• International Journal of Knowledge Content Development & Technology
• /
• 제11권1호
• /
• pp.49-64
• /
• 2021

This research paper delved into the availability and utilization of library portal services for research in university libraries in Nigeria. Two research questions and two null hypotheses were formulated to guide the study. A structured questionnaire was used for data collection. The study was carried out in Nnamdi Azikiwe Library, University of Nigeria Nsukka (UNN) and Felix Aghagbo Nwako Library, Nnamdi Azikiwe University Library (UNIZIK), Awka. A total of 70 professionals (librarians and system analysts) in these libraries supplied data for the study. Data collected was analyzed using mean and standard deviation. These were used to answer the research questions while the null hypotheses were tested using t-test statistic at 0.05 level of significance and 68 degree of freedom. Major findings showed that portal services available in the library showed that the areas of availability of library portal services in Nnamdi Azikiwe Library, U.N.N. includes Agriculture, Health Sciences, Engineering, Environmental Sciences and information about institution, Social Sciences, Arts and Humanities, Natural Sciences. In Festus Aghagbo Nwako Library, Awka, the areas of Portal Library Services include Career Development, Federal Government Programs, Environmental Sciences, Biological Sciences, Social Sciences, Arts and Humanities, Engineering, and Health Sciences. Findings on extent of utilization of portal services available in the libraries indicated that portal services are utilized to a low extent in the university libraries. Furthermore, librarians did not significantly differ in their opinions on the availability of library portal services and on the extent of utilization of library portal services in the libraries under study. It is therefore recommended that provisions should be made by the universities and library management to provide and update required portal services in addition to creating enabling environment for enhanced access and utilization of these services. The governments must make an effort to provide funds for policy implementation, necessary technology training for librarians and users, and develop general information infrastructure.

• Entomological Research
• /
• 제48권5호
• /
• pp.384-389
• /
• 2018

Aphis gossypii Glover is an important insect pest that functions as a viral vector and mediates approximately 45 different viral diseases. As part of a strategy for control of A. gossypii, we investigated the functions of genes using RNAi. To this end, a cDNA library was constructed for various genes and for selecting appropriate targets for RNAi mediated silencing. The cDNA library was constructed using the Gateway cloning system with site-specific recombination of bacteriophage ${\lambda}$. It was used to carry out single step cloning of A. gossypii cDNAs. As a result, a cDNA library with a titer of $8.4{\times}10^6$ was constructed. Since the sequences in this library carry att sites, they can be cloned into various binary vectors. This library will be of value for various studies. For later screening of selected genes, it is planned to clone the library into virus-induced gene silencing (VIGS) vectors, which makes it possible to analyze gene function and allow subsequent transfection of plants. Such transfection experiments will allow testing of RNAi-induced insecticidal activity or repellent activity to A. gossypii, and result in the identification of target genes. It is also expected that the constructed cDNA library will be useful for analysis of gene functions in A. gossypii.

• Animal cells and systems
• /
• 제5권3호
• /
• pp.231-236
• /
• 2001

SINE-R retroposons have been derived from human endogenous retrovirus HERV-K family and found to be hominoid specific. Both SINE-R retroposons and HERV-K family are potentially capable of affecting the expression of closely located genes. From cDNA library of human fetal brain, we identified seven SINE-R retroposons and compared them with sequences derived from GenBank database. The SINE-R retroposons from human feta1 brain showed 85∼97% sequence similarities with the human-specific retroposon SINE-R.C2. They also showed 88∼96% sequence similarities with the sequence of the schizo-cDNA clone that derived from postmortem frontal cortex tissue of a schizophrenic patient. Phylogenetic analysis using the neiqhbor-joining method revealed that the seven new SINE-R retroposons from cDNA library of the human feta1 brain have proliferated independently during human evolution. The data indicate that such SINE-R retroposons are expressed in human fetal brain and deserve further investigation as potential leads to understanding of neuropsychiatric diseases.

• International Journal of Internet, Broadcasting and Communication
• /
• 제6권1호
• /
• pp.5-8
• /
• 2014

Social networking services that connect a person to other people are attracting our attention and various types of on-the-network services are provided. Library has been playing a role of social media by providing with materials such as books and magazines, and with a place for reading, studying, getting lectures, etc. In this paper, we present a method for finding candidates of groups of the library's patrons who share interest areas by utilizing the loan records, which are obtainable by every library. Such a homogeneous group can become a candidate for a study group, a community for exchange ideas, and other activity group. We apply the method to a collection of loan records of a university library, find some problem to be solved, and propose measures for more detailed solutions. Even though the potential group finding problem still remains a lot of problems to be solved, its potential importance is very high and thus to be studies even more for future applications.

• Molecules and Cells
• /
• 제27권2호
• /
• pp.225-235
• /
• 2009

Antibody phage display provides a powerful and efficient tool for the discovery and development of monoclonal antibodies for therapeutic and other applications. Antibody clones from synthetic libraries with optimized design features have several distinct advantages that include high stability, high levels of expression, and ease of downstream optimization and engineering. In this study, a fully synthetic human scFv library with six diversified CDRs was constructed by polymerase chain reaction assembly of overlapping oligonucleotides. In order to maximize the functional diversity of the library, a ${\beta}$-lactamase selection strategy was employed in which the assembled scFv gene repertoire was fused to the 5'-end of the ${\beta}$-lactamase gene, and in-frame scFv clones were enriched by carbenicillin selection. A final library with an estimated total diversity of $7.6{\times}10^9$, greater than 70% functional diversity, and diversification of all six CDRs was obtained after insertion of fully randomized CDR-H3 sequences into this proofread repertoire. The performance of the library was validated using a number of target antigens, against which multiple unique scFv sequences with dissociation constants in the nanomolar range were isolated.

• Journal of Veterinary Science
• /
• 제22권4호
• /
• pp.45.1-45.13
• /
• 2021

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 10⁶ cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

• 한국도서관정보학회지
• /
• 제24권
• /
• pp.107-161
• /
• 1996

This study aims at introducing 'ethnography' which is one of the most representative qualitative research methods into Library and Information Science research in Korea.. Ethnography, derived from anthropology, helps researchers to understand the whole and deep aspect of the research object. That is because the researcher puts himself into the life-world of the research object and observes it for a long time. Ethnography can be used as an alternative method to quantitative research methods. Until now, Library and Information Science research in Korea has used quantitative research methods in almost every case. From the 1980s so-called 'scientific methods' using hypotheses testing, have provided the major premise for research methodology in Library and Information Science of Korea. And the researchers have seen their research object(Korean Libraries) not in the native perspective but largely in the western(especially American) perspective. There is a need in Korea for more culturally relative research. So the desirability of introducing ethnography and other qualitative research methods into Library and Information Science research in Korea can be summarized as follows : I. Ethnography and other qualitative methods are needed for the researchers to overcome the limitation of quantitative methods which have formed the main methodological paradigm in Library and Information Science research in Korea. While those quantitative scientific methods can be a n.0, pplied to the social sciences, they are not adequate for the social sciences. It is because the research objects of the social sciences are human and social phenomena. II. It is needed that Library and Information Science research in Korea pay more attention to the speciality of Korean libraries. To do researches based on the viewpoint of cultural-relativism, researchers should consider the cultural context of Korean libraries. During the past years researchers in other social science fields in Korea, especially sociology and pedagogy, have gradually a n.0, pplied the methods of ethnography to their fields. These social scientists have attempted to escape from ethnocentrism, a problem which has greatly influenced past and present research methods. To get a holistic and in-depth understanding of Korean libraries on the present stage, and to solve their problems radically, it seems imperative that Library and Information Science research in Korea pay more attention to qualitative research methods such as ethnography.

• 한국문헌정보학회지
• /
• 제30권4호
• /
• pp.129-149
• /
• 1996

학문으로서의 문헌정보학은 1930년대부터 사회과학(또는 응용사회과학)의 한 분야로 출발하였다. 그러나 실무와 기술을 강조하는 기능 주의적 입장으로 인해 문헌정보학의 사회과학성은 점차 상실되었고, 대신 과학기술적인 입장이 강조되었다. 결국 문헌정보학은 주된 연구대상인 도서관과 그 관련기관을 사회적 지식체계로 파악하지 못하였으며, 사회과학적 이론과 철학을 도입하는 데도 소홀할 수밖에 업었다. 그러므로 이 연구에서는 현 단계 문헌정보학의 학문성에 대한 인식론적 전환을 위하여 도서관사회학이라는 연구영역을 시론 적으로 제시하려고 하였다. 이것은 문헌정보학의 본질을 사회과학에서 파악한 것으로서 그것을 뒷받침해 줄 수 있는 대표적인 인식론으로서 사회인식론과 지식사회학을 제시하고 이를 토대로 도서관사회학이라는 새로운 연구영역을 시론 적으로 모색하게 되었다.

• 한국통신학회논문지
• /
• 제42권5호
• /
• pp.1027-1037
• /
• 2017

CNG가 활용되는 환경이 증가함에 따라, CNG 암호 라이브러리에서의 보안 취약점 분석에 대한 연구가 요구되는 실정이다. 이에 본 논문에서는 CNG 암호 라이브러리에서의 SSL 통신과정을 분석함으로써 SSL 통신을 활용하는 응용에서 발생 가능한 보안 취약점을 도출하기 위한 자료 및 보안성을 향상시키는데 기여할 것으로 사료된다.

• Journal of Information Science Theory and Practice
• /
• 제8권1호
• /
• pp.45-55
• /
• 2020

The purpose of this work was to study library website users' actions by tracking their behavior, determining popular content, and identifying browsing patterns and subsequent improvement of access to popular content. The study of behavior models and the use of web analytics has led to the emergence of solutions that improve the usability and functionality of the State Public Scientific-Technological Library of the Siberian Branch of the Russian Academy of Sciences (SPSTL SB RAS) website. These are: identifying user tasks as they are developed, conducting user testing to better understand the event. tracking data and collecting additional data to verify the effectiveness of the changes made. Examining data on the duration of the session and the number of visits will help determine the goals of user visits and develop new recommendations. Usability analysis and testing will make it possible to compare the data obtained using web analytics and the perception of the library site by the users themselves. Recommendations are offered to libraries on the use of data on the real behavior of the target audience of the library website to improve access to library resources and services, increase their relevance and improve information services.