at medical institutions, and lower rates of participation in volunteer activities presented stronger associations with depression and quality of life. In addition, the associations of depression with gross regional personal income, the number of doctors at medical institutions, and dietary conditions were significantly stronger in patients with hypertension than in patients without hypertension. The associations of gender and employment status with quality of life were also significantly greater. Conclusions: Policy interventions are needed to adjust health behaviors, prevent depression, and improve quality of life for patients with hypertension, especially for those with the risk factors identified in this study.

• The Korean Journal of Food And Nutrition
• /
• v.24 no.3
• /
• pp.295-305
• /
• 2011

The goal of this study was to provide basic data for the future development of dietary life education in elementary schools by understanding the current conditions of the program after the execution of the Dietary Life Education Support Act. We conducted a survey to understand dietary teachers' recognition of dietary life education in elementary schools. We found that teachers' recognition was low and that dietary life education in elementary schools is still carried out indirectly by focusing on nutrition knowledge. Dietary life education is carried out during lunch time and dietary life-related class hours. However teachers of lower class levels did not have any practical subject related to dietary life and responded that a lack of time devoted to dietary life education was the biggest problem with the program. Most teachers responded that dietary life education in elementary schools is necessary for the formation of students' sound dietary habits, and that the development of a system of teachers directly responsible for dietary life education is necessary. In the past, dietary life education has typically been carried out at home, but now schools are required to play a role because of family nuclearization and the increase in working couples. Therefore, to revitalize dietary life education programs in elementary schools according to the Dietary Life Education Support Act, we suggest more systematic and segmentalized studies.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2018.10a
• /
• pp.262-262
• /
• 2018

• Journal of Biosystems Engineering
• /
• v.41 no.1
• /
• pp.1-11
• /
• 2016

Purpose: The gear strength of a new mechanical transmission designed to increase the loading weight of small 4.8 kW tracked agricultural transporters was analyzed. Design improvements to increase the gear strength and reduce the gear weight were proposed after examining the parameters. Methods: Sixteen operators from three regions were surveyed to obtain the usage profile of small 4.8 kW transporters. Gear strength was evaluated by calculating contact stress and tooth root stress using commercial software following ISO 6336. Results: From the strength calculation for each gear pair, contact stress smaller than tooth root stresses were produced in all gear pairs. The safety factors in most cases exceeded 1.0, except in the case of gear pair II in group II. The design life of the transporter using gear pair II in group II was 42% under harsh conditions-thus, this design life needs improvement. A robust design was proposed by examining the relevant parameters (face width and profile shift coefficient) to increase the design life of the transporter. In addition, a lightweight design for gear pair I in group II that was considered overdesigned was proposed by examining the face width to reduce the weight of the drive gear by 42% and that of the driven gear by 30%. Conclusions: The Safety factor for the design life was examined through a gear strength analysis. After examining the relevant parameters, conditions for strength improvement were proposed to increase design life or adjust overdesigned gear. However, load conditions differ depending on the working conditions or user's preferences; therefore, it is necessary to conduct further studies in various regions.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.11
• /
• pp.1868-1874
• /
• 2007

We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns${\times}$six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handler is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins.