• Journal of Life Science
• /
• v.33 no.10
• /
• pp.842-850
• /
• 2023

Pyrrolnitrin, pyrrolomycin, and pyoluteorin are functional halogenated phenylpyrrole derivatives (HPDs) derived from microorganisms with diverse antimicrobial activities. Pyrrolnitrin is a secondary metabolite produced from L-tryptophan through four-step reactions in Pseudomonas fluorescens, Burkholderia cepacia, Serratia plymuthica, etc. It is currently used for the treatment of superficial dermatophytic fungal infections, has high antagonistic activities against soil-borne and foliar fungal infections, and has many industrial applications. Since pyrrolnitrin is easily decomposed by light, it is difficult to widely use it outdoors. As an alternative, fludioxonil, a synthetically produced non-systemic surface fungicide that is structurally similar and has excellent light stability, has been commercialized for seed and foliar treatment of plants. However, due to its high toxicity to aquatic organisms and adverse effects in human cell lines, many countries have established maximum residue levels and strictly control its levels. Pyrrolomycin and pyoluteorin, which have antibiotic/antibiofilm activity against Gram-positive bacteria and high anti-oomycete activity against the plant pathogen Pythium ultimum, respectively, were isolated and identified from microorganisms. This review summarizes the biosynthesis and production of natural pyrrolnitrin derived from bacteria and the characteristics of synthetic fludioxonil and other natural phenylpyrrole derivatives among the HPDs. We expect that a plethora of highly effective, novel HPDs that are safe for humans and environments will be developed through the generation of an HPD library by microbial biosynthesis and chemical synthesis.

• Journal of the Korean Society for information Management
• /
• v.30 no.4
• /
• pp.61-91
• /
• 2013

In order to achieve effective human resource development in LIS fields, it is essential to have strategies to nurture and utilize those human resources, in relation to supply and demand as well a sound legal foundation supporting those strategies. Such strategies and legal foundation can only be developed from a complete knowledge of the current status of human resources in the LIS and related industries. This study, therefore, conducted research on the basic employment status, employment environment, and an overall analysis of related issues, in order to address policy implications on the effectiveness of human resource development in the LIS field. This research included the current status of policy environments that involve social environment, and related institutions and laws, and human resources developments as well as the current requirements of librarians in the field, based on a demand survey of LIS employment. It was found that first, there are three distinguishable factors in LIS employment: a feminization of librarianship, an increase in temporary posts, and a high entering ratio into the library workplace. Second, while there were only little differences in the given tasks between full-time and temporary employees, the differences in salary and welfare were considerably larger. Third, field requirements for librarian education included a mentoring system with field experts, short-term internships, and librarian apprenticeships, while job requirements included internship or apprenticeship, language skills, various license acquisition, and career management. Fourth, librarians with licenses for related organizations held 20% more librarian licenses overall.

• Journal of Acupuncture Research
• /
• v.28 no.4
• /
• pp.57-64
• /
• 2011

Objectives : The aim of this study is to overview the current status of Cochrane systematic review of acupuncture. Methods : Published Cochrane systematic reviews and protocols for evaluating acupuncture and/or moxibustion were searched by the terms 'acupuncture' and 'moxibustion' in Issue 1, Cochrane Library, 2011. Only reviews or protocols that regarded acupuncture or moxibustion as primary methods of intervention in their reviews were included. Data regarding first author national affiliation, intervention and disease, as well as the number of reviews or protocols that included Korean, Chinese, or Japanese databases in search strategies were obtained. Results : We found 92 hits at screening, and 59 studies were eligible for this review. Included interventions were acupuncture (n=56), auricular acupuncture (n=2), and moxibustion (n=1) for various diseases and conditions. The number of publications of Cochrane systematic reviews or protocols increased gradually from 2002 to 2011. First authors' affiliations were located in China (n=32), Australia (n=8), UK (n=7), Canada (n=4), U.S. (n=4), Germany (n=2), Norway, (n=1) and Korea (n=1). 44, four and four Cochrane reviews adopted Chinese, Korean and Japanese databases, respectively. Conclusions : Search strategies in a substantial number of Cochrane reviews or protocols for acupuncture and moxibustion heavily relied on Chinese databases. Korean databases were underused in existing Cochrane reviews and protocols, and Korean authors were much less involved in the Cochrane systematic reviews or protocols than Chinese authors. Further studies need to be conducted in order to incorporate evidence of acupuncture reported in Korean databases into the Cochrane systematic reviews.

• Archives of design research
• /
• v.18 no.1 s.59
• /
• pp.105-114
• /
• 2005

With the upcoming Digital age, it is time to develop digitalized contents based on cultural basis. in this respect, Kimchi is one of the most primal cultural content signifying Korean culture. It's no doubt the time to initiate the movement to digitalize the contents concerning Kimchi by gathering materials on the origin of Kimchi culturally and a great value to revive digital contents regarding Kimchi. Therefore, in this research, it is regarded that Kimchi is one of the most prominent cultural value that is possible to inherit from generations and should be stratified on the database. A method explaning Korean Kimchi culture is sought in this paper. And It is focused on the culture of Korean Kimchi profoundly and focus on detailed context and varied styles of he digital contents. It is organized on the basis of various aged volumes regarding Kimchi classified by seasons, ingredients, Jonga Kimchi (Kimchi inherited from the oldest family by generations), 24 seasonal divisions of the year, and 8 regional divisions of the nation. It is planned to set up digitally-focused library regarding cultural origin of contents and to present solidity and a feeling of being dynamic The results could be used in a variety of Industries in tourism, education, Kimchi and building basic infrastructure in entertainment industry. It is also applied to specialized industry by providing cultural contents and it could contribute to activation of Kimchi industry with e-Market and portal site regarding Kimchi. Moreover, it may re-establish the cultural value of Kimchi and provide the infrastructure in Kimchi-related industry such as Kimchi cyber museum, Kimchi Expo, Kimchi town by using educational materials in Education industry fields (regular curriculum and experience tour).

• Journal of Life Science
• /
• v.15 no.6 s.73
• /
• pp.851-856
• /
• 2005

Although valuable microbes have been isolated from the soil for the various productions of useful components, the microbes which can be cultivated in the laboratory are only $0.1-1\%$ of all microbes. To solve this problem, the study has recently been tried for making the valuable components from the environment by directly separating unculturable micrbial DNA in the soil. But it is known that humic acid originated from the soil interrupts various restriction enzymes and molecular biological process. Thus, in order to prevent these problems, this study modified the method separated soil DNA with phenol, CTAB and PEG. In order to compare the degree of purity for each DNA and the molecular biological application process, $A_{260}/A_{280}$ ratio, restriction enzymes, and PCR were performed. In case of DNA by the modified method, total yield of DNA was lower but $A_{260}/A_{280}$ ratio was higher than the previously reported methods. It was confirmed that the degree of purity is improved by the modified method. But it was not cut off by all kinds of tested restriction enzymes because of the operation of a very small amount of interrupting substances. When PCR was operated with each diluted DNA in different concentrations and GAPDH primer, the DNA by the modified method could be processed for PCR in the concentration of 100 times higher than by the previously reported separation method. Therefore, this experiment can find out the possibility of utilization for the unknown substances by effectively removing the harmful materials including humic acid and help establishing metagenomic DNA library from the soil DNA having the high degree of purity.

• Therapeutic Science for Rehabilitation
• /
• v.12 no.2
• /
• pp.43-53
• /
• 2023

Objective : In this study, the rehabilitation motivation evaluation tool for stroke patients was identified, and the characteristics of the evaluation tool were analyzed to analyze the conceptual framework and characteristics used in the composition of the evaluation contents. Methods : From 2000 to May 2022, documents registered in major domestic and foreign databases (PubMed, NDSL, Medline, and EMbase) were searched and selected according to the selection and exclusion criteria. The evaluation tools used in the selected documents were analyzed, and the characteristics of this evaluation tool were analyzed. Results : A total of 30 documents were selected, and they were classified into a total of five rehabilitation motivation evaluation tools. The most widely used in Korea was the measurement tool for rehabilitation motivation for the disabled, and the most widely used overseas was the intrinsic motivation inventory. Three evaluation tools constructed the evaluation contents based on the self-determination theory, and two constructed the evaluation contents through expert agreement. The number of evaluation items and 8 to 45 items were varied, and the evaluation scale was also used in various ways from 4 to 7 point scale. Conclusion : It was possible to identify the evaluation tools mainly used for the evaluation of rehabilitation motivation, and to identify the main conceptual framework based on the development of the rehabilitation motivation evaluation tool. The results of this study are expected to be used as basic data for research on the development of rehabilitation motivation evaluation tools for stroke patients in the future.

• Journal of Internet of Things and Convergence
• /
• v.10 no.2
• /
• pp.85-91
• /
• 2024

In this paper, we studied projection mapping, which is being utilized as a digital canvas beyond space and time for theme parks, mega events, and exhibition performances. Since the existing projection technology used for fixed objects has the limitation that it is difficult to map moving objects in terms of utilization, it is urgent to develop a technology that can track and map moving objects and a real-time dynamic projection mapping system based on dynamically moving objects so that it can respond to various markets such as performances, exhibitions, and theme parks. In this paper, we propose a system that can track real-time objects in real time and eliminate the delay phenomenon by developing hardware and performing high-speed image processing. Specifically, we develop a real-time object image analysis and projection focusing control unit, an integrated operating system for a real-time object tracking system, and an image processing library for projection mapping. This research is expected to have a wide range of applications in the technology-intensive industry that utilizes real-time vision machine-based detection technology, as well as in the industry where cutting-edge science and technology are converged and produced.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.42 no.2
• /
• pp.432-443
• /
• 2017

The primary goal of the MPEG-H 3D Audio standard is to provide immersive audio environments for high-resolution broadcasting services such as UHDTV. This standard incorporates a wide range of technologies such as encoding/decoding technology for multi-channel/object/scene-based signal, rendering technology for providing 3D audio in various playback environments, and post-processing technology. The reference software decoder of this standard is a structure combining several modules and can operate in various modes. Each module is composed of independent executable files and executed sequentially, real time decoding is impossible. In this paper, we make DLL library of the core decoder, format converter, object renderer, and binaural renderer of the standard and integrate them to enable frame-based decoding. In addition, by measuring the computation complexity of each mode of the MPEG-H 3D-Audio decoder, this paper also provides a reference for selecting the appropriate decoding mode for various hardware platforms. As a result of the computational complexity measurement, the low complexity profiles included in Korean broadcasting standard has a computation complexity of 2.8 times to 12.4 times that of the QMF synthesis operation in case of rendering as a channel signals, and it has a computation complexity of 4.1 times to 15.3 times of the QMF synthesis operation in case of rendering as a binaural signals.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.47 no.2
• /
• pp.107-121
• /
• 2021

Skin hypopigmentation, which is observed in albinism or vitiligo, occurs when melanin synthesis is decreased by genetic, epigenetic, and other factors. To identify drug candidates that can promote melanin synthesis in cells, we screened an epigenetic modulator library consisting of 141 cell-permeable, small molecule drugs. B16/F10 murine melanoma cells were treated with each drug at 0.1 𝜇M and melanin synthesis and cell viability were subsequently monitored. As a result, (-)-neplanocin A, 3-deazaneplanocin A (DZNep), and DZNep hydrochloride were found to increase cellular melanin synthesis without causing cytotoxicity. Because these three structurally related drugs exhibited similar dose-dependent effects on melanin synthesis and cell viability, DZNep was selected as a representative drug for additional experiments. DZNep increased intracellular melanin content and tyrosinase (TYR) activity. DZNep also induced the expression of TYR, tyrosinase-related protein 1 (TYRP1), and dopachrome tautomerase (DCT) at the mRNA and protein levels. DZNep also induced the mRNA and protein expression of microphthalmia-associated transcription factor (MITF), a key regulator of melanin synthesis. DZNep is a specific inhibitor of S-adenosylhomocysteine hydrolase and it caused the accumulation of S-adenosylhomocysteine that inhibits histone methyltransferases in cells. This study suggests that melanogenesis can be modulated by targeting S-adenosylhomocysteine hydrolase in certain cellular contexts.

• Journal of Korea Water Resources Association
• /
• v.47 no.3
• /
• pp.257-267
• /
• 2014

The impact of the combination of changes in temperature and rainfall due to climate change on surface water resources is important in hydro-meteorological research. In this study, 4 hydro-meteorological (HM) models from the Rainfall Runoff Library in the Catchment Modeling Toolkit were used to model the impact of climate change on runoff in streams for 5 river basins in the Republic of Korea. Future projections from 2021 to 2040 (2030s), 2051 to 2070 (2060s) and 2081 to 2099 (2090s), were derived from 12 General Circulation Models (GCMs) and 3 representative concentration pathways (RCPs). GCM outputs were statistically adjusted and downscaled using Long-Ashton Research Station Weather Generator (LARS-WG) and the HM models were well calibrated and verified for the period from 1999 to 2009. The study showed that there is substantial spatial, temporal and HM uncertainty in the future runoff shown by the interquartile range, range and coefficient of variation. In summary, the aggregated runoff will increase in the future by 10~24%, 7~30% and 11~30% of the respective baseline runoff for the RCP2.6, RCP4.5 and RCP8.5, respectively. This study presents a method to model future stream-flow taking into account the HM model and climate based uncertainty.