• 대한종양외과학회지
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• 제14권2호
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• pp.76-82
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• 2018

Purpose: Lgr5 is a well-known stem cell marker in colorectal cancer (CRC). This retrospective study evaluated the expressions of Lgr5 in CRC specimens, and examined whether these expressions were associated with survival outcomes. Methods: We used immunohistochemistry to retrospectively examine expressions of Lgr5 in paraffin-embedded specimens from 337 patients with CRC between January 2009 and December 2013. All clinicopathologic data were collected by retrospective review based on medical records. The correlation between its expression and clinicopathological data as well as clinical outcomes of patients was analyzed. Results: Low expression and high expression of Lgr5 in 337 patients were 175 (51.9%) and 162 (48.1%), respectively. There was no statistically significant difference in the association of Lgr5 expression with clinicopathologic factors (age, tumor location, lymphatic invasion, vascular invasion, perineural invasion, TNM stage, and differentiation). In the survival analysis, the high expression group of Lgr5 showed a better prognosis than the low expression group in disease-free survival (P=0.044). However, overall survival was not significantly different between the two groups (P=0.087). In multivariate analysis, we found that high expression of Lgr5 was independent prognostic factor for tumor relapse (hazard ratio, 0.601; 95% confidence interval, 0.388-0.929; P=0.022). Conclusion: In present study, high expression of Lgr5 is an independent predictor of favorable prognosis in patients with CRC. So, further well designed, prospective, large scale studies are needed to examine the value of Lgr5 as a prognostic biomarker for CRC.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4177-4183
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• 2015

Background: Gastric cancer is the second leading cause of cancer-related death in Asia, and the majority type is gastric adenocarcinoma (GAC). Most GAC patients die of recurrence and metastasis. Cancer stem cells (CSCs) have been thought to be responsible for the initiation, development, metastasis, and ultimately recurrence of cancer. In this study, we aimed to investigate expression and clinical significance of CSCs markers, CD133 and Lgr5, and vasculogenic mimicry (VM) in primary GAC. Materials and Methods: Specimens from 261 Chinese patients with follow-up were analyzed for CD133, Lgr5 protein expression and VM by immunohistochemical and histochemical staining. The Pearson Chi's square test was used to assess the associations among the positive staining of these markers and clinicopathological characteristics. Postoperative overall survival time was were studied by univariate and multivariate analyses. Results: In GAC tissues, positive rates of 49.0%, 38.7%, and 26.8% were obtained for CD133, Lgr5, and VM, respectively. The mean score of microvessel density (MVD) was $21.7{\pm}11.1$ in GAC tissues. There was a significantly difference between the positive and negative groups. There was a positive relationship between the VM, the expression of CD133 and Lgr5, and the score of MVD and the grades of tumor, lymph node metastasis, TNM stages (all p<0.05). The overall mean survival time of the patients with CD133, Lgr5, VM, and MVD (${\geq}22$) positive expression was lower than that of patients with negative expression. The score of MVD, positive expression of CD133 and VM were independent prognostic factors of GAC (p<0.05). Conclusions: VM, and expression of CD133, Lgr5, and the score of MVD are related to grades of tumor, lymph node metastasis, TNM stages, and overall mean survival time. It is suggested that CSCs and VM could play an important role in the evolution of GAC.

• 대한화장품학회지
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• 제49권1호
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• pp.75-85
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• 2023

본 연구에서는 7 개의 아미노산으로 이루어진 헵타펩타이드의 Lgr5 binding에 따른 인체 모낭 구성 세포의 활성에 대한 영향을 확인하였다. 표면 플라즈몬 공명(surface plasmon resonance, SPR) 시스템을 이용하여 헵타펩타이드가 Lgr5에 결합하는 것을 확인하였다. 인체 모유두세포(human hair follicle dermal papilla cell, HHFDPC)에 헵타펩타이드를 처리한 결과, 농도 의존적인 세포 증식이 나타났으며 β-catenin의 세포 내핵 이동 및 하위 유전자인 LEF1, Cyclin-D1, c-Myc의 발현 증가가 관찰되었다. 그리고 세포 증식 기전 관련 인자인 Akt와 ERK의 인산화 수준이 증가되었으며, 성장인자인 hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), vascular endothelial growth factor (VEGF) 발현이 유도되었다. 또한 인체 모모세포(human hair germinal matrix cell, HHGMC)의 분화 관련 전사 인자와 인체 외모근초세포(human hair outer root sheath cell, HHORSC)의 분화 표지 인자들도 헵타펩타이드 처리 시 높은 발현율을 보였다. 추가적으로 우리는 헵타펩타이드의 인체 모낭줄기세포(human hair follicle stem cell, HHFSC) 분화에 대한 영향을 조사하였다. 그 결과, HHFSC 표지인자들의 mRNA와 단백질 수준이 감소하였고 반면에 분화 표지인자들은 증가하였다. 상기의 결과들은 헵타펩타이드가 인체 모낭 구성 세포에서 Wnt/β-catenin 경로를 촉진시켜 증식 또는 분화를 유도할 수 있음을 보여준다. 이를 토대로 종합해 볼 때, 본 연구의 헵타펩타이드는 모발 성장을 유도하고 탈모 개선에 도움을 줄 수 있는 기능성 원료로 사용될 수 있을 것으로 보인다.

• 정보처리학회논문지:컴퓨터 및 통신 시스템
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• 제5권1호
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• pp.7-16
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• 2016

이 논문은 인터넷 최상위 한자 도메인의 국제 생성 규칙(LGR)을 위하여 검토 대상 이체자 묶음 선정 방안을 연구한다. 한자 도메인의 국제 생성 규칙을 정하는데 있어 가장 어려운 점은 한자 이체자를 어떻게 처리할 것인가이다. 만일 이체자가 들어간 도메인들(보기: 동해국(東海國), 동해국)이 각각 다른 주소로 접속된다면 혼란이 일어나므로 같은 주소로 접속되는 것이 바람직하다. 그런데 한중의 이체자 묶음이 같지 않으므로 한중의 이체자 묶음을 통합할 필요가 있다. 이런 이유로 저자는 중국 이체자 묶음 3093개에 대한 검토 작업을 진행하다가 한국 한자가 전혀 없거나 한 글자만 있는 중국 이체자 묶음은 한국이 검토할 필요가 없음을 발견하였다. 한국은 중국 이체자 묶음 가운데 한국 한자가 2자 이상 있는 것만 검토하면 된다. 이렇게 함으로써 저자는 한국이 검토해야 할 중국 이체자 묶음의 개수를 3093개에서 303개로 줄일 수 있었는데, 이는 원래 중국 이체자 묶음 개수의 1/10 이다. 본 논문에서 제안한 방법에 따라 중국 이체자 묶음 303개에 대한 검토가 마무리되면 중국과의 협의를 통해 인터넷 최상위 한자 도메인의 국제 생성 규칙을 정하는 작업이 가속화될 것이다.

• Journal of Gastric Cancer
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• 제8권4호
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• pp.225-231
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• 2008

목적: 위 점막하 종양(gastric submucosal tumor, gastric SMT)은 최근 건강검진의 보편화로 증가하는 추세에 있다. 이에 반해 악성도에 따라 예후가 달라지고, 방사선 검사나 내시경 조직검사로 다른 종양과의 감별이 어려워 아직도 치료 방침을 결정하는데 어려움이 있다. 이에 저자들은 위 점막하 종양 환자에서 임상병리학적 특성을 분석하고, 이에 따른 복강경 수술과 개복술의 결과를 각각 비교 분석하였다. 위 점막하종양의 복강경 절제술의 학습곡선 의미를 도출하여, 위 점막하종양의 적절한 치료방법을 제시하고자 한다. 대상 및 방법: 2003년 4월부터 2008년 8월까지 본원에서 위점막하종양으로 개복 위절제술을 받은 25명과 복강경 위절제술을 시행 받은 78명의 환자들을 대상으로 하였으며, 수술 후 임상경과 및 병리학적 특징에 대하여 전향적인 자료 수집 후 후향적으로 결과를 비교 분석하였다. 결과: 환자의 평균 연령은 53.7세(22~80세)였으며 남녀비는 각각 44명, 59명으로 1 : 1.34였다. 대부분 무증상으로 내원하였으며, 주로 쐐기형 위절제술을 시행 받았다. 종양의 평균 장경은 4.33 cm (1.2~17.0 cm)였고, 주로 위상부에 위치하였으며, 평균 수술 시간은 117.3분(35~255분)이었다. 평균 출혈량은 113.9 ml (0~1,000 ml), 평균 수술 후 재원기간은 8.0일(1~69일)이었다. 술 전 내시경적 절제술이 실패하여 수술이 의뢰된 환자가 5명 있었다. 수술 후 합병증은 13명(12.6%)에서 발생하였고, 수술과 관련된 사망은 없었다. 위 점막하종양의 대부분은 간질유래성증양으로 58명(56.3%)이며, 이중 현재까지 크기가 5 cm 이상이고 복강경 위절제술을 시행한 2명이 재발되었다. 복강경 수술 군과 개복 수술 군에 따라 수술 시간 및 출혈량, 합병증에는 차이가 없었지만, 두 군 간에 환자의 증상(P<0.001), 종양의 크기 (P<0.001), 수술방법 중 쐐기형 절제술 시행(P=0.037), 술 후 연식섭취 시간(P<0.001), 술 후 퇴원 일(P=0.002)에는 차이가 있었다. 위 점막하종양의 복강경 위절제술은 26예 이상의 수술 경험이 필요하고, 이러한 학습 곡선을 극복함으로써 수술 시간, 출혈량을 줄일 수 있다. 결론: 5 cm 미만의 위 점막하종양에서는 복강경적 치료방법이 개복술보다 안전하고, 효과적인 치료방법으로 생각되며, 다양한 형태의 위 점막하종양을 복강경적 절제술로 시행 할 경우에는 학습 곡선을 먼저 극복하기 위해 노력하는 것이 권장된다.

• 한국의상디자인학회지
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• 제14권4호
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• pp.63-72
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• 2012

In this study, Characteristics of colors on Chinese traditional costume in Tang, Song, Yuan, Ming, and Qing Dynasty were compared. And general colors of Chinese traditional costume and the viewpoint of them were considered. The purpose of this study were to apt colors of Chinese traditional costumes to the sensory appetite of modern people for new things, and make the recreation inspired by new senses be applied into the fashion industry effectively. The results were as followed : Firstly, colors distribution of 5 dynasties were different. In the Tang, R(red) was highest, YR(yellow red) and Y(yellow) followed. In the Song, YR was highest, Y and R followed and PB(purple blue) was increased, In the Yuan, R, YR, PB, B were concentrated at low rates, and other colors were increased, In the Ming, Y, R and B were concentrated at low rates orderly, and other colors were increased, In the Qing, PB was highest and Y, R, YR, B, P(purple) were increased. Secondly, tons distribution of 5 dynasties were different. In the Tang, L(light) was highest, P(pale) and Lgr(light grayish) followed. In the Song, P(pale) was highest, Lgr, Gr(grayish) and Dl(dull) followed In the Yuan, Dp(deep) was highest, P and Lgr followed, and other tones were increased, In the Ming, S(strong) was highest, DI and V(vivid) followed. Thirdly, The general colors of 5 dynasty were R, YR, Y, B, PB colors with vivid, deep and light tones. Forth, the viewpoint of color on Chinese traditional costume was based on the Yin-Yang and the Five Elements theory. Based on results of this study, the successive researches will be carried out about the unique colors of each nation and application traditional colors to modern fashion industry in order to the unique cultural sense.

• 한국재료학회지
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• 제29권10호
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• pp.579-585
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• 2019

In this study, we investigate the effect of Al/N source ratios and growth rates on the growth and structural properties of AlN films on c-plane sapphires by plasma-assisted molecular beam epitaxy. Both growth rates and Al/N ratios affect crystal qualities of AlN films. The full width at half maximum (FWHM) values of ($10{\bar{1}}5$) X-ray rocking curves (XRCs) change from 0.22 to $0.31^{\circ}$ with changing of the Al/N ratios, but the curves of (0002) XRCs change from 0.04 to $0.45^{\circ}$ with changing of the Al/N ratios. This means that structural deformation due to dislocations is slightly affected by the Al/N ratio in the ($10{\bar{1}}5$) XRCs but affected strongly for the (0002) XRCs. From the viewpoint of growth rate, the AlN films with high growth rate (HGR) show better crystal quality than the low growth rate (LGR) films overall, as shown by the FWHM values of the (0002) and ($10{\bar{1}}5$) XRCs. Based on cross-sectional transmission electron microscope observation, the HGR sample with an Al/N ratio of 3.1 shows more edge dislocations than there are screw and mixed dislocations in the LGR sample with Al/N ratio of 3.5.

• BMB Reports
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• 제55권7호
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• pp.348-353
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• 2022

Gastrointestinal cancer is associated with a high mortality rate. Here, we report that the splice variant of NRP/B contributes to tumorigenic activity in highly malignant gastric cancer through dissociation from the tumor repressor, HDAC5. NRP/B mRNA expression is significantly higher in the human gastric cancer tissues than in the normal tissues. Further, high levels of both the NRP/B splice variant and Lgr5, but not the full-length protein, are found in highly tumorigenic gastric tumor cells, but not in non-tumorigenic cells. The loss of NRP/B markedly inhibits cell migration and invasion, which reduces tumor formation in vivo. Importantly, the inhibition of alternative splicing increases the levels of NRP/B-1 mRNA and protein in AGS cells. The ectopic expression of full-length NRP/B exhibits tumor-suppressive activity, whereas NRP/B-2 induces the noninvasive human gastric cancer cells tumorigenesis. The splice variant NRP/B-2 which loses the capacity to interact with tumor repressors promoted oncogenic activity, suggesting that the BTB/POZ domain in the N-terminus has a crucial role in the suppression of gastric cancer. Therefore, the regulation of alternative splicing of the NRP/B gene is a potential novel target for the treatment of gastrointestinal cancer.

• 한국의류산업학회지
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• 제3권3호
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• pp.204-210
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• 2001

The purpose of the study is to clarify red image in the modem fashion. 40 kinds of costume samples being visual power in red have been selected from photographs in fashion magazines and divided into tones: pale (Vp, Lgr, L), bright (P, B), vivid (S, B, Dp), dark (Gr, Dl, Dgr, Dk). The study was measured by using Semantic Differential method. The subjects were 50 students majoring in clothing and textile. The data were analyzed by factor analysis, ANOVA, discrimminant analysis, MDS and regression analysis. The results of analysis are as follow; 1. Factor analysis has extracted 5 factors of red image in the fashion. These factor are Attractiveness, Hardness and Softness, Emotion, Attention, Simplicity. 2. There were significant difference in visual evaluation of red tones. 3. The discrimination among 4 red tones was related to attention and weight of red. 4. Evaluative dimensions of red was classified as Soft-Hard, Lively-Decent. 5. The image effect on Preference, Buying needs, Pleasant and Riches was consist of complicated sensibility.

• 패션비즈니스
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• 제6권4호
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• pp.163-172
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• 2002

The purpose of this study is to investigate purple image and formative properties in contemporary fashion. For the study of formative properties 152 kinds of costume samples from photographs in fashion magazines. For the study of image 40 kinds of costume samples is used and divided into tones : pale(Vp, Lgr, L), bright(P, B), vivid(S, V, Dp), dark(Gr, Dl, Dgr, Dk). A total 24 pairs of abjective words were used to measure the semantic differential scale in this study. The data were analyzed by factor analysis, ANOVA, MDS and regression analysis. The results are as follows; 1. Factor analysis has extracted 5 factors which consist of purple image in contemporary fashion. The factors are high quality, hardness and softness, simplicity, temperature, maturity. 2. There were significant differences in visual evaluation of purple tones. 3. The discrimination among. 4 purple tones was related to weight of purple 4. The evaluative dimensions of purple image in modern fashion were identified by Light - Heavy and Pale - Strong. 5. The image effect on Preference, Buying needs, Pleasant and Riches was consist of complicated sensibility.